Used protocols for isolation and propagation of ovarian stem cells, different cells with different traits
Although existence of ovarian stem cells (OSCs) in mammalian postnatal ovary is still under controversy, however, it has been almost accepted that OSCs are contributing actively to folliculogenesis and neo-oogenesis. Recently, various methods with different efficacies have been employed for OSCs iso...
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Veröffentlicht in: | Journal of ovarian research 2016-10, Vol.9 (1), p.68-68, Article 68 |
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creator | Yazdekhasti, Hossein Rajabi, Zahra Parvari, Soraya Abbasi, Mehdi |
description | Although existence of ovarian stem cells (OSCs) in mammalian postnatal ovary is still under controversy, however, it has been almost accepted that OSCs are contributing actively to folliculogenesis and neo-oogenesis. Recently, various methods with different efficacies have been employed for OSCs isolation from ovarian tissue, which these methods could be chosen depends on aim of isolation and accessible equipments and materials in lab. Although isolated OSCs from different methods have various traits and characterizations, which might become from their different nature and origin, however these stem cells are promising source for woman infertility treatment or source of energy for women with a history of repeat IVF failure in near future. This review has brought together and summarized currently used protocols for isolation and propagation of OSCs in vitro. |
doi_str_mv | 10.1186/s13048-016-0274-3 |
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subjects | Analysis Animals Care and treatment Cell Differentiation Cell Separation - methods Diagnosis Female Flow Cytometry Humans Immunomagnetic Separation Mice, Transgenic Oocytes - cytology Oocytes - metabolism Oogenesis Ovarian diseases Ovary - cytology Phenotype Review Stem cells |
title | Used protocols for isolation and propagation of ovarian stem cells, different cells with different traits |
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