Candidate Gene Expression in Bos indicus Ovarian Tissues: Prepubertal and Postpubertal Heifers in Diestrus
Growth factors such as bone morphogenetic proteins 6, 7, 15, and two isoforms of transforming growth factor-beta (BMP6, BMP7, BMP15, TGFB1, and TGFB2), and insulin-like growth factor system act as local regulators of ovarian follicular development. To elucidate if these factors as well as others can...
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| Veröffentlicht in: | Frontiers in veterinary science 2016-10, Vol.3, p.94-94 |
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| creator | Weller, Mayara Morena Del Cambre Amaral Fortes, Marina Rufino S Porto-Neto, Laercio R Kelly, Matthew Venus, Bronwyn Kidd, Lisa do Rego, João Paulo Arcelino Edwards, Sophia Boe-Hansen, Gry B Piper, Emily Lehnert, Sigrid A Guimarães, Simone Eliza Facioni Moore, Stephen Stewart |
| description | Growth factors such as bone morphogenetic proteins 6, 7, 15, and two isoforms of transforming growth factor-beta (BMP6, BMP7, BMP15, TGFB1, and TGFB2), and insulin-like growth factor system act as local regulators of ovarian follicular development. To elucidate if these factors as well as others candidate genes, such as estrogen receptor 1 (
), growth differentiation factor 9 (
), follicle-stimulating hormone receptor (
), luteinizing hormone receptor (
), bone morphogenetic protein receptor, type 2 (
), type 1 insulin-like growth factor receptor (
), and key steroidogenic enzymes cytochrome P450 aromatase and 3-β-hydroxysteroid dehydrogenase (
and
) could modulate or influence diestrus on the onset of puberty in Brahman heifers, their ovarian mRNA expression was measured before and after puberty (luteal phase). Six postpubertal (POST) heifers were euthanized on the luteal phase of their second cycle, confirmed by corpus luteum observation, and six prepubertal (PRE) heifers were euthanized in the same day. Quantitative real-time PCR analysis showed that the expression of
, and
mRNA was greater in PRE heifers, when contrasted to POST heifers. The expression of
and
was lower in PRE heifers. Differential expression of ovarian genes could be associated with changes in follicular dynamics and different cell populations that have emerged as consequence of puberty and the luteal phase. The emerging hypothesis is that
and
are co-expressed and may modulate the expression of
and
, and
.
could influence the downregulation of
and upregulation of
and
, which mediates the follicular dynamics in heifer ovaries. Upregulation of
expression prepuberty, compared to postpuberty diestrus, correlates with increased levels
and
. Thus,
and
may play synergic roles and were predicted to interact, from the expression data (
= 0.07,
= 0.84). The role of these co-expressed genes in puberty and heifers luteal phase merits further research. |
| doi_str_mv | 10.3389/fvets.2016.00094 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5067528</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1835528778</sourcerecordid><originalsourceid>FETCH-LOGICAL-c396t-1704a7922d2d093032919274c4930d0f14973858afe5d3ed3090055f3aec72e93</originalsourceid><addsrcrecordid>eNpVUT1PwzAQtRCIosLOhDyytFzspI4ZkKCUFqlSGWC2THwBozQpvqSCf4_LR1Wmu7Pfe_dOj7HTBIZS5vqiXGNLQwHJaAgAOt1jR0JoNUjUSO_v9D12QvQWIUmWKpnDIesJlUOUyI_Y29jWzjvbIp9ijXzysQpI5Jua-5rfNBSL80VHfLG2wduaP3qiDumSPwRcdc8YWlvxKMIfGmq3DzP0JYYNm996pDZ0dMwOSlsRnvzWPnu6mzyOZ4P5Yno_vp4PCqlHbbQMqVVaCCccaAlS6EQLlRZpHByUSarjFVluS8ycRCdBA2RZKS0WSqCWfXb1oxvNLNEVWLfBVmYV_NKGT9NYb_7_1P7VvDRrk8FIZSKPAue_AqF5j6e2ZumpwKqyNTYdmSSXWcQptYHCD7QIDVHAcrsmAbNJyXynZDYpme-UIuVs196W8JeJ_AL3TY_e</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1835528778</pqid></control><display><type>article</type><title>Candidate Gene Expression in Bos indicus Ovarian Tissues: Prepubertal and Postpubertal Heifers in Diestrus</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>PubMed Central</source><creator>Weller, Mayara Morena Del Cambre Amaral ; Fortes, Marina Rufino S ; Porto-Neto, Laercio R ; Kelly, Matthew ; Venus, Bronwyn ; Kidd, Lisa ; do Rego, João Paulo Arcelino ; Edwards, Sophia ; Boe-Hansen, Gry B ; Piper, Emily ; Lehnert, Sigrid A ; Guimarães, Simone Eliza Facioni ; Moore, Stephen Stewart</creator><creatorcontrib>Weller, Mayara Morena Del Cambre Amaral ; Fortes, Marina Rufino S ; Porto-Neto, Laercio R ; Kelly, Matthew ; Venus, Bronwyn ; Kidd, Lisa ; do Rego, João Paulo Arcelino ; Edwards, Sophia ; Boe-Hansen, Gry B ; Piper, Emily ; Lehnert, Sigrid A ; Guimarães, Simone Eliza Facioni ; Moore, Stephen Stewart</creatorcontrib><description>Growth factors such as bone morphogenetic proteins 6, 7, 15, and two isoforms of transforming growth factor-beta (BMP6, BMP7, BMP15, TGFB1, and TGFB2), and insulin-like growth factor system act as local regulators of ovarian follicular development. To elucidate if these factors as well as others candidate genes, such as estrogen receptor 1 (
), growth differentiation factor 9 (
), follicle-stimulating hormone receptor (
), luteinizing hormone receptor (
), bone morphogenetic protein receptor, type 2 (
), type 1 insulin-like growth factor receptor (
), and key steroidogenic enzymes cytochrome P450 aromatase and 3-β-hydroxysteroid dehydrogenase (
and
) could modulate or influence diestrus on the onset of puberty in Brahman heifers, their ovarian mRNA expression was measured before and after puberty (luteal phase). Six postpubertal (POST) heifers were euthanized on the luteal phase of their second cycle, confirmed by corpus luteum observation, and six prepubertal (PRE) heifers were euthanized in the same day. Quantitative real-time PCR analysis showed that the expression of
, and
mRNA was greater in PRE heifers, when contrasted to POST heifers. The expression of
and
was lower in PRE heifers. Differential expression of ovarian genes could be associated with changes in follicular dynamics and different cell populations that have emerged as consequence of puberty and the luteal phase. The emerging hypothesis is that
and
are co-expressed and may modulate the expression of
and
, and
.
could influence the downregulation of
and upregulation of
and
, which mediates the follicular dynamics in heifer ovaries. Upregulation of
expression prepuberty, compared to postpuberty diestrus, correlates with increased levels
and
. Thus,
and
may play synergic roles and were predicted to interact, from the expression data (
= 0.07,
= 0.84). The role of these co-expressed genes in puberty and heifers luteal phase merits further research.</description><identifier>ISSN: 2297-1769</identifier><identifier>EISSN: 2297-1769</identifier><identifier>DOI: 10.3389/fvets.2016.00094</identifier><identifier>PMID: 27803898</identifier><language>eng</language><publisher>Switzerland: Frontiers Media S.A</publisher><subject>Veterinary Science</subject><ispartof>Frontiers in veterinary science, 2016-10, Vol.3, p.94-94</ispartof><rights>Copyright © 2016 Weller, Fortes, Porto-Neto, Kelly, Venus, Kidd, do Rego, Edwards, Boe-Hansen, Piper, Lehnert, Guimarães and Moore. 2016 Weller, Fortes, Porto-Neto, Kelly, Venus, Kidd, do Rego, Edwards, Boe-Hansen, Piper, Lehnert, Guimarães and Moore</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-1704a7922d2d093032919274c4930d0f14973858afe5d3ed3090055f3aec72e93</citedby><cites>FETCH-LOGICAL-c396t-1704a7922d2d093032919274c4930d0f14973858afe5d3ed3090055f3aec72e93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5067528/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5067528/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27922,27923,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27803898$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Weller, Mayara Morena Del Cambre Amaral</creatorcontrib><creatorcontrib>Fortes, Marina Rufino S</creatorcontrib><creatorcontrib>Porto-Neto, Laercio R</creatorcontrib><creatorcontrib>Kelly, Matthew</creatorcontrib><creatorcontrib>Venus, Bronwyn</creatorcontrib><creatorcontrib>Kidd, Lisa</creatorcontrib><creatorcontrib>do Rego, João Paulo Arcelino</creatorcontrib><creatorcontrib>Edwards, Sophia</creatorcontrib><creatorcontrib>Boe-Hansen, Gry B</creatorcontrib><creatorcontrib>Piper, Emily</creatorcontrib><creatorcontrib>Lehnert, Sigrid A</creatorcontrib><creatorcontrib>Guimarães, Simone Eliza Facioni</creatorcontrib><creatorcontrib>Moore, Stephen Stewart</creatorcontrib><title>Candidate Gene Expression in Bos indicus Ovarian Tissues: Prepubertal and Postpubertal Heifers in Diestrus</title><title>Frontiers in veterinary science</title><addtitle>Front Vet Sci</addtitle><description>Growth factors such as bone morphogenetic proteins 6, 7, 15, and two isoforms of transforming growth factor-beta (BMP6, BMP7, BMP15, TGFB1, and TGFB2), and insulin-like growth factor system act as local regulators of ovarian follicular development. To elucidate if these factors as well as others candidate genes, such as estrogen receptor 1 (
), growth differentiation factor 9 (
), follicle-stimulating hormone receptor (
), luteinizing hormone receptor (
), bone morphogenetic protein receptor, type 2 (
), type 1 insulin-like growth factor receptor (
), and key steroidogenic enzymes cytochrome P450 aromatase and 3-β-hydroxysteroid dehydrogenase (
and
) could modulate or influence diestrus on the onset of puberty in Brahman heifers, their ovarian mRNA expression was measured before and after puberty (luteal phase). Six postpubertal (POST) heifers were euthanized on the luteal phase of their second cycle, confirmed by corpus luteum observation, and six prepubertal (PRE) heifers were euthanized in the same day. Quantitative real-time PCR analysis showed that the expression of
, and
mRNA was greater in PRE heifers, when contrasted to POST heifers. The expression of
and
was lower in PRE heifers. Differential expression of ovarian genes could be associated with changes in follicular dynamics and different cell populations that have emerged as consequence of puberty and the luteal phase. The emerging hypothesis is that
and
are co-expressed and may modulate the expression of
and
, and
.
could influence the downregulation of
and upregulation of
and
, which mediates the follicular dynamics in heifer ovaries. Upregulation of
expression prepuberty, compared to postpuberty diestrus, correlates with increased levels
and
. Thus,
and
may play synergic roles and were predicted to interact, from the expression data (
= 0.07,
= 0.84). The role of these co-expressed genes in puberty and heifers luteal phase merits further research.</description><subject>Veterinary Science</subject><issn>2297-1769</issn><issn>2297-1769</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNpVUT1PwzAQtRCIosLOhDyytFzspI4ZkKCUFqlSGWC2THwBozQpvqSCf4_LR1Wmu7Pfe_dOj7HTBIZS5vqiXGNLQwHJaAgAOt1jR0JoNUjUSO_v9D12QvQWIUmWKpnDIesJlUOUyI_Y29jWzjvbIp9ijXzysQpI5Jua-5rfNBSL80VHfLG2wduaP3qiDumSPwRcdc8YWlvxKMIfGmq3DzP0JYYNm996pDZ0dMwOSlsRnvzWPnu6mzyOZ4P5Yno_vp4PCqlHbbQMqVVaCCccaAlS6EQLlRZpHByUSarjFVluS8ycRCdBA2RZKS0WSqCWfXb1oxvNLNEVWLfBVmYV_NKGT9NYb_7_1P7VvDRrk8FIZSKPAue_AqF5j6e2ZumpwKqyNTYdmSSXWcQptYHCD7QIDVHAcrsmAbNJyXynZDYpme-UIuVs196W8JeJ_AL3TY_e</recordid><startdate>20161018</startdate><enddate>20161018</enddate><creator>Weller, Mayara Morena Del Cambre Amaral</creator><creator>Fortes, Marina Rufino S</creator><creator>Porto-Neto, Laercio R</creator><creator>Kelly, Matthew</creator><creator>Venus, Bronwyn</creator><creator>Kidd, Lisa</creator><creator>do Rego, João Paulo Arcelino</creator><creator>Edwards, Sophia</creator><creator>Boe-Hansen, Gry B</creator><creator>Piper, Emily</creator><creator>Lehnert, Sigrid A</creator><creator>Guimarães, Simone Eliza Facioni</creator><creator>Moore, Stephen Stewart</creator><general>Frontiers Media S.A</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20161018</creationdate><title>Candidate Gene Expression in Bos indicus Ovarian Tissues: Prepubertal and Postpubertal Heifers in Diestrus</title><author>Weller, Mayara Morena Del Cambre Amaral ; Fortes, Marina Rufino S ; Porto-Neto, Laercio R ; Kelly, Matthew ; Venus, Bronwyn ; Kidd, Lisa ; do Rego, João Paulo Arcelino ; Edwards, Sophia ; Boe-Hansen, Gry B ; Piper, Emily ; Lehnert, Sigrid A ; Guimarães, Simone Eliza Facioni ; Moore, Stephen Stewart</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-1704a7922d2d093032919274c4930d0f14973858afe5d3ed3090055f3aec72e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Veterinary Science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Weller, Mayara Morena Del Cambre Amaral</creatorcontrib><creatorcontrib>Fortes, Marina Rufino S</creatorcontrib><creatorcontrib>Porto-Neto, Laercio R</creatorcontrib><creatorcontrib>Kelly, Matthew</creatorcontrib><creatorcontrib>Venus, Bronwyn</creatorcontrib><creatorcontrib>Kidd, Lisa</creatorcontrib><creatorcontrib>do Rego, João Paulo Arcelino</creatorcontrib><creatorcontrib>Edwards, Sophia</creatorcontrib><creatorcontrib>Boe-Hansen, Gry B</creatorcontrib><creatorcontrib>Piper, Emily</creatorcontrib><creatorcontrib>Lehnert, Sigrid A</creatorcontrib><creatorcontrib>Guimarães, Simone Eliza Facioni</creatorcontrib><creatorcontrib>Moore, Stephen Stewart</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Frontiers in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Weller, Mayara Morena Del Cambre Amaral</au><au>Fortes, Marina Rufino S</au><au>Porto-Neto, Laercio R</au><au>Kelly, Matthew</au><au>Venus, Bronwyn</au><au>Kidd, Lisa</au><au>do Rego, João Paulo Arcelino</au><au>Edwards, Sophia</au><au>Boe-Hansen, Gry B</au><au>Piper, Emily</au><au>Lehnert, Sigrid A</au><au>Guimarães, Simone Eliza Facioni</au><au>Moore, Stephen Stewart</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Candidate Gene Expression in Bos indicus Ovarian Tissues: Prepubertal and Postpubertal Heifers in Diestrus</atitle><jtitle>Frontiers in veterinary science</jtitle><addtitle>Front Vet Sci</addtitle><date>2016-10-18</date><risdate>2016</risdate><volume>3</volume><spage>94</spage><epage>94</epage><pages>94-94</pages><issn>2297-1769</issn><eissn>2297-1769</eissn><abstract>Growth factors such as bone morphogenetic proteins 6, 7, 15, and two isoforms of transforming growth factor-beta (BMP6, BMP7, BMP15, TGFB1, and TGFB2), and insulin-like growth factor system act as local regulators of ovarian follicular development. To elucidate if these factors as well as others candidate genes, such as estrogen receptor 1 (
), growth differentiation factor 9 (
), follicle-stimulating hormone receptor (
), luteinizing hormone receptor (
), bone morphogenetic protein receptor, type 2 (
), type 1 insulin-like growth factor receptor (
), and key steroidogenic enzymes cytochrome P450 aromatase and 3-β-hydroxysteroid dehydrogenase (
and
) could modulate or influence diestrus on the onset of puberty in Brahman heifers, their ovarian mRNA expression was measured before and after puberty (luteal phase). Six postpubertal (POST) heifers were euthanized on the luteal phase of their second cycle, confirmed by corpus luteum observation, and six prepubertal (PRE) heifers were euthanized in the same day. Quantitative real-time PCR analysis showed that the expression of
, and
mRNA was greater in PRE heifers, when contrasted to POST heifers. The expression of
and
was lower in PRE heifers. Differential expression of ovarian genes could be associated with changes in follicular dynamics and different cell populations that have emerged as consequence of puberty and the luteal phase. The emerging hypothesis is that
and
are co-expressed and may modulate the expression of
and
, and
.
could influence the downregulation of
and upregulation of
and
, which mediates the follicular dynamics in heifer ovaries. Upregulation of
expression prepuberty, compared to postpuberty diestrus, correlates with increased levels
and
. Thus,
and
may play synergic roles and were predicted to interact, from the expression data (
= 0.07,
= 0.84). The role of these co-expressed genes in puberty and heifers luteal phase merits further research.</abstract><cop>Switzerland</cop><pub>Frontiers Media S.A</pub><pmid>27803898</pmid><doi>10.3389/fvets.2016.00094</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5067528 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Veterinary Science |
| title | Candidate Gene Expression in Bos indicus Ovarian Tissues: Prepubertal and Postpubertal Heifers in Diestrus |
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