Osteocytic connexin 43 is not required for the increase in bone mass induced by intermittent PTH administration in male mice
To investigate whether osteocytic connexin 43 (Cx43) is required for the bone response to intermittent PTH administration, and whether the connexin is involved in maintaining the bone matrix. Human PTH(1-34) was injected to adult male mice expressing (Cx43(fl/fl)) or not osteocytic Cx43 (Cx43(fl/fl)...
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| Veröffentlicht in: | Journal of musculoskeletal & neuronal interactions 2016-03, Vol.16 (1), p.45-57 |
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| creator | Pacheco-Costa, R Davis, H M Atkinson, E G Katchburian, E Plotkin, L I Reginato, R D |
| description | To investigate whether osteocytic connexin 43 (Cx43) is required for the bone response to intermittent PTH administration, and whether the connexin is involved in maintaining the bone matrix.
Human PTH(1-34) was injected to adult male mice expressing (Cx43(fl/fl)) or not osteocytic Cx43 (Cx43(fl/fl);DMP1-8kb-Cre) daily (100 µg/kg/d) for 14 days.
Cx43(fl/fl);DMP1-8kb-Cre mice have no difference in body weight and BMD from 1 to 4 months of age. Intermittent PTH administration increased BMD and BV/TV and induced a similar increase in type I collagen, alkaline phosphatase, runx2, osteocalcin, and bone sialoprotein expression in mice from both genotypes. On the other hand, osteocytic deletion of Cx43 did not alter mRNA levels of glycosaminoglycans, proteoglycans, collagens and osteoblast-related genes. In addition, expression of collagens assessed by immunohistochemistry was not affected by deleting osteocytic Cx43. However, PTH administration increased type II collagen only in Cx43(fl/fl) control mice, whereas hormone increased type I collagen expression only in Cx43(fl/fl);DMP1-8kb-Cre mice. Furthermore, PTH increased maturity of collagen fibers in control, but not in Cx43-deficient mice.
Expression of Cx43 in osteocytes is dispensable for bone anabolism induced by intermittent PTH administration; but it can modulate, at least in part, the effect of PTH on the bone matrix environment. |
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Human PTH(1-34) was injected to adult male mice expressing (Cx43(fl/fl)) or not osteocytic Cx43 (Cx43(fl/fl);DMP1-8kb-Cre) daily (100 µg/kg/d) for 14 days.
Cx43(fl/fl);DMP1-8kb-Cre mice have no difference in body weight and BMD from 1 to 4 months of age. Intermittent PTH administration increased BMD and BV/TV and induced a similar increase in type I collagen, alkaline phosphatase, runx2, osteocalcin, and bone sialoprotein expression in mice from both genotypes. On the other hand, osteocytic deletion of Cx43 did not alter mRNA levels of glycosaminoglycans, proteoglycans, collagens and osteoblast-related genes. In addition, expression of collagens assessed by immunohistochemistry was not affected by deleting osteocytic Cx43. However, PTH administration increased type II collagen only in Cx43(fl/fl) control mice, whereas hormone increased type I collagen expression only in Cx43(fl/fl);DMP1-8kb-Cre mice. Furthermore, PTH increased maturity of collagen fibers in control, but not in Cx43-deficient mice.
Expression of Cx43 in osteocytes is dispensable for bone anabolism induced by intermittent PTH administration; but it can modulate, at least in part, the effect of PTH on the bone matrix environment.</description><identifier>ISSN: 1108-7161</identifier><identifier>PMID: 26944823</identifier><language>eng</language><publisher>Greece: International Society of Musculoskeletal and Neuronal Interactions</publisher><subject>Absorptiometry, Photon ; Animals ; Bone and Bones - drug effects ; Bone and Bones - metabolism ; Bone Density - drug effects ; Connexin 43 - metabolism ; Humans ; Immunohistochemistry ; Male ; Mice ; Mice, Mutant Strains ; Original ; Osteoblasts - metabolism ; Osteogenesis - drug effects ; Parathyroid Hormone - pharmacology ; Reverse Transcriptase Polymerase Chain Reaction ; X-Ray Microtomography</subject><ispartof>Journal of musculoskeletal & neuronal interactions, 2016-03, Vol.16 (1), p.45-57</ispartof><rights>Copyright: © Journal of Musculoskeletal and Neuronal Interactions 2016</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5089455/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5089455/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26944823$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pacheco-Costa, R</creatorcontrib><creatorcontrib>Davis, H M</creatorcontrib><creatorcontrib>Atkinson, E G</creatorcontrib><creatorcontrib>Katchburian, E</creatorcontrib><creatorcontrib>Plotkin, L I</creatorcontrib><creatorcontrib>Reginato, R D</creatorcontrib><title>Osteocytic connexin 43 is not required for the increase in bone mass induced by intermittent PTH administration in male mice</title><title>Journal of musculoskeletal & neuronal interactions</title><addtitle>J Musculoskelet Neuronal Interact</addtitle><description>To investigate whether osteocytic connexin 43 (Cx43) is required for the bone response to intermittent PTH administration, and whether the connexin is involved in maintaining the bone matrix.
Human PTH(1-34) was injected to adult male mice expressing (Cx43(fl/fl)) or not osteocytic Cx43 (Cx43(fl/fl);DMP1-8kb-Cre) daily (100 µg/kg/d) for 14 days.
Cx43(fl/fl);DMP1-8kb-Cre mice have no difference in body weight and BMD from 1 to 4 months of age. Intermittent PTH administration increased BMD and BV/TV and induced a similar increase in type I collagen, alkaline phosphatase, runx2, osteocalcin, and bone sialoprotein expression in mice from both genotypes. On the other hand, osteocytic deletion of Cx43 did not alter mRNA levels of glycosaminoglycans, proteoglycans, collagens and osteoblast-related genes. In addition, expression of collagens assessed by immunohistochemistry was not affected by deleting osteocytic Cx43. However, PTH administration increased type II collagen only in Cx43(fl/fl) control mice, whereas hormone increased type I collagen expression only in Cx43(fl/fl);DMP1-8kb-Cre mice. Furthermore, PTH increased maturity of collagen fibers in control, but not in Cx43-deficient mice.
Expression of Cx43 in osteocytes is dispensable for bone anabolism induced by intermittent PTH administration; but it can modulate, at least in part, the effect of PTH on the bone matrix environment.</description><subject>Absorptiometry, Photon</subject><subject>Animals</subject><subject>Bone and Bones - drug effects</subject><subject>Bone and Bones - metabolism</subject><subject>Bone Density - drug effects</subject><subject>Connexin 43 - metabolism</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Mutant Strains</subject><subject>Original</subject><subject>Osteoblasts - metabolism</subject><subject>Osteogenesis - drug effects</subject><subject>Parathyroid Hormone - pharmacology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>X-Ray Microtomography</subject><issn>1108-7161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUEtLAzEQ3oNia_UvSI5eFpJsnhdBilqhUA_1vKTZWRvZTdokKxb88a5YRU_zDfO9mJNiSghWpSSCTIrzlF4xpoRRfFZMqNCMKVpNi49VyhDsITuLbPAe3p1HrEIuIR8yirAfXIQGtSGivAXkvI1g0hdAm-AB9SalcWkGO7I2hxFmiL3LGXxGT-sFMk3vvEs5muyC_9L1pht1zsJFcdqaLsHlcc6K5_u79XxRLlcPj_PbZbmjQuSSVYwzbInispEapNCqbQmWljKhONPAFQErMCZE2MYSwzTmSlPQuK2ksdWsuPn23Q2bHho7Voumq3fR9SYe6mBc_f_i3bZ-CW81x0ozzkeD66NBDPsBUq57lyx0nfEQhlQTKbFShGE9Uq_-Zv2G_Ly8-gSb_33d</recordid><startdate>201603</startdate><enddate>201603</enddate><creator>Pacheco-Costa, R</creator><creator>Davis, H M</creator><creator>Atkinson, E G</creator><creator>Katchburian, E</creator><creator>Plotkin, L I</creator><creator>Reginato, R D</creator><general>International Society of Musculoskeletal and Neuronal Interactions</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201603</creationdate><title>Osteocytic connexin 43 is not required for the increase in bone mass induced by intermittent PTH administration in male mice</title><author>Pacheco-Costa, R ; Davis, H M ; Atkinson, E G ; Katchburian, E ; Plotkin, L I ; Reginato, R D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p266t-434540c1857d79e7698ff107c2468549e581ec600116cdc1a4905892e90f37ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Absorptiometry, Photon</topic><topic>Animals</topic><topic>Bone and Bones - drug effects</topic><topic>Bone and Bones - metabolism</topic><topic>Bone Density - drug effects</topic><topic>Connexin 43 - metabolism</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Mutant Strains</topic><topic>Original</topic><topic>Osteoblasts - metabolism</topic><topic>Osteogenesis - drug effects</topic><topic>Parathyroid Hormone - pharmacology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>X-Ray Microtomography</topic><toplevel>online_resources</toplevel><creatorcontrib>Pacheco-Costa, R</creatorcontrib><creatorcontrib>Davis, H M</creatorcontrib><creatorcontrib>Atkinson, E G</creatorcontrib><creatorcontrib>Katchburian, E</creatorcontrib><creatorcontrib>Plotkin, L I</creatorcontrib><creatorcontrib>Reginato, R D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of musculoskeletal & neuronal interactions</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pacheco-Costa, R</au><au>Davis, H M</au><au>Atkinson, E G</au><au>Katchburian, E</au><au>Plotkin, L I</au><au>Reginato, R D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Osteocytic connexin 43 is not required for the increase in bone mass induced by intermittent PTH administration in male mice</atitle><jtitle>Journal of musculoskeletal & neuronal interactions</jtitle><addtitle>J Musculoskelet Neuronal Interact</addtitle><date>2016-03</date><risdate>2016</risdate><volume>16</volume><issue>1</issue><spage>45</spage><epage>57</epage><pages>45-57</pages><issn>1108-7161</issn><abstract>To investigate whether osteocytic connexin 43 (Cx43) is required for the bone response to intermittent PTH administration, and whether the connexin is involved in maintaining the bone matrix.
Human PTH(1-34) was injected to adult male mice expressing (Cx43(fl/fl)) or not osteocytic Cx43 (Cx43(fl/fl);DMP1-8kb-Cre) daily (100 µg/kg/d) for 14 days.
Cx43(fl/fl);DMP1-8kb-Cre mice have no difference in body weight and BMD from 1 to 4 months of age. Intermittent PTH administration increased BMD and BV/TV and induced a similar increase in type I collagen, alkaline phosphatase, runx2, osteocalcin, and bone sialoprotein expression in mice from both genotypes. On the other hand, osteocytic deletion of Cx43 did not alter mRNA levels of glycosaminoglycans, proteoglycans, collagens and osteoblast-related genes. In addition, expression of collagens assessed by immunohistochemistry was not affected by deleting osteocytic Cx43. However, PTH administration increased type II collagen only in Cx43(fl/fl) control mice, whereas hormone increased type I collagen expression only in Cx43(fl/fl);DMP1-8kb-Cre mice. Furthermore, PTH increased maturity of collagen fibers in control, but not in Cx43-deficient mice.
Expression of Cx43 in osteocytes is dispensable for bone anabolism induced by intermittent PTH administration; but it can modulate, at least in part, the effect of PTH on the bone matrix environment.</abstract><cop>Greece</cop><pub>International Society of Musculoskeletal and Neuronal Interactions</pub><pmid>26944823</pmid><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Absorptiometry, Photon Animals Bone and Bones - drug effects Bone and Bones - metabolism Bone Density - drug effects Connexin 43 - metabolism Humans Immunohistochemistry Male Mice Mice, Mutant Strains Original Osteoblasts - metabolism Osteogenesis - drug effects Parathyroid Hormone - pharmacology Reverse Transcriptase Polymerase Chain Reaction X-Ray Microtomography |
| title | Osteocytic connexin 43 is not required for the increase in bone mass induced by intermittent PTH administration in male mice |
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