Simultaneous measurement of mitochondrial respiration and ATP production in tissue homogenates and calculation of effective P/O ratios

The use of tissue homogenate has greatly aided the study of the functioning of mitochondria. However, the amount of ATP produced per oxygen molecule consumed, that is, the effective P/O ratio, has never been measured directly in tissue homogenate. Here we combine and refine existing methods previous...

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Veröffentlicht in:	Physiological reports 2016-10, Vol.4 (20), p.np-n/a
Hauptverfasser:	Salin, Karine, Villasevil, Eugenia M., Auer, Sonya K., Anderson, Graeme J., Selman, Colin, Metcalfe, Neil B., Chinopoulos, Christos
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine triphosphatase Adenosine Triphosphatases - metabolism Adenosine Triphosphate - metabolism Animals ATPase Bioenergetics Cellular Physiology Centrifugation Dietary restrictions Electron transport fluorescence Hydrolysis Liver magnesium green Metabolic Pathways Methods Mitochondria Mitochondria - metabolism Mitochondria - physiology Mitochondria, Liver - metabolism Original Research Oxidative Phosphorylation Oxygen - metabolism Oxygen consumption Oxygen Consumption - physiology oxygraph Physiology Respiration Salmo trutta Trout
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