Genome-scale analysis of the cotton KCS gene family revealed a binary mode of action for gibberellin A regulated fiber growth
Production of b-ketoacyl-Co A, which is catalyzed by 3-ketoacyl-CoA synthase（KCS）, is the first step in very long chain fatty acid（VLCFA） biosynthesis. Here we identified 58 KCS genes from Gossypium hirsutum, 31 from G. arboreum and 33 from G. raimondii by searching the assembled cotton genomes. The...
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| Veröffentlicht in: | Journal of integrative plant biology 2016-06, Vol.58 (6), p.577-589 |
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| creator | Xiao, Guang-Hui Wang, Kun Huang, Gai Zhu, Yu-Xian |
| description | Production of b-ketoacyl-Co A, which is catalyzed by 3-ketoacyl-CoA synthase（KCS）, is the first step in very long chain fatty acid（VLCFA） biosynthesis. Here we identified 58 KCS genes from Gossypium hirsutum, 31 from G. arboreum and 33 from G. raimondii by searching the assembled cotton genomes. The gene family was divided into the plant-specific FAE1-type and the more general ELO-type. KCS transcripts were widely expressed and 32 of them showed distinct subgenome-specific expressions in one or more cotton tissues/organs studied. Six Gh KCS genes rescued the lethality of elo2Δelo3Δ yeast double mutant,indicating that this gene family possesses diversified functions.Most KCS genes with GA-responsive elements（GAREs） in the promoters were significantly upregulated by gibberellin A_3（GA）.Exogenous GA_3 not only promoted fiber length, but also increased the thickness of cell walls significantly. GAREs present also in the promoters of several cellulose synthase（CesA） genes required for cell wall biosynthesis and they were all induced significantly by GA_3. Because GA treatment resulted in longer cotton fibers with thicker cell walls and higher dry weight per unit cell length, we suggest that it may regulate fiber elongation upstream of the VLCFA-ethylene pathway and also in the downstream steps towards cell wall synthesis. |
| doi_str_mv | 10.1111/jipb.12429 |
| format | Article |
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Here we identified 58 KCS genes from Gossypium hirsutum, 31 from G. arboreum and 33 from G. raimondii by searching the assembled cotton genomes. The gene family was divided into the plant-specific FAE1-type and the more general ELO-type. KCS transcripts were widely expressed and 32 of them showed distinct subgenome-specific expressions in one or more cotton tissues/organs studied. Six Gh KCS genes rescued the lethality of elo2Δelo3Δ yeast double mutant,indicating that this gene family possesses diversified functions.Most KCS genes with GA-responsive elements（GAREs） in the promoters were significantly upregulated by gibberellin A_3（GA）.Exogenous GA_3 not only promoted fiber length, but also increased the thickness of cell walls significantly. GAREs present also in the promoters of several cellulose synthase（CesA） genes required for cell wall biosynthesis and they were all induced significantly by GA_3. Because GA treatment resulted in longer cotton fibers with thicker cell walls and higher dry weight per unit cell length, we suggest that it may regulate fiber elongation upstream of the VLCFA-ethylene pathway and also in the downstream steps towards cell wall synthesis.</description><identifier>ISSN: 1672-9072</identifier><identifier>EISSN: 1744-7909</identifier><identifier>DOI: 10.1111/jipb.12429</identifier><identifier>PMID: 26399709</identifier><language>eng</language><publisher>China (Republic : 1949- ): Blackwell Publishing Ltd</publisher><subject>3-Ketoacyl-CoA synthase ; cotton ; expression patterns ; Fatty Acids - metabolism ; Gene Expression Profiling ; Gene Expression Regulation, Plant - drug effects ; Gene Expression Regulation, Plant - genetics ; gibberellins ; Gibberellins - pharmacology ; Glucosyltransferases - genetics ; Glucosyltransferases - metabolism ; Gossypium - drug effects ; Gossypium - growth & development ; Gossypium - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; very long chain fatty acids ; 二进制模式 ; 基因家族 ; 基因组 ; 棉花 ; 生物合成 ; 生长作用 ; 纤维长度 ; 赤霉素</subject><ispartof>Journal of integrative plant biology, 2016-06, Vol.58 (6), p.577-589</ispartof><rights>2015 The Authors. published by Wiley Publishing Asia Pty Ltd on behalf of Institute of Botany, Chinese Academy of Sciences.</rights><rights>2015 The Authors. Journal of Integrative Plant Biology published by Wiley Publishing Asia Pty Ltd on behalf of Institute of Botany, Chinese Academy of Sciences.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5159-697fc319d812f3e5a90a167dcf6dba5154daf81502ba00a584aa10659ee9402e3</citedby><cites>FETCH-LOGICAL-c5159-697fc319d812f3e5a90a167dcf6dba5154daf81502ba00a584aa10659ee9402e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/94176A/94176A.jpg</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjipb.12429$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjipb.12429$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26399709$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xiao, Guang-Hui</creatorcontrib><creatorcontrib>Wang, Kun</creatorcontrib><creatorcontrib>Huang, Gai</creatorcontrib><creatorcontrib>Zhu, Yu-Xian</creatorcontrib><title>Genome-scale analysis of the cotton KCS gene family revealed a binary mode of action for gibberellin A regulated fiber growth</title><title>Journal of integrative plant biology</title><addtitle>Journal of Integrative Plant Biology</addtitle><description>Production of b-ketoacyl-Co A, which is catalyzed by 3-ketoacyl-CoA synthase（KCS）, is the first step in very long chain fatty acid（VLCFA） biosynthesis. Here we identified 58 KCS genes from Gossypium hirsutum, 31 from G. arboreum and 33 from G. raimondii by searching the assembled cotton genomes. The gene family was divided into the plant-specific FAE1-type and the more general ELO-type. KCS transcripts were widely expressed and 32 of them showed distinct subgenome-specific expressions in one or more cotton tissues/organs studied. Six Gh KCS genes rescued the lethality of elo2Δelo3Δ yeast double mutant,indicating that this gene family possesses diversified functions.Most KCS genes with GA-responsive elements（GAREs） in the promoters were significantly upregulated by gibberellin A_3（GA）.Exogenous GA_3 not only promoted fiber length, but also increased the thickness of cell walls significantly. GAREs present also in the promoters of several cellulose synthase（CesA） genes required for cell wall biosynthesis and they were all induced significantly by GA_3. Because GA treatment resulted in longer cotton fibers with thicker cell walls and higher dry weight per unit cell length, we suggest that it may regulate fiber elongation upstream of the VLCFA-ethylene pathway and also in the downstream steps towards cell wall synthesis.</description><subject>3-Ketoacyl-CoA synthase</subject><subject>cotton</subject><subject>expression patterns</subject><subject>Fatty Acids - metabolism</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Gene Expression Regulation, Plant - genetics</subject><subject>gibberellins</subject><subject>Gibberellins - pharmacology</subject><subject>Glucosyltransferases - genetics</subject><subject>Glucosyltransferases - metabolism</subject><subject>Gossypium - drug effects</subject><subject>Gossypium - growth & development</subject><subject>Gossypium - metabolism</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>very long chain fatty acids</subject><subject>二进制模式</subject><subject>基因家族</subject><subject>基因组</subject><subject>棉花</subject><subject>生物合成</subject><subject>生长作用</subject><subject>纤维长度</subject><subject>赤霉素</subject><issn>1672-9072</issn><issn>1744-7909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNp9kUuP0zAUhSMEYoaBDT8AWUhskDLYTuzUG6ShgjIw4iEGsbRukuvUJbGLnU6nSPx3XPoQbPDGlv2dc8_1zbLHjJ6ztF4s7LI-Z7zk6k52yqqyzCtF1d10lhXPFa34SfYgxgWlxYRKfj874bJQqqLqNPs1Q-cHzGMDPRJw0G-ijcQbMs6RNH4cvSPvp19Ihw6JgcH2GxLwBhPeEiC1dRA2ZPAtbkXQjDYJjA-ks3WNAfveOnKRJN2qhzFpjE3XpAt-Pc4fZvcM9BEf7fez7Oub19fTt_nVx9nl9OIqbwQTKpeqMk3BVDth3BQoQFFIvbWNkW0NCSlbMBMmKK-BUhCTEoBRKRSiKinH4ix7ufNdruoB2wbdGKDXy2CHlF57sPrfF2fnuvM3WlDJGC2TwbOdwRqcAdfphV-F9FlR_1zf1pwySSWlMnHPd1wTfIwBzbEGo3o7LL0dlv4zrAQ_-TvVET1MJwFsX9X2uPmPlX53-enVwTTfaWwc8faogfBdy6qohP72YaZl-ZlVSlzrbeKn-8Rz77ofNvV20EipClGJCSt-A_oLu2Q</recordid><startdate>201606</startdate><enddate>201606</enddate><creator>Xiao, Guang-Hui</creator><creator>Wang, Kun</creator><creator>Huang, Gai</creator><creator>Zhu, Yu-Xian</creator><general>Blackwell Publishing Ltd</general><general>The State Key Laboratory of Protein and Plant Gene Research, Col ege of Life Sciences, Peking University, Beijing 100871, China%Institute for Advanced Studies/Col ege of Life Sciences, Wuhan University, Wuhan 430072, China</general><general>John Wiley and Sons Inc</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>BSCLL</scope><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>201606</creationdate><title>Genome-scale analysis of the cotton KCS gene family revealed a binary mode of action for gibberellin A regulated fiber growth</title><author>Xiao, Guang-Hui ; Wang, Kun ; Huang, Gai ; Zhu, Yu-Xian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5159-697fc319d812f3e5a90a167dcf6dba5154daf81502ba00a584aa10659ee9402e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>3-Ketoacyl-CoA synthase</topic><topic>cotton</topic><topic>expression patterns</topic><topic>Fatty Acids - metabolism</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Gene Expression Regulation, Plant - genetics</topic><topic>gibberellins</topic><topic>Gibberellins - pharmacology</topic><topic>Glucosyltransferases - genetics</topic><topic>Glucosyltransferases - metabolism</topic><topic>Gossypium - drug effects</topic><topic>Gossypium - growth & development</topic><topic>Gossypium - metabolism</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>very long chain fatty acids</topic><topic>二进制模式</topic><topic>基因家族</topic><topic>基因组</topic><topic>棉花</topic><topic>生物合成</topic><topic>生长作用</topic><topic>纤维长度</topic><topic>赤霉素</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xiao, Guang-Hui</creatorcontrib><creatorcontrib>Wang, Kun</creatorcontrib><creatorcontrib>Huang, Gai</creatorcontrib><creatorcontrib>Zhu, Yu-Xian</creatorcontrib><collection>维普_期刊</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>维普中文期刊数据库</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Istex</collection><collection>Wiley Open Access Journals</collection><collection>Wiley Free Archive</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of integrative plant biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xiao, Guang-Hui</au><au>Wang, Kun</au><au>Huang, Gai</au><au>Zhu, Yu-Xian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome-scale analysis of the cotton KCS gene family revealed a binary mode of action for gibberellin A regulated fiber growth</atitle><jtitle>Journal of integrative plant biology</jtitle><addtitle>Journal of Integrative Plant Biology</addtitle><date>2016-06</date><risdate>2016</risdate><volume>58</volume><issue>6</issue><spage>577</spage><epage>589</epage><pages>577-589</pages><issn>1672-9072</issn><eissn>1744-7909</eissn><abstract>Production of b-ketoacyl-Co A, which is catalyzed by 3-ketoacyl-CoA synthase（KCS）, is the first step in very long chain fatty acid（VLCFA） biosynthesis. Here we identified 58 KCS genes from Gossypium hirsutum, 31 from G. arboreum and 33 from G. raimondii by searching the assembled cotton genomes. The gene family was divided into the plant-specific FAE1-type and the more general ELO-type. KCS transcripts were widely expressed and 32 of them showed distinct subgenome-specific expressions in one or more cotton tissues/organs studied. Six Gh KCS genes rescued the lethality of elo2Δelo3Δ yeast double mutant,indicating that this gene family possesses diversified functions.Most KCS genes with GA-responsive elements（GAREs） in the promoters were significantly upregulated by gibberellin A_3（GA）.Exogenous GA_3 not only promoted fiber length, but also increased the thickness of cell walls significantly. GAREs present also in the promoters of several cellulose synthase（CesA） genes required for cell wall biosynthesis and they were all induced significantly by GA_3. Because GA treatment resulted in longer cotton fibers with thicker cell walls and higher dry weight per unit cell length, we suggest that it may regulate fiber elongation upstream of the VLCFA-ethylene pathway and also in the downstream steps towards cell wall synthesis.</abstract><cop>China (Republic : 1949- )</cop><pub>Blackwell Publishing Ltd</pub><pmid>26399709</pmid><doi>10.1111/jipb.12429</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of integrative plant biology, 2016-06, Vol.58 (6), p.577-589 |
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| source | Wiley-Blackwell Journals; MEDLINE; Alma/SFX Local Collection |
| subjects | 3-Ketoacyl-CoA synthase cotton expression patterns Fatty Acids - metabolism Gene Expression Profiling Gene Expression Regulation, Plant - drug effects Gene Expression Regulation, Plant - genetics gibberellins Gibberellins - pharmacology Glucosyltransferases - genetics Glucosyltransferases - metabolism Gossypium - drug effects Gossypium - growth & development Gossypium - metabolism Plant Proteins - genetics Plant Proteins - metabolism very long chain fatty acids 二进制模式 基因家族 基因组 棉花 生物合成 生长作用 纤维长度 赤霉素 |
| title | Genome-scale analysis of the cotton KCS gene family revealed a binary mode of action for gibberellin A regulated fiber growth |
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