Minocycline Effects on IL-6 Concentration in Macrophage and Microglial Cells in a Rat Model of Neuropathic Pain
Evidence indicates that neuropathic pain pathogenesis is not confined to changes in the activity of neuronal systems but involves interactions between neurons, inflammatory immune and immune-like glial cells. Substances released from immune cells during inflammation play an important role in develop...
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| Veröffentlicht in: | Iranian biomedical journal 2016-11, Vol.20 (5), p.273-279 |
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| creator | Moini-Zanjani, Taraneh Ostad, Seyed-Nasser Labibi, Farzaneh Ameli, Haleh Mosaffa, Nariman Sabetkasaei, Masoumeh |
| description | Evidence indicates that neuropathic pain pathogenesis is not confined to changes in the activity of neuronal systems but involves interactions between neurons, inflammatory immune and immune-like glial cells. Substances released from immune cells during inflammation play an important role in development and maintenance of neuropathic pain. It has been found that minocycline suppresses the development of neuropathic pain. Here, we evaluated the analgesic effect of minocycline in a chronic constriction injury (CCI) model of neuropathic pain in rat and assessed IL-6 concentration from cultured macrophage and microglia cells.
Male Wistar rat (n=6, 150-200 g) were divided into three different groups: 1) CCI+vehicle, 2) sham+vehicle, and 3) CCI+drug. Minocycline (10, 20, and 40 mg/kg) was injected one hour before surgery and continued daily to day 14 post ligation. Von Frey filaments and acetone, as pain behavioral tests, were used for mechanical allodynia and cold allodynia, respectively. Experiments were performed on day 0 (before surgery) and days 1, 3, 5, 7, 10, and 14 post -injury. At day 14, rats were killed and monocyte-derived macrophage from right ventricle and microglia from lumbar part of the spinal cord were isolated and cultured in RPMI and Leibovitz's media, respectively. IL-6 concentration was evaluated in cell culture supernatant after 24 h.
Minocycline (10, 20, and 40 mg/kg) attenuated pain behavior, and a decrease in IL-6 concentration was observed in immune cells compared to CCI vehicle-treated animals.
Minocycline reduced pain behavior and decreased IL-6 concentration in macrophage and microglial cells. |
| doi_str_mv | 10.22045/ibj.2016.04 |
| format | Article |
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Male Wistar rat (n=6, 150-200 g) were divided into three different groups: 1) CCI+vehicle, 2) sham+vehicle, and 3) CCI+drug. Minocycline (10, 20, and 40 mg/kg) was injected one hour before surgery and continued daily to day 14 post ligation. Von Frey filaments and acetone, as pain behavioral tests, were used for mechanical allodynia and cold allodynia, respectively. Experiments were performed on day 0 (before surgery) and days 1, 3, 5, 7, 10, and 14 post -injury. At day 14, rats were killed and monocyte-derived macrophage from right ventricle and microglia from lumbar part of the spinal cord were isolated and cultured in RPMI and Leibovitz's media, respectively. IL-6 concentration was evaluated in cell culture supernatant after 24 h.
Minocycline (10, 20, and 40 mg/kg) attenuated pain behavior, and a decrease in IL-6 concentration was observed in immune cells compared to CCI vehicle-treated animals.
Minocycline reduced pain behavior and decreased IL-6 concentration in macrophage and microglial cells.</description><identifier>ISSN: 1028-852X</identifier><identifier>EISSN: 2008-823X</identifier><identifier>DOI: 10.22045/ibj.2016.04</identifier><identifier>PMID: 27221523</identifier><language>eng</language><publisher>Iran: Pasteur Institute of Iran</publisher><subject>Analgesics ; Analgesics - therapeutic use ; Animals ; Cell culture ; Cytokines ; Disease Models, Animal ; Full Lenght ; Hyperalgesia - drug therapy ; Interleukin-6 - metabolism ; Macrophages - metabolism ; Male ; Microglia - metabolism ; Minocycline - therapeutic use ; Neuralgia - drug therapy ; Neuralgia - pathology ; Neuroglia - pathology ; Neurons - pathology ; Ostomy ; Pain ; Pathogenesis ; Rats ; Rats, Wistar ; Rodents ; Spinal cord ; Studies ; Surgery ; Tumor necrosis factor-TNF</subject><ispartof>Iranian biomedical journal, 2016-11, Vol.20 (5), p.273-279</ispartof><rights>Copyright Pasteur Institute of Iran Nov 2016</rights><rights>Copyright: © Iranian Biomedical Journal 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c285t-a82bcb644e93fdd231d8ba4d7784478c33e629a776edcd7fcf4908b63f7d9d153</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5075140/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5075140/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27221523$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moini-Zanjani, Taraneh</creatorcontrib><creatorcontrib>Ostad, Seyed-Nasser</creatorcontrib><creatorcontrib>Labibi, Farzaneh</creatorcontrib><creatorcontrib>Ameli, Haleh</creatorcontrib><creatorcontrib>Mosaffa, Nariman</creatorcontrib><creatorcontrib>Sabetkasaei, Masoumeh</creatorcontrib><title>Minocycline Effects on IL-6 Concentration in Macrophage and Microglial Cells in a Rat Model of Neuropathic Pain</title><title>Iranian biomedical journal</title><addtitle>Iran Biomed J</addtitle><description>Evidence indicates that neuropathic pain pathogenesis is not confined to changes in the activity of neuronal systems but involves interactions between neurons, inflammatory immune and immune-like glial cells. Substances released from immune cells during inflammation play an important role in development and maintenance of neuropathic pain. It has been found that minocycline suppresses the development of neuropathic pain. Here, we evaluated the analgesic effect of minocycline in a chronic constriction injury (CCI) model of neuropathic pain in rat and assessed IL-6 concentration from cultured macrophage and microglia cells.
Male Wistar rat (n=6, 150-200 g) were divided into three different groups: 1) CCI+vehicle, 2) sham+vehicle, and 3) CCI+drug. Minocycline (10, 20, and 40 mg/kg) was injected one hour before surgery and continued daily to day 14 post ligation. Von Frey filaments and acetone, as pain behavioral tests, were used for mechanical allodynia and cold allodynia, respectively. Experiments were performed on day 0 (before surgery) and days 1, 3, 5, 7, 10, and 14 post -injury. At day 14, rats were killed and monocyte-derived macrophage from right ventricle and microglia from lumbar part of the spinal cord were isolated and cultured in RPMI and Leibovitz's media, respectively. IL-6 concentration was evaluated in cell culture supernatant after 24 h.
Minocycline (10, 20, and 40 mg/kg) attenuated pain behavior, and a decrease in IL-6 concentration was observed in immune cells compared to CCI vehicle-treated animals.
Minocycline reduced pain behavior and decreased IL-6 concentration in macrophage and microglial cells.</description><subject>Analgesics</subject><subject>Analgesics - therapeutic use</subject><subject>Animals</subject><subject>Cell culture</subject><subject>Cytokines</subject><subject>Disease Models, Animal</subject><subject>Full Lenght</subject><subject>Hyperalgesia - drug therapy</subject><subject>Interleukin-6 - metabolism</subject><subject>Macrophages - metabolism</subject><subject>Male</subject><subject>Microglia - metabolism</subject><subject>Minocycline - therapeutic use</subject><subject>Neuralgia - drug therapy</subject><subject>Neuralgia - pathology</subject><subject>Neuroglia - pathology</subject><subject>Neurons - pathology</subject><subject>Ostomy</subject><subject>Pain</subject><subject>Pathogenesis</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Rodents</subject><subject>Spinal cord</subject><subject>Studies</subject><subject>Surgery</subject><subject>Tumor necrosis factor-TNF</subject><issn>1028-852X</issn><issn>2008-823X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqNkctPGzEQh62KqgTorWdkiQuXTe3xcy9IKKIQKYGqohK3ldf2Jo42dtgHUv77uoWilhOneX3z0zwQ-kLJFIBw8TXUmykQKqeEf0ATIEQXGtjDAZpQAtkX8HCIjvp-QwgTVKlP6BAUABXAJigtQ0x2b9sQPb5qGm-HHqeI54tC4lmK1sehM0PIqRDx0tgu7dZm5bGJDi9DDldtMC2e-bbtfyMG_zADXibnW5wafOvH3GGGdbD4uwnxBH1sTNv7zy_2GP38dnU_uykWd9fz2eWisKDFUBgNta0l575kjXPAqNO14U4pzbnSljEvoTRKSe-sU41teEl0LVmjXOmoYMfo4ll3N9bbzPxZo612Xdiabl8lE6r_KzGsq1V6qgRRgnKSBc5fBLr0OPp-qLaht3lLE30a-4rqTGpWgngHClTpUmqZ0bM36CaNXcyXyBSTpRSlhEyd_jv869R_38Z-AbMymYU</recordid><startdate>201611</startdate><enddate>201611</enddate><creator>Moini-Zanjani, Taraneh</creator><creator>Ostad, Seyed-Nasser</creator><creator>Labibi, Farzaneh</creator><creator>Ameli, Haleh</creator><creator>Mosaffa, Nariman</creator><creator>Sabetkasaei, Masoumeh</creator><general>Pasteur Institute of Iran</general><general>Pasteur Institute</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QO</scope><scope>7T5</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201611</creationdate><title>Minocycline Effects on IL-6 Concentration in Macrophage and Microglial Cells in a Rat Model of Neuropathic Pain</title><author>Moini-Zanjani, Taraneh ; 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Substances released from immune cells during inflammation play an important role in development and maintenance of neuropathic pain. It has been found that minocycline suppresses the development of neuropathic pain. Here, we evaluated the analgesic effect of minocycline in a chronic constriction injury (CCI) model of neuropathic pain in rat and assessed IL-6 concentration from cultured macrophage and microglia cells.
Male Wistar rat (n=6, 150-200 g) were divided into three different groups: 1) CCI+vehicle, 2) sham+vehicle, and 3) CCI+drug. Minocycline (10, 20, and 40 mg/kg) was injected one hour before surgery and continued daily to day 14 post ligation. Von Frey filaments and acetone, as pain behavioral tests, were used for mechanical allodynia and cold allodynia, respectively. Experiments were performed on day 0 (before surgery) and days 1, 3, 5, 7, 10, and 14 post -injury. At day 14, rats were killed and monocyte-derived macrophage from right ventricle and microglia from lumbar part of the spinal cord were isolated and cultured in RPMI and Leibovitz's media, respectively. IL-6 concentration was evaluated in cell culture supernatant after 24 h.
Minocycline (10, 20, and 40 mg/kg) attenuated pain behavior, and a decrease in IL-6 concentration was observed in immune cells compared to CCI vehicle-treated animals.
Minocycline reduced pain behavior and decreased IL-6 concentration in macrophage and microglial cells.</abstract><cop>Iran</cop><pub>Pasteur Institute of Iran</pub><pmid>27221523</pmid><doi>10.22045/ibj.2016.04</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | PubMed (Medline); MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Analgesics Analgesics - therapeutic use Animals Cell culture Cytokines Disease Models, Animal Full Lenght Hyperalgesia - drug therapy Interleukin-6 - metabolism Macrophages - metabolism Male Microglia - metabolism Minocycline - therapeutic use Neuralgia - drug therapy Neuralgia - pathology Neuroglia - pathology Neurons - pathology Ostomy Pain Pathogenesis Rats Rats, Wistar Rodents Spinal cord Studies Surgery Tumor necrosis factor-TNF |
| title | Minocycline Effects on IL-6 Concentration in Macrophage and Microglial Cells in a Rat Model of Neuropathic Pain |
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