Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology
The delayed reporting of antimicrobial susceptibility testing remains a limiting factor in clinical decision-making in the treatment of bacterial infection. This study evaluates the use of forward laser light scatter (FLLS) to measure bacterial growth for the early determination of antimicrobial sus...
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| Veröffentlicht in: | Journal of clinical microbiology 2016-11, Vol.54 (11), p.2701-2706 |
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| container_issue | 11 |
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| container_title | Journal of clinical microbiology |
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| creator | Hayden, Randall T Clinton, Lani K Hewitt, Carolyn Koyamatsu, Terri Sun, Yilun Jamison, Ginger Perkins, Rosalie Tang, Li Pounds, Stanley Bankowski, Matthew J |
| description | The delayed reporting of antimicrobial susceptibility testing remains a limiting factor in clinical decision-making in the treatment of bacterial infection. This study evaluates the use of forward laser light scatter (FLLS) to measure bacterial growth for the early determination of antimicrobial susceptibility. Three isolates each (two clinical isolates and one reference strain) of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were tested in triplicate using two commercial antimicrobial testing systems, the Vitek2 and the MicroScan MIC panel, to challenge the BacterioScan FLLS. The BacterioScan FLLS showed a high degree of categorical concordance with the commercial methods. Pairwise comparison with each commercial system serving as a reference standard showed 88.9% agreement with MicroScan (two minor errors) and 72.2% agreement with Vitek (five minor errors). FLLS using the BacterioScan system shows promise as a novel method for the rapid and accurate determination of antimicrobial susceptibility. |
| doi_str_mv | 10.1128/JCM.01475-16 |
| format | Article |
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This study evaluates the use of forward laser light scatter (FLLS) to measure bacterial growth for the early determination of antimicrobial susceptibility. Three isolates each (two clinical isolates and one reference strain) of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were tested in triplicate using two commercial antimicrobial testing systems, the Vitek2 and the MicroScan MIC panel, to challenge the BacterioScan FLLS. The BacterioScan FLLS showed a high degree of categorical concordance with the commercial methods. Pairwise comparison with each commercial system serving as a reference standard showed 88.9% agreement with MicroScan (two minor errors) and 72.2% agreement with Vitek (five minor errors). FLLS using the BacterioScan system shows promise as a novel method for the rapid and accurate determination of antimicrobial susceptibility.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/JCM.01475-16</identifier><identifier>PMID: 27558176</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Anti-Bacterial Agents - pharmacology ; Bacteriology ; Dynamic Light Scattering - methods ; Escherichia coli ; Escherichia coli - drug effects ; Escherichia coli - growth & development ; Humans ; Lasers ; Microbial Sensitivity Tests - methods ; Pilot Projects ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - drug effects ; Pseudomonas aeruginosa - growth & development ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - growth & development ; Time Factors</subject><ispartof>Journal of clinical microbiology, 2016-11, Vol.54 (11), p.2701-2706</ispartof><rights>Copyright © 2016, American Society for Microbiology. All Rights Reserved.</rights><rights>Copyright © 2016, American Society for Microbiology. All Rights Reserved. 2016 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-76b7e7c83e80db68c348b0aa389bc2e1f9fbd340f0c6e64cdb43ff99e7ecb0d23</citedby><cites>FETCH-LOGICAL-c417t-76b7e7c83e80db68c348b0aa389bc2e1f9fbd340f0c6e64cdb43ff99e7ecb0d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5078546/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5078546/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27558176$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Patel, R.</contributor><creatorcontrib>Hayden, Randall T</creatorcontrib><creatorcontrib>Clinton, Lani K</creatorcontrib><creatorcontrib>Hewitt, Carolyn</creatorcontrib><creatorcontrib>Koyamatsu, Terri</creatorcontrib><creatorcontrib>Sun, Yilun</creatorcontrib><creatorcontrib>Jamison, Ginger</creatorcontrib><creatorcontrib>Perkins, Rosalie</creatorcontrib><creatorcontrib>Tang, Li</creatorcontrib><creatorcontrib>Pounds, Stanley</creatorcontrib><creatorcontrib>Bankowski, Matthew J</creatorcontrib><title>Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology</title><title>Journal of clinical microbiology</title><addtitle>J Clin Microbiol</addtitle><description>The delayed reporting of antimicrobial susceptibility testing remains a limiting factor in clinical decision-making in the treatment of bacterial infection. This study evaluates the use of forward laser light scatter (FLLS) to measure bacterial growth for the early determination of antimicrobial susceptibility. Three isolates each (two clinical isolates and one reference strain) of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were tested in triplicate using two commercial antimicrobial testing systems, the Vitek2 and the MicroScan MIC panel, to challenge the BacterioScan FLLS. The BacterioScan FLLS showed a high degree of categorical concordance with the commercial methods. Pairwise comparison with each commercial system serving as a reference standard showed 88.9% agreement with MicroScan (two minor errors) and 72.2% agreement with Vitek (five minor errors). FLLS using the BacterioScan system shows promise as a novel method for the rapid and accurate determination of antimicrobial susceptibility.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacteriology</subject><subject>Dynamic Light Scattering - methods</subject><subject>Escherichia coli</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - growth & development</subject><subject>Humans</subject><subject>Lasers</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>Pilot Projects</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - drug effects</subject><subject>Pseudomonas aeruginosa - growth & development</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - growth & development</subject><subject>Time Factors</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1PwyAchonRuDm9eTY9erATSgv0YrIszo_MmLgteiNA6Ybp2glMs_9e6qbRmxcI8OTN7-UB4BTBPkIJu7wfPvQhSmkWI7IHugjmLCYEvuyDLoR5uEWYdsCRc68wYGmWHYJOQrOMIUq64PlJrEwRDWpvlkbZRhpRRZO1U3rljTSV8Ztoqp039TyauXYdNfZD2CIaC6dtNDbzhY8mSngfTlOtFnVTNfPNMTgoReX0yW7vgdnoejq8jcePN3fDwThWKaI-pkRSTRXDmsFCEqZwyiQUArNcqkSjMi9lgVNYQkU0SVUhU1yWea6pVhIWCe6Bq23uai2XulC69lZUfGXNUtgNb4Thf19qs-Dz5p1nkLIsJSHgfBdgm7d1aMqXJrSvKlHrZu04YjRhSRLIf6A4R62JFr3YouFLnbO6_JkIQd5q40Eb_9LGUTvE2e8WP_C3J_wJpv6VSg</recordid><startdate>20161101</startdate><enddate>20161101</enddate><creator>Hayden, Randall T</creator><creator>Clinton, Lani K</creator><creator>Hewitt, Carolyn</creator><creator>Koyamatsu, Terri</creator><creator>Sun, Yilun</creator><creator>Jamison, Ginger</creator><creator>Perkins, Rosalie</creator><creator>Tang, Li</creator><creator>Pounds, Stanley</creator><creator>Bankowski, Matthew J</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope><scope>5PM</scope></search><sort><creationdate>20161101</creationdate><title>Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology</title><author>Hayden, Randall T ; Clinton, Lani K ; Hewitt, Carolyn ; Koyamatsu, Terri ; Sun, Yilun ; Jamison, Ginger ; Perkins, Rosalie ; Tang, Li ; Pounds, Stanley ; Bankowski, Matthew J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-76b7e7c83e80db68c348b0aa389bc2e1f9fbd340f0c6e64cdb43ff99e7ecb0d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacteriology</topic><topic>Dynamic Light Scattering - methods</topic><topic>Escherichia coli</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - growth & development</topic><topic>Humans</topic><topic>Lasers</topic><topic>Microbial Sensitivity Tests - methods</topic><topic>Pilot Projects</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - drug effects</topic><topic>Pseudomonas aeruginosa - growth & development</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - growth & development</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hayden, Randall T</creatorcontrib><creatorcontrib>Clinton, Lani K</creatorcontrib><creatorcontrib>Hewitt, Carolyn</creatorcontrib><creatorcontrib>Koyamatsu, Terri</creatorcontrib><creatorcontrib>Sun, Yilun</creatorcontrib><creatorcontrib>Jamison, Ginger</creatorcontrib><creatorcontrib>Perkins, Rosalie</creatorcontrib><creatorcontrib>Tang, Li</creatorcontrib><creatorcontrib>Pounds, Stanley</creatorcontrib><creatorcontrib>Bankowski, Matthew J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hayden, Randall T</au><au>Clinton, Lani K</au><au>Hewitt, Carolyn</au><au>Koyamatsu, Terri</au><au>Sun, Yilun</au><au>Jamison, Ginger</au><au>Perkins, Rosalie</au><au>Tang, Li</au><au>Pounds, Stanley</au><au>Bankowski, Matthew J</au><au>Patel, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology</atitle><jtitle>Journal of clinical microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>2016-11-01</date><risdate>2016</risdate><volume>54</volume><issue>11</issue><spage>2701</spage><epage>2706</epage><pages>2701-2706</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><abstract>The delayed reporting of antimicrobial susceptibility testing remains a limiting factor in clinical decision-making in the treatment of bacterial infection. This study evaluates the use of forward laser light scatter (FLLS) to measure bacterial growth for the early determination of antimicrobial susceptibility. Three isolates each (two clinical isolates and one reference strain) of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were tested in triplicate using two commercial antimicrobial testing systems, the Vitek2 and the MicroScan MIC panel, to challenge the BacterioScan FLLS. The BacterioScan FLLS showed a high degree of categorical concordance with the commercial methods. Pairwise comparison with each commercial system serving as a reference standard showed 88.9% agreement with MicroScan (two minor errors) and 72.2% agreement with Vitek (five minor errors). 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| source | American Society for Microbiology; MEDLINE; PubMed Central; EZB Electronic Journals Library |
| subjects | Anti-Bacterial Agents - pharmacology Bacteriology Dynamic Light Scattering - methods Escherichia coli Escherichia coli - drug effects Escherichia coli - growth & development Humans Lasers Microbial Sensitivity Tests - methods Pilot Projects Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - growth & development Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - growth & development Time Factors |
| title | Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology |
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