γ-H2AX/53BP1/pKAP-1 foci and their linear tracks induced by in vitro exposure to radon and its progeny in human peripheral blood lymphocytes
The biodosimetric information is critical for evaluating the human health hazards caused by radon and its progeny. Here, we demonstrated that the formation of phosphorylated histone variant H2AX (γ-H2AX), p53-binding protein 1 (53BP1) and phosphorylated KRAB-associated protein 1 (pKAP-1) foci and th...
Gespeichert in:
| Veröffentlicht in: | Scientific reports 2016-12, Vol.6 (1), p.38295-38295, Article 38295 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 38295 |
|---|---|
| container_issue | 1 |
| container_start_page | 38295 |
| container_title | Scientific reports |
| container_volume | 6 |
| creator | Ding, Defang Zhang, Yaping Wang, Jing Wang, Xufei Fan, Dunhuang He, Linfeng Zhang, Xuxia Gao, Yun Li, Qiang Chen, Honghong |
| description | The biodosimetric information is critical for evaluating the human health hazards caused by radon and its progeny. Here, we demonstrated that the formation of phosphorylated histone variant H2AX (γ-H2AX), p53-binding protein 1 (53BP1) and phosphorylated KRAB-associated protein 1 (pKAP-1) foci and their linear tracks in human peripheral blood lymphocytes (HPBLs)
in vitro
exposed to radon and its progeny were dependent on the cumulative absorbed dose of radon exposure but was unrelated to the concentration of radon. Among them, γ-H2AX foci and its linear tracks were the most sensitive indicators with the lowest estimable cumulative absorbed dose of 1.74 mGy from their linear dose-response curves and sustained for 12 h after termination of radon exposure. In addition, three types of foci showed an overdispersed non-Poisson distribution in HPBLs. The ratios of pKAP-1/γ-H2AX foci co-localization, 53BP1/γ-H2AX foci co-localization and 53BP1/pKAP-1 foci co-localization were significantly increased in HPBLs exposed to radon while they were unrelated to the cumulative dose of radon exposure, suggesting that γ-H2AX, pKAP-1 and 53BP1 play an important role in the repair of heterochromatic double-strand breaks. Altogether, our findings provide an experimental basis for estimating the biological dose of internal α-particle irradiation from radon and its progeny exposure in humans. |
| doi_str_mv | 10.1038/srep38295 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5138821</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1846368480</sourcerecordid><originalsourceid>FETCH-LOGICAL-c410t-edc15518c4902b7cb83ab5a9e412ccf14363eb8a32faaf9c3fccb938fb31ce733</originalsourceid><addsrcrecordid>eNptkU9O3DAUhy1UBIiy4ALIyxYpHf9JZpxNpQG1pSoSLKjUnWU7LxPTxHbtBDGH6Gl6j56pLgMjkPDGT3qfv2f7h9AxJR8o4WKWIgQuWF3toANGyqpgnLE3z-p9dJTSLcmrYnVJ6z20zxY1Y5SSA_T775_igi1_zCp-dk1n4dvyuqC49cZi5Ro8dmAj7q0DFfEYlfmZsHXNZKDBep1LfGfH6DHcB5-mCHj0OKrGu4fTdkw4RL8C94B206AcDhBt6CCqHuve-wb36yF03qxHSG_Rbqv6BEeP-yH6_vnTzflFcXn15ev58rIwJSVjAY2hVUWFKWvC9MJowZWuVA0lZca0tORzDloozlql2trw1hhdc9FqTg0sOD9EHzfeMOkh28Dlt_UyRDuouJZeWfmy42wnV_5OVpQLwWgWvHsURP9rgjTKwSYDfa8c-ClJKso5n4tSkIy-36Am-pTDardjKJH_E5TbBDN78vxeW_IprwycboCUW24FUd76Kbr8V6_Y_gHWCahb</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1846368480</pqid></control><display><type>article</type><title>γ-H2AX/53BP1/pKAP-1 foci and their linear tracks induced by in vitro exposure to radon and its progeny in human peripheral blood lymphocytes</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Nature Free</source><source>PubMed Central</source><source>Springer Nature OA/Free Journals</source><source>Free Full-Text Journals in Chemistry</source><creator>Ding, Defang ; Zhang, Yaping ; Wang, Jing ; Wang, Xufei ; Fan, Dunhuang ; He, Linfeng ; Zhang, Xuxia ; Gao, Yun ; Li, Qiang ; Chen, Honghong</creator><creatorcontrib>Ding, Defang ; Zhang, Yaping ; Wang, Jing ; Wang, Xufei ; Fan, Dunhuang ; He, Linfeng ; Zhang, Xuxia ; Gao, Yun ; Li, Qiang ; Chen, Honghong</creatorcontrib><description>The biodosimetric information is critical for evaluating the human health hazards caused by radon and its progeny. Here, we demonstrated that the formation of phosphorylated histone variant H2AX (γ-H2AX), p53-binding protein 1 (53BP1) and phosphorylated KRAB-associated protein 1 (pKAP-1) foci and their linear tracks in human peripheral blood lymphocytes (HPBLs)
in vitro
exposed to radon and its progeny were dependent on the cumulative absorbed dose of radon exposure but was unrelated to the concentration of radon. Among them, γ-H2AX foci and its linear tracks were the most sensitive indicators with the lowest estimable cumulative absorbed dose of 1.74 mGy from their linear dose-response curves and sustained for 12 h after termination of radon exposure. In addition, three types of foci showed an overdispersed non-Poisson distribution in HPBLs. The ratios of pKAP-1/γ-H2AX foci co-localization, 53BP1/γ-H2AX foci co-localization and 53BP1/pKAP-1 foci co-localization were significantly increased in HPBLs exposed to radon while they were unrelated to the cumulative dose of radon exposure, suggesting that γ-H2AX, pKAP-1 and 53BP1 play an important role in the repair of heterochromatic double-strand breaks. Altogether, our findings provide an experimental basis for estimating the biological dose of internal α-particle irradiation from radon and its progeny exposure in humans.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep38295</identifier><identifier>PMID: 27922110</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13 ; 13/51 ; 692/1537 ; 692/53/2423 ; Air Pollutants, Radioactive - pharmacology ; DNA - genetics ; DNA - metabolism ; DNA Breaks, Double-Stranded ; DNA Repair ; Dose-Response Relationship, Radiation ; Histones - genetics ; Histones - metabolism ; Humanities and Social Sciences ; Humans ; Leukocytes, Mononuclear - metabolism ; Leukocytes, Mononuclear - radiation effects ; multidisciplinary ; Phosphorylation - radiation effects ; Primary Cell Culture ; Radiometry ; Radon - pharmacology ; Science ; Tripartite Motif-Containing Protein 28 - genetics ; Tripartite Motif-Containing Protein 28 - metabolism ; Tumor Suppressor p53-Binding Protein 1 - genetics ; Tumor Suppressor p53-Binding Protein 1 - metabolism</subject><ispartof>Scientific reports, 2016-12, Vol.6 (1), p.38295-38295, Article 38295</ispartof><rights>The Author(s) 2016</rights><rights>Copyright © 2016, The Author(s) 2016 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-edc15518c4902b7cb83ab5a9e412ccf14363eb8a32faaf9c3fccb938fb31ce733</citedby><cites>FETCH-LOGICAL-c410t-edc15518c4902b7cb83ab5a9e412ccf14363eb8a32faaf9c3fccb938fb31ce733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5138821/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5138821/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27923,27924,41119,42188,51575,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27922110$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ding, Defang</creatorcontrib><creatorcontrib>Zhang, Yaping</creatorcontrib><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Wang, Xufei</creatorcontrib><creatorcontrib>Fan, Dunhuang</creatorcontrib><creatorcontrib>He, Linfeng</creatorcontrib><creatorcontrib>Zhang, Xuxia</creatorcontrib><creatorcontrib>Gao, Yun</creatorcontrib><creatorcontrib>Li, Qiang</creatorcontrib><creatorcontrib>Chen, Honghong</creatorcontrib><title>γ-H2AX/53BP1/pKAP-1 foci and their linear tracks induced by in vitro exposure to radon and its progeny in human peripheral blood lymphocytes</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>The biodosimetric information is critical for evaluating the human health hazards caused by radon and its progeny. Here, we demonstrated that the formation of phosphorylated histone variant H2AX (γ-H2AX), p53-binding protein 1 (53BP1) and phosphorylated KRAB-associated protein 1 (pKAP-1) foci and their linear tracks in human peripheral blood lymphocytes (HPBLs)
in vitro
exposed to radon and its progeny were dependent on the cumulative absorbed dose of radon exposure but was unrelated to the concentration of radon. Among them, γ-H2AX foci and its linear tracks were the most sensitive indicators with the lowest estimable cumulative absorbed dose of 1.74 mGy from their linear dose-response curves and sustained for 12 h after termination of radon exposure. In addition, three types of foci showed an overdispersed non-Poisson distribution in HPBLs. The ratios of pKAP-1/γ-H2AX foci co-localization, 53BP1/γ-H2AX foci co-localization and 53BP1/pKAP-1 foci co-localization were significantly increased in HPBLs exposed to radon while they were unrelated to the cumulative dose of radon exposure, suggesting that γ-H2AX, pKAP-1 and 53BP1 play an important role in the repair of heterochromatic double-strand breaks. Altogether, our findings provide an experimental basis for estimating the biological dose of internal α-particle irradiation from radon and its progeny exposure in humans.</description><subject>13</subject><subject>13/51</subject><subject>692/1537</subject><subject>692/53/2423</subject><subject>Air Pollutants, Radioactive - pharmacology</subject><subject>DNA - genetics</subject><subject>DNA - metabolism</subject><subject>DNA Breaks, Double-Stranded</subject><subject>DNA Repair</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Histones - genetics</subject><subject>Histones - metabolism</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Leukocytes, Mononuclear - radiation effects</subject><subject>multidisciplinary</subject><subject>Phosphorylation - radiation effects</subject><subject>Primary Cell Culture</subject><subject>Radiometry</subject><subject>Radon - pharmacology</subject><subject>Science</subject><subject>Tripartite Motif-Containing Protein 28 - genetics</subject><subject>Tripartite Motif-Containing Protein 28 - metabolism</subject><subject>Tumor Suppressor p53-Binding Protein 1 - genetics</subject><subject>Tumor Suppressor p53-Binding Protein 1 - metabolism</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNptkU9O3DAUhy1UBIiy4ALIyxYpHf9JZpxNpQG1pSoSLKjUnWU7LxPTxHbtBDGH6Gl6j56pLgMjkPDGT3qfv2f7h9AxJR8o4WKWIgQuWF3toANGyqpgnLE3z-p9dJTSLcmrYnVJ6z20zxY1Y5SSA_T775_igi1_zCp-dk1n4dvyuqC49cZi5Ro8dmAj7q0DFfEYlfmZsHXNZKDBep1LfGfH6DHcB5-mCHj0OKrGu4fTdkw4RL8C94B206AcDhBt6CCqHuve-wb36yF03qxHSG_Rbqv6BEeP-yH6_vnTzflFcXn15ev58rIwJSVjAY2hVUWFKWvC9MJowZWuVA0lZca0tORzDloozlql2trw1hhdc9FqTg0sOD9EHzfeMOkh28Dlt_UyRDuouJZeWfmy42wnV_5OVpQLwWgWvHsURP9rgjTKwSYDfa8c-ClJKso5n4tSkIy-36Am-pTDardjKJH_E5TbBDN78vxeW_IprwycboCUW24FUd76Kbr8V6_Y_gHWCahb</recordid><startdate>20161206</startdate><enddate>20161206</enddate><creator>Ding, Defang</creator><creator>Zhang, Yaping</creator><creator>Wang, Jing</creator><creator>Wang, Xufei</creator><creator>Fan, Dunhuang</creator><creator>He, Linfeng</creator><creator>Zhang, Xuxia</creator><creator>Gao, Yun</creator><creator>Li, Qiang</creator><creator>Chen, Honghong</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20161206</creationdate><title>γ-H2AX/53BP1/pKAP-1 foci and their linear tracks induced by in vitro exposure to radon and its progeny in human peripheral blood lymphocytes</title><author>Ding, Defang ; Zhang, Yaping ; Wang, Jing ; Wang, Xufei ; Fan, Dunhuang ; He, Linfeng ; Zhang, Xuxia ; Gao, Yun ; Li, Qiang ; Chen, Honghong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-edc15518c4902b7cb83ab5a9e412ccf14363eb8a32faaf9c3fccb938fb31ce733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>13</topic><topic>13/51</topic><topic>692/1537</topic><topic>692/53/2423</topic><topic>Air Pollutants, Radioactive - pharmacology</topic><topic>DNA - genetics</topic><topic>DNA - metabolism</topic><topic>DNA Breaks, Double-Stranded</topic><topic>DNA Repair</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Histones - genetics</topic><topic>Histones - metabolism</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Leukocytes, Mononuclear - radiation effects</topic><topic>multidisciplinary</topic><topic>Phosphorylation - radiation effects</topic><topic>Primary Cell Culture</topic><topic>Radiometry</topic><topic>Radon - pharmacology</topic><topic>Science</topic><topic>Tripartite Motif-Containing Protein 28 - genetics</topic><topic>Tripartite Motif-Containing Protein 28 - metabolism</topic><topic>Tumor Suppressor p53-Binding Protein 1 - genetics</topic><topic>Tumor Suppressor p53-Binding Protein 1 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ding, Defang</creatorcontrib><creatorcontrib>Zhang, Yaping</creatorcontrib><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Wang, Xufei</creatorcontrib><creatorcontrib>Fan, Dunhuang</creatorcontrib><creatorcontrib>He, Linfeng</creatorcontrib><creatorcontrib>Zhang, Xuxia</creatorcontrib><creatorcontrib>Gao, Yun</creatorcontrib><creatorcontrib>Li, Qiang</creatorcontrib><creatorcontrib>Chen, Honghong</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ding, Defang</au><au>Zhang, Yaping</au><au>Wang, Jing</au><au>Wang, Xufei</au><au>Fan, Dunhuang</au><au>He, Linfeng</au><au>Zhang, Xuxia</au><au>Gao, Yun</au><au>Li, Qiang</au><au>Chen, Honghong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>γ-H2AX/53BP1/pKAP-1 foci and their linear tracks induced by in vitro exposure to radon and its progeny in human peripheral blood lymphocytes</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-12-06</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>38295</spage><epage>38295</epage><pages>38295-38295</pages><artnum>38295</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>The biodosimetric information is critical for evaluating the human health hazards caused by radon and its progeny. Here, we demonstrated that the formation of phosphorylated histone variant H2AX (γ-H2AX), p53-binding protein 1 (53BP1) and phosphorylated KRAB-associated protein 1 (pKAP-1) foci and their linear tracks in human peripheral blood lymphocytes (HPBLs)
in vitro
exposed to radon and its progeny were dependent on the cumulative absorbed dose of radon exposure but was unrelated to the concentration of radon. Among them, γ-H2AX foci and its linear tracks were the most sensitive indicators with the lowest estimable cumulative absorbed dose of 1.74 mGy from their linear dose-response curves and sustained for 12 h after termination of radon exposure. In addition, three types of foci showed an overdispersed non-Poisson distribution in HPBLs. The ratios of pKAP-1/γ-H2AX foci co-localization, 53BP1/γ-H2AX foci co-localization and 53BP1/pKAP-1 foci co-localization were significantly increased in HPBLs exposed to radon while they were unrelated to the cumulative dose of radon exposure, suggesting that γ-H2AX, pKAP-1 and 53BP1 play an important role in the repair of heterochromatic double-strand breaks. Altogether, our findings provide an experimental basis for estimating the biological dose of internal α-particle irradiation from radon and its progeny exposure in humans.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27922110</pmid><doi>10.1038/srep38295</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2016-12, Vol.6 (1), p.38295-38295, Article 38295 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5138821 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Nature Free; PubMed Central; Springer Nature OA/Free Journals; Free Full-Text Journals in Chemistry |
| subjects | 13 13/51 692/1537 692/53/2423 Air Pollutants, Radioactive - pharmacology DNA - genetics DNA - metabolism DNA Breaks, Double-Stranded DNA Repair Dose-Response Relationship, Radiation Histones - genetics Histones - metabolism Humanities and Social Sciences Humans Leukocytes, Mononuclear - metabolism Leukocytes, Mononuclear - radiation effects multidisciplinary Phosphorylation - radiation effects Primary Cell Culture Radiometry Radon - pharmacology Science Tripartite Motif-Containing Protein 28 - genetics Tripartite Motif-Containing Protein 28 - metabolism Tumor Suppressor p53-Binding Protein 1 - genetics Tumor Suppressor p53-Binding Protein 1 - metabolism |
| title | γ-H2AX/53BP1/pKAP-1 foci and their linear tracks induced by in vitro exposure to radon and its progeny in human peripheral blood lymphocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T17%3A13%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=%CE%B3-H2AX/53BP1/pKAP-1%20foci%20and%20their%20linear%20tracks%20induced%20by%20in%20vitro%20exposure%20to%20radon%20and%20its%20progeny%20in%20human%20peripheral%20blood%20lymphocytes&rft.jtitle=Scientific%20reports&rft.au=Ding,%20Defang&rft.date=2016-12-06&rft.volume=6&rft.issue=1&rft.spage=38295&rft.epage=38295&rft.pages=38295-38295&rft.artnum=38295&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/srep38295&rft_dat=%3Cproquest_pubme%3E1846368480%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1846368480&rft_id=info:pmid/27922110&rfr_iscdi=true |