Cold tolerance and silencing of three cold-tolerance genes of overwintering Chinese white pine larvae
The values of physiological indices and the enzymes activities involved in the overwintering stage were studied in D. armandi larvae in each month from October 2014 to March 2015. The sorbitol, trehalose and glycerol values initially tended to increase as the ambient temperature decreased, before de...
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| description | The values of physiological indices and the enzymes activities involved in the overwintering stage were studied in
D. armandi
larvae in each month from October 2014 to March 2015. The sorbitol, trehalose and glycerol values initially tended to increase as the ambient temperature decreased, before declining until the end of the winter. The activities of four enzymes (SOD, CAT, LDH and AchE) decreased, whereas POD, PK and MDH showed opposite trends in activity. Other enzyme activities (those of TPS, SDH and GLK) were low during the overwintering period and later increased and stabilized during spring. In this study, a polymerase chain reaction (PCR) genes of SDH, TPS and GLK was utilized to identify DarmSDH, DarmTPS and DarmGLK in
D. armandi
. They were found to be abundantly expressed during the overwintering stage by quantitative real-time PCR (qRT-PCR) analyses; by contrast, these three genes showed higher expression levels in December 2014 than in May 2015. The qRT-PCR results demonstrated that the reduction of mRNA expression levels was significant in DarmSDH-, DarmTPS- and DarmGLK-dsRNA-treated
D. armandi
compared with water-injected and non-injected controls. The mortality responses at low temperature were also increased in the dsRNA-treated
D. armandi
compared with the controls. |
| doi_str_mv | 10.1038/srep34698 |
| format | Article |
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D. armandi
larvae in each month from October 2014 to March 2015. The sorbitol, trehalose and glycerol values initially tended to increase as the ambient temperature decreased, before declining until the end of the winter. The activities of four enzymes (SOD, CAT, LDH and AchE) decreased, whereas POD, PK and MDH showed opposite trends in activity. Other enzyme activities (those of TPS, SDH and GLK) were low during the overwintering period and later increased and stabilized during spring. In this study, a polymerase chain reaction (PCR) genes of SDH, TPS and GLK was utilized to identify DarmSDH, DarmTPS and DarmGLK in
D. armandi
. They were found to be abundantly expressed during the overwintering stage by quantitative real-time PCR (qRT-PCR) analyses; by contrast, these three genes showed higher expression levels in December 2014 than in May 2015. The qRT-PCR results demonstrated that the reduction of mRNA expression levels was significant in DarmSDH-, DarmTPS- and DarmGLK-dsRNA-treated
D. armandi
compared with water-injected and non-injected controls. The mortality responses at low temperature were also increased in the dsRNA-treated
D. armandi
compared with the controls.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep34698</identifier><identifier>PMID: 27703270</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38/22 ; 38/39 ; 38/77 ; 38/89 ; 631/337/505 ; 631/601/1466 ; Ambient temperature ; Animals ; Cold Temperature ; Cold tolerance ; Coleoptera - genetics ; Coleoptera - physiology ; Double-stranded RNA ; Enzymatic activity ; Enzymes ; Gene expression ; Gene Expression Profiling - methods ; Gene Expression Regulation ; Gene Silencing ; Glucosyltransferases - genetics ; Glycerol ; Glycerol Kinase - genetics ; Humanities and Social Sciences ; Insect Proteins - genetics ; L-Iditol 2-Dehydrogenase - genetics ; Larva - genetics ; Larva - physiology ; Larvae ; Low temperature ; multidisciplinary ; Overwintering ; Phylogeny ; Pinus - parasitology ; Polymerase chain reaction ; Science ; Seasons ; Sorbitol ; Temperature effects ; Trehalose ; Up-Regulation</subject><ispartof>Scientific reports, 2016-10, Vol.6 (1), p.34698-34698, Article 34698</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Nature Publishing Group Oct 2016</rights><rights>Copyright © 2016, The Author(s) 2016 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-9876e503dd26b9339a86c0218b64e43a695abc87c48ec2128503ecff7ce96c73</citedby><cites>FETCH-LOGICAL-c438t-9876e503dd26b9339a86c0218b64e43a695abc87c48ec2128503ecff7ce96c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5050449/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5050449/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,27907,27908,41103,42172,51559,53774,53776</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27703270$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Juan</creatorcontrib><creatorcontrib>Zhang, Ran-Ran</creatorcontrib><creatorcontrib>Gao, Guan-Qun</creatorcontrib><creatorcontrib>Ma, Ming-Yuan</creatorcontrib><creatorcontrib>Chen, Hui</creatorcontrib><title>Cold tolerance and silencing of three cold-tolerance genes of overwintering Chinese white pine larvae</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>The values of physiological indices and the enzymes activities involved in the overwintering stage were studied in
D. armandi
larvae in each month from October 2014 to March 2015. The sorbitol, trehalose and glycerol values initially tended to increase as the ambient temperature decreased, before declining until the end of the winter. The activities of four enzymes (SOD, CAT, LDH and AchE) decreased, whereas POD, PK and MDH showed opposite trends in activity. Other enzyme activities (those of TPS, SDH and GLK) were low during the overwintering period and later increased and stabilized during spring. In this study, a polymerase chain reaction (PCR) genes of SDH, TPS and GLK was utilized to identify DarmSDH, DarmTPS and DarmGLK in
D. armandi
. They were found to be abundantly expressed during the overwintering stage by quantitative real-time PCR (qRT-PCR) analyses; by contrast, these three genes showed higher expression levels in December 2014 than in May 2015. The qRT-PCR results demonstrated that the reduction of mRNA expression levels was significant in DarmSDH-, DarmTPS- and DarmGLK-dsRNA-treated
D. armandi
compared with water-injected and non-injected controls. The mortality responses at low temperature were also increased in the dsRNA-treated
D. armandi
compared with the controls.</description><subject>38/22</subject><subject>38/39</subject><subject>38/77</subject><subject>38/89</subject><subject>631/337/505</subject><subject>631/601/1466</subject><subject>Ambient temperature</subject><subject>Animals</subject><subject>Cold Temperature</subject><subject>Cold tolerance</subject><subject>Coleoptera - genetics</subject><subject>Coleoptera - physiology</subject><subject>Double-stranded RNA</subject><subject>Enzymatic activity</subject><subject>Enzymes</subject><subject>Gene expression</subject><subject>Gene Expression Profiling - methods</subject><subject>Gene Expression Regulation</subject><subject>Gene Silencing</subject><subject>Glucosyltransferases - genetics</subject><subject>Glycerol</subject><subject>Glycerol Kinase - genetics</subject><subject>Humanities and Social Sciences</subject><subject>Insect Proteins - genetics</subject><subject>L-Iditol 2-Dehydrogenase - genetics</subject><subject>Larva - genetics</subject><subject>Larva - physiology</subject><subject>Larvae</subject><subject>Low temperature</subject><subject>multidisciplinary</subject><subject>Overwintering</subject><subject>Phylogeny</subject><subject>Pinus - parasitology</subject><subject>Polymerase chain reaction</subject><subject>Science</subject><subject>Seasons</subject><subject>Sorbitol</subject><subject>Temperature effects</subject><subject>Trehalose</subject><subject>Up-Regulation</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpl0UtLAzEQAOAgiop68A9IwIsKq3ntbnIRpPgCwYv3kGZn28g2WZNtxX9vSrVWzSWP-ZhMMggdU3JJCZdXKULPRaXkFtpnRJQF44xtb6z30FFKrySPkilB1S7aY3VNOKvJPoJR6Bo8hA6i8Raw8Q1OrgNvnZ_g0OJhGgGwzar4URPwkJbRsID47vwAcclHU5fPAb9P3QC4zxvcmbgwcIh2WtMlOPqaD9DL3e3L6KF4er5_HN08FVZwORRK1hWUhDcNq8aKc2VkZQmjclwJENxUqjRjK2srJFhGmcwWbNvWFlRla36Arldp-_l4Bo0FP0TT6T66mYkfOhinf0e8m-pJWOiSlEQIlROcfSWI4W0OadAzlyx0nfEQ5klTyUsuaclEpqd_6GuYR59fl5VSRClOeVbnK2VjSLlT7boYSvSyfXrdvmxPNqtfy-9mZXCxAqlffjfEjSv_ZfsEy8alLA</recordid><startdate>20161005</startdate><enddate>20161005</enddate><creator>Wang, Juan</creator><creator>Zhang, Ran-Ran</creator><creator>Gao, Guan-Qun</creator><creator>Ma, Ming-Yuan</creator><creator>Chen, Hui</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20161005</creationdate><title>Cold tolerance and silencing of three cold-tolerance genes of overwintering Chinese white pine larvae</title><author>Wang, Juan ; Zhang, Ran-Ran ; Gao, Guan-Qun ; Ma, Ming-Yuan ; Chen, Hui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-9876e503dd26b9339a86c0218b64e43a695abc87c48ec2128503ecff7ce96c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>38/22</topic><topic>38/39</topic><topic>38/77</topic><topic>38/89</topic><topic>631/337/505</topic><topic>631/601/1466</topic><topic>Ambient temperature</topic><topic>Animals</topic><topic>Cold Temperature</topic><topic>Cold tolerance</topic><topic>Coleoptera - genetics</topic><topic>Coleoptera - physiology</topic><topic>Double-stranded RNA</topic><topic>Enzymatic activity</topic><topic>Enzymes</topic><topic>Gene expression</topic><topic>Gene Expression Profiling - methods</topic><topic>Gene Expression Regulation</topic><topic>Gene Silencing</topic><topic>Glucosyltransferases - genetics</topic><topic>Glycerol</topic><topic>Glycerol Kinase - genetics</topic><topic>Humanities and Social Sciences</topic><topic>Insect Proteins - genetics</topic><topic>L-Iditol 2-Dehydrogenase - genetics</topic><topic>Larva - genetics</topic><topic>Larva - physiology</topic><topic>Larvae</topic><topic>Low temperature</topic><topic>multidisciplinary</topic><topic>Overwintering</topic><topic>Phylogeny</topic><topic>Pinus - parasitology</topic><topic>Polymerase chain reaction</topic><topic>Science</topic><topic>Seasons</topic><topic>Sorbitol</topic><topic>Temperature effects</topic><topic>Trehalose</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Juan</creatorcontrib><creatorcontrib>Zhang, Ran-Ran</creatorcontrib><creatorcontrib>Gao, Guan-Qun</creatorcontrib><creatorcontrib>Ma, Ming-Yuan</creatorcontrib><creatorcontrib>Chen, Hui</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Juan</au><au>Zhang, Ran-Ran</au><au>Gao, Guan-Qun</au><au>Ma, Ming-Yuan</au><au>Chen, Hui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cold tolerance and silencing of three cold-tolerance genes of overwintering Chinese white pine larvae</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-10-05</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>34698</spage><epage>34698</epage><pages>34698-34698</pages><artnum>34698</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>The values of physiological indices and the enzymes activities involved in the overwintering stage were studied in
D. armandi
larvae in each month from October 2014 to March 2015. The sorbitol, trehalose and glycerol values initially tended to increase as the ambient temperature decreased, before declining until the end of the winter. The activities of four enzymes (SOD, CAT, LDH and AchE) decreased, whereas POD, PK and MDH showed opposite trends in activity. Other enzyme activities (those of TPS, SDH and GLK) were low during the overwintering period and later increased and stabilized during spring. In this study, a polymerase chain reaction (PCR) genes of SDH, TPS and GLK was utilized to identify DarmSDH, DarmTPS and DarmGLK in
D. armandi
. They were found to be abundantly expressed during the overwintering stage by quantitative real-time PCR (qRT-PCR) analyses; by contrast, these three genes showed higher expression levels in December 2014 than in May 2015. The qRT-PCR results demonstrated that the reduction of mRNA expression levels was significant in DarmSDH-, DarmTPS- and DarmGLK-dsRNA-treated
D. armandi
compared with water-injected and non-injected controls. The mortality responses at low temperature were also increased in the dsRNA-treated
D. armandi
compared with the controls.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27703270</pmid><doi>10.1038/srep34698</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; Nature Free; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 38/22 38/39 38/77 38/89 631/337/505 631/601/1466 Ambient temperature Animals Cold Temperature Cold tolerance Coleoptera - genetics Coleoptera - physiology Double-stranded RNA Enzymatic activity Enzymes Gene expression Gene Expression Profiling - methods Gene Expression Regulation Gene Silencing Glucosyltransferases - genetics Glycerol Glycerol Kinase - genetics Humanities and Social Sciences Insect Proteins - genetics L-Iditol 2-Dehydrogenase - genetics Larva - genetics Larva - physiology Larvae Low temperature multidisciplinary Overwintering Phylogeny Pinus - parasitology Polymerase chain reaction Science Seasons Sorbitol Temperature effects Trehalose Up-Regulation |
| title | Cold tolerance and silencing of three cold-tolerance genes of overwintering Chinese white pine larvae |
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