Changes in the Dermal Structure during Cultured Epidermal Autograft Engraftment Process

Changes in the Dermal Structure during Cultured Epidermal Autograft Engraftment Process

BACKGROUND:The use of cultured epithelial autografts for the treatment of extensive burn wounds has become popular in recent years. We examined extensive burn wounds in 14 patients by using a combination of autograft and cultured epithelial autografts developed in Japan (JACE). METHODS:We undertook...

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Veröffentlicht in:Plastic and reconstructive surgery. Global open 2016-09, Vol.4 (9), p.e870-e870
Hauptverfasser: Hayashi, Minoru, Muramatsu, Hideyuki, Nakano, Minoru, Yamamoto, Naoto, Tokunaka, Ryohei, Umezawa, Kazuya, Hamajima, Akito, Araki, Natsue, Yoshimoto, Shinya
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container_end_page e870
container_issue 9
container_start_page e870
container_title Plastic and reconstructive surgery. Global open
container_volume 4
creator Hayashi, Minoru
Muramatsu, Hideyuki
Nakano, Minoru
Yamamoto, Naoto
Tokunaka, Ryohei
Umezawa, Kazuya
Hamajima, Akito
Araki, Natsue
Yoshimoto, Shinya
description BACKGROUND:The use of cultured epithelial autografts for the treatment of extensive burn wounds has become popular in recent years. We examined extensive burn wounds in 14 patients by using a combination of autograft and cultured epithelial autografts developed in Japan (JACE). METHODS:We undertook a skin biopsy at 2, 4, and 6 weeks after transplantation with JACE. By using electron microscopy we observed the engraftment process. RESULTS:In transmission electron microscope findings, we recognized the engraftment process of JACE. Keratinocytes matured gradually. Collagen fibers formed thick bundles in the dermis layer. In scanning electron microscope findings, we observed papillary dermis development on the artificial dermis. CONCLUSIONS:After managing wound bed preparation by using artificial dermis, we were able to recognize the good result of grafting JACE on meshed 6:1 split thickness autografts. This is because the auto dermis from autograft extended under the JACE, binding between JACE, and the dermis became strong.
doi_str_mv 10.1097/GOX.0000000000000838
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We examined extensive burn wounds in 14 patients by using a combination of autograft and cultured epithelial autografts developed in Japan (JACE). METHODS:We undertook a skin biopsy at 2, 4, and 6 weeks after transplantation with JACE. By using electron microscopy we observed the engraftment process. RESULTS:In transmission electron microscope findings, we recognized the engraftment process of JACE. Keratinocytes matured gradually. Collagen fibers formed thick bundles in the dermis layer. In scanning electron microscope findings, we observed papillary dermis development on the artificial dermis. CONCLUSIONS:After managing wound bed preparation by using artificial dermis, we were able to recognize the good result of grafting JACE on meshed 6:1 split thickness autografts. 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title Changes in the Dermal Structure during Cultured Epidermal Autograft Engraftment Process
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