Serum availability affects expression of common house-keeping genes in colon adenocarcinoma cell lines: implications for quantitative real-time PCR studies

Careful selection of housekeeping genes (HKG) is prerequisite to yield sound qPCR results. HKG expression varies in response to hypoxia but the effect of manipulations of serum availability, a common experimental procedure, remains unknown. Also, no data on HKG expression stability across colon aden...

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Veröffentlicht in:	Cytotechnology (Dordrecht) 2016-12, Vol.68 (6), p.2503-2517
Hauptverfasser:	Krzystek-Korpacka, Malgorzata, Hotowy, Katarzyna, Czapinska, Elzbieta, Podkowik, Magdalena, Bania, Jacek, Gamian, Andrzej, Bednarz-Misa, Iwona
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenocarcinoma Automation Biochemistry Biomedicine Biotechnology Cancer Cell culture Chemistry Chemistry and Materials Science Colon Dehydrogenases Enzymes Genes Glyceraldehyde-3-phosphate dehydrogenase Growth conditions Hypoxia Kinases Original Original Article p53 Protein Polymerase chain reaction Proteins Signal transduction Tumor cell lines
Online-Zugang:	Volltext
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