Plastidial alpha-glucan phosphorylase is not required for starch degradation in Arabidopsis leaves but has a role in the tolerance of abiotic stress
To study the role of the plastidial alpha-glucan phosphorylase in starch metabolism in the leaves of Arabidopsis, two independent mutant lines containing T-DNA insertions within the phosphorylase gene were identified. Both insertions eliminate the activity of the plastidial alpha-glucan phosphorylas...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2004-06, Vol.135 (2), p.849-858 |
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| creator | Zeeman, S.C Thorneycroft, D Schupp, N Chapple, A Weck, M Dunstan, H Haldimann, P Bechtold, N Smith, A.M Smith, S.M |
| description | To study the role of the plastidial alpha-glucan phosphorylase in starch metabolism in the leaves of Arabidopsis, two independent mutant lines containing T-DNA insertions within the phosphorylase gene were identified. Both insertions eliminate the activity of the plastidial alpha-glucan phosphorylase. Measurement of other enzymes of starch metabolism reveals only minor changes compared with the wild type. The loss of plastidial alpha-glucan phosphorylase does not cause a significant change in the total accumulation of starch during the day or its remobilization at night. Starch structure and composition are unaltered. However, mutant plants display lesions on their leaves that are not seen on wild-type plants, and mesophyll cells bordering the lesions accumulate high levels of starch. Lesion formation is abolished by growing plants under 100% humidity in still air, but subsequent transfer to circulating air with lower humidity causes extensive wilting in the mutant leaves. Wilted sectors die, causing large lesions that are bordered by starch-accumulating cells. Similar lesions are caused by the application of acute salt stress to mature plants. We conclude that plastidial phosphorylase is not required for the degradation of starch, but that it plays a role in the capacity of the leaf lamina to endure a transient water deficit. |
| doi_str_mv | 10.1104/pp.103.032631 |
| format | Article |
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Both insertions eliminate the activity of the plastidial alpha-glucan phosphorylase. Measurement of other enzymes of starch metabolism reveals only minor changes compared with the wild type. The loss of plastidial alpha-glucan phosphorylase does not cause a significant change in the total accumulation of starch during the day or its remobilization at night. Starch structure and composition are unaltered. However, mutant plants display lesions on their leaves that are not seen on wild-type plants, and mesophyll cells bordering the lesions accumulate high levels of starch. Lesion formation is abolished by growing plants under 100% humidity in still air, but subsequent transfer to circulating air with lower humidity causes extensive wilting in the mutant leaves. Wilted sectors die, causing large lesions that are bordered by starch-accumulating cells. Similar lesions are caused by the application of acute salt stress to mature plants. We conclude that plastidial phosphorylase is not required for the degradation of starch, but that it plays a role in the capacity of the leaf lamina to endure a transient water deficit.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.103.032631</identifier><identifier>PMID: 15173560</identifier><language>eng</language><publisher>United States: American Society of Plant Biologists</publisher><subject>abiotic stress ; Adaptation, Physiological - drug effects ; Adaptation, Physiological - physiology ; alpha-glucan phosphorylase ; Arabidopsis - drug effects ; Arabidopsis - enzymology ; Arabidopsis - genetics ; Arabidopsis thaliana ; bioaccumulation ; Biochemical Processes and Macromolecular Structures ; carbohydrate content ; carbohydrate metabolism ; Chloroplasts ; Chloroplasts - enzymology ; degradation ; enzyme activity ; Enzymes ; Glucans ; Humidity ; Leaves ; Lesions ; lesions (plant) ; molecular sequence data ; Mutation ; nucleotide sequences ; phenotypic variation ; phosphorylase ; Phosphorylases - metabolism ; Plant cells ; Plant Leaves - drug effects ; Plant Leaves - enzymology ; plant morphology ; plant response ; Plants ; plastids ; Protein isoforms ; starch ; Starch - chemistry ; Starch - metabolism ; Starch Phosphorylase - metabolism ; Starches ; stress tolerance ; Stress, Mechanical ; transgenic plants ; Water - pharmacology ; water stress</subject><ispartof>Plant physiology (Bethesda), 2004-06, Vol.135 (2), p.849-858</ispartof><rights>Copyright 2004 American Society of Plant Biologists</rights><rights>Copyright © 2004, American Society of Plant Biologists 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-db925bff068cc661eed87422f3efb61243abffa6964f22001ee88fb04e18104f3</citedby><cites>FETCH-LOGICAL-c471t-db925bff068cc661eed87422f3efb61243abffa6964f22001ee88fb04e18104f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4281805$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4281805$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,803,885,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15173560$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zeeman, S.C</creatorcontrib><creatorcontrib>Thorneycroft, D</creatorcontrib><creatorcontrib>Schupp, N</creatorcontrib><creatorcontrib>Chapple, A</creatorcontrib><creatorcontrib>Weck, M</creatorcontrib><creatorcontrib>Dunstan, H</creatorcontrib><creatorcontrib>Haldimann, P</creatorcontrib><creatorcontrib>Bechtold, N</creatorcontrib><creatorcontrib>Smith, A.M</creatorcontrib><creatorcontrib>Smith, S.M</creatorcontrib><title>Plastidial alpha-glucan phosphorylase is not required for starch degradation in Arabidopsis leaves but has a role in the tolerance of abiotic stress</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>To study the role of the plastidial alpha-glucan phosphorylase in starch metabolism in the leaves of Arabidopsis, two independent mutant lines containing T-DNA insertions within the phosphorylase gene were identified. Both insertions eliminate the activity of the plastidial alpha-glucan phosphorylase. Measurement of other enzymes of starch metabolism reveals only minor changes compared with the wild type. The loss of plastidial alpha-glucan phosphorylase does not cause a significant change in the total accumulation of starch during the day or its remobilization at night. Starch structure and composition are unaltered. However, mutant plants display lesions on their leaves that are not seen on wild-type plants, and mesophyll cells bordering the lesions accumulate high levels of starch. Lesion formation is abolished by growing plants under 100% humidity in still air, but subsequent transfer to circulating air with lower humidity causes extensive wilting in the mutant leaves. Wilted sectors die, causing large lesions that are bordered by starch-accumulating cells. Similar lesions are caused by the application of acute salt stress to mature plants. We conclude that plastidial phosphorylase is not required for the degradation of starch, but that it plays a role in the capacity of the leaf lamina to endure a transient water deficit.</description><subject>abiotic stress</subject><subject>Adaptation, Physiological - drug effects</subject><subject>Adaptation, Physiological - physiology</subject><subject>alpha-glucan phosphorylase</subject><subject>Arabidopsis - drug effects</subject><subject>Arabidopsis - enzymology</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis thaliana</subject><subject>bioaccumulation</subject><subject>Biochemical Processes and Macromolecular Structures</subject><subject>carbohydrate content</subject><subject>carbohydrate metabolism</subject><subject>Chloroplasts</subject><subject>Chloroplasts - enzymology</subject><subject>degradation</subject><subject>enzyme activity</subject><subject>Enzymes</subject><subject>Glucans</subject><subject>Humidity</subject><subject>Leaves</subject><subject>Lesions</subject><subject>lesions (plant)</subject><subject>molecular sequence data</subject><subject>Mutation</subject><subject>nucleotide sequences</subject><subject>phenotypic variation</subject><subject>phosphorylase</subject><subject>Phosphorylases - metabolism</subject><subject>Plant cells</subject><subject>Plant Leaves - drug effects</subject><subject>Plant Leaves - enzymology</subject><subject>plant morphology</subject><subject>plant response</subject><subject>Plants</subject><subject>plastids</subject><subject>Protein isoforms</subject><subject>starch</subject><subject>Starch - chemistry</subject><subject>Starch - metabolism</subject><subject>Starch Phosphorylase - metabolism</subject><subject>Starches</subject><subject>stress tolerance</subject><subject>Stress, Mechanical</subject><subject>transgenic plants</subject><subject>Water - pharmacology</subject><subject>water stress</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUtv1DAUhS0EokNhyQ6BV-wy-BWPZ8GiqnhJlUCCrq0b53riKhOntlOp_4MfjKuMCiwsX_l859rXh5DXnG05Z-rDPG85k1smhZb8CdnwVopGtMo8JRtWTxtmzP6MvMj5hjHGJVfPyRlv-U62mm3I7x8j5BL6ACOFcR6gOYyLg4nOQ8x1pfuqIw2ZTrHQhLdLSNhTHxPNBZIbaI-HBD2UECcaJnqRoAt9nHO1jAh3mGm3FDpApkBTHPEBKgPSUusEk0MaPa2eWIKrPRPm_JI88zBmfHXaz8n150-_Lr82V9-_fLu8uGqc2vHS9N1etJ33TBvntOaIvdkpIbxE32kulISqgt5r5YWowyMa4zumkJv6dV6ek49r33npjtg7nEqC0c4pHCHd2wjB_q9MYbCHeGdbrrhg1f_-5E_xdsFc7DFkh-MIE8YlW61122ohK9isoEsx54T-8Q7O7EOMdp5rKe0aY-Xf_vuwv_Qptwq8WYGbXGJ61JUw3LC2yu9W2UO0cEgh2-ufoqbP2F7W2Vv5ByRgr54</recordid><startdate>20040601</startdate><enddate>20040601</enddate><creator>Zeeman, S.C</creator><creator>Thorneycroft, D</creator><creator>Schupp, N</creator><creator>Chapple, A</creator><creator>Weck, M</creator><creator>Dunstan, H</creator><creator>Haldimann, P</creator><creator>Bechtold, N</creator><creator>Smith, A.M</creator><creator>Smith, S.M</creator><general>American Society of Plant Biologists</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20040601</creationdate><title>Plastidial alpha-glucan phosphorylase is not required for starch degradation in Arabidopsis leaves but has a role in the tolerance of abiotic stress</title><author>Zeeman, S.C ; Thorneycroft, D ; Schupp, N ; Chapple, A ; Weck, M ; Dunstan, H ; Haldimann, P ; Bechtold, N ; Smith, A.M ; Smith, S.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-db925bff068cc661eed87422f3efb61243abffa6964f22001ee88fb04e18104f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>abiotic stress</topic><topic>Adaptation, Physiological - drug effects</topic><topic>Adaptation, Physiological - physiology</topic><topic>alpha-glucan phosphorylase</topic><topic>Arabidopsis - drug effects</topic><topic>Arabidopsis - enzymology</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis thaliana</topic><topic>bioaccumulation</topic><topic>Biochemical Processes and Macromolecular Structures</topic><topic>carbohydrate content</topic><topic>carbohydrate metabolism</topic><topic>Chloroplasts</topic><topic>Chloroplasts - enzymology</topic><topic>degradation</topic><topic>enzyme activity</topic><topic>Enzymes</topic><topic>Glucans</topic><topic>Humidity</topic><topic>Leaves</topic><topic>Lesions</topic><topic>lesions (plant)</topic><topic>molecular sequence data</topic><topic>Mutation</topic><topic>nucleotide sequences</topic><topic>phenotypic variation</topic><topic>phosphorylase</topic><topic>Phosphorylases - metabolism</topic><topic>Plant cells</topic><topic>Plant Leaves - drug effects</topic><topic>Plant Leaves - enzymology</topic><topic>plant morphology</topic><topic>plant response</topic><topic>Plants</topic><topic>plastids</topic><topic>Protein isoforms</topic><topic>starch</topic><topic>Starch - chemistry</topic><topic>Starch - metabolism</topic><topic>Starch Phosphorylase - metabolism</topic><topic>Starches</topic><topic>stress tolerance</topic><topic>Stress, Mechanical</topic><topic>transgenic plants</topic><topic>Water - pharmacology</topic><topic>water stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeeman, S.C</creatorcontrib><creatorcontrib>Thorneycroft, D</creatorcontrib><creatorcontrib>Schupp, N</creatorcontrib><creatorcontrib>Chapple, A</creatorcontrib><creatorcontrib>Weck, M</creatorcontrib><creatorcontrib>Dunstan, H</creatorcontrib><creatorcontrib>Haldimann, P</creatorcontrib><creatorcontrib>Bechtold, N</creatorcontrib><creatorcontrib>Smith, A.M</creatorcontrib><creatorcontrib>Smith, S.M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeeman, S.C</au><au>Thorneycroft, D</au><au>Schupp, N</au><au>Chapple, A</au><au>Weck, M</au><au>Dunstan, H</au><au>Haldimann, P</au><au>Bechtold, N</au><au>Smith, A.M</au><au>Smith, S.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plastidial alpha-glucan phosphorylase is not required for starch degradation in Arabidopsis leaves but has a role in the tolerance of abiotic stress</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2004-06-01</date><risdate>2004</risdate><volume>135</volume><issue>2</issue><spage>849</spage><epage>858</epage><pages>849-858</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><abstract>To study the role of the plastidial alpha-glucan phosphorylase in starch metabolism in the leaves of Arabidopsis, two independent mutant lines containing T-DNA insertions within the phosphorylase gene were identified. Both insertions eliminate the activity of the plastidial alpha-glucan phosphorylase. Measurement of other enzymes of starch metabolism reveals only minor changes compared with the wild type. The loss of plastidial alpha-glucan phosphorylase does not cause a significant change in the total accumulation of starch during the day or its remobilization at night. Starch structure and composition are unaltered. However, mutant plants display lesions on their leaves that are not seen on wild-type plants, and mesophyll cells bordering the lesions accumulate high levels of starch. Lesion formation is abolished by growing plants under 100% humidity in still air, but subsequent transfer to circulating air with lower humidity causes extensive wilting in the mutant leaves. Wilted sectors die, causing large lesions that are bordered by starch-accumulating cells. Similar lesions are caused by the application of acute salt stress to mature plants. We conclude that plastidial phosphorylase is not required for the degradation of starch, but that it plays a role in the capacity of the leaf lamina to endure a transient water deficit.</abstract><cop>United States</cop><pub>American Society of Plant Biologists</pub><pmid>15173560</pmid><doi>10.1104/pp.103.032631</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | abiotic stress Adaptation, Physiological - drug effects Adaptation, Physiological - physiology alpha-glucan phosphorylase Arabidopsis - drug effects Arabidopsis - enzymology Arabidopsis - genetics Arabidopsis thaliana bioaccumulation Biochemical Processes and Macromolecular Structures carbohydrate content carbohydrate metabolism Chloroplasts Chloroplasts - enzymology degradation enzyme activity Enzymes Glucans Humidity Leaves Lesions lesions (plant) molecular sequence data Mutation nucleotide sequences phenotypic variation phosphorylase Phosphorylases - metabolism Plant cells Plant Leaves - drug effects Plant Leaves - enzymology plant morphology plant response Plants plastids Protein isoforms starch Starch - chemistry Starch - metabolism Starch Phosphorylase - metabolism Starches stress tolerance Stress, Mechanical transgenic plants Water - pharmacology water stress |
| title | Plastidial alpha-glucan phosphorylase is not required for starch degradation in Arabidopsis leaves but has a role in the tolerance of abiotic stress |
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