HIV-1 Vpr N-terminal tagging affects alternative splicing of the viral genome

To facilitate studies on Vpr function in replicating HIV-1, we aimed to tag the protein in an infectious virus. First we showed that N-, but not C-terminal HA/FLAG tagging of Vpr protein preserves Vpr cytopathicity. Cloning the tags into proviral DNA however ablated viral production and replication....

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Veröffentlicht in:	Scientific reports 2016-10, Vol.6 (1), p.34573-34573, Article 34573
Hauptverfasser:	Baeyens, Ann, Naessens, Evelien, Van Nuffel, Anouk, Weening, Karin E., Reilly, Anne-Marie, Claeys, Eva, Trypsteen, Wim, Vandekerckhove, Linos, Eyckerman, Sven, Gevaert, Kris, Verhasselt, Bruno
Format:	Artikel
Sprache:	eng
Schlagworte:	631/326/596/1787 631/326/596/2148 Alternative splicing Alternative Splicing - physiology Cell Line Cloning Deoxyribonucleic acid DNA Genome, Viral - physiology Genomes HIV-1 - genetics HIV-1 - metabolism Humanities and Social Sciences Humans Infectivity mRNA multidisciplinary Nucleotide sequence Proteins Regulatory sequences RNA Folding - physiology RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Viral - genetics RNA, Viral - metabolism Science Science (multidisciplinary) vpr Gene Products, Human Immunodeficiency Virus - genetics vpr Gene Products, Human Immunodeficiency Virus - metabolism Vpr protein
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