Permeabilization activated reduction in fluorescence: A novel method to measure kinetics of protein interactions with intracellular structures

Understanding kinetic information is fundamental in understanding biological function. Advanced imaging technologies have fostered the development of kinetic analyses in cells. We have developed Permeabilization Activated Reduction in Fluorescence (PARF) analysis for determination of apparent t1/2 a...

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Veröffentlicht in:	Cytoskeleton (Hoboken, N.J.) N.J.), 2016-06, Vol.73 (6), p.271-285
Hauptverfasser:	Singh, Pali P., Hawthorne, Jenci L., Davis, Christie A., Quintero, Omar A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Cytoskeletal Proteins - genetics Cytoskeletal Proteins - metabolism cytoskeleton Cytoskeleton - genetics Cytoskeleton - metabolism digitonin Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism HeLa Cells Humans Kinetics Microscopy, Fluorescence - methods protein-protein interactions quantitative fluorescence microscopy transient kinetic analysis
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