How many papillomavirus species can go undetected in papilloma lesions?
A co-infection comprising to at least seven papillomavirus (PV) types was detected by next generation sequencing (NGS) of randomly primed rolling circle amplification (RCA) products of a bovine ( Bos taurus ) papilloma lesion from the Brazilian Amazon region. Six putative new PV types that could not...
Gespeichert in:
| Veröffentlicht in: | Scientific reports 2016-11, Vol.6 (1), p.36480-36480, Article 36480 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 36480 |
|---|---|
| container_issue | 1 |
| container_start_page | 36480 |
| container_title | Scientific reports |
| container_volume | 6 |
| creator | Daudt, Cíntia da Silva, Flavio R. C. Streck, André F. Weber, Matheus N. Mayer, Fabiana Q. Cibulski, Samuel P. Canal, Cláudio W. |
| description | A co-infection comprising to at least seven papillomavirus (PV) types was detected by next generation sequencing (NGS) of randomly primed rolling circle amplification (RCA) products of a bovine (
Bos taurus
) papilloma lesion from the Brazilian Amazon region. Six putative new PV types that could not be detected by commonly used PCR protocols were identified. Their overall L1 nucleotide identities were less than 90% compared to described PV species and types. L1 nucleotide BLAST sequence hits showed that each new type was related to
Beta, Gamma
,
Dyokappa
,
Dyoeta
, and
Xipapillomavirus
, as well as two likely new unclassified genera. Our results show that the employment of NGS is relevant to the detection and characterization of distantly related PV and is of major importance in co-infection studies. This knowledge will help us understand the biology and pathogenesis of PV, as well as contribute to disease control. Moreover, we can also conclude that there are many unknown circulating PVs. |
| doi_str_mv | 10.1038/srep36480 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5093584</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1899329504</sourcerecordid><originalsourceid>FETCH-LOGICAL-c438t-65fb0fa97a0c4b93188af408cd26027d0eb492f32b2e6c0f0bc9a3febd50e82e3</originalsourceid><addsrcrecordid>eNpl0U9LwzAYBvAgioru4BeQghcVpm-Tpksuioj_YOBFzyFN385Im9Sknfjt7ZjOqbkkkB9P3vAQcpDCWQpMnMeALcszARtkl0LGx5RRurl23iGjGF9hWJzKLJXbZIdOBAjK-S65u_fvSaPdR9Lq1ta1b_Tchj4msUVjMSZGu2Tmk96V2KHpsEys-7FJjdF6Fy_3yVal64ijr32PPN_ePF3fj6ePdw_XV9OxyZjoxjmvCqi0nGgwWSFZKoSuMhCmpDnQSQlYZJJWjBYUcwMVFEZqVmFRckBBke2Ri2Vu2xcNlgZdF3St2mAbHT6U11b9vnH2Rc38XHGQjItsCDj-Cgj-rcfYqcZGg3WtHfo-qlSwfMI4Txf06A999X1ww_cGJSWjksNCnSyVCT4OZVSrYVJQi4bUqqHBHq5Pv5LffQzgdAnicOVmGNae_Jf2CS-7m1U</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1899329504</pqid></control><display><type>article</type><title>How many papillomavirus species can go undetected in papilloma lesions?</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Springer Nature OA Free Journals</source><source>Nature Free</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Daudt, Cíntia ; da Silva, Flavio R. C. ; Streck, André F. ; Weber, Matheus N. ; Mayer, Fabiana Q. ; Cibulski, Samuel P. ; Canal, Cláudio W.</creator><creatorcontrib>Daudt, Cíntia ; da Silva, Flavio R. C. ; Streck, André F. ; Weber, Matheus N. ; Mayer, Fabiana Q. ; Cibulski, Samuel P. ; Canal, Cláudio W.</creatorcontrib><description>A co-infection comprising to at least seven papillomavirus (PV) types was detected by next generation sequencing (NGS) of randomly primed rolling circle amplification (RCA) products of a bovine (
Bos taurus
) papilloma lesion from the Brazilian Amazon region. Six putative new PV types that could not be detected by commonly used PCR protocols were identified. Their overall L1 nucleotide identities were less than 90% compared to described PV species and types. L1 nucleotide BLAST sequence hits showed that each new type was related to
Beta, Gamma
,
Dyokappa
,
Dyoeta
, and
Xipapillomavirus
, as well as two likely new unclassified genera. Our results show that the employment of NGS is relevant to the detection and characterization of distantly related PV and is of major importance in co-infection studies. This knowledge will help us understand the biology and pathogenesis of PV, as well as contribute to disease control. Moreover, we can also conclude that there are many unknown circulating PVs.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep36480</identifier><identifier>PMID: 27808255</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>49/23 ; 49/47 ; 631/326/325 ; 631/326/596 ; Animals ; Base Sequence ; Bos taurus ; Cattle ; Coinfection - veterinary ; Coinfection - virology ; Disease control ; DNA, Viral - chemistry ; DNA, Viral - isolation & purification ; DNA, Viral - metabolism ; Genetic diversity ; Genome, Viral ; Genomes ; High-Throughput Nucleotide Sequencing ; Human papillomavirus ; Humanities and Social Sciences ; Infections ; Lesions ; multidisciplinary ; Nucleic Acid Amplification Techniques ; Open Reading Frames - genetics ; Papilloma ; Papilloma - pathology ; Papilloma - veterinary ; Papilloma - virology ; Papillomaviridae ; Papillomaviridae - classification ; Papillomaviridae - genetics ; Papillomaviridae - isolation & purification ; Pathogenesis ; Phylogenetics ; Phylogeny ; Polymerase Chain Reaction ; Science ; Sequence Analysis, DNA ; Tumors</subject><ispartof>Scientific reports, 2016-11, Vol.6 (1), p.36480-36480, Article 36480</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Nature Publishing Group Nov 2016</rights><rights>Copyright © 2016, The Author(s) 2016 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-65fb0fa97a0c4b93188af408cd26027d0eb492f32b2e6c0f0bc9a3febd50e82e3</citedby><cites>FETCH-LOGICAL-c438t-65fb0fa97a0c4b93188af408cd26027d0eb492f32b2e6c0f0bc9a3febd50e82e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5093584/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5093584/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,27907,27908,41103,42172,51559,53774,53776</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27808255$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Daudt, Cíntia</creatorcontrib><creatorcontrib>da Silva, Flavio R. C.</creatorcontrib><creatorcontrib>Streck, André F.</creatorcontrib><creatorcontrib>Weber, Matheus N.</creatorcontrib><creatorcontrib>Mayer, Fabiana Q.</creatorcontrib><creatorcontrib>Cibulski, Samuel P.</creatorcontrib><creatorcontrib>Canal, Cláudio W.</creatorcontrib><title>How many papillomavirus species can go undetected in papilloma lesions?</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>A co-infection comprising to at least seven papillomavirus (PV) types was detected by next generation sequencing (NGS) of randomly primed rolling circle amplification (RCA) products of a bovine (
Bos taurus
) papilloma lesion from the Brazilian Amazon region. Six putative new PV types that could not be detected by commonly used PCR protocols were identified. Their overall L1 nucleotide identities were less than 90% compared to described PV species and types. L1 nucleotide BLAST sequence hits showed that each new type was related to
Beta, Gamma
,
Dyokappa
,
Dyoeta
, and
Xipapillomavirus
, as well as two likely new unclassified genera. Our results show that the employment of NGS is relevant to the detection and characterization of distantly related PV and is of major importance in co-infection studies. This knowledge will help us understand the biology and pathogenesis of PV, as well as contribute to disease control. Moreover, we can also conclude that there are many unknown circulating PVs.</description><subject>49/23</subject><subject>49/47</subject><subject>631/326/325</subject><subject>631/326/596</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Bos taurus</subject><subject>Cattle</subject><subject>Coinfection - veterinary</subject><subject>Coinfection - virology</subject><subject>Disease control</subject><subject>DNA, Viral - chemistry</subject><subject>DNA, Viral - isolation & purification</subject><subject>DNA, Viral - metabolism</subject><subject>Genetic diversity</subject><subject>Genome, Viral</subject><subject>Genomes</subject><subject>High-Throughput Nucleotide Sequencing</subject><subject>Human papillomavirus</subject><subject>Humanities and Social Sciences</subject><subject>Infections</subject><subject>Lesions</subject><subject>multidisciplinary</subject><subject>Nucleic Acid Amplification Techniques</subject><subject>Open Reading Frames - genetics</subject><subject>Papilloma</subject><subject>Papilloma - pathology</subject><subject>Papilloma - veterinary</subject><subject>Papilloma - virology</subject><subject>Papillomaviridae</subject><subject>Papillomaviridae - classification</subject><subject>Papillomaviridae - genetics</subject><subject>Papillomaviridae - isolation & purification</subject><subject>Pathogenesis</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>Science</subject><subject>Sequence Analysis, DNA</subject><subject>Tumors</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpl0U9LwzAYBvAgioru4BeQghcVpm-Tpksuioj_YOBFzyFN385Im9Sknfjt7ZjOqbkkkB9P3vAQcpDCWQpMnMeALcszARtkl0LGx5RRurl23iGjGF9hWJzKLJXbZIdOBAjK-S65u_fvSaPdR9Lq1ta1b_Tchj4msUVjMSZGu2Tmk96V2KHpsEys-7FJjdF6Fy_3yVal64ijr32PPN_ePF3fj6ePdw_XV9OxyZjoxjmvCqi0nGgwWSFZKoSuMhCmpDnQSQlYZJJWjBYUcwMVFEZqVmFRckBBke2Ri2Vu2xcNlgZdF3St2mAbHT6U11b9vnH2Rc38XHGQjItsCDj-Cgj-rcfYqcZGg3WtHfo-qlSwfMI4Txf06A999X1ww_cGJSWjksNCnSyVCT4OZVSrYVJQi4bUqqHBHq5Pv5LffQzgdAnicOVmGNae_Jf2CS-7m1U</recordid><startdate>20161103</startdate><enddate>20161103</enddate><creator>Daudt, Cíntia</creator><creator>da Silva, Flavio R. C.</creator><creator>Streck, André F.</creator><creator>Weber, Matheus N.</creator><creator>Mayer, Fabiana Q.</creator><creator>Cibulski, Samuel P.</creator><creator>Canal, Cláudio W.</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20161103</creationdate><title>How many papillomavirus species can go undetected in papilloma lesions?</title><author>Daudt, Cíntia ; da Silva, Flavio R. C. ; Streck, André F. ; Weber, Matheus N. ; Mayer, Fabiana Q. ; Cibulski, Samuel P. ; Canal, Cláudio W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-65fb0fa97a0c4b93188af408cd26027d0eb492f32b2e6c0f0bc9a3febd50e82e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>49/23</topic><topic>49/47</topic><topic>631/326/325</topic><topic>631/326/596</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Bos taurus</topic><topic>Cattle</topic><topic>Coinfection - veterinary</topic><topic>Coinfection - virology</topic><topic>Disease control</topic><topic>DNA, Viral - chemistry</topic><topic>DNA, Viral - isolation & purification</topic><topic>DNA, Viral - metabolism</topic><topic>Genetic diversity</topic><topic>Genome, Viral</topic><topic>Genomes</topic><topic>High-Throughput Nucleotide Sequencing</topic><topic>Human papillomavirus</topic><topic>Humanities and Social Sciences</topic><topic>Infections</topic><topic>Lesions</topic><topic>multidisciplinary</topic><topic>Nucleic Acid Amplification Techniques</topic><topic>Open Reading Frames - genetics</topic><topic>Papilloma</topic><topic>Papilloma - pathology</topic><topic>Papilloma - veterinary</topic><topic>Papilloma - virology</topic><topic>Papillomaviridae</topic><topic>Papillomaviridae - classification</topic><topic>Papillomaviridae - genetics</topic><topic>Papillomaviridae - isolation & purification</topic><topic>Pathogenesis</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>Science</topic><topic>Sequence Analysis, DNA</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Daudt, Cíntia</creatorcontrib><creatorcontrib>da Silva, Flavio R. C.</creatorcontrib><creatorcontrib>Streck, André F.</creatorcontrib><creatorcontrib>Weber, Matheus N.</creatorcontrib><creatorcontrib>Mayer, Fabiana Q.</creatorcontrib><creatorcontrib>Cibulski, Samuel P.</creatorcontrib><creatorcontrib>Canal, Cláudio W.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Daudt, Cíntia</au><au>da Silva, Flavio R. C.</au><au>Streck, André F.</au><au>Weber, Matheus N.</au><au>Mayer, Fabiana Q.</au><au>Cibulski, Samuel P.</au><au>Canal, Cláudio W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>How many papillomavirus species can go undetected in papilloma lesions?</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-11-03</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>36480</spage><epage>36480</epage><pages>36480-36480</pages><artnum>36480</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>A co-infection comprising to at least seven papillomavirus (PV) types was detected by next generation sequencing (NGS) of randomly primed rolling circle amplification (RCA) products of a bovine (
Bos taurus
) papilloma lesion from the Brazilian Amazon region. Six putative new PV types that could not be detected by commonly used PCR protocols were identified. Their overall L1 nucleotide identities were less than 90% compared to described PV species and types. L1 nucleotide BLAST sequence hits showed that each new type was related to
Beta, Gamma
,
Dyokappa
,
Dyoeta
, and
Xipapillomavirus
, as well as two likely new unclassified genera. Our results show that the employment of NGS is relevant to the detection and characterization of distantly related PV and is of major importance in co-infection studies. This knowledge will help us understand the biology and pathogenesis of PV, as well as contribute to disease control. Moreover, we can also conclude that there are many unknown circulating PVs.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27808255</pmid><doi>10.1038/srep36480</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2016-11, Vol.6 (1), p.36480-36480, Article 36480 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5093584 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Springer Nature OA Free Journals; Nature Free; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 49/23 49/47 631/326/325 631/326/596 Animals Base Sequence Bos taurus Cattle Coinfection - veterinary Coinfection - virology Disease control DNA, Viral - chemistry DNA, Viral - isolation & purification DNA, Viral - metabolism Genetic diversity Genome, Viral Genomes High-Throughput Nucleotide Sequencing Human papillomavirus Humanities and Social Sciences Infections Lesions multidisciplinary Nucleic Acid Amplification Techniques Open Reading Frames - genetics Papilloma Papilloma - pathology Papilloma - veterinary Papilloma - virology Papillomaviridae Papillomaviridae - classification Papillomaviridae - genetics Papillomaviridae - isolation & purification Pathogenesis Phylogenetics Phylogeny Polymerase Chain Reaction Science Sequence Analysis, DNA Tumors |
| title | How many papillomavirus species can go undetected in papilloma lesions? |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T21%3A12%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=How%20many%20papillomavirus%20species%20can%20go%20undetected%20in%20papilloma%20lesions?&rft.jtitle=Scientific%20reports&rft.au=Daudt,%20C%C3%ADntia&rft.date=2016-11-03&rft.volume=6&rft.issue=1&rft.spage=36480&rft.epage=36480&rft.pages=36480-36480&rft.artnum=36480&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/srep36480&rft_dat=%3Cproquest_pubme%3E1899329504%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1899329504&rft_id=info:pmid/27808255&rfr_iscdi=true |