Improved Separations of Proteins and Sugar Derivatives Using the Small-Scale Cross-Axis Coil Planet Centrifuge with Locular Multilayer Coiled Columns
Countercurrent chromatography (CCC) is liquid-liquid partition chromatography without using a solid support matrix. This technique requires further improvement of partition efficiency and shortening theseparation time. The locular multilayer coils modified with and without mixer glass beads were dev...
Gespeichert in:
| Veröffentlicht in: | Chromatography 2016-12, Vol.3 (4), p.29-29 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 29 |
|---|---|
| container_issue | 4 |
| container_start_page | 29 |
| container_title | Chromatography |
| container_volume | 3 |
| creator | Shinomiya, Kazufusa Umezawa, Motoki Seki, Manami Nitta, Jun Zaima, Kazumasa Harikai, Naoki Ito, Yoichiro |
| description | Countercurrent chromatography (CCC) is liquid-liquid partition chromatography without using a solid support matrix. This technique requires further improvement of partition efficiency and shortening theseparation time.
The locular multilayer coils modified with and without mixer glass beads were developed for the separation of proteins and 4-methylumbelliferyl (MU) sugar derivatives using the small-scale cross-axis coil planet centrifuge.
Proteins were well separated from each other and the separation was improved at a low flow rate of the mobile phase. On the other hand, 4-MU sugar derivatives were sufficiently resolved with short separation time at a highflow rate of the mobile phase under satisfactory stationary phase retention.
Effective separations were achieved using the locular multilayer coil for proteins with aqueous-aqueous polymer phase systems and for 4-MU sugar derivatives with organic-aqueous two-phase solvent systems by inserting a glass bead into each locule. |
| doi_str_mv | 10.3390/separations3040029 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5119458</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4301345471</sourcerecordid><originalsourceid>FETCH-LOGICAL-c463t-109a5391c4cf43d9aaf215de7a4936e0615d4da037d8ffeff3b357f15a0546243</originalsourceid><addsrcrecordid>eNqNks9u1DAQxi0EolXpC3BAlrhwCfhvEl-QqgCl0iIqLT1bbjLedeXEix0v9EF43zq0lAIXTh5rfvPNN6NB6DklrzlX5E2CnYlmdmFKnAhCmHqEDhlTTdU2XD1-EB-g45SuCCG04XVN2VN0wJpWUUmaQ_TjbNzFsIcBr38L4mDxeQwzuBKbqeTyxkT8DqLbF2IPCV8kN23wvAW8Ho331bo3HnAXQ0rVyXeXcBecx-feTDDjDqY5Ops3gL-5eYtXoc--CH7KfnbeXEP8iRcTXfB5nNIz9MQan-D47j1CFx_ef-k-VqvPp2fdyarqRc3nihJlJFe0F70VfFDGWEblAI0RitdA6vIRgyG8GVprwVp-yWVjqTREipoJfoTe3uru8uUIQ7_4NF7vohtNvNbBOP1nZnJbvQl7LSlVQrZF4NWdQAxfM6RZjy714Je5Q06atm3Ze80Z_w9UCC45JYvqy7_Qq5DjVDZRqNKVtnUrC8VuqX7ZegR775sSvdyI_vdGStGLhxPfl_y6CH4D4I29TA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1858318685</pqid></control><display><type>article</type><title>Improved Separations of Proteins and Sugar Derivatives Using the Small-Scale Cross-Axis Coil Planet Centrifuge with Locular Multilayer Coiled Columns</title><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Shinomiya, Kazufusa ; Umezawa, Motoki ; Seki, Manami ; Nitta, Jun ; Zaima, Kazumasa ; Harikai, Naoki ; Ito, Yoichiro</creator><creatorcontrib>Shinomiya, Kazufusa ; Umezawa, Motoki ; Seki, Manami ; Nitta, Jun ; Zaima, Kazumasa ; Harikai, Naoki ; Ito, Yoichiro</creatorcontrib><description>Countercurrent chromatography (CCC) is liquid-liquid partition chromatography without using a solid support matrix. This technique requires further improvement of partition efficiency and shortening theseparation time.
The locular multilayer coils modified with and without mixer glass beads were developed for the separation of proteins and 4-methylumbelliferyl (MU) sugar derivatives using the small-scale cross-axis coil planet centrifuge.
Proteins were well separated from each other and the separation was improved at a low flow rate of the mobile phase. On the other hand, 4-MU sugar derivatives were sufficiently resolved with short separation time at a highflow rate of the mobile phase under satisfactory stationary phase retention.
Effective separations were achieved using the locular multilayer coil for proteins with aqueous-aqueous polymer phase systems and for 4-MU sugar derivatives with organic-aqueous two-phase solvent systems by inserting a glass bead into each locule.</description><identifier>ISSN: 2297-8739</identifier><identifier>EISSN: 2297-8739</identifier><identifier>EISSN: 2227-9075</identifier><identifier>DOI: 10.3390/separations3040029</identifier><identifier>PMID: 27891507</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Beads ; Chromatography ; Coiling ; Derivatives ; Multilayers ; Proteins ; Separation ; Sugars</subject><ispartof>Chromatography, 2016-12, Vol.3 (4), p.29-29</ispartof><rights>Copyright MDPI AG 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-109a5391c4cf43d9aaf215de7a4936e0615d4da037d8ffeff3b357f15a0546243</citedby><cites>FETCH-LOGICAL-c463t-109a5391c4cf43d9aaf215de7a4936e0615d4da037d8ffeff3b357f15a0546243</cites><orcidid>0000-0001-7897-6546</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,860,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27891507$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shinomiya, Kazufusa</creatorcontrib><creatorcontrib>Umezawa, Motoki</creatorcontrib><creatorcontrib>Seki, Manami</creatorcontrib><creatorcontrib>Nitta, Jun</creatorcontrib><creatorcontrib>Zaima, Kazumasa</creatorcontrib><creatorcontrib>Harikai, Naoki</creatorcontrib><creatorcontrib>Ito, Yoichiro</creatorcontrib><title>Improved Separations of Proteins and Sugar Derivatives Using the Small-Scale Cross-Axis Coil Planet Centrifuge with Locular Multilayer Coiled Columns</title><title>Chromatography</title><addtitle>Separations</addtitle><description>Countercurrent chromatography (CCC) is liquid-liquid partition chromatography without using a solid support matrix. This technique requires further improvement of partition efficiency and shortening theseparation time.
The locular multilayer coils modified with and without mixer glass beads were developed for the separation of proteins and 4-methylumbelliferyl (MU) sugar derivatives using the small-scale cross-axis coil planet centrifuge.
Proteins were well separated from each other and the separation was improved at a low flow rate of the mobile phase. On the other hand, 4-MU sugar derivatives were sufficiently resolved with short separation time at a highflow rate of the mobile phase under satisfactory stationary phase retention.
Effective separations were achieved using the locular multilayer coil for proteins with aqueous-aqueous polymer phase systems and for 4-MU sugar derivatives with organic-aqueous two-phase solvent systems by inserting a glass bead into each locule.</description><subject>Beads</subject><subject>Chromatography</subject><subject>Coiling</subject><subject>Derivatives</subject><subject>Multilayers</subject><subject>Proteins</subject><subject>Separation</subject><subject>Sugars</subject><issn>2297-8739</issn><issn>2297-8739</issn><issn>2227-9075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNqNks9u1DAQxi0EolXpC3BAlrhwCfhvEl-QqgCl0iIqLT1bbjLedeXEix0v9EF43zq0lAIXTh5rfvPNN6NB6DklrzlX5E2CnYlmdmFKnAhCmHqEDhlTTdU2XD1-EB-g45SuCCG04XVN2VN0wJpWUUmaQ_TjbNzFsIcBr38L4mDxeQwzuBKbqeTyxkT8DqLbF2IPCV8kN23wvAW8Ho331bo3HnAXQ0rVyXeXcBecx-feTDDjDqY5Ops3gL-5eYtXoc--CH7KfnbeXEP8iRcTXfB5nNIz9MQan-D47j1CFx_ef-k-VqvPp2fdyarqRc3nihJlJFe0F70VfFDGWEblAI0RitdA6vIRgyG8GVprwVp-yWVjqTREipoJfoTe3uru8uUIQ7_4NF7vohtNvNbBOP1nZnJbvQl7LSlVQrZF4NWdQAxfM6RZjy714Je5Q06atm3Ze80Z_w9UCC45JYvqy7_Qq5DjVDZRqNKVtnUrC8VuqX7ZegR775sSvdyI_vdGStGLhxPfl_y6CH4D4I29TA</recordid><startdate>20161201</startdate><enddate>20161201</enddate><creator>Shinomiya, Kazufusa</creator><creator>Umezawa, Motoki</creator><creator>Seki, Manami</creator><creator>Nitta, Jun</creator><creator>Zaima, Kazumasa</creator><creator>Harikai, Naoki</creator><creator>Ito, Yoichiro</creator><general>MDPI AG</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>L7M</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7897-6546</orcidid></search><sort><creationdate>20161201</creationdate><title>Improved Separations of Proteins and Sugar Derivatives Using the Small-Scale Cross-Axis Coil Planet Centrifuge with Locular Multilayer Coiled Columns</title><author>Shinomiya, Kazufusa ; Umezawa, Motoki ; Seki, Manami ; Nitta, Jun ; Zaima, Kazumasa ; Harikai, Naoki ; Ito, Yoichiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-109a5391c4cf43d9aaf215de7a4936e0615d4da037d8ffeff3b357f15a0546243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Beads</topic><topic>Chromatography</topic><topic>Coiling</topic><topic>Derivatives</topic><topic>Multilayers</topic><topic>Proteins</topic><topic>Separation</topic><topic>Sugars</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shinomiya, Kazufusa</creatorcontrib><creatorcontrib>Umezawa, Motoki</creatorcontrib><creatorcontrib>Seki, Manami</creatorcontrib><creatorcontrib>Nitta, Jun</creatorcontrib><creatorcontrib>Zaima, Kazumasa</creatorcontrib><creatorcontrib>Harikai, Naoki</creatorcontrib><creatorcontrib>Ito, Yoichiro</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>Materials Science Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shinomiya, Kazufusa</au><au>Umezawa, Motoki</au><au>Seki, Manami</au><au>Nitta, Jun</au><au>Zaima, Kazumasa</au><au>Harikai, Naoki</au><au>Ito, Yoichiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved Separations of Proteins and Sugar Derivatives Using the Small-Scale Cross-Axis Coil Planet Centrifuge with Locular Multilayer Coiled Columns</atitle><jtitle>Chromatography</jtitle><addtitle>Separations</addtitle><date>2016-12-01</date><risdate>2016</risdate><volume>3</volume><issue>4</issue><spage>29</spage><epage>29</epage><pages>29-29</pages><issn>2297-8739</issn><eissn>2297-8739</eissn><eissn>2227-9075</eissn><abstract>Countercurrent chromatography (CCC) is liquid-liquid partition chromatography without using a solid support matrix. This technique requires further improvement of partition efficiency and shortening theseparation time.
The locular multilayer coils modified with and without mixer glass beads were developed for the separation of proteins and 4-methylumbelliferyl (MU) sugar derivatives using the small-scale cross-axis coil planet centrifuge.
Proteins were well separated from each other and the separation was improved at a low flow rate of the mobile phase. On the other hand, 4-MU sugar derivatives were sufficiently resolved with short separation time at a highflow rate of the mobile phase under satisfactory stationary phase retention.
Effective separations were achieved using the locular multilayer coil for proteins with aqueous-aqueous polymer phase systems and for 4-MU sugar derivatives with organic-aqueous two-phase solvent systems by inserting a glass bead into each locule.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>27891507</pmid><doi>10.3390/separations3040029</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-7897-6546</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2297-8739 |
| ispartof | Chromatography, 2016-12, Vol.3 (4), p.29-29 |
| issn | 2297-8739 2297-8739 2227-9075 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5119458 |
| source | MDPI - Multidisciplinary Digital Publishing Institute; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Beads Chromatography Coiling Derivatives Multilayers Proteins Separation Sugars |
| title | Improved Separations of Proteins and Sugar Derivatives Using the Small-Scale Cross-Axis Coil Planet Centrifuge with Locular Multilayer Coiled Columns |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T14%3A42%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Improved%20Separations%20of%20Proteins%20and%20Sugar%20Derivatives%20Using%20the%20Small-Scale%20Cross-Axis%20Coil%20Planet%20Centrifuge%20with%20Locular%20Multilayer%20Coiled%20Columns&rft.jtitle=Chromatography&rft.au=Shinomiya,%20Kazufusa&rft.date=2016-12-01&rft.volume=3&rft.issue=4&rft.spage=29&rft.epage=29&rft.pages=29-29&rft.issn=2297-8739&rft.eissn=2297-8739&rft_id=info:doi/10.3390/separations3040029&rft_dat=%3Cproquest_pubme%3E4301345471%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1858318685&rft_id=info:pmid/27891507&rfr_iscdi=true |