Diaphanous gene mutation affects spiral cleavage and chirality in snails
L-R (left and right) symmetry breaking during embryogenesis and the establishment of asymmetric body plan are key issues in developmental biology, but the onset including the handedness-determining gene locus still remains unknown. Using pure dextral (DD) and sinistral (dd) strains of the pond snail...
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| Veröffentlicht in: | Scientific reports 2016-10, Vol.6 (1), p.34809-34809, Article 34809 |
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| creator | Kuroda, Reiko Fujikura, Kohei Abe, Masanori Hosoiri, Yuji Asakawa, Shuichi Shimizu, Miho Umeda, Shin Ichikawa, Futaba Takahashi, Hiromi |
| description | L-R (left and right) symmetry breaking during embryogenesis and the establishment of asymmetric body plan are key issues in developmental biology, but the onset including the handedness-determining gene locus still remains unknown. Using pure dextral (DD) and sinistral (dd) strains of the pond snail
Lymnaea stagnalis
as well as its F
2
through to F
10
backcrossed lines, the single handedness-determining-gene locus was mapped by genetic linkage analysis, BAC cloning and chromosome walking. We have identified the actin-related diaphanous gene
Lsdia1
as the strongest candidate. Although the cDNA and derived amino acid sequences of the tandemly duplicated
Lsdia1
and
Lsdia2
genes are very similar, we could discriminate the two genes/proteins in our molecular biology experiments. The
Lsdia1
gene of the sinistral strain carries a frameshift mutation that abrogates full-length LsDia1 protein expression. In the dextral strain, it is already translated prior to oviposition. Expression of
Lsdia1
(only in the dextral strain) and
Lsdia2
(in both chirality) decreases after the 1-cell stage, with no asymmetric localization throughout. The evolutionary relationships among body handedness, SD/SI (spiral deformation/spindle inclination) at the third cleavage, and expression of diaphanous proteins are discussed in comparison with three other pond snails (
L. peregra, Physa acuta
and
Indoplanorbis exustus
). |
| doi_str_mv | 10.1038/srep34809 |
| format | Article |
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Lymnaea stagnalis
as well as its F
2
through to F
10
backcrossed lines, the single handedness-determining-gene locus was mapped by genetic linkage analysis, BAC cloning and chromosome walking. We have identified the actin-related diaphanous gene
Lsdia1
as the strongest candidate. Although the cDNA and derived amino acid sequences of the tandemly duplicated
Lsdia1
and
Lsdia2
genes are very similar, we could discriminate the two genes/proteins in our molecular biology experiments. The
Lsdia1
gene of the sinistral strain carries a frameshift mutation that abrogates full-length LsDia1 protein expression. In the dextral strain, it is already translated prior to oviposition. Expression of
Lsdia1
(only in the dextral strain) and
Lsdia2
(in both chirality) decreases after the 1-cell stage, with no asymmetric localization throughout. The evolutionary relationships among body handedness, SD/SI (spiral deformation/spindle inclination) at the third cleavage, and expression of diaphanous proteins are discussed in comparison with three other pond snails (
L. peregra, Physa acuta
and
Indoplanorbis exustus
).</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep34809</identifier><identifier>PMID: 27708420</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38/39 ; 631/136 ; 631/136/1660 ; Actin ; Amino acids ; Amplified Fragment Length Polymorphism Analysis ; Animal Shells - physiology ; Animals ; Bacterial artificial chromosomes ; Body Patterning - genetics ; Chirality ; Chromosome Mapping ; Cloning ; Developmental biology ; Embryo, Nonmammalian - cytology ; Embryogenesis ; Embryonic growth stage ; Female ; Frameshift Mutation ; Gene Expression Regulation, Developmental ; Gene mapping ; Genes ; Handedness ; Humanities and Social Sciences ; Linkage analysis ; Localization ; Lymnaea - embryology ; Lymnaea - genetics ; Lymnaea - physiology ; Male ; Molecular biology ; Mollusks ; multidisciplinary ; Mutation ; Nucleotide sequence ; Oviposition ; Point mutation ; Ponds ; Proteins - genetics ; Proteins - immunology ; Proteins - metabolism ; Science</subject><ispartof>Scientific reports, 2016-10, Vol.6 (1), p.34809-34809, Article 34809</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Nature Publishing Group Oct 2016</rights><rights>Copyright © 2016, The Author(s) 2016 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-de2443953711ff944363377d31eabdcc4502185d62d0f26c0a7139a1fbdfd1963</citedby><cites>FETCH-LOGICAL-c504t-de2443953711ff944363377d31eabdcc4502185d62d0f26c0a7139a1fbdfd1963</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052593/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052593/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27708420$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kuroda, Reiko</creatorcontrib><creatorcontrib>Fujikura, Kohei</creatorcontrib><creatorcontrib>Abe, Masanori</creatorcontrib><creatorcontrib>Hosoiri, Yuji</creatorcontrib><creatorcontrib>Asakawa, Shuichi</creatorcontrib><creatorcontrib>Shimizu, Miho</creatorcontrib><creatorcontrib>Umeda, Shin</creatorcontrib><creatorcontrib>Ichikawa, Futaba</creatorcontrib><creatorcontrib>Takahashi, Hiromi</creatorcontrib><title>Diaphanous gene mutation affects spiral cleavage and chirality in snails</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>L-R (left and right) symmetry breaking during embryogenesis and the establishment of asymmetric body plan are key issues in developmental biology, but the onset including the handedness-determining gene locus still remains unknown. Using pure dextral (DD) and sinistral (dd) strains of the pond snail
Lymnaea stagnalis
as well as its F
2
through to F
10
backcrossed lines, the single handedness-determining-gene locus was mapped by genetic linkage analysis, BAC cloning and chromosome walking. We have identified the actin-related diaphanous gene
Lsdia1
as the strongest candidate. Although the cDNA and derived amino acid sequences of the tandemly duplicated
Lsdia1
and
Lsdia2
genes are very similar, we could discriminate the two genes/proteins in our molecular biology experiments. The
Lsdia1
gene of the sinistral strain carries a frameshift mutation that abrogates full-length LsDia1 protein expression. In the dextral strain, it is already translated prior to oviposition. Expression of
Lsdia1
(only in the dextral strain) and
Lsdia2
(in both chirality) decreases after the 1-cell stage, with no asymmetric localization throughout. The evolutionary relationships among body handedness, SD/SI (spiral deformation/spindle inclination) at the third cleavage, and expression of diaphanous proteins are discussed in comparison with three other pond snails (
L. peregra, Physa acuta
and
Indoplanorbis exustus
).</description><subject>38/39</subject><subject>631/136</subject><subject>631/136/1660</subject><subject>Actin</subject><subject>Amino acids</subject><subject>Amplified Fragment Length Polymorphism Analysis</subject><subject>Animal Shells - physiology</subject><subject>Animals</subject><subject>Bacterial artificial chromosomes</subject><subject>Body Patterning - genetics</subject><subject>Chirality</subject><subject>Chromosome Mapping</subject><subject>Cloning</subject><subject>Developmental biology</subject><subject>Embryo, Nonmammalian - cytology</subject><subject>Embryogenesis</subject><subject>Embryonic growth stage</subject><subject>Female</subject><subject>Frameshift Mutation</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene mapping</subject><subject>Genes</subject><subject>Handedness</subject><subject>Humanities and Social Sciences</subject><subject>Linkage analysis</subject><subject>Localization</subject><subject>Lymnaea - embryology</subject><subject>Lymnaea - genetics</subject><subject>Lymnaea - physiology</subject><subject>Male</subject><subject>Molecular biology</subject><subject>Mollusks</subject><subject>multidisciplinary</subject><subject>Mutation</subject><subject>Nucleotide sequence</subject><subject>Oviposition</subject><subject>Point mutation</subject><subject>Ponds</subject><subject>Proteins - genetics</subject><subject>Proteins - immunology</subject><subject>Proteins - metabolism</subject><subject>Science</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkUtLAzEUhYMottQu_AMScKNCNc-ZyUaQ-oSCG12HNI82ZZoZJzOF_ntTWkvVbHLJ_Tj33BwAzjG6xYgWd7GxNWUFEkegTxDjI0IJOT6oe2AY4wKlw4lgWJyCHslzVDCC-uD10at6rkLVRTizwcJl16rWVwEq56xuI4y1b1QJdWnVSs0sVMFAPd-8-XYNfYAxKF_GM3DiVBntcHcPwOfz08f4dTR5f3kbP0xGmiPWjowljFHBaY6xcyLVGaV5bii2amq0ZhwRXHCTEYMcyTRSOaZCYTc1zmCR0QG43-rW3XRpjbahTVZk3filatayUl7-7gQ_l7NqJXlanwuaBK52Ak311dnYyqWP2palCjb9gsQFTe4YQiyhl3_QRdU1Ia2XKCGQEDzbOLreUrqpYkrD7c1gJDcRyX1Eib04dL8nfwJJwM0WiKkVZrY5GPlP7Rtta5qC</recordid><startdate>20161006</startdate><enddate>20161006</enddate><creator>Kuroda, Reiko</creator><creator>Fujikura, Kohei</creator><creator>Abe, Masanori</creator><creator>Hosoiri, Yuji</creator><creator>Asakawa, Shuichi</creator><creator>Shimizu, Miho</creator><creator>Umeda, Shin</creator><creator>Ichikawa, Futaba</creator><creator>Takahashi, Hiromi</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20161006</creationdate><title>Diaphanous gene mutation affects spiral cleavage and chirality in snails</title><author>Kuroda, Reiko ; Fujikura, Kohei ; Abe, Masanori ; Hosoiri, Yuji ; Asakawa, Shuichi ; Shimizu, Miho ; Umeda, Shin ; Ichikawa, Futaba ; Takahashi, Hiromi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-de2443953711ff944363377d31eabdcc4502185d62d0f26c0a7139a1fbdfd1963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>38/39</topic><topic>631/136</topic><topic>631/136/1660</topic><topic>Actin</topic><topic>Amino acids</topic><topic>Amplified Fragment Length Polymorphism Analysis</topic><topic>Animal Shells - physiology</topic><topic>Animals</topic><topic>Bacterial artificial chromosomes</topic><topic>Body Patterning - genetics</topic><topic>Chirality</topic><topic>Chromosome Mapping</topic><topic>Cloning</topic><topic>Developmental biology</topic><topic>Embryo, Nonmammalian - cytology</topic><topic>Embryogenesis</topic><topic>Embryonic growth stage</topic><topic>Female</topic><topic>Frameshift Mutation</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene mapping</topic><topic>Genes</topic><topic>Handedness</topic><topic>Humanities and Social Sciences</topic><topic>Linkage analysis</topic><topic>Localization</topic><topic>Lymnaea - embryology</topic><topic>Lymnaea - genetics</topic><topic>Lymnaea - physiology</topic><topic>Male</topic><topic>Molecular biology</topic><topic>Mollusks</topic><topic>multidisciplinary</topic><topic>Mutation</topic><topic>Nucleotide sequence</topic><topic>Oviposition</topic><topic>Point mutation</topic><topic>Ponds</topic><topic>Proteins - genetics</topic><topic>Proteins - immunology</topic><topic>Proteins - metabolism</topic><topic>Science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kuroda, Reiko</creatorcontrib><creatorcontrib>Fujikura, Kohei</creatorcontrib><creatorcontrib>Abe, Masanori</creatorcontrib><creatorcontrib>Hosoiri, Yuji</creatorcontrib><creatorcontrib>Asakawa, Shuichi</creatorcontrib><creatorcontrib>Shimizu, Miho</creatorcontrib><creatorcontrib>Umeda, Shin</creatorcontrib><creatorcontrib>Ichikawa, Futaba</creatorcontrib><creatorcontrib>Takahashi, Hiromi</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kuroda, Reiko</au><au>Fujikura, Kohei</au><au>Abe, Masanori</au><au>Hosoiri, Yuji</au><au>Asakawa, Shuichi</au><au>Shimizu, Miho</au><au>Umeda, Shin</au><au>Ichikawa, Futaba</au><au>Takahashi, Hiromi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diaphanous gene mutation affects spiral cleavage and chirality in snails</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-10-06</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>34809</spage><epage>34809</epage><pages>34809-34809</pages><artnum>34809</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>L-R (left and right) symmetry breaking during embryogenesis and the establishment of asymmetric body plan are key issues in developmental biology, but the onset including the handedness-determining gene locus still remains unknown. Using pure dextral (DD) and sinistral (dd) strains of the pond snail
Lymnaea stagnalis
as well as its F
2
through to F
10
backcrossed lines, the single handedness-determining-gene locus was mapped by genetic linkage analysis, BAC cloning and chromosome walking. We have identified the actin-related diaphanous gene
Lsdia1
as the strongest candidate. Although the cDNA and derived amino acid sequences of the tandemly duplicated
Lsdia1
and
Lsdia2
genes are very similar, we could discriminate the two genes/proteins in our molecular biology experiments. The
Lsdia1
gene of the sinistral strain carries a frameshift mutation that abrogates full-length LsDia1 protein expression. In the dextral strain, it is already translated prior to oviposition. Expression of
Lsdia1
(only in the dextral strain) and
Lsdia2
(in both chirality) decreases after the 1-cell stage, with no asymmetric localization throughout. The evolutionary relationships among body handedness, SD/SI (spiral deformation/spindle inclination) at the third cleavage, and expression of diaphanous proteins are discussed in comparison with three other pond snails (
L. peregra, Physa acuta
and
Indoplanorbis exustus
).</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27708420</pmid><doi>10.1038/srep34809</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Scientific reports, 2016-10, Vol.6 (1), p.34809-34809, Article 34809 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; Nature Free; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | 38/39 631/136 631/136/1660 Actin Amino acids Amplified Fragment Length Polymorphism Analysis Animal Shells - physiology Animals Bacterial artificial chromosomes Body Patterning - genetics Chirality Chromosome Mapping Cloning Developmental biology Embryo, Nonmammalian - cytology Embryogenesis Embryonic growth stage Female Frameshift Mutation Gene Expression Regulation, Developmental Gene mapping Genes Handedness Humanities and Social Sciences Linkage analysis Localization Lymnaea - embryology Lymnaea - genetics Lymnaea - physiology Male Molecular biology Mollusks multidisciplinary Mutation Nucleotide sequence Oviposition Point mutation Ponds Proteins - genetics Proteins - immunology Proteins - metabolism Science |
| title | Diaphanous gene mutation affects spiral cleavage and chirality in snails |
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