The alteration of mRNA expression of SOD and GPX genes, and proteins in tomato (Lycopersicon esculentum Mill) under stress of NaCl and/or ZnO nanoparticles
Five cultivars of tomato having different levels of salt stress tolerance were exposed to different treatments of NaCl (0, 3 and 6gL−1) and ZnO-NPs (0, 15 and 30mgL−1). Treatments with NaCl at both 3 and 6gL−1 suppressed the mRNA levels of superoxide dismutase (SOD) and glutathione peroxidase (GPX)...
Gespeichert in:
| Veröffentlicht in: | Saudi journal of biological sciences 2016-11, Vol.23 (6), p.773-781 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 781 |
|---|---|
| container_issue | 6 |
| container_start_page | 773 |
| container_title | Saudi journal of biological sciences |
| container_volume | 23 |
| creator | Alharby, Hesham F. Metwali, Ehab M.R. Fuller, Michael P. Aldhebiani, Amal Y. |
| description | Five cultivars of tomato having different levels of salt stress tolerance were exposed to different treatments of NaCl (0, 3 and 6gL−1) and ZnO-NPs (0, 15 and 30mgL−1). Treatments with NaCl at both 3 and 6gL−1 suppressed the mRNA levels of superoxide dismutase (SOD) and glutathione peroxidase (GPX) genes in all cultivars while plants treated with ZnO-NPs in the presence of NaCl, showed increments in the mRNA expression levels. This indicated that ZnO-NPs had a positive response on plant metabolism under salt stress. Superior expression levels of mRNA were observed in the salt tolerant cultivars, Sandpoint and Edkawy while the lowest level was detected in the salt sensitive cultivar, Anna Aasa. SDS–PAGE showed clear differences in patterns of protein expression among the cultivars. A negative protein marker for salt sensitivity and ZnO-NPs was detected in cv. Anna Aasa at a molecular weight of 19.162kDa, while the tolerant cultivar Edkawy had two positive markers at molecular weights of 74.991 and 79.735kDa. |
| doi_str_mv | 10.1016/j.sjbs.2016.04.012 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5109485</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1319562X16300249</els_id><sourcerecordid>2000448936</sourcerecordid><originalsourceid>FETCH-LOGICAL-c510t-ff045072d60aa4ea374f94108e48e7053ed1516028c79b661d19a1539aa46c773</originalsourceid><addsrcrecordid>eNqFktFu0zAUhiMEYqXwAkggXw6JZraT2I40IU0FBlJZEQxp4sZynZPNVWIH25nYs_CyOLRUcANXts_5zn-O7T_LnhKcE0zYyTYP203IadrnuMwxofeyGaWkWHCC2f1sRgpSLypGr46yRyFsMWaiEORhdkS54LTibJb9uLwBpLoIXkXjLHIt6j9dnCH4PngIYR_6vH6NlG3Q-ccrdA0Wwstfx8G7CMYGZCyKrlfRoePVnXYD-GB0KoWgxw5sHHv0wXTdCzTaBjwKcdKehC_UspukTpxHX-0aWWXdoHw0uoPwOHvQqi7Ak_06z768fXO5fLdYrc_fL89WC10RHBdti8sKc9owrFQJquBlW5cECygFcFwV0JCKMEyF5vWGMdKQWpGqqBPNNOfFPHu10x3GTQ-NTgN71cnBm175O-mUkX9nrLmR1-5WpvZ1KaokcLwX8O7bCCHK3gQNXacsuDFIijEuS1EX7L8oESWt6lokeJ7RHaq9C8FDe5iIYDkZQG7lZAA5GUDiUiYDpKLnf97lUPL7xxPwbAdAikOrDgQnnAiS8qf7fHrxWwNeBm3AamiMBx1l48y_-v8EpmzM1w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1842599889</pqid></control><display><type>article</type><title>The alteration of mRNA expression of SOD and GPX genes, and proteins in tomato (Lycopersicon esculentum Mill) under stress of NaCl and/or ZnO nanoparticles</title><source>Elsevier ScienceDirect Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Alharby, Hesham F. ; Metwali, Ehab M.R. ; Fuller, Michael P. ; Aldhebiani, Amal Y.</creator><creatorcontrib>Alharby, Hesham F. ; Metwali, Ehab M.R. ; Fuller, Michael P. ; Aldhebiani, Amal Y.</creatorcontrib><description>Five cultivars of tomato having different levels of salt stress tolerance were exposed to different treatments of NaCl (0, 3 and 6gL−1) and ZnO-NPs (0, 15 and 30mgL−1). Treatments with NaCl at both 3 and 6gL−1 suppressed the mRNA levels of superoxide dismutase (SOD) and glutathione peroxidase (GPX) genes in all cultivars while plants treated with ZnO-NPs in the presence of NaCl, showed increments in the mRNA expression levels. This indicated that ZnO-NPs had a positive response on plant metabolism under salt stress. Superior expression levels of mRNA were observed in the salt tolerant cultivars, Sandpoint and Edkawy while the lowest level was detected in the salt sensitive cultivar, Anna Aasa. SDS–PAGE showed clear differences in patterns of protein expression among the cultivars. A negative protein marker for salt sensitivity and ZnO-NPs was detected in cv. Anna Aasa at a molecular weight of 19.162kDa, while the tolerant cultivar Edkawy had two positive markers at molecular weights of 74.991 and 79.735kDa.</description><identifier>ISSN: 1319-562X</identifier><identifier>EISSN: 2213-7106</identifier><identifier>DOI: 10.1016/j.sjbs.2016.04.012</identifier><identifier>PMID: 27872576</identifier><language>eng</language><publisher>Riyadh, Saudi Arabia: Elsevier B.V</publisher><subject>cultivars ; Gene expression ; genes ; glutathione peroxidase ; messenger RNA ; molecular weight ; Nanoparticles ; Original ; polyacrylamide gel electrophoresis ; Polymorphism ; protein synthesis ; proteins ; Real-time PCR ; Salt stress ; salt tolerance ; sodium chloride ; Solanum lycopersicum var. lycopersicum ; stress tolerance ; superoxide dismutase ; Tomato ; tomatoes ; zinc oxide</subject><ispartof>Saudi journal of biological sciences, 2016-11, Vol.23 (6), p.773-781</ispartof><rights>2016 The Authors</rights><rights>2016 The Authors 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c510t-ff045072d60aa4ea374f94108e48e7053ed1516028c79b661d19a1539aa46c773</citedby><cites>FETCH-LOGICAL-c510t-ff045072d60aa4ea374f94108e48e7053ed1516028c79b661d19a1539aa46c773</cites><orcidid>0000-0003-3461-9728</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5109485/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.sjbs.2016.04.012$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,3537,27905,27906,45976,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27872576$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alharby, Hesham F.</creatorcontrib><creatorcontrib>Metwali, Ehab M.R.</creatorcontrib><creatorcontrib>Fuller, Michael P.</creatorcontrib><creatorcontrib>Aldhebiani, Amal Y.</creatorcontrib><title>The alteration of mRNA expression of SOD and GPX genes, and proteins in tomato (Lycopersicon esculentum Mill) under stress of NaCl and/or ZnO nanoparticles</title><title>Saudi journal of biological sciences</title><addtitle>Saudi J Biol Sci</addtitle><description>Five cultivars of tomato having different levels of salt stress tolerance were exposed to different treatments of NaCl (0, 3 and 6gL−1) and ZnO-NPs (0, 15 and 30mgL−1). Treatments with NaCl at both 3 and 6gL−1 suppressed the mRNA levels of superoxide dismutase (SOD) and glutathione peroxidase (GPX) genes in all cultivars while plants treated with ZnO-NPs in the presence of NaCl, showed increments in the mRNA expression levels. This indicated that ZnO-NPs had a positive response on plant metabolism under salt stress. Superior expression levels of mRNA were observed in the salt tolerant cultivars, Sandpoint and Edkawy while the lowest level was detected in the salt sensitive cultivar, Anna Aasa. SDS–PAGE showed clear differences in patterns of protein expression among the cultivars. A negative protein marker for salt sensitivity and ZnO-NPs was detected in cv. Anna Aasa at a molecular weight of 19.162kDa, while the tolerant cultivar Edkawy had two positive markers at molecular weights of 74.991 and 79.735kDa.</description><subject>cultivars</subject><subject>Gene expression</subject><subject>genes</subject><subject>glutathione peroxidase</subject><subject>messenger RNA</subject><subject>molecular weight</subject><subject>Nanoparticles</subject><subject>Original</subject><subject>polyacrylamide gel electrophoresis</subject><subject>Polymorphism</subject><subject>protein synthesis</subject><subject>proteins</subject><subject>Real-time PCR</subject><subject>Salt stress</subject><subject>salt tolerance</subject><subject>sodium chloride</subject><subject>Solanum lycopersicum var. lycopersicum</subject><subject>stress tolerance</subject><subject>superoxide dismutase</subject><subject>Tomato</subject><subject>tomatoes</subject><subject>zinc oxide</subject><issn>1319-562X</issn><issn>2213-7106</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqFktFu0zAUhiMEYqXwAkggXw6JZraT2I40IU0FBlJZEQxp4sZynZPNVWIH25nYs_CyOLRUcANXts_5zn-O7T_LnhKcE0zYyTYP203IadrnuMwxofeyGaWkWHCC2f1sRgpSLypGr46yRyFsMWaiEORhdkS54LTibJb9uLwBpLoIXkXjLHIt6j9dnCH4PngIYR_6vH6NlG3Q-ccrdA0Wwstfx8G7CMYGZCyKrlfRoePVnXYD-GB0KoWgxw5sHHv0wXTdCzTaBjwKcdKehC_UspukTpxHX-0aWWXdoHw0uoPwOHvQqi7Ak_06z768fXO5fLdYrc_fL89WC10RHBdti8sKc9owrFQJquBlW5cECygFcFwV0JCKMEyF5vWGMdKQWpGqqBPNNOfFPHu10x3GTQ-NTgN71cnBm175O-mUkX9nrLmR1-5WpvZ1KaokcLwX8O7bCCHK3gQNXacsuDFIijEuS1EX7L8oESWt6lokeJ7RHaq9C8FDe5iIYDkZQG7lZAA5GUDiUiYDpKLnf97lUPL7xxPwbAdAikOrDgQnnAiS8qf7fHrxWwNeBm3AamiMBx1l48y_-v8EpmzM1w</recordid><startdate>20161101</startdate><enddate>20161101</enddate><creator>Alharby, Hesham F.</creator><creator>Metwali, Ehab M.R.</creator><creator>Fuller, Michael P.</creator><creator>Aldhebiani, Amal Y.</creator><general>Elsevier B.V</general><general>Saudi Biological Society</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>ADJCN</scope><scope>AHFXO</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3461-9728</orcidid></search><sort><creationdate>20161101</creationdate><title>The alteration of mRNA expression of SOD and GPX genes, and proteins in tomato (Lycopersicon esculentum Mill) under stress of NaCl and/or ZnO nanoparticles</title><author>Alharby, Hesham F. ; Metwali, Ehab M.R. ; Fuller, Michael P. ; Aldhebiani, Amal Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c510t-ff045072d60aa4ea374f94108e48e7053ed1516028c79b661d19a1539aa46c773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>cultivars</topic><topic>Gene expression</topic><topic>genes</topic><topic>glutathione peroxidase</topic><topic>messenger RNA</topic><topic>molecular weight</topic><topic>Nanoparticles</topic><topic>Original</topic><topic>polyacrylamide gel electrophoresis</topic><topic>Polymorphism</topic><topic>protein synthesis</topic><topic>proteins</topic><topic>Real-time PCR</topic><topic>Salt stress</topic><topic>salt tolerance</topic><topic>sodium chloride</topic><topic>Solanum lycopersicum var. lycopersicum</topic><topic>stress tolerance</topic><topic>superoxide dismutase</topic><topic>Tomato</topic><topic>tomatoes</topic><topic>zinc oxide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alharby, Hesham F.</creatorcontrib><creatorcontrib>Metwali, Ehab M.R.</creatorcontrib><creatorcontrib>Fuller, Michael P.</creatorcontrib><creatorcontrib>Aldhebiani, Amal Y.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Saudi journal of biological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alharby, Hesham F.</au><au>Metwali, Ehab M.R.</au><au>Fuller, Michael P.</au><au>Aldhebiani, Amal Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The alteration of mRNA expression of SOD and GPX genes, and proteins in tomato (Lycopersicon esculentum Mill) under stress of NaCl and/or ZnO nanoparticles</atitle><jtitle>Saudi journal of biological sciences</jtitle><addtitle>Saudi J Biol Sci</addtitle><date>2016-11-01</date><risdate>2016</risdate><volume>23</volume><issue>6</issue><spage>773</spage><epage>781</epage><pages>773-781</pages><issn>1319-562X</issn><eissn>2213-7106</eissn><abstract>Five cultivars of tomato having different levels of salt stress tolerance were exposed to different treatments of NaCl (0, 3 and 6gL−1) and ZnO-NPs (0, 15 and 30mgL−1). Treatments with NaCl at both 3 and 6gL−1 suppressed the mRNA levels of superoxide dismutase (SOD) and glutathione peroxidase (GPX) genes in all cultivars while plants treated with ZnO-NPs in the presence of NaCl, showed increments in the mRNA expression levels. This indicated that ZnO-NPs had a positive response on plant metabolism under salt stress. Superior expression levels of mRNA were observed in the salt tolerant cultivars, Sandpoint and Edkawy while the lowest level was detected in the salt sensitive cultivar, Anna Aasa. SDS–PAGE showed clear differences in patterns of protein expression among the cultivars. A negative protein marker for salt sensitivity and ZnO-NPs was detected in cv. Anna Aasa at a molecular weight of 19.162kDa, while the tolerant cultivar Edkawy had two positive markers at molecular weights of 74.991 and 79.735kDa.</abstract><cop>Riyadh, Saudi Arabia</cop><pub>Elsevier B.V</pub><pmid>27872576</pmid><doi>10.1016/j.sjbs.2016.04.012</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-3461-9728</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1319-562X |
| ispartof | Saudi journal of biological sciences, 2016-11, Vol.23 (6), p.773-781 |
| issn | 1319-562X 2213-7106 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5109485 |
| source | Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | cultivars Gene expression genes glutathione peroxidase messenger RNA molecular weight Nanoparticles Original polyacrylamide gel electrophoresis Polymorphism protein synthesis proteins Real-time PCR Salt stress salt tolerance sodium chloride Solanum lycopersicum var. lycopersicum stress tolerance superoxide dismutase Tomato tomatoes zinc oxide |
| title | The alteration of mRNA expression of SOD and GPX genes, and proteins in tomato (Lycopersicon esculentum Mill) under stress of NaCl and/or ZnO nanoparticles |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T09%3A56%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20alteration%20of%20mRNA%20expression%20of%20SOD%20and%20GPX%20genes,%20and%20proteins%20in%20tomato%20(Lycopersicon%20esculentum%20Mill)%20under%20stress%20of%20NaCl%20and/or%20ZnO%20nanoparticles&rft.jtitle=Saudi%20journal%20of%20biological%20sciences&rft.au=Alharby,%20Hesham%20F.&rft.date=2016-11-01&rft.volume=23&rft.issue=6&rft.spage=773&rft.epage=781&rft.pages=773-781&rft.issn=1319-562X&rft.eissn=2213-7106&rft_id=info:doi/10.1016/j.sjbs.2016.04.012&rft_dat=%3Cproquest_pubme%3E2000448936%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1842599889&rft_id=info:pmid/27872576&rft_els_id=S1319562X16300249&rfr_iscdi=true |