Assessment of serology and spirometry and the combination of both to complement microbiological isolation for earlier detection of Pseudomonas aeruginosa infection in children with cystic fibrosis
The aim of this study was to assess whether serology and spirometry and the combination of both can complement culture-based detection for earlier recognition of Pseudomonas aeruginosa infection in children with cystic fibrosis. A 4 year longitudinal prospective study that included 67 Slovenian chil...
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| Veröffentlicht in: | BMC pulmonary medicine 2016-11, Vol.16 (1), p.161-161, Article 161 |
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| creator | Kotnik Pirš, Ana Krivec, Uroš Simčič, Saša Seme, Katja |
| description | The aim of this study was to assess whether serology and spirometry and the combination of both can complement culture-based detection for earlier recognition of Pseudomonas aeruginosa infection in children with cystic fibrosis.
A 4 year longitudinal prospective study that included 67 Slovenian children with cystic fibrosis with a mean age of 10.5 years was conducted. Serology, spirometry and a scoring system combining serology and spirometry were assessed and compared. Infection was confirmed with isolation of Pseudomonas aeruginosa from respiratory samples.
There was a significantly positive correlation between serology and the combination of serology and spirometry and Pseudomonas aeruginosa isolation (P |
| doi_str_mv | 10.1186/s12890-016-0327-9 |
| format | Article |
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A 4 year longitudinal prospective study that included 67 Slovenian children with cystic fibrosis with a mean age of 10.5 years was conducted. Serology, spirometry and a scoring system combining serology and spirometry were assessed and compared. Infection was confirmed with isolation of Pseudomonas aeruginosa from respiratory samples.
There was a significantly positive correlation between serology and the combination of serology and spirometry and Pseudomonas aeruginosa isolation (P < 0.01 for both) and a significantly negative correlation between spirometry and Pseudomonas aeruginosa isolation (P < 0.05). An increase in serology for 1 ELISA unit increased the possibility of Pseudomonas aeruginosa isolation 1.6 times. A fall in FEV1% predicted for 10% increased the possibility of Pseudomonas aeruginosa isolation 9.8 times. Binary logistic regression analysis was used to determine the odds ratios and 95% confidence intervals for all three approaches. Serology had the highest specificity (0.80) and the combination of serology and spirometry the highest sensitivity (0.90). Both had a high negative predictive value (0.93 and 0.79 respectively).
Using serology and the combination of serology and lung function measurement can be beneficial for earlier detection of infection with Pseudomonas aeruginosa in children with cystic fibrosis when done simultaneously with standard culture-based detection from respiratory samples.</description><identifier>ISSN: 1471-2466</identifier><identifier>EISSN: 1471-2466</identifier><identifier>DOI: 10.1186/s12890-016-0327-9</identifier><identifier>PMID: 27884144</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Adolescent ; Antibodies, Bacterial ; Care and treatment ; Child ; Child, Preschool ; Cystic fibrosis ; Cystic Fibrosis - complications ; Cystic Fibrosis - microbiology ; Early Diagnosis ; Enzyme-linked immunosorbent assay ; Female ; Humans ; Infant ; Influence ; Logistic Models ; Longitudinal Studies ; Male ; Pediatric research ; Prospective Studies ; Pseudomonas aeruginosa - isolation & purification ; Pseudomonas aeruginosa infections ; Pseudomonas Infections - diagnosis ; Pulmonology ; ROC Curve ; Slovenia ; Spirometry ; Young Adult</subject><ispartof>BMC pulmonary medicine, 2016-11, Vol.16 (1), p.161-161, Article 161</ispartof><rights>COPYRIGHT 2016 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2016</rights><rights>The Author(s). 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-2e803f9aee221925507cb3fe2abff28105b6c61c3513cc106dfd94235dbfd40c3</citedby><cites>FETCH-LOGICAL-c494t-2e803f9aee221925507cb3fe2abff28105b6c61c3513cc106dfd94235dbfd40c3</cites><orcidid>0000-0002-1413-6844</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5123404/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5123404/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27884144$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kotnik Pirš, Ana</creatorcontrib><creatorcontrib>Krivec, Uroš</creatorcontrib><creatorcontrib>Simčič, Saša</creatorcontrib><creatorcontrib>Seme, Katja</creatorcontrib><title>Assessment of serology and spirometry and the combination of both to complement microbiological isolation for earlier detection of Pseudomonas aeruginosa infection in children with cystic fibrosis</title><title>BMC pulmonary medicine</title><addtitle>BMC Pulm Med</addtitle><description>The aim of this study was to assess whether serology and spirometry and the combination of both can complement culture-based detection for earlier recognition of Pseudomonas aeruginosa infection in children with cystic fibrosis.
A 4 year longitudinal prospective study that included 67 Slovenian children with cystic fibrosis with a mean age of 10.5 years was conducted. Serology, spirometry and a scoring system combining serology and spirometry were assessed and compared. Infection was confirmed with isolation of Pseudomonas aeruginosa from respiratory samples.
There was a significantly positive correlation between serology and the combination of serology and spirometry and Pseudomonas aeruginosa isolation (P < 0.01 for both) and a significantly negative correlation between spirometry and Pseudomonas aeruginosa isolation (P < 0.05). An increase in serology for 1 ELISA unit increased the possibility of Pseudomonas aeruginosa isolation 1.6 times. A fall in FEV1% predicted for 10% increased the possibility of Pseudomonas aeruginosa isolation 9.8 times. Binary logistic regression analysis was used to determine the odds ratios and 95% confidence intervals for all three approaches. Serology had the highest specificity (0.80) and the combination of serology and spirometry the highest sensitivity (0.90). Both had a high negative predictive value (0.93 and 0.79 respectively).
Using serology and the combination of serology and lung function measurement can be beneficial for earlier detection of infection with Pseudomonas aeruginosa in children with cystic fibrosis when done simultaneously with standard culture-based detection from respiratory samples.</description><subject>Adolescent</subject><subject>Antibodies, Bacterial</subject><subject>Care and treatment</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cystic fibrosis</subject><subject>Cystic Fibrosis - complications</subject><subject>Cystic Fibrosis - microbiology</subject><subject>Early Diagnosis</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Female</subject><subject>Humans</subject><subject>Infant</subject><subject>Influence</subject><subject>Logistic Models</subject><subject>Longitudinal Studies</subject><subject>Male</subject><subject>Pediatric research</subject><subject>Prospective Studies</subject><subject>Pseudomonas aeruginosa - isolation & purification</subject><subject>Pseudomonas aeruginosa infections</subject><subject>Pseudomonas Infections - diagnosis</subject><subject>Pulmonology</subject><subject>ROC Curve</subject><subject>Slovenia</subject><subject>Spirometry</subject><subject>Young Adult</subject><issn>1471-2466</issn><issn>1471-2466</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNptksluFDEQhlsIRELgAbggS1y4dPDW2wUpitikSHCAs-V2l2ccdbsGV3fQvB8PhpuZhAQhH7x99ZfL9RfFS8HPhWjrtyRk2_GSi7rkSjZl96g4FboRpdR1_fje-qR4RnTNuWjaSj0tTmTTtlpofVr8uiACognizNAzgoQjbvbMxoHRLiScYE6H7bwF5nDqQ7RzwLjiPc5bNuN6vBvhj8gUXMI-rCrB2ZEFwvHAe0wMbBoDJDbADO5W5SvBMuCE0RKzkJZNiEiWheiPTIjMbcM4JIjsZ8gp3Z7m4JgPfUIK9Lx44u1I8OI4nxXfP7z_dvmpvPry8fPlxVXpdKfnUkLLle8sgJSik1XFG9crD9L23stW8KqvXS2cqoRyTvB68EOnpaqG3g-aO3VWvDvo7pZ-gsHlepMdzS6Fyaa9QRvMw5sYtmaDN6YSUmmus8Cbo0DCHwvQbKZADsbRRsCFjGi15rJqVZfR1_-g17ikmMtbqUpJnVv_l9rYEUz-Mcx53SpqLnLzteSNWKnz_1B5DJDbhRF8yOcPAsQhIPeSKIG_q1Fws1rPHKxnsvXMaj2zPvjV_c-5i7j1mvoN_9rZ-w</recordid><startdate>20161125</startdate><enddate>20161125</enddate><creator>Kotnik Pirš, Ana</creator><creator>Krivec, Uroš</creator><creator>Simčič, Saša</creator><creator>Seme, Katja</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-1413-6844</orcidid></search><sort><creationdate>20161125</creationdate><title>Assessment of serology and spirometry and the combination of both to complement microbiological isolation for earlier detection of Pseudomonas aeruginosa infection in children with cystic fibrosis</title><author>Kotnik Pirš, Ana ; Krivec, Uroš ; Simčič, Saša ; Seme, Katja</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-2e803f9aee221925507cb3fe2abff28105b6c61c3513cc106dfd94235dbfd40c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adolescent</topic><topic>Antibodies, Bacterial</topic><topic>Care and treatment</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cystic fibrosis</topic><topic>Cystic Fibrosis - complications</topic><topic>Cystic Fibrosis - microbiology</topic><topic>Early Diagnosis</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Female</topic><topic>Humans</topic><topic>Infant</topic><topic>Influence</topic><topic>Logistic Models</topic><topic>Longitudinal Studies</topic><topic>Male</topic><topic>Pediatric research</topic><topic>Prospective Studies</topic><topic>Pseudomonas aeruginosa - isolation & purification</topic><topic>Pseudomonas aeruginosa infections</topic><topic>Pseudomonas Infections - diagnosis</topic><topic>Pulmonology</topic><topic>ROC Curve</topic><topic>Slovenia</topic><topic>Spirometry</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kotnik Pirš, Ana</creatorcontrib><creatorcontrib>Krivec, Uroš</creatorcontrib><creatorcontrib>Simčič, Saša</creatorcontrib><creatorcontrib>Seme, Katja</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BMC pulmonary medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kotnik Pirš, Ana</au><au>Krivec, Uroš</au><au>Simčič, Saša</au><au>Seme, Katja</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of serology and spirometry and the combination of both to complement microbiological isolation for earlier detection of Pseudomonas aeruginosa infection in children with cystic fibrosis</atitle><jtitle>BMC pulmonary medicine</jtitle><addtitle>BMC Pulm Med</addtitle><date>2016-11-25</date><risdate>2016</risdate><volume>16</volume><issue>1</issue><spage>161</spage><epage>161</epage><pages>161-161</pages><artnum>161</artnum><issn>1471-2466</issn><eissn>1471-2466</eissn><abstract>The aim of this study was to assess whether serology and spirometry and the combination of both can complement culture-based detection for earlier recognition of Pseudomonas aeruginosa infection in children with cystic fibrosis.
A 4 year longitudinal prospective study that included 67 Slovenian children with cystic fibrosis with a mean age of 10.5 years was conducted. Serology, spirometry and a scoring system combining serology and spirometry were assessed and compared. Infection was confirmed with isolation of Pseudomonas aeruginosa from respiratory samples.
There was a significantly positive correlation between serology and the combination of serology and spirometry and Pseudomonas aeruginosa isolation (P < 0.01 for both) and a significantly negative correlation between spirometry and Pseudomonas aeruginosa isolation (P < 0.05). An increase in serology for 1 ELISA unit increased the possibility of Pseudomonas aeruginosa isolation 1.6 times. A fall in FEV1% predicted for 10% increased the possibility of Pseudomonas aeruginosa isolation 9.8 times. Binary logistic regression analysis was used to determine the odds ratios and 95% confidence intervals for all three approaches. Serology had the highest specificity (0.80) and the combination of serology and spirometry the highest sensitivity (0.90). Both had a high negative predictive value (0.93 and 0.79 respectively).
Using serology and the combination of serology and lung function measurement can be beneficial for earlier detection of infection with Pseudomonas aeruginosa in children with cystic fibrosis when done simultaneously with standard culture-based detection from respiratory samples.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>27884144</pmid><doi>10.1186/s12890-016-0327-9</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-1413-6844</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; SpringerNature Journals; PubMed Central Open Access; Springer Nature OA Free Journals; PubMed Central |
| subjects | Adolescent Antibodies, Bacterial Care and treatment Child Child, Preschool Cystic fibrosis Cystic Fibrosis - complications Cystic Fibrosis - microbiology Early Diagnosis Enzyme-linked immunosorbent assay Female Humans Infant Influence Logistic Models Longitudinal Studies Male Pediatric research Prospective Studies Pseudomonas aeruginosa - isolation & purification Pseudomonas aeruginosa infections Pseudomonas Infections - diagnosis Pulmonology ROC Curve Slovenia Spirometry Young Adult |
| title | Assessment of serology and spirometry and the combination of both to complement microbiological isolation for earlier detection of Pseudomonas aeruginosa infection in children with cystic fibrosis |
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