Shape-Memory Terpolymer Rods with 17-β-estradiol for the Treatment of Neurodegenerative Diseases: an In Vitro and In Vivo Study
Purpose Estradiol (E2)-loaded poly(L-lactide-co-glycolide-trimethylenecarbonate) (P(L-LA:GA:TMC)) rods with shape-memory were developed for the treatment of neurodegenerative diseases. Usefulness of the extrusion method in the obtaining process was also considered. The influence of structural and su...
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| Veröffentlicht in: | Pharmaceutical research 2016-12, Vol.33 (12), p.2967-2978 |
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| creator | Turek, Artur Olakowska, Edyta Borecka, Aleksandra Janeczek, Henryk Sobota, Michał Jaworska, Joanna Kaczmarczyk, Bożena Jarząbek, Bożena Gruchlik, Arkadiusz Libera, Marcin Liśkiewicz, Arkadiusz Jędrzejowska-Szypułka, Halina Kasperczyk, Janusz |
| description | Purpose
Estradiol (E2)-loaded poly(L-lactide-co-glycolide-trimethylenecarbonate) (P(L-LA:GA:TMC)) rods with shape-memory were developed for the treatment of neurodegenerative diseases. Usefulness of the extrusion method in the obtaining process was also considered. The influence of structural and surface properties during hydrolytic degradation was developed. The possible therapeutic aspect of rods with E2 was determined.
Methods
The extruded rods were incubated in a PBS solution (pH 7.4, 37°C, 240 rpm). The amount of released E2
in vitro
conditions was estimated by UV-VIS method. The following methods in the degradation of rods were applied: NMR, DSC, FTIR, GPC, SEM, and optical microscopy. Changes in water uptake and weight loss were also determined.
In vivo
study was performed on rats. Measurements of E2 level were performed before and after ovariectomy of rats using ELISA method. A sample of tissue adjacent to the site of the rod implantation was analysed under an optical microscope.
Results
A stable and steady degradation process ensured zero-order release of E2. The
in vivo
study indicated a significant increase in the E2 level in serum after ovariectomy. Moreover, structural and surface features indicated that the extrusion method was appropriate for obtaining E2-loaded rods.
Conclusions
Shape-memory P(L-LA:GA:TMC) rods with E2 are an adequate proposal for further research in the field of neurological disorders. |
| doi_str_mv | 10.1007/s11095-016-2019-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5093210</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1836728588</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-d2c741d0e6111c15afb9e585ea85584f4110c1aff881a8ff0af07a233d2445f63</originalsourceid><addsrcrecordid>eNqNkc9u1DAQxi0EokvhAbggH7kYPI4dOxyQUPlXqYBEF8TNcuPxbqokXuxk0d54Jh6EZ8JVSgUXxMljzTefZr4fIQ-BPwHO9dMMwBvFONRMcGhYc4usQOmKNVx-uU1WXAvJjJZwRO7lfMk5N9DIu-RI6FqYWqgV-X6-dTtk73CI6UDXmHaxPwyY6MfoM_3WTVsKmv38wTBPyfku9jTERKct0nVCNw04TjQG-h7nFD1ucMTkpm6P9GWX0WXMz6gb6elIP3dTiqX2y2cf6fk0-8N9cie4PuOD6_eYfHr9an3ylp19eHN68uKMtVKriXnRljs8xxoAWlAuXDSojEJnlDIyyBJFCy4EY8CZELgLXDtRVV5IqUJdHZPni-9uvhjQt2Xv5Hq7S93g0sFG19m_O2O3tZu4t4o3lQBeDB5fG6T4dS5x2KHLLfa9GzHO2YJRXJdQQf6HtKq1MMqYIoVF2qaYc8JwsxFwewXZLpBtgWyvINumzDz685Sbid9Ui0Asglxa4waTvYxzGku8_3D9Bd8PtE4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1836728588</pqid></control><display><type>article</type><title>Shape-Memory Terpolymer Rods with 17-β-estradiol for the Treatment of Neurodegenerative Diseases: an In Vitro and In Vivo Study</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Turek, Artur ; Olakowska, Edyta ; Borecka, Aleksandra ; Janeczek, Henryk ; Sobota, Michał ; Jaworska, Joanna ; Kaczmarczyk, Bożena ; Jarząbek, Bożena ; Gruchlik, Arkadiusz ; Libera, Marcin ; Liśkiewicz, Arkadiusz ; Jędrzejowska-Szypułka, Halina ; Kasperczyk, Janusz</creator><creatorcontrib>Turek, Artur ; Olakowska, Edyta ; Borecka, Aleksandra ; Janeczek, Henryk ; Sobota, Michał ; Jaworska, Joanna ; Kaczmarczyk, Bożena ; Jarząbek, Bożena ; Gruchlik, Arkadiusz ; Libera, Marcin ; Liśkiewicz, Arkadiusz ; Jędrzejowska-Szypułka, Halina ; Kasperczyk, Janusz</creatorcontrib><description>Purpose
Estradiol (E2)-loaded poly(L-lactide-co-glycolide-trimethylenecarbonate) (P(L-LA:GA:TMC)) rods with shape-memory were developed for the treatment of neurodegenerative diseases. Usefulness of the extrusion method in the obtaining process was also considered. The influence of structural and surface properties during hydrolytic degradation was developed. The possible therapeutic aspect of rods with E2 was determined.
Methods
The extruded rods were incubated in a PBS solution (pH 7.4, 37°C, 240 rpm). The amount of released E2
in vitro
conditions was estimated by UV-VIS method. The following methods in the degradation of rods were applied: NMR, DSC, FTIR, GPC, SEM, and optical microscopy. Changes in water uptake and weight loss were also determined.
In vivo
study was performed on rats. Measurements of E2 level were performed before and after ovariectomy of rats using ELISA method. A sample of tissue adjacent to the site of the rod implantation was analysed under an optical microscope.
Results
A stable and steady degradation process ensured zero-order release of E2. The
in vivo
study indicated a significant increase in the E2 level in serum after ovariectomy. Moreover, structural and surface features indicated that the extrusion method was appropriate for obtaining E2-loaded rods.
Conclusions
Shape-memory P(L-LA:GA:TMC) rods with E2 are an adequate proposal for further research in the field of neurological disorders.</description><identifier>ISSN: 0724-8741</identifier><identifier>EISSN: 1573-904X</identifier><identifier>DOI: 10.1007/s11095-016-2019-9</identifier><identifier>PMID: 27628625</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Biochemistry ; Biomedical and Life Sciences ; Biomedical Engineering and Bioengineering ; Biomedicine ; Drug Delivery Systems ; Drug Liberation ; Estradiol - administration & dosage ; Estradiol - chemistry ; Estradiol - pharmacokinetics ; Female ; Hydrolysis ; Medical Law ; Nanotubes - chemistry ; Neurodegenerative Diseases - drug therapy ; Pharmacology/Toxicology ; Pharmacy ; Polyesters - chemistry ; Rats, Wistar ; Research Paper ; Surface Properties ; Tissue Distribution</subject><ispartof>Pharmaceutical research, 2016-12, Vol.33 (12), p.2967-2978</ispartof><rights>The Author(s) 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-d2c741d0e6111c15afb9e585ea85584f4110c1aff881a8ff0af07a233d2445f63</citedby><cites>FETCH-LOGICAL-c475t-d2c741d0e6111c15afb9e585ea85584f4110c1aff881a8ff0af07a233d2445f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11095-016-2019-9$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11095-016-2019-9$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27628625$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Turek, Artur</creatorcontrib><creatorcontrib>Olakowska, Edyta</creatorcontrib><creatorcontrib>Borecka, Aleksandra</creatorcontrib><creatorcontrib>Janeczek, Henryk</creatorcontrib><creatorcontrib>Sobota, Michał</creatorcontrib><creatorcontrib>Jaworska, Joanna</creatorcontrib><creatorcontrib>Kaczmarczyk, Bożena</creatorcontrib><creatorcontrib>Jarząbek, Bożena</creatorcontrib><creatorcontrib>Gruchlik, Arkadiusz</creatorcontrib><creatorcontrib>Libera, Marcin</creatorcontrib><creatorcontrib>Liśkiewicz, Arkadiusz</creatorcontrib><creatorcontrib>Jędrzejowska-Szypułka, Halina</creatorcontrib><creatorcontrib>Kasperczyk, Janusz</creatorcontrib><title>Shape-Memory Terpolymer Rods with 17-β-estradiol for the Treatment of Neurodegenerative Diseases: an In Vitro and In Vivo Study</title><title>Pharmaceutical research</title><addtitle>Pharm Res</addtitle><addtitle>Pharm Res</addtitle><description>Purpose
Estradiol (E2)-loaded poly(L-lactide-co-glycolide-trimethylenecarbonate) (P(L-LA:GA:TMC)) rods with shape-memory were developed for the treatment of neurodegenerative diseases. Usefulness of the extrusion method in the obtaining process was also considered. The influence of structural and surface properties during hydrolytic degradation was developed. The possible therapeutic aspect of rods with E2 was determined.
Methods
The extruded rods were incubated in a PBS solution (pH 7.4, 37°C, 240 rpm). The amount of released E2
in vitro
conditions was estimated by UV-VIS method. The following methods in the degradation of rods were applied: NMR, DSC, FTIR, GPC, SEM, and optical microscopy. Changes in water uptake and weight loss were also determined.
In vivo
study was performed on rats. Measurements of E2 level were performed before and after ovariectomy of rats using ELISA method. A sample of tissue adjacent to the site of the rod implantation was analysed under an optical microscope.
Results
A stable and steady degradation process ensured zero-order release of E2. The
in vivo
study indicated a significant increase in the E2 level in serum after ovariectomy. Moreover, structural and surface features indicated that the extrusion method was appropriate for obtaining E2-loaded rods.
Conclusions
Shape-memory P(L-LA:GA:TMC) rods with E2 are an adequate proposal for further research in the field of neurological disorders.</description><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedicine</subject><subject>Drug Delivery Systems</subject><subject>Drug Liberation</subject><subject>Estradiol - administration & dosage</subject><subject>Estradiol - chemistry</subject><subject>Estradiol - pharmacokinetics</subject><subject>Female</subject><subject>Hydrolysis</subject><subject>Medical Law</subject><subject>Nanotubes - chemistry</subject><subject>Neurodegenerative Diseases - drug therapy</subject><subject>Pharmacology/Toxicology</subject><subject>Pharmacy</subject><subject>Polyesters - chemistry</subject><subject>Rats, Wistar</subject><subject>Research Paper</subject><subject>Surface Properties</subject><subject>Tissue Distribution</subject><issn>0724-8741</issn><issn>1573-904X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNqNkc9u1DAQxi0EokvhAbggH7kYPI4dOxyQUPlXqYBEF8TNcuPxbqokXuxk0d54Jh6EZ8JVSgUXxMljzTefZr4fIQ-BPwHO9dMMwBvFONRMcGhYc4usQOmKNVx-uU1WXAvJjJZwRO7lfMk5N9DIu-RI6FqYWqgV-X6-dTtk73CI6UDXmHaxPwyY6MfoM_3WTVsKmv38wTBPyfku9jTERKct0nVCNw04TjQG-h7nFD1ucMTkpm6P9GWX0WXMz6gb6elIP3dTiqX2y2cf6fk0-8N9cie4PuOD6_eYfHr9an3ylp19eHN68uKMtVKriXnRljs8xxoAWlAuXDSojEJnlDIyyBJFCy4EY8CZELgLXDtRVV5IqUJdHZPni-9uvhjQt2Xv5Hq7S93g0sFG19m_O2O3tZu4t4o3lQBeDB5fG6T4dS5x2KHLLfa9GzHO2YJRXJdQQf6HtKq1MMqYIoVF2qaYc8JwsxFwewXZLpBtgWyvINumzDz685Sbid9Ui0Asglxa4waTvYxzGku8_3D9Bd8PtE4</recordid><startdate>20161201</startdate><enddate>20161201</enddate><creator>Turek, Artur</creator><creator>Olakowska, Edyta</creator><creator>Borecka, Aleksandra</creator><creator>Janeczek, Henryk</creator><creator>Sobota, Michał</creator><creator>Jaworska, Joanna</creator><creator>Kaczmarczyk, Bożena</creator><creator>Jarząbek, Bożena</creator><creator>Gruchlik, Arkadiusz</creator><creator>Libera, Marcin</creator><creator>Liśkiewicz, Arkadiusz</creator><creator>Jędrzejowska-Szypułka, Halina</creator><creator>Kasperczyk, Janusz</creator><general>Springer US</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope><scope>5PM</scope></search><sort><creationdate>20161201</creationdate><title>Shape-Memory Terpolymer Rods with 17-β-estradiol for the Treatment of Neurodegenerative Diseases: an In Vitro and In Vivo Study</title><author>Turek, Artur ; Olakowska, Edyta ; Borecka, Aleksandra ; Janeczek, Henryk ; Sobota, Michał ; Jaworska, Joanna ; Kaczmarczyk, Bożena ; Jarząbek, Bożena ; Gruchlik, Arkadiusz ; Libera, Marcin ; Liśkiewicz, Arkadiusz ; Jędrzejowska-Szypułka, Halina ; Kasperczyk, Janusz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-d2c741d0e6111c15afb9e585ea85584f4110c1aff881a8ff0af07a233d2445f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biomedicine</topic><topic>Drug Delivery Systems</topic><topic>Drug Liberation</topic><topic>Estradiol - administration & dosage</topic><topic>Estradiol - chemistry</topic><topic>Estradiol - pharmacokinetics</topic><topic>Female</topic><topic>Hydrolysis</topic><topic>Medical Law</topic><topic>Nanotubes - chemistry</topic><topic>Neurodegenerative Diseases - drug therapy</topic><topic>Pharmacology/Toxicology</topic><topic>Pharmacy</topic><topic>Polyesters - chemistry</topic><topic>Rats, Wistar</topic><topic>Research Paper</topic><topic>Surface Properties</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Turek, Artur</creatorcontrib><creatorcontrib>Olakowska, Edyta</creatorcontrib><creatorcontrib>Borecka, Aleksandra</creatorcontrib><creatorcontrib>Janeczek, Henryk</creatorcontrib><creatorcontrib>Sobota, Michał</creatorcontrib><creatorcontrib>Jaworska, Joanna</creatorcontrib><creatorcontrib>Kaczmarczyk, Bożena</creatorcontrib><creatorcontrib>Jarząbek, Bożena</creatorcontrib><creatorcontrib>Gruchlik, Arkadiusz</creatorcontrib><creatorcontrib>Libera, Marcin</creatorcontrib><creatorcontrib>Liśkiewicz, Arkadiusz</creatorcontrib><creatorcontrib>Jędrzejowska-Szypułka, Halina</creatorcontrib><creatorcontrib>Kasperczyk, Janusz</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Pharmaceutical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Turek, Artur</au><au>Olakowska, Edyta</au><au>Borecka, Aleksandra</au><au>Janeczek, Henryk</au><au>Sobota, Michał</au><au>Jaworska, Joanna</au><au>Kaczmarczyk, Bożena</au><au>Jarząbek, Bożena</au><au>Gruchlik, Arkadiusz</au><au>Libera, Marcin</au><au>Liśkiewicz, Arkadiusz</au><au>Jędrzejowska-Szypułka, Halina</au><au>Kasperczyk, Janusz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Shape-Memory Terpolymer Rods with 17-β-estradiol for the Treatment of Neurodegenerative Diseases: an In Vitro and In Vivo Study</atitle><jtitle>Pharmaceutical research</jtitle><stitle>Pharm Res</stitle><addtitle>Pharm Res</addtitle><date>2016-12-01</date><risdate>2016</risdate><volume>33</volume><issue>12</issue><spage>2967</spage><epage>2978</epage><pages>2967-2978</pages><issn>0724-8741</issn><eissn>1573-904X</eissn><abstract>Purpose
Estradiol (E2)-loaded poly(L-lactide-co-glycolide-trimethylenecarbonate) (P(L-LA:GA:TMC)) rods with shape-memory were developed for the treatment of neurodegenerative diseases. Usefulness of the extrusion method in the obtaining process was also considered. The influence of structural and surface properties during hydrolytic degradation was developed. The possible therapeutic aspect of rods with E2 was determined.
Methods
The extruded rods were incubated in a PBS solution (pH 7.4, 37°C, 240 rpm). The amount of released E2
in vitro
conditions was estimated by UV-VIS method. The following methods in the degradation of rods were applied: NMR, DSC, FTIR, GPC, SEM, and optical microscopy. Changes in water uptake and weight loss were also determined.
In vivo
study was performed on rats. Measurements of E2 level were performed before and after ovariectomy of rats using ELISA method. A sample of tissue adjacent to the site of the rod implantation was analysed under an optical microscope.
Results
A stable and steady degradation process ensured zero-order release of E2. The
in vivo
study indicated a significant increase in the E2 level in serum after ovariectomy. Moreover, structural and surface features indicated that the extrusion method was appropriate for obtaining E2-loaded rods.
Conclusions
Shape-memory P(L-LA:GA:TMC) rods with E2 are an adequate proposal for further research in the field of neurological disorders.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>27628625</pmid><doi>10.1007/s11095-016-2019-9</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Pharmaceutical research, 2016-12, Vol.33 (12), p.2967-2978 |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Biochemistry Biomedical and Life Sciences Biomedical Engineering and Bioengineering Biomedicine Drug Delivery Systems Drug Liberation Estradiol - administration & dosage Estradiol - chemistry Estradiol - pharmacokinetics Female Hydrolysis Medical Law Nanotubes - chemistry Neurodegenerative Diseases - drug therapy Pharmacology/Toxicology Pharmacy Polyesters - chemistry Rats, Wistar Research Paper Surface Properties Tissue Distribution |
| title | Shape-Memory Terpolymer Rods with 17-β-estradiol for the Treatment of Neurodegenerative Diseases: an In Vitro and In Vivo Study |
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