High-throughput measurement of single-cell growth rates using serial microfluidic mass sensor arrays

Heterogeneity in growth phenotypes and drug susceptibility in bacterial and mammalian cells are assayed at the single-cell level using multiplexed resonant mass sensors. Methods to rapidly assess cell growth would be useful for many applications, including drug susceptibility testing, but current te...

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Veröffentlicht in:	Nature biotechnology 2016-10, Vol.34 (10), p.1052-1059
Hauptverfasser:	Cermak, Nathan, Olcum, Selim, Delgado, Francisco Feijó, Wasserman, Steven C, Payer, Kristofor R, A Murakami, Mark, Knudsen, Scott M, Kimmerling, Robert J, Stevens, Mark M, Kikuchi, Yuki, Sandikci, Arzu, Ogawa, Masaaki, Agache, Vincent, Baléras, François, Weinstock, David M, Manalis, Scott R
Format:	Artikel
Sprache:	eng
Schlagworte:	13/31 631/57 631/80 639/166/985 Agriculture Antibiotics Bioinformatics Biomedical engineering Biomedical Engineering/Biotechnology Biomedicine Biophysics Biotechnology Cell growth Cell Proliferation - drug effects Cell Proliferation - physiology Drug Evaluation, Preclinical - instrumentation Drug Evaluation, Preclinical - methods E coli Enterococcus faecalis Equipment Design Equipment Failure Analysis Escherichia coli Fluid mechanics Growth Growth kinetics High-Throughput Screening Assays - instrumentation High-Throughput Screening Assays - methods Lab-On-A-Chip Devices Leukemia Life Sciences Mammals Measurement Micro-Electrical-Mechanical Systems - instrumentation Micro-Electrical-Mechanical Systems - methods Peptides Physiological aspects Reproducibility of Results Sensitivity and Specificity Sensors Subpopulations T cells Transducers Yeasts
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