A transferrin-target magnetic/fluorescent dual-mode probe significantly enhances the diagnosis of non-small cell lung cancer
To enhance the diagnosis of non-small cell lung cancer (NSCLC), we prepared a dual-modal probe Cy5.5-Tf-Gd-DTPA. Gd-DTPA and near-infrared (NIR) dyes were conjugated to holo-Transferrin (Tf) sequentially, the result of ICP-AES and UV showed 25 Gd ions and 1 Cy5.5 could be loaded per protein, respect...
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| creator | Cai, Jiali Gu, Bingxin Cao, Fengwen Liu, Shiyuan |
| description | To enhance the diagnosis of non-small cell lung cancer (NSCLC), we prepared a dual-modal probe Cy5.5-Tf-Gd-DTPA. Gd-DTPA and near-infrared (NIR) dyes were conjugated to holo-Transferrin (Tf) sequentially, the result of ICP-AES and UV showed 25 Gd ions and 1 Cy5.5 could be loaded per protein, respectively. The calculated longitudinal relaxivity R1 of Cy5.5-Tf-DTPA-Gd was 4.21 mM-1S-1 per Gd while that of Magnevist (Gd-DTPA) was only 4.02 mM-1S-1. Confocal laser scanning microscopy and immunohistochemical analyses revealed that the Cy5.5-Tf-DTPA-Gd was localized and accumulated in cytoplasmic vesicles; the cell toxicity assay showed no apparent toxicity. MR and NIR imaging of mice with subcutaneous H1299 xenografte tumors following intravenous injection of Cy5.5-Tf-DTPA-Gd revealed a strong positive contrast of the tumors, which caused a longer lasting enhancement of the MRI signal and fluorescence signal. Taken together, these studies indicate that Cy5.5-Tf-DTPA-Gd could be a good agent for MR/NIRF dual mode applications to detect both tumor in situ and its metastasis. |
| doi_str_mv | 10.18632/oncotarget.9482 |
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Gd-DTPA and near-infrared (NIR) dyes were conjugated to holo-Transferrin (Tf) sequentially, the result of ICP-AES and UV showed 25 Gd ions and 1 Cy5.5 could be loaded per protein, respectively. The calculated longitudinal relaxivity R1 of Cy5.5-Tf-DTPA-Gd was 4.21 mM-1S-1 per Gd while that of Magnevist (Gd-DTPA) was only 4.02 mM-1S-1. Confocal laser scanning microscopy and immunohistochemical analyses revealed that the Cy5.5-Tf-DTPA-Gd was localized and accumulated in cytoplasmic vesicles; the cell toxicity assay showed no apparent toxicity. MR and NIR imaging of mice with subcutaneous H1299 xenografte tumors following intravenous injection of Cy5.5-Tf-DTPA-Gd revealed a strong positive contrast of the tumors, which caused a longer lasting enhancement of the MRI signal and fluorescence signal. Taken together, these studies indicate that Cy5.5-Tf-DTPA-Gd could be a good agent for MR/NIRF dual mode applications to detect both tumor in situ and its metastasis.</description><identifier>ISSN: 1949-2553</identifier><identifier>EISSN: 1949-2553</identifier><identifier>DOI: 10.18632/oncotarget.9482</identifier><identifier>PMID: 27223075</identifier><language>eng</language><publisher>United States: Impact Journals LLC</publisher><subject>Animals ; Carbocyanines - chemistry ; Carcinoma, Non-Small-Cell Lung - diagnosis ; Cell Line, Tumor ; Cell Survival ; Fluorescence ; Fluorescent Dyes - chemistry ; Gadolinium - chemistry ; Gene Expression Regulation, Neoplastic ; Humans ; Ions ; Lung Neoplasms - diagnosis ; Magnetic Resonance Imaging ; Magnetics ; Male ; Mice ; Mice, Inbred BALB C ; Neoplasm Metastasis ; Neoplasm Transplantation ; Research Paper ; Spectroscopy, Near-Infrared ; Transferrin - chemistry ; Ultraviolet Rays</subject><ispartof>Oncotarget, 2016-06, Vol.7 (26), p.40047-40059</ispartof><rights>Copyright: © 2016 Cai et al. 2016</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-57037b1ed541a57fad35bfff0f0a1929e8d1fa9a6bcb9cb805815ddf8085ec0e3</citedby><cites>FETCH-LOGICAL-c424t-57037b1ed541a57fad35bfff0f0a1929e8d1fa9a6bcb9cb805815ddf8085ec0e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5129991/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5129991/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27223075$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cai, Jiali</creatorcontrib><creatorcontrib>Gu, Bingxin</creatorcontrib><creatorcontrib>Cao, Fengwen</creatorcontrib><creatorcontrib>Liu, Shiyuan</creatorcontrib><title>A transferrin-target magnetic/fluorescent dual-mode probe significantly enhances the diagnosis of non-small cell lung cancer</title><title>Oncotarget</title><addtitle>Oncotarget</addtitle><description>To enhance the diagnosis of non-small cell lung cancer (NSCLC), we prepared a dual-modal probe Cy5.5-Tf-Gd-DTPA. Gd-DTPA and near-infrared (NIR) dyes were conjugated to holo-Transferrin (Tf) sequentially, the result of ICP-AES and UV showed 25 Gd ions and 1 Cy5.5 could be loaded per protein, respectively. The calculated longitudinal relaxivity R1 of Cy5.5-Tf-DTPA-Gd was 4.21 mM-1S-1 per Gd while that of Magnevist (Gd-DTPA) was only 4.02 mM-1S-1. Confocal laser scanning microscopy and immunohistochemical analyses revealed that the Cy5.5-Tf-DTPA-Gd was localized and accumulated in cytoplasmic vesicles; the cell toxicity assay showed no apparent toxicity. MR and NIR imaging of mice with subcutaneous H1299 xenografte tumors following intravenous injection of Cy5.5-Tf-DTPA-Gd revealed a strong positive contrast of the tumors, which caused a longer lasting enhancement of the MRI signal and fluorescence signal. Taken together, these studies indicate that Cy5.5-Tf-DTPA-Gd could be a good agent for MR/NIRF dual mode applications to detect both tumor in situ and its metastasis.</description><subject>Animals</subject><subject>Carbocyanines - chemistry</subject><subject>Carcinoma, Non-Small-Cell Lung - diagnosis</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Gadolinium - chemistry</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Ions</subject><subject>Lung Neoplasms - diagnosis</subject><subject>Magnetic Resonance Imaging</subject><subject>Magnetics</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Neoplasm Metastasis</subject><subject>Neoplasm Transplantation</subject><subject>Research Paper</subject><subject>Spectroscopy, Near-Infrared</subject><subject>Transferrin - chemistry</subject><subject>Ultraviolet Rays</subject><issn>1949-2553</issn><issn>1949-2553</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc1rHSEUxSU0NCHJvqvisptJ1Bnf6KYQQr8gkE2yljt6nWeY0Vd1CoH-8Z3Xl6aJi6twz_1dD4eQD5xdcrVpxVWKNlXII9ZL3SlxRE657nQjpGzfvXqfkItSHtl6ZNcrod-TE9EL0bJenpLf17RmiMVjziE2BxydYYxYg73y05IyFouxUrfA1MzJId3lNCAtYYzBBwuxTk8U4xaixULrFqkLKyCVUGjyNKbYlBmmiVpcy7TEkdq9Np-TYw9TwYvn-4w8fP1yf_O9ub379uPm-raxnehqI3vW9gNHJzsOsvfgWjl475lnwLXQqBz3oGEz2EHbQTGpuHTOK6YkWobtGfl84O6WYUa3d5NhMrscZshPJkEwbzsxbM2YfhnJhdaar4BPz4Ccfi5YqplD2buBiGkphiux2SgtlFil7CC1OZWS0b-s4cz8zc38z83sc1tHPr7-3svAv5TaP018m4c</recordid><startdate>20160628</startdate><enddate>20160628</enddate><creator>Cai, Jiali</creator><creator>Gu, Bingxin</creator><creator>Cao, Fengwen</creator><creator>Liu, Shiyuan</creator><general>Impact Journals LLC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160628</creationdate><title>A transferrin-target magnetic/fluorescent dual-mode probe significantly enhances the diagnosis of non-small cell lung cancer</title><author>Cai, Jiali ; Gu, Bingxin ; Cao, Fengwen ; Liu, Shiyuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-57037b1ed541a57fad35bfff0f0a1929e8d1fa9a6bcb9cb805815ddf8085ec0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Carbocyanines - chemistry</topic><topic>Carcinoma, Non-Small-Cell Lung - diagnosis</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival</topic><topic>Fluorescence</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Gadolinium - chemistry</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Ions</topic><topic>Lung Neoplasms - diagnosis</topic><topic>Magnetic Resonance Imaging</topic><topic>Magnetics</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Neoplasm Metastasis</topic><topic>Neoplasm Transplantation</topic><topic>Research Paper</topic><topic>Spectroscopy, Near-Infrared</topic><topic>Transferrin - chemistry</topic><topic>Ultraviolet Rays</topic><toplevel>online_resources</toplevel><creatorcontrib>Cai, Jiali</creatorcontrib><creatorcontrib>Gu, Bingxin</creatorcontrib><creatorcontrib>Cao, Fengwen</creatorcontrib><creatorcontrib>Liu, Shiyuan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncotarget</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cai, Jiali</au><au>Gu, Bingxin</au><au>Cao, Fengwen</au><au>Liu, Shiyuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A transferrin-target magnetic/fluorescent dual-mode probe significantly enhances the diagnosis of non-small cell lung cancer</atitle><jtitle>Oncotarget</jtitle><addtitle>Oncotarget</addtitle><date>2016-06-28</date><risdate>2016</risdate><volume>7</volume><issue>26</issue><spage>40047</spage><epage>40059</epage><pages>40047-40059</pages><issn>1949-2553</issn><eissn>1949-2553</eissn><abstract>To enhance the diagnosis of non-small cell lung cancer (NSCLC), we prepared a dual-modal probe Cy5.5-Tf-Gd-DTPA. Gd-DTPA and near-infrared (NIR) dyes were conjugated to holo-Transferrin (Tf) sequentially, the result of ICP-AES and UV showed 25 Gd ions and 1 Cy5.5 could be loaded per protein, respectively. The calculated longitudinal relaxivity R1 of Cy5.5-Tf-DTPA-Gd was 4.21 mM-1S-1 per Gd while that of Magnevist (Gd-DTPA) was only 4.02 mM-1S-1. Confocal laser scanning microscopy and immunohistochemical analyses revealed that the Cy5.5-Tf-DTPA-Gd was localized and accumulated in cytoplasmic vesicles; the cell toxicity assay showed no apparent toxicity. MR and NIR imaging of mice with subcutaneous H1299 xenografte tumors following intravenous injection of Cy5.5-Tf-DTPA-Gd revealed a strong positive contrast of the tumors, which caused a longer lasting enhancement of the MRI signal and fluorescence signal. Taken together, these studies indicate that Cy5.5-Tf-DTPA-Gd could be a good agent for MR/NIRF dual mode applications to detect both tumor in situ and its metastasis.</abstract><cop>United States</cop><pub>Impact Journals LLC</pub><pmid>27223075</pmid><doi>10.18632/oncotarget.9482</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Carbocyanines - chemistry Carcinoma, Non-Small-Cell Lung - diagnosis Cell Line, Tumor Cell Survival Fluorescence Fluorescent Dyes - chemistry Gadolinium - chemistry Gene Expression Regulation, Neoplastic Humans Ions Lung Neoplasms - diagnosis Magnetic Resonance Imaging Magnetics Male Mice Mice, Inbred BALB C Neoplasm Metastasis Neoplasm Transplantation Research Paper Spectroscopy, Near-Infrared Transferrin - chemistry Ultraviolet Rays |
| title | A transferrin-target magnetic/fluorescent dual-mode probe significantly enhances the diagnosis of non-small cell lung cancer |
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