Design and mechanistic insight into ultrafast calcium indicators for monitoring intracellular calcium dynamics

Calmodulin-based genetically encoded fluorescent calcium indicators (GCaMP-s) are powerful tools of imaging calcium dynamics from cells to freely moving animals. High affinity indicators with slow kinetics however distort the temporal profile of calcium transients. Here we report the development of...

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Veröffentlicht in:	Scientific reports 2016-12, Vol.6 (1), p.38276-38276, Article 38276
Hauptverfasser:	Helassa, Nordine, Podor, Borbala, Fine, Alan, Török, Katalin
Format:	Artikel
Sprache:	eng
Schlagworte:	631/378 631/57 82/80 82/83 9/10 96/35 Action Potentials - physiology Affinity Animals Binding Sites CA1 Region, Hippocampal - cytology CA1 Region, Hippocampal - metabolism Calcium Calcium (intracellular) Calcium - metabolism Calcium imaging Calcium Signaling Calcium signalling Calcium-binding protein Calmodulin Calmodulin - chemistry Calmodulin - genetics Calmodulin - metabolism Decay Dendrites - metabolism Design Electric Stimulation Equilibrium Fluorescent Dyes - chemistry Fluorescent Dyes - metabolism Gene Expression Genes, Reporter Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism HEK293 Cells Hippocampus Humanities and Social Sciences Humans Intracellular Kinetics multidisciplinary Mutation Peptides Peptides - chemistry Peptides - genetics Peptides - metabolism Protein Binding Protein Conformation, alpha-Helical Protein Conformation, beta-Strand Pyramidal cells Pyramidal Cells - cytology Pyramidal Cells - metabolism Rats, Sprague-Dawley Science Tissue Culture Techniques
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