Enhanced Performance and Mode of Action of a Novel Antibiofilm Hydrofiber® Wound Dressing
Biofilm development in wounds is now acknowledged to be a precursor to infection and a cause of delayed healing. A next-generation antibiofilm carboxymethylcellulose silver-containing wound dressing (NGAD) has been developed to disrupt and kill biofilm microorganisms. This in vitro study aimed to co...
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| Veröffentlicht in: | BioMed research international 2016-01, Vol.2016 (2016), p.1-14 |
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| creator | Metcalf, Daniel G. Short, Darryl Rowlands, Victoria J. Meredith, Kate Parsons, David Bowler, Philip G. |
| description | Biofilm development in wounds is now acknowledged to be a precursor to infection and a cause of delayed healing. A next-generation antibiofilm carboxymethylcellulose silver-containing wound dressing (NGAD) has been developed to disrupt and kill biofilm microorganisms. This in vitro study aimed to compare its effectiveness against various existing wound dressings and examine its mode of action. A number of biofilm models of increasing complexity were used to culture biofilms of wound-relevant pathogens, before exposure to test dressings. Confocal microscopy, staining, and imaging of biofilm constituents, total viable counting, and elemental analysis were conducted to assess dressing antibiofilm performance. Live/dead staining and viable counting of biofilms demonstrated that the NGAD was more effective at killing biofilm bacteria than two other standard silver dressings. Staining of biofilm polysaccharides showed that the NGAD was also more effective at reducing this protective biofilm component than standard silver dressings, and image analyses confirmed the superior biofilm killing and removal performance of the NGAD. The biofilm-disruptive and silver-enhancing modes of action of the NGAD were supported by significant differences (p |
| doi_str_mv | 10.1155/2016/7616471 |
| format | Article |
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A next-generation antibiofilm carboxymethylcellulose silver-containing wound dressing (NGAD) has been developed to disrupt and kill biofilm microorganisms. This in vitro study aimed to compare its effectiveness against various existing wound dressings and examine its mode of action. A number of biofilm models of increasing complexity were used to culture biofilms of wound-relevant pathogens, before exposure to test dressings. Confocal microscopy, staining, and imaging of biofilm constituents, total viable counting, and elemental analysis were conducted to assess dressing antibiofilm performance. Live/dead staining and viable counting of biofilms demonstrated that the NGAD was more effective at killing biofilm bacteria than two other standard silver dressings. Staining of biofilm polysaccharides showed that the NGAD was also more effective at reducing this protective biofilm component than standard silver dressings, and image analyses confirmed the superior biofilm killing and removal performance of the NGAD. The biofilm-disruptive and silver-enhancing modes of action of the NGAD were supported by significant differences (p<0.05) in biofilm elemental markers and silver donation. This in vitro study improves our understanding of how antibiofilm dressing technology can be effective against the challenge of biofilm.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2016/7616471</identifier><identifier>PMID: 27990437</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Antibiotics ; Antimicrobial agents ; Bacteria ; Bacteria - growth & development ; Bacterial Physiological Phenomena ; Bandages ; Biofilms ; Biofilms - growth & development ; Biomedical research ; Carboxymethylcellulose Sodium - chemistry ; Drug resistance ; Laboratories ; Microorganisms ; R&D ; Research & development ; Silver - chemistry ; Studies ; Wound Infection - microbiology ; Wound Infection - prevention & control</subject><ispartof>BioMed research international, 2016-01, Vol.2016 (2016), p.1-14</ispartof><rights>Copyright © 2016 David Parsons et al.</rights><rights>COPYRIGHT 2016 John Wiley & Sons, Inc.</rights><rights>Copyright © 2016 David Parsons et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2016 David Parsons et al. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-e2e111b7128aaa922204588899d10d6d1830d7888e75927ac8a9dd312c3e95d73</citedby><cites>FETCH-LOGICAL-c499t-e2e111b7128aaa922204588899d10d6d1830d7888e75927ac8a9dd312c3e95d73</cites><orcidid>0000-0003-2861-8948 ; 0000-0002-1762-5125 ; 0000-0002-4814-7533 ; 0000-0001-5130-2920 ; 0000-0003-2977-4862</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5136405/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5136405/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27990437$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Nithyanand, P.</contributor><creatorcontrib>Metcalf, Daniel G.</creatorcontrib><creatorcontrib>Short, Darryl</creatorcontrib><creatorcontrib>Rowlands, Victoria J.</creatorcontrib><creatorcontrib>Meredith, Kate</creatorcontrib><creatorcontrib>Parsons, David</creatorcontrib><creatorcontrib>Bowler, Philip G.</creatorcontrib><title>Enhanced Performance and Mode of Action of a Novel Antibiofilm Hydrofiber® Wound Dressing</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Biofilm development in wounds is now acknowledged to be a precursor to infection and a cause of delayed healing. A next-generation antibiofilm carboxymethylcellulose silver-containing wound dressing (NGAD) has been developed to disrupt and kill biofilm microorganisms. This in vitro study aimed to compare its effectiveness against various existing wound dressings and examine its mode of action. A number of biofilm models of increasing complexity were used to culture biofilms of wound-relevant pathogens, before exposure to test dressings. Confocal microscopy, staining, and imaging of biofilm constituents, total viable counting, and elemental analysis were conducted to assess dressing antibiofilm performance. Live/dead staining and viable counting of biofilms demonstrated that the NGAD was more effective at killing biofilm bacteria than two other standard silver dressings. Staining of biofilm polysaccharides showed that the NGAD was also more effective at reducing this protective biofilm component than standard silver dressings, and image analyses confirmed the superior biofilm killing and removal performance of the NGAD. The biofilm-disruptive and silver-enhancing modes of action of the NGAD were supported by significant differences (p<0.05) in biofilm elemental markers and silver donation. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Metcalf, Daniel G.</au><au>Short, Darryl</au><au>Rowlands, Victoria J.</au><au>Meredith, Kate</au><au>Parsons, David</au><au>Bowler, Philip G.</au><au>Nithyanand, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced Performance and Mode of Action of a Novel Antibiofilm Hydrofiber® Wound Dressing</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>2016</volume><issue>2016</issue><spage>1</spage><epage>14</epage><pages>1-14</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Biofilm development in wounds is now acknowledged to be a precursor to infection and a cause of delayed healing. A next-generation antibiofilm carboxymethylcellulose silver-containing wound dressing (NGAD) has been developed to disrupt and kill biofilm microorganisms. This in vitro study aimed to compare its effectiveness against various existing wound dressings and examine its mode of action. A number of biofilm models of increasing complexity were used to culture biofilms of wound-relevant pathogens, before exposure to test dressings. Confocal microscopy, staining, and imaging of biofilm constituents, total viable counting, and elemental analysis were conducted to assess dressing antibiofilm performance. Live/dead staining and viable counting of biofilms demonstrated that the NGAD was more effective at killing biofilm bacteria than two other standard silver dressings. Staining of biofilm polysaccharides showed that the NGAD was also more effective at reducing this protective biofilm component than standard silver dressings, and image analyses confirmed the superior biofilm killing and removal performance of the NGAD. The biofilm-disruptive and silver-enhancing modes of action of the NGAD were supported by significant differences (p<0.05) in biofilm elemental markers and silver donation. 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| subjects | Antibiotics Antimicrobial agents Bacteria Bacteria - growth & development Bacterial Physiological Phenomena Bandages Biofilms Biofilms - growth & development Biomedical research Carboxymethylcellulose Sodium - chemistry Drug resistance Laboratories Microorganisms R&D Research & development Silver - chemistry Studies Wound Infection - microbiology Wound Infection - prevention & control |
| title | Enhanced Performance and Mode of Action of a Novel Antibiofilm Hydrofiber® Wound Dressing |
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