Transmission of atypical scrapie to homozygous ARQ sheep
Two Cheviot ewes homozygous for the A136L141R154Q171 (AL141RQ) prion protein (PrP) genotype were exposed intracerebrally to brain pools prepared using four field cases of atypical scrapie from the United Kingdom. Animals were clinically normal until the end of the experiment, when they were culled 7...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2016, Vol.78(10), pp.1619-1624 |
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| container_title | Journal of Veterinary Medical Science |
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| creator | OKADA, Hiroyuki MIYAZAWA, Kohtaro IMAMURA, Morikazu IWAMARU, Yoshifumi MASUJIN, Kentaro MATSUURA, Yuichi YOKOYAMA, Takashi |
| description | Two Cheviot ewes homozygous for the A136L141R154Q171 (AL141RQ) prion protein (PrP) genotype were exposed intracerebrally to brain pools prepared using four field cases of atypical scrapie from the United Kingdom. Animals were clinically normal until the end of the experiment, when they were culled 7 years post-inoculation. Limited accumulation of disease-associated PrP (PrPSc) was observed in the cerebellar molecular layer by immunohistochemistry, but not by western blot or enzyme-linked immunosorbent assay. In addition, PrPSc was partially localized in astrocytes and microglia, suggesting that these cells have a role in PrPSc processing, degradation or both. Our results indicate that atypical scrapie is transmissible to AL141RQ sheep, but these animals act as clinically silent carriers with long incubation times. |
| doi_str_mv | 10.1292/jvms.16-0259 |
| format | Article |
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Animals were clinically normal until the end of the experiment, when they were culled 7 years post-inoculation. Limited accumulation of disease-associated PrP (PrPSc) was observed in the cerebellar molecular layer by immunohistochemistry, but not by western blot or enzyme-linked immunosorbent assay. In addition, PrPSc was partially localized in astrocytes and microglia, suggesting that these cells have a role in PrPSc processing, degradation or both. Our results indicate that atypical scrapie is transmissible to AL141RQ sheep, but these animals act as clinically silent carriers with long incubation times.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.16-0259</identifier><identifier>PMID: 27320968</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Animals ; ARQ allele ; Astrocytes ; atypical scrapie ; Brain ; Brain - pathology ; Cerebellum ; Degradation ; Enzyme-linked immunosorbent assay ; Female ; Homozygote ; Immunohistochemistry ; Immunohistochemistry - veterinary ; Infectious Disease Incubation Period ; Inoculation ; Microglia ; Pathology ; prion ; Prion protein ; Prion Proteins - genetics ; Scrapie ; Scrapie - genetics ; Scrapie - pathology ; Scrapie - transmission ; Sheep ; Sheep - genetics ; transmission</subject><ispartof>Journal of Veterinary Medical Science, 2016, Vol.78(10), pp.1619-1624</ispartof><rights>2016 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency 2016</rights><rights>2016 The Japanese Society of Veterinary Science 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c734t-477f2339dc8070305077637140130c61725db4712567f242185a066b5f19e5553</citedby><cites>FETCH-LOGICAL-c734t-477f2339dc8070305077637140130c61725db4712567f242185a066b5f19e5553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5095634/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5095634/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1877,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27320968$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>OKADA, Hiroyuki</creatorcontrib><creatorcontrib>MIYAZAWA, Kohtaro</creatorcontrib><creatorcontrib>IMAMURA, Morikazu</creatorcontrib><creatorcontrib>IWAMARU, Yoshifumi</creatorcontrib><creatorcontrib>MASUJIN, Kentaro</creatorcontrib><creatorcontrib>MATSUURA, Yuichi</creatorcontrib><creatorcontrib>YOKOYAMA, Takashi</creatorcontrib><title>Transmission of atypical scrapie to homozygous ARQ sheep</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>Two Cheviot ewes homozygous for the A136L141R154Q171 (AL141RQ) prion protein (PrP) genotype were exposed intracerebrally to brain pools prepared using four field cases of atypical scrapie from the United Kingdom. Animals were clinically normal until the end of the experiment, when they were culled 7 years post-inoculation. Limited accumulation of disease-associated PrP (PrPSc) was observed in the cerebellar molecular layer by immunohistochemistry, but not by western blot or enzyme-linked immunosorbent assay. In addition, PrPSc was partially localized in astrocytes and microglia, suggesting that these cells have a role in PrPSc processing, degradation or both. Our results indicate that atypical scrapie is transmissible to AL141RQ sheep, but these animals act as clinically silent carriers with long incubation times.</description><subject>Animals</subject><subject>ARQ allele</subject><subject>Astrocytes</subject><subject>atypical scrapie</subject><subject>Brain</subject><subject>Brain - pathology</subject><subject>Cerebellum</subject><subject>Degradation</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Female</subject><subject>Homozygote</subject><subject>Immunohistochemistry</subject><subject>Immunohistochemistry - veterinary</subject><subject>Infectious Disease Incubation Period</subject><subject>Inoculation</subject><subject>Microglia</subject><subject>Pathology</subject><subject>prion</subject><subject>Prion protein</subject><subject>Prion Proteins - genetics</subject><subject>Scrapie</subject><subject>Scrapie - genetics</subject><subject>Scrapie - pathology</subject><subject>Scrapie - transmission</subject><subject>Sheep</subject><subject>Sheep - genetics</subject><subject>transmission</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkV1L5DAUhoMo6zi7d15LwRsvrJ58NzeCDH4sCMvK7HXIZNKZDm1Tk44w_vpNqQ7qTQ7kPDyclxehUwxXmChyvXlt4hUWORCuDtAEUyZzyag6RBNQ6V8SDsfoJMYNAMFMqB_omEhKQIligop5MG1sqhgr32a-zEy_6ypr6izaYLrKZb3P1r7xb7uV38bs9vlvFtfOdT_RUWnq6H69zyn6d383nz3mT38efs9un3IrKetzJmVJKFVLW4AEChykFFRiBpiCFThdt1wwiQkXCWQEF9yAEAteYuU453SKbkZvt100bmld2wdT6y5UjQk77U2lv27aaq1X_lVzUFxQlgQX74LgX7Yu9jqlta6uTetSIo0LIiSAIpDQ82_oxm9Dm-IligrKFVM0UZcjZYOPMbhyfwwGPVSih0o0FnqoJOFnnwPs4Y8OEjAbgU3szcrtARP6ytZutMlisA_vqN1v7doE7Vr6H-9rnWE</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>OKADA, Hiroyuki</creator><creator>MIYAZAWA, Kohtaro</creator><creator>IMAMURA, Morikazu</creator><creator>IWAMARU, Yoshifumi</creator><creator>MASUJIN, Kentaro</creator><creator>MATSUURA, Yuichi</creator><creator>YOKOYAMA, Takashi</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Veterinary Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2016</creationdate><title>Transmission of atypical scrapie to homozygous ARQ sheep</title><author>OKADA, Hiroyuki ; MIYAZAWA, Kohtaro ; IMAMURA, Morikazu ; IWAMARU, Yoshifumi ; MASUJIN, Kentaro ; MATSUURA, Yuichi ; YOKOYAMA, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c734t-477f2339dc8070305077637140130c61725db4712567f242185a066b5f19e5553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>ARQ allele</topic><topic>Astrocytes</topic><topic>atypical scrapie</topic><topic>Brain</topic><topic>Brain - pathology</topic><topic>Cerebellum</topic><topic>Degradation</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Female</topic><topic>Homozygote</topic><topic>Immunohistochemistry</topic><topic>Immunohistochemistry - veterinary</topic><topic>Infectious Disease Incubation Period</topic><topic>Inoculation</topic><topic>Microglia</topic><topic>Pathology</topic><topic>prion</topic><topic>Prion protein</topic><topic>Prion Proteins - genetics</topic><topic>Scrapie</topic><topic>Scrapie - genetics</topic><topic>Scrapie - pathology</topic><topic>Scrapie - transmission</topic><topic>Sheep</topic><topic>Sheep - genetics</topic><topic>transmission</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OKADA, Hiroyuki</creatorcontrib><creatorcontrib>MIYAZAWA, Kohtaro</creatorcontrib><creatorcontrib>IMAMURA, Morikazu</creatorcontrib><creatorcontrib>IWAMARU, Yoshifumi</creatorcontrib><creatorcontrib>MASUJIN, Kentaro</creatorcontrib><creatorcontrib>MATSUURA, Yuichi</creatorcontrib><creatorcontrib>YOKOYAMA, Takashi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OKADA, Hiroyuki</au><au>MIYAZAWA, Kohtaro</au><au>IMAMURA, Morikazu</au><au>IWAMARU, Yoshifumi</au><au>MASUJIN, Kentaro</au><au>MATSUURA, Yuichi</au><au>YOKOYAMA, Takashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transmission of atypical scrapie to homozygous ARQ sheep</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2016</date><risdate>2016</risdate><volume>78</volume><issue>10</issue><spage>1619</spage><epage>1624</epage><pages>1619-1624</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>Two Cheviot ewes homozygous for the A136L141R154Q171 (AL141RQ) prion protein (PrP) genotype were exposed intracerebrally to brain pools prepared using four field cases of atypical scrapie from the United Kingdom. Animals were clinically normal until the end of the experiment, when they were culled 7 years post-inoculation. Limited accumulation of disease-associated PrP (PrPSc) was observed in the cerebellar molecular layer by immunohistochemistry, but not by western blot or enzyme-linked immunosorbent assay. In addition, PrPSc was partially localized in astrocytes and microglia, suggesting that these cells have a role in PrPSc processing, degradation or both. Our results indicate that atypical scrapie is transmissible to AL141RQ sheep, but these animals act as clinically silent carriers with long incubation times.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>27320968</pmid><doi>10.1292/jvms.16-0259</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals ARQ allele Astrocytes atypical scrapie Brain Brain - pathology Cerebellum Degradation Enzyme-linked immunosorbent assay Female Homozygote Immunohistochemistry Immunohistochemistry - veterinary Infectious Disease Incubation Period Inoculation Microglia Pathology prion Prion protein Prion Proteins - genetics Scrapie Scrapie - genetics Scrapie - pathology Scrapie - transmission Sheep Sheep - genetics transmission |
| title | Transmission of atypical scrapie to homozygous ARQ sheep |
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