In vitro cytotoxic and genotoxic evaluation of peptides used in nuclear medicine (DOTATATE and Ubiquicidin29-41) in CHO-K1 cells
Micronucleus (MN) assay constitutes a valuable surrogate to the chromosome aberration technique for in vitro testing of the genotoxicity of substances. As test substances, two peptidic compounds (DOTATATE and Ubiquicidin 29-41 ) used in nuclear medicine, were tested for in vitro cytotoxicity and gen...
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| Veröffentlicht in: | Cytotechnology (Dordrecht) 2016-12, Vol.68 (6), p.2301-2310 |
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| creator | Ocampo, Ivette Zegarra de Queiroz Souza Passos, Priscila Ramirez de Carvalho, Luma Lira da Cruz, Camila Ayala Esteves-Pedro, Natália Mencacci Medeiros da Silva, Fabiana Higa, Olga Zazuco Dias, Luiz Alberto Pereira Okazaki, Kayo Vieira, Daniel Perez |
| description | Micronucleus (MN) assay constitutes a valuable surrogate to the chromosome aberration technique for in vitro testing of the genotoxicity of substances. As test substances, two peptidic compounds (DOTATATE and Ubiquicidin
29-41
) used in nuclear medicine, were tested for in vitro cytotoxicity and genotoxicity in CHO-K1 cells. None of the compounds showed detectable cytotoxicity (0.5–7.3 ng/mL for DOTATATE and 0.3–4.5 ng/mL for UBI
29-41
), genotoxicity (0.72, 7.2 and 72.0 ng/ml for DOTATATE and 0.45, 4.5 and 45.0 ng/mL for UBI
29-41
) or cell cycle changes as compared to untreated controls at the concentrations tested. Statistical analysis showed good concordance between two independent analysts. The results corroborate the notion of the safety of the compounds and present improvements of the in vitro MN assay when performed in a pre-clinical trial context that increase the throughput of small-to-medium testing facilities as an alternative to high content screening systems. |
| doi_str_mv | 10.1007/s10616-016-0024-9 |
| format | Article |
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29-41
) used in nuclear medicine, were tested for in vitro cytotoxicity and genotoxicity in CHO-K1 cells. None of the compounds showed detectable cytotoxicity (0.5–7.3 ng/mL for DOTATATE and 0.3–4.5 ng/mL for UBI
29-41
), genotoxicity (0.72, 7.2 and 72.0 ng/ml for DOTATATE and 0.45, 4.5 and 45.0 ng/mL for UBI
29-41
) or cell cycle changes as compared to untreated controls at the concentrations tested. Statistical analysis showed good concordance between two independent analysts. The results corroborate the notion of the safety of the compounds and present improvements of the in vitro MN assay when performed in a pre-clinical trial context that increase the throughput of small-to-medium testing facilities as an alternative to high content screening systems.</description><identifier>ISSN: 0920-9069</identifier><identifier>EISSN: 1573-0778</identifier><identifier>DOI: 10.1007/s10616-016-0024-9</identifier><identifier>PMID: 27686814</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Automation ; Biochemistry ; Biomedicine ; Biotechnology ; Cell cycle ; Cell division ; Chemistry ; Chemistry and Materials Science ; Chromosome aberrations ; Chromosomes ; Cytotoxicity ; Drug dosages ; Genotoxicity ; Nuclear medicine ; Original ; Original Article ; Peptides ; Radioisotopes ; Statistical analysis ; Tumors</subject><ispartof>Cytotechnology (Dordrecht), 2016-12, Vol.68 (6), p.2301-2310</ispartof><rights>Springer Science+Business Media Dordrecht 2016</rights><rights>Copyright Springer Science & Business Media 2016</rights><rights>Springer Science+Business Media Dordrecht 2016.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c535t-f3a36023e6cf72a580efa2e4dbcc647623e16dcb48822e69621a2b1ae7224883</citedby><cites>FETCH-LOGICAL-c535t-f3a36023e6cf72a580efa2e4dbcc647623e16dcb48822e69621a2b1ae7224883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5101300/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2918270476?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,21388,27924,27925,33744,33745,41488,42557,43805,51319,53791,53793,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27686814$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ocampo, Ivette Zegarra</creatorcontrib><creatorcontrib>de Queiroz Souza Passos, Priscila</creatorcontrib><creatorcontrib>Ramirez de Carvalho, Luma</creatorcontrib><creatorcontrib>Lira da Cruz, Camila Ayala</creatorcontrib><creatorcontrib>Esteves-Pedro, Natália Mencacci</creatorcontrib><creatorcontrib>Medeiros da Silva, Fabiana</creatorcontrib><creatorcontrib>Higa, Olga Zazuco</creatorcontrib><creatorcontrib>Dias, Luiz Alberto Pereira</creatorcontrib><creatorcontrib>Okazaki, Kayo</creatorcontrib><creatorcontrib>Vieira, Daniel Perez</creatorcontrib><title>In vitro cytotoxic and genotoxic evaluation of peptides used in nuclear medicine (DOTATATE and Ubiquicidin29-41) in CHO-K1 cells</title><title>Cytotechnology (Dordrecht)</title><addtitle>Cytotechnology</addtitle><addtitle>Cytotechnology</addtitle><description>Micronucleus (MN) assay constitutes a valuable surrogate to the chromosome aberration technique for in vitro testing of the genotoxicity of substances. As test substances, two peptidic compounds (DOTATATE and Ubiquicidin
29-41
) used in nuclear medicine, were tested for in vitro cytotoxicity and genotoxicity in CHO-K1 cells. None of the compounds showed detectable cytotoxicity (0.5–7.3 ng/mL for DOTATATE and 0.3–4.5 ng/mL for UBI
29-41
), genotoxicity (0.72, 7.2 and 72.0 ng/ml for DOTATATE and 0.45, 4.5 and 45.0 ng/mL for UBI
29-41
) or cell cycle changes as compared to untreated controls at the concentrations tested. Statistical analysis showed good concordance between two independent analysts. The results corroborate the notion of the safety of the compounds and present improvements of the in vitro MN assay when performed in a pre-clinical trial context that increase the throughput of small-to-medium testing facilities as an alternative to high content screening systems.</description><subject>Automation</subject><subject>Biochemistry</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Cell cycle</subject><subject>Cell division</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chromosome aberrations</subject><subject>Chromosomes</subject><subject>Cytotoxicity</subject><subject>Drug dosages</subject><subject>Genotoxicity</subject><subject>Nuclear medicine</subject><subject>Original</subject><subject>Original Article</subject><subject>Peptides</subject><subject>Radioisotopes</subject><subject>Statistical analysis</subject><subject>Tumors</subject><issn>0920-9069</issn><issn>1573-0778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9Us9rFDEUHkSxa_UP8CIBL_Uw-l4yk0kuQlmrLRb2sp5DJpNZU2aTbTKztDf_dDPuWqqghEdIvh_5HnlF8RrhPQI0HxICR17CXECrUj4pFlg3rISmEU-LBUgKpQQuT4oXKd0AgGyQPS9OaMMFF1gtih9XnuzdGAMx92MYw50zRPuObKw_nuxeD5MeXfAk9GRnd6PrbCJTsh1xnvjJDFZHsrWdM85bcvZptT7P6-KXz7fW3U4Z6Jynsqzw3axZXq7Kr0iMHYb0snjW6yHZV8f9tFh_vlgvL8vr1Zer5fl1aWpWj2XPNONAmeWmb6iuBdheU1t1rTG8anhGkHemrYSg1HLJKWraorYNpfmOnRYfD7a7qc1RjfVj1IPaRbfV8V4F7dSfiHff1SbsVY2ADCAbnB0NYridbBrV1qW5A-1tmJJCwSRihThT3_5FvQlT9Lk7RSUK2kAO_D8WCgGiYhzn3HhgmRhSirZ_iIyg5iFQhyFQMFceAiWz5s3jXh8Uv389E-iBkDLkNzY-evqfrj8BkPC7Dw</recordid><startdate>20161201</startdate><enddate>20161201</enddate><creator>Ocampo, Ivette Zegarra</creator><creator>de Queiroz Souza Passos, Priscila</creator><creator>Ramirez de Carvalho, Luma</creator><creator>Lira da Cruz, Camila Ayala</creator><creator>Esteves-Pedro, Natália Mencacci</creator><creator>Medeiros da Silva, Fabiana</creator><creator>Higa, Olga Zazuco</creator><creator>Dias, Luiz Alberto Pereira</creator><creator>Okazaki, Kayo</creator><creator>Vieira, Daniel Perez</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20161201</creationdate><title>In vitro cytotoxic and genotoxic evaluation of peptides used in nuclear medicine (DOTATATE and Ubiquicidin29-41) in CHO-K1 cells</title><author>Ocampo, Ivette Zegarra ; de Queiroz Souza Passos, Priscila ; Ramirez de Carvalho, Luma ; Lira da Cruz, Camila Ayala ; Esteves-Pedro, Natália Mencacci ; Medeiros da Silva, Fabiana ; Higa, Olga Zazuco ; Dias, Luiz Alberto Pereira ; Okazaki, Kayo ; Vieira, Daniel Perez</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c535t-f3a36023e6cf72a580efa2e4dbcc647623e16dcb48822e69621a2b1ae7224883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Automation</topic><topic>Biochemistry</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Cell cycle</topic><topic>Cell division</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chromosome aberrations</topic><topic>Chromosomes</topic><topic>Cytotoxicity</topic><topic>Drug dosages</topic><topic>Genotoxicity</topic><topic>Nuclear medicine</topic><topic>Original</topic><topic>Original Article</topic><topic>Peptides</topic><topic>Radioisotopes</topic><topic>Statistical analysis</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ocampo, Ivette Zegarra</creatorcontrib><creatorcontrib>de Queiroz Souza Passos, Priscila</creatorcontrib><creatorcontrib>Ramirez de Carvalho, Luma</creatorcontrib><creatorcontrib>Lira da Cruz, Camila Ayala</creatorcontrib><creatorcontrib>Esteves-Pedro, Natália Mencacci</creatorcontrib><creatorcontrib>Medeiros da Silva, Fabiana</creatorcontrib><creatorcontrib>Higa, Olga Zazuco</creatorcontrib><creatorcontrib>Dias, Luiz Alberto Pereira</creatorcontrib><creatorcontrib>Okazaki, Kayo</creatorcontrib><creatorcontrib>Vieira, Daniel Perez</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cytotechnology (Dordrecht)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ocampo, Ivette Zegarra</au><au>de Queiroz Souza Passos, Priscila</au><au>Ramirez de Carvalho, Luma</au><au>Lira da Cruz, Camila Ayala</au><au>Esteves-Pedro, Natália Mencacci</au><au>Medeiros da Silva, Fabiana</au><au>Higa, Olga Zazuco</au><au>Dias, Luiz Alberto Pereira</au><au>Okazaki, Kayo</au><au>Vieira, Daniel Perez</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro cytotoxic and genotoxic evaluation of peptides used in nuclear medicine (DOTATATE and Ubiquicidin29-41) in CHO-K1 cells</atitle><jtitle>Cytotechnology (Dordrecht)</jtitle><stitle>Cytotechnology</stitle><addtitle>Cytotechnology</addtitle><date>2016-12-01</date><risdate>2016</risdate><volume>68</volume><issue>6</issue><spage>2301</spage><epage>2310</epage><pages>2301-2310</pages><issn>0920-9069</issn><eissn>1573-0778</eissn><abstract>Micronucleus (MN) assay constitutes a valuable surrogate to the chromosome aberration technique for in vitro testing of the genotoxicity of substances. As test substances, two peptidic compounds (DOTATATE and Ubiquicidin
29-41
) used in nuclear medicine, were tested for in vitro cytotoxicity and genotoxicity in CHO-K1 cells. None of the compounds showed detectable cytotoxicity (0.5–7.3 ng/mL for DOTATATE and 0.3–4.5 ng/mL for UBI
29-41
), genotoxicity (0.72, 7.2 and 72.0 ng/ml for DOTATATE and 0.45, 4.5 and 45.0 ng/mL for UBI
29-41
) or cell cycle changes as compared to untreated controls at the concentrations tested. Statistical analysis showed good concordance between two independent analysts. The results corroborate the notion of the safety of the compounds and present improvements of the in vitro MN assay when performed in a pre-clinical trial context that increase the throughput of small-to-medium testing facilities as an alternative to high content screening systems.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>27686814</pmid><doi>10.1007/s10616-016-0024-9</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | SpringerLink Journals; EZB-FREE-00999 freely available EZB journals; ProQuest Central UK/Ireland; PubMed Central; ProQuest Central |
| subjects | Automation Biochemistry Biomedicine Biotechnology Cell cycle Cell division Chemistry Chemistry and Materials Science Chromosome aberrations Chromosomes Cytotoxicity Drug dosages Genotoxicity Nuclear medicine Original Original Article Peptides Radioisotopes Statistical analysis Tumors |
| title | In vitro cytotoxic and genotoxic evaluation of peptides used in nuclear medicine (DOTATATE and Ubiquicidin29-41) in CHO-K1 cells |
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