Evaluation of Malaria Diagnoses Using a Handheld Light Microscope in a Community-Based Setting in Rural Côte d'Ivoire
Portable microscopy may facilitate quality diagnostic care in resource-constrained settings. We compared a handheld light microscope (Newton Nm1) with a mobile phone attachment to conventional light microscopy for the detection of Plasmodium falciparum in a cross-sectional study in rural Côte d'...
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| Veröffentlicht in: | The American journal of tropical medicine and hygiene 2016-10, Vol.95 (4), p.831-834 |
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| container_title | The American journal of tropical medicine and hygiene |
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| creator | Coulibaly, Jean T Ouattara, Mamadou Keiser, Jennifer Bonfoh, Bassirou N'Goran, Eliézer K Andrews, Jason R Bogoch, Isaac I |
| description | Portable microscopy may facilitate quality diagnostic care in resource-constrained settings. We compared a handheld light microscope (Newton Nm1) with a mobile phone attachment to conventional light microscopy for the detection of Plasmodium falciparum in a cross-sectional study in rural Côte d'Ivoire. Single Giemsa-stained thick blood film from 223 individuals were prepared and read by local laboratory technicians on both microscopes under 1,000× magnification with oil. Of the 223 samples, 162 (72.6%) were P. falciparum positive, and the overall mean parasite count was 1,392/μL of blood. Sensitivity and specificity of the handheld microscope was 80.2% (95% confidence interval [CI]: 73.1-85.9%) and 100.0% (95% CI: 92.6-100.0%), respectively, with a positive and negative predictive value of 100.0% (95% CI: 96.4-100.0%) and 65.6% (95% CI: 54.9-74.9%), respectively. If sensitivity can be improved, handheld light microscopy may become a valuable public health tool for P. falciparum diagnosis. |
| doi_str_mv | 10.4269/ajtmh.16-0328 |
| format | Article |
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We compared a handheld light microscope (Newton Nm1) with a mobile phone attachment to conventional light microscopy for the detection of Plasmodium falciparum in a cross-sectional study in rural Côte d'Ivoire. Single Giemsa-stained thick blood film from 223 individuals were prepared and read by local laboratory technicians on both microscopes under 1,000× magnification with oil. Of the 223 samples, 162 (72.6%) were P. falciparum positive, and the overall mean parasite count was 1,392/μL of blood. Sensitivity and specificity of the handheld microscope was 80.2% (95% confidence interval [CI]: 73.1-85.9%) and 100.0% (95% CI: 92.6-100.0%), respectively, with a positive and negative predictive value of 100.0% (95% CI: 96.4-100.0%) and 65.6% (95% CI: 54.9-74.9%), respectively. 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We compared a handheld light microscope (Newton Nm1) with a mobile phone attachment to conventional light microscopy for the detection of Plasmodium falciparum in a cross-sectional study in rural Côte d'Ivoire. Single Giemsa-stained thick blood film from 223 individuals were prepared and read by local laboratory technicians on both microscopes under 1,000× magnification with oil. Of the 223 samples, 162 (72.6%) were P. falciparum positive, and the overall mean parasite count was 1,392/μL of blood. Sensitivity and specificity of the handheld microscope was 80.2% (95% confidence interval [CI]: 73.1-85.9%) and 100.0% (95% CI: 92.6-100.0%), respectively, with a positive and negative predictive value of 100.0% (95% CI: 96.4-100.0%) and 65.6% (95% CI: 54.9-74.9%), respectively. If sensitivity can be improved, handheld light microscopy may become a valuable public health tool for P. falciparum diagnosis.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Cell Phone</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cote d'Ivoire - epidemiology</subject><subject>Cross-Sectional Studies</subject><subject>Female</subject><subject>Humans</subject><subject>Malaria, Falciparum - diagnosis</subject><subject>Malaria, Falciparum - epidemiology</subject><subject>Malaria, Falciparum - parasitology</subject><subject>Male</subject><subject>Microscopy - instrumentation</subject><subject>Microscopy - methods</subject><subject>Parasitemia</subject><subject>Plasmodium falciparum</subject><subject>Plasmodium falciparum - isolation & purification</subject><subject>Rural Population</subject><subject>Sensitivity and Specificity</subject><subject>Young Adult</subject><issn>0002-9637</issn><issn>1476-1645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EotOWJVvkHWxSru3EdjZIdChtpamQKF1bN44z4yqxh9gZqe_FG_BizdAfwY7Vle75fHSuDyFvGZyUXNYf8TYPmxMmCxBcvyALVipZMFlWL8kCAHhRS6EOyGFKtwBMc8ZekwOuKq7m_YLsznbYT5h9DDR29Ap7HD3SLx7XISaX6E3yYU2RXmBoN65v6cqvN5leeTvGZOPWUR9meRmHYQo-3xWnmFxLr13O-4ez-H0asafL37-yo-37y130ozsmrzrsk3vzOI_IzdezH8uLYvXt_HL5eVXYkkMueCNFayvboeTQ1A3WVs65G2d1UyrosJatBmwkb4WyrOas6SomkAPIWjMnjsinB9_t1AyutS7kOYzZjn7A8c5E9OZfJfiNWcedqUBypfls8OHRYIw_J5eyGXyyru8xuDglw3QFSgHX7D9QUYmqlFrMaPGA7j8xja57TsTA7Gs1f2o1TJp9rTP_7u8znumnHsU94iKgnA</recordid><startdate>20161005</startdate><enddate>20161005</enddate><creator>Coulibaly, Jean T</creator><creator>Ouattara, Mamadou</creator><creator>Keiser, Jennifer</creator><creator>Bonfoh, Bassirou</creator><creator>N'Goran, Eliézer K</creator><creator>Andrews, Jason R</creator><creator>Bogoch, Isaac I</creator><general>The American Society of Tropical Medicine and Hygiene</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T2</scope><scope>7U2</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope><scope>5PM</scope></search><sort><creationdate>20161005</creationdate><title>Evaluation of Malaria Diagnoses Using a Handheld Light Microscope in a Community-Based Setting in Rural Côte d'Ivoire</title><author>Coulibaly, Jean T ; 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We compared a handheld light microscope (Newton Nm1) with a mobile phone attachment to conventional light microscopy for the detection of Plasmodium falciparum in a cross-sectional study in rural Côte d'Ivoire. Single Giemsa-stained thick blood film from 223 individuals were prepared and read by local laboratory technicians on both microscopes under 1,000× magnification with oil. Of the 223 samples, 162 (72.6%) were P. falciparum positive, and the overall mean parasite count was 1,392/μL of blood. Sensitivity and specificity of the handheld microscope was 80.2% (95% confidence interval [CI]: 73.1-85.9%) and 100.0% (95% CI: 92.6-100.0%), respectively, with a positive and negative predictive value of 100.0% (95% CI: 96.4-100.0%) and 65.6% (95% CI: 54.9-74.9%), respectively. 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| subjects | Adolescent Adult Cell Phone Child Child, Preschool Cote d'Ivoire - epidemiology Cross-Sectional Studies Female Humans Malaria, Falciparum - diagnosis Malaria, Falciparum - epidemiology Malaria, Falciparum - parasitology Male Microscopy - instrumentation Microscopy - methods Parasitemia Plasmodium falciparum Plasmodium falciparum - isolation & purification Rural Population Sensitivity and Specificity Young Adult |
| title | Evaluation of Malaria Diagnoses Using a Handheld Light Microscope in a Community-Based Setting in Rural Côte d'Ivoire |
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