Construction of a double congenic strain to prove an epistatic interaction on blood pressure between rat chromosomes 2 and 10
Previously we presented suggestive evidence from an F2 segregating population for an interaction on blood pressure (BP) between quantitative trait loci (QTL) on rat chromosomes (Chr) 2 and 10. To prove the existence of such an interaction, we developed congenic strains for Chr 2 and 10 by introgress...
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| Veröffentlicht in: | The Journal of clinical investigation 1998-04, Vol.101 (8), p.1591-1595 |
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| container_title | The Journal of clinical investigation |
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| creator | Rapp, J P Garrett, M R Deng, A Y |
| description | Previously we presented suggestive evidence from an F2 segregating population for an interaction on blood pressure (BP) between quantitative trait loci (QTL) on rat chromosomes (Chr) 2 and 10. To prove the existence of such an interaction, we developed congenic strains for Chr 2 and 10 by introgressing the low BP QTL alleles into the Dahl salt-sensitive (S) strain. A double congenic strain was also constructed with both the Chr 2 and 10 low BP QTL alleles on the S background. The four strains (S, Chr 2 congenic, Chr 10 congenic, and Chr 2/10 double congenic) were studied for BP response to increased salt intake. An analysis of variance showed significant main effects of Chr 2, Chr 10, and a significant interaction between Chr 2 and 10 on BP and heart weight (all P < 0.0001). The interaction accounted for 24 mmHg of BP and 79 mg of heart weight. Thus, the discovery and proof of epistatic interactions are clearly critical to understanding the genetics of blood pressure. |
| doi_str_mv | 10.1172/jci2251 |
| format | Article |
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To prove the existence of such an interaction, we developed congenic strains for Chr 2 and 10 by introgressing the low BP QTL alleles into the Dahl salt-sensitive (S) strain. A double congenic strain was also constructed with both the Chr 2 and 10 low BP QTL alleles on the S background. The four strains (S, Chr 2 congenic, Chr 10 congenic, and Chr 2/10 double congenic) were studied for BP response to increased salt intake. An analysis of variance showed significant main effects of Chr 2, Chr 10, and a significant interaction between Chr 2 and 10 on BP and heart weight (all P < 0.0001). The interaction accounted for 24 mmHg of BP and 79 mg of heart weight. Thus, the discovery and proof of epistatic interactions are clearly critical to understanding the genetics of blood pressure.</description><identifier>ISSN: 0021-9738</identifier><identifier>DOI: 10.1172/jci2251</identifier><identifier>PMID: 9541488</identifier><language>eng</language><publisher>United States</publisher><subject>Alleles ; Animals ; Blood Pressure - genetics ; Chromosome Mapping ; Crosses, Genetic ; Epistasis, Genetic ; Female ; Genetic Linkage ; Hypertension - genetics ; Hypertension - pathology ; Hypertension - physiopathology ; Male ; Myocardium - pathology ; Organ Size ; Quantitative Trait, Heritable ; Rats ; Rats, Inbred Strains ; Rats, Inbred WKY</subject><ispartof>The Journal of clinical investigation, 1998-04, Vol.101 (8), p.1591-1595</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-c453347379eea85bcf45b48bf78e3404c5c4b54fee71935b02aef0b01ebccfdb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC508739/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC508739/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9541488$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rapp, J P</creatorcontrib><creatorcontrib>Garrett, M R</creatorcontrib><creatorcontrib>Deng, A Y</creatorcontrib><title>Construction of a double congenic strain to prove an epistatic interaction on blood pressure between rat chromosomes 2 and 10</title><title>The Journal of clinical investigation</title><addtitle>J Clin Invest</addtitle><description>Previously we presented suggestive evidence from an F2 segregating population for an interaction on blood pressure (BP) between quantitative trait loci (QTL) on rat chromosomes (Chr) 2 and 10. To prove the existence of such an interaction, we developed congenic strains for Chr 2 and 10 by introgressing the low BP QTL alleles into the Dahl salt-sensitive (S) strain. A double congenic strain was also constructed with both the Chr 2 and 10 low BP QTL alleles on the S background. The four strains (S, Chr 2 congenic, Chr 10 congenic, and Chr 2/10 double congenic) were studied for BP response to increased salt intake. An analysis of variance showed significant main effects of Chr 2, Chr 10, and a significant interaction between Chr 2 and 10 on BP and heart weight (all P < 0.0001). The interaction accounted for 24 mmHg of BP and 79 mg of heart weight. Thus, the discovery and proof of epistatic interactions are clearly critical to understanding the genetics of blood pressure.</description><subject>Alleles</subject><subject>Animals</subject><subject>Blood Pressure - genetics</subject><subject>Chromosome Mapping</subject><subject>Crosses, Genetic</subject><subject>Epistasis, Genetic</subject><subject>Female</subject><subject>Genetic Linkage</subject><subject>Hypertension - genetics</subject><subject>Hypertension - pathology</subject><subject>Hypertension - physiopathology</subject><subject>Male</subject><subject>Myocardium - pathology</subject><subject>Organ Size</subject><subject>Quantitative Trait, Heritable</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Rats, Inbred WKY</subject><issn>0021-9738</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkT1vHCEYhCkSOY5j5RdEonKqs2GBwBYuopM_ZSlNXCNg37WxdnkvwNpykf9uTj5ZTpUGinlmNDCEfOXsmHPdnTyE2HWKfyD7jHV81WthPpHPpTwwxqVUco_s9Upyacw--bvGVGpeQo2YKI7U0QEXPwENmO4gxUCb7GKiFekm4yNQlyhsYqmuNjGmCtnt3In6CXFoHJSyZKAe6hNAotlVGu4zzlhwhkK7FjJQzr6Qj6ObChzu7gNye372e325uvl1cbX-ebMK8ger7VRCSC10D-CM8mGUykvjR21ASCaDCtIrOQJo3gvlWedgZJ5x8CGMgxcH5PQ1d7P4GYYAqb1pspscZ5efLbpo_1VSvLd3-GgVM1r0zX-082f8s0Cpdo4lwDS5BLgUq3vDFGsl_wdyzU37-m3i91cwZCwlw_hWhjO7XdFer6-2Kzby2_vub9xuQvECtxidPA</recordid><startdate>19980415</startdate><enddate>19980415</enddate><creator>Rapp, J P</creator><creator>Garrett, M R</creator><creator>Deng, A Y</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19980415</creationdate><title>Construction of a double congenic strain to prove an epistatic interaction on blood pressure between rat chromosomes 2 and 10</title><author>Rapp, J P ; Garrett, M R ; Deng, A Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-c453347379eea85bcf45b48bf78e3404c5c4b54fee71935b02aef0b01ebccfdb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Alleles</topic><topic>Animals</topic><topic>Blood Pressure - genetics</topic><topic>Chromosome Mapping</topic><topic>Crosses, Genetic</topic><topic>Epistasis, Genetic</topic><topic>Female</topic><topic>Genetic Linkage</topic><topic>Hypertension - genetics</topic><topic>Hypertension - pathology</topic><topic>Hypertension - physiopathology</topic><topic>Male</topic><topic>Myocardium - pathology</topic><topic>Organ Size</topic><topic>Quantitative Trait, Heritable</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Rats, Inbred WKY</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rapp, J P</creatorcontrib><creatorcontrib>Garrett, M R</creatorcontrib><creatorcontrib>Deng, A Y</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of clinical investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rapp, J P</au><au>Garrett, M R</au><au>Deng, A Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of a double congenic strain to prove an epistatic interaction on blood pressure between rat chromosomes 2 and 10</atitle><jtitle>The Journal of clinical investigation</jtitle><addtitle>J Clin Invest</addtitle><date>1998-04-15</date><risdate>1998</risdate><volume>101</volume><issue>8</issue><spage>1591</spage><epage>1595</epage><pages>1591-1595</pages><issn>0021-9738</issn><abstract>Previously we presented suggestive evidence from an F2 segregating population for an interaction on blood pressure (BP) between quantitative trait loci (QTL) on rat chromosomes (Chr) 2 and 10. To prove the existence of such an interaction, we developed congenic strains for Chr 2 and 10 by introgressing the low BP QTL alleles into the Dahl salt-sensitive (S) strain. A double congenic strain was also constructed with both the Chr 2 and 10 low BP QTL alleles on the S background. The four strains (S, Chr 2 congenic, Chr 10 congenic, and Chr 2/10 double congenic) were studied for BP response to increased salt intake. An analysis of variance showed significant main effects of Chr 2, Chr 10, and a significant interaction between Chr 2 and 10 on BP and heart weight (all P < 0.0001). The interaction accounted for 24 mmHg of BP and 79 mg of heart weight. Thus, the discovery and proof of epistatic interactions are clearly critical to understanding the genetics of blood pressure.</abstract><cop>United States</cop><pmid>9541488</pmid><doi>10.1172/jci2251</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Alleles Animals Blood Pressure - genetics Chromosome Mapping Crosses, Genetic Epistasis, Genetic Female Genetic Linkage Hypertension - genetics Hypertension - pathology Hypertension - physiopathology Male Myocardium - pathology Organ Size Quantitative Trait, Heritable Rats Rats, Inbred Strains Rats, Inbred WKY |
| title | Construction of a double congenic strain to prove an epistatic interaction on blood pressure between rat chromosomes 2 and 10 |
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