Effect of an Experimental Direct Pulp-capping Material on the Properties and Osteogenic Differentiation of Human Dental Pulp Stem Cells
Effective pulp-capping materials must have antibacterial properties and induce dentin bridge formation; however, many current materials do not satisfy clinical requirements. Accordingly, the effects of an experiment pulp-capping material (Exp) composed of an antibacterial resin monomer (MAE-DB) and...
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| creator | Yu, Fan Dong, Yan Yang, Yan-wei Lin, Ping-ting Yu, Hao-han Sun, Xiang Sun, Xue-fei Zhou, Huan Huang, Li Chen, Ji-hua |
| description | Effective pulp-capping materials must have antibacterial properties and induce dentin bridge formation; however, many current materials do not satisfy clinical requirements. Accordingly, the effects of an experiment pulp-capping material (Exp) composed of an antibacterial resin monomer (MAE-DB) and Portland cement (PC) on the viability, adhesion, migration, and differentiation of human dental pulp stem cells (hDPSCs) were examined. Based on a Cell Counting Kit-8 assay, hDPSCs exposed to Exp extracts showed limited viability at 24 and 48 h, but displayed comparable viability to the control at 72 h. hDPSC treatment with Exp extracts enhanced cellular adhesion and migration according to
in vitro
scratch wound healing and Transwell migration assays. Exp significantly upregulated the expression of osteogenesis-related genes. The hDPSCs cultured with Exp exhibited higher ALP activity and calcium deposition
in vitro
compared with the control group. The novel material showed comparable cytocompatibility to control cells and promoted the adhesion, migration, and osteogenic differentiation of hDPSCs, indicating excellent biocompatibility. This new direct pulp-capping material containing MAE-DB and PC shows promise as a potential alternative to conventional materials for direct pulp capping. |
| doi_str_mv | 10.1038/srep34713 |
| format | Article |
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in vitro
scratch wound healing and Transwell migration assays. Exp significantly upregulated the expression of osteogenesis-related genes. The hDPSCs cultured with Exp exhibited higher ALP activity and calcium deposition
in vitro
compared with the control group. The novel material showed comparable cytocompatibility to control cells and promoted the adhesion, migration, and osteogenic differentiation of hDPSCs, indicating excellent biocompatibility. This new direct pulp-capping material containing MAE-DB and PC shows promise as a potential alternative to conventional materials for direct pulp capping.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep34713</identifier><identifier>PMID: 27698421</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13 ; 13/100 ; 13/31 ; 14/28 ; 38 ; 38/77 ; 631/154/1438 ; 631/532/1360 ; 639/301/54/990 ; 692/308/2171 ; Adhesion ; Adolescent ; Alkaline Phosphatase - genetics ; Alkaline Phosphatase - metabolism ; Bicuspid - cytology ; Bicuspid - surgery ; Biocompatibility ; Biological Assay ; Calcium ; Cell Adhesion - drug effects ; Cell Differentiation - drug effects ; Cell Survival - drug effects ; Dental Cements - pharmacology ; Dental pulp ; Dental Pulp - cytology ; Dental Pulp - drug effects ; Dental restorative materials ; Dentin ; Extracellular Matrix Proteins - genetics ; Extracellular Matrix Proteins - metabolism ; Gene Expression Regulation - drug effects ; Humanities and Social Sciences ; Humans ; Methacrylates - pharmacology ; Molar - cytology ; Molar - surgery ; multidisciplinary ; Osteoblasts - cytology ; Osteoblasts - drug effects ; Osteocalcin - genetics ; Osteocalcin - metabolism ; Osteogenesis ; Osteogenesis - drug effects ; Osteogenesis - genetics ; Osteonectin - genetics ; Osteonectin - metabolism ; Phosphoproteins - genetics ; Phosphoproteins - metabolism ; Portland cement ; Primary Cell Culture ; Quaternary Ammonium Compounds - pharmacology ; Science ; Sialoglycoproteins - genetics ; Sialoglycoproteins - metabolism ; Stem cells ; Stem Cells - cytology ; Stem Cells - drug effects ; Tooth Extraction ; Wound healing ; Wound Healing - drug effects ; Young Adult</subject><ispartof>Scientific reports, 2016-10, Vol.6 (1), p.34713-34713, Article 34713</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Nature Publishing Group Oct 2016</rights><rights>Copyright © 2016, The Author(s) 2016 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-3472120ca1c7328a0306effcfd95369003c94c8a08317b3e4f9806910bc938e3</citedby><cites>FETCH-LOGICAL-c504t-3472120ca1c7328a0306effcfd95369003c94c8a08317b3e4f9806910bc938e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048176/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5048176/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27698421$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, Fan</creatorcontrib><creatorcontrib>Dong, Yan</creatorcontrib><creatorcontrib>Yang, Yan-wei</creatorcontrib><creatorcontrib>Lin, Ping-ting</creatorcontrib><creatorcontrib>Yu, Hao-han</creatorcontrib><creatorcontrib>Sun, Xiang</creatorcontrib><creatorcontrib>Sun, Xue-fei</creatorcontrib><creatorcontrib>Zhou, Huan</creatorcontrib><creatorcontrib>Huang, Li</creatorcontrib><creatorcontrib>Chen, Ji-hua</creatorcontrib><title>Effect of an Experimental Direct Pulp-capping Material on the Properties and Osteogenic Differentiation of Human Dental Pulp Stem Cells</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Effective pulp-capping materials must have antibacterial properties and induce dentin bridge formation; however, many current materials do not satisfy clinical requirements. Accordingly, the effects of an experiment pulp-capping material (Exp) composed of an antibacterial resin monomer (MAE-DB) and Portland cement (PC) on the viability, adhesion, migration, and differentiation of human dental pulp stem cells (hDPSCs) were examined. Based on a Cell Counting Kit-8 assay, hDPSCs exposed to Exp extracts showed limited viability at 24 and 48 h, but displayed comparable viability to the control at 72 h. hDPSC treatment with Exp extracts enhanced cellular adhesion and migration according to
in vitro
scratch wound healing and Transwell migration assays. Exp significantly upregulated the expression of osteogenesis-related genes. The hDPSCs cultured with Exp exhibited higher ALP activity and calcium deposition
in vitro
compared with the control group. The novel material showed comparable cytocompatibility to control cells and promoted the adhesion, migration, and osteogenic differentiation of hDPSCs, indicating excellent biocompatibility. This new direct pulp-capping material containing MAE-DB and PC shows promise as a potential alternative to conventional materials for direct pulp capping.</description><subject>13</subject><subject>13/100</subject><subject>13/31</subject><subject>14/28</subject><subject>38</subject><subject>38/77</subject><subject>631/154/1438</subject><subject>631/532/1360</subject><subject>639/301/54/990</subject><subject>692/308/2171</subject><subject>Adhesion</subject><subject>Adolescent</subject><subject>Alkaline Phosphatase - genetics</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Bicuspid - cytology</subject><subject>Bicuspid - surgery</subject><subject>Biocompatibility</subject><subject>Biological Assay</subject><subject>Calcium</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Dental Cements - pharmacology</subject><subject>Dental pulp</subject><subject>Dental Pulp - cytology</subject><subject>Dental Pulp - drug effects</subject><subject>Dental restorative materials</subject><subject>Dentin</subject><subject>Extracellular Matrix Proteins - genetics</subject><subject>Extracellular Matrix Proteins - metabolism</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Methacrylates - pharmacology</subject><subject>Molar - cytology</subject><subject>Molar - surgery</subject><subject>multidisciplinary</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - drug effects</subject><subject>Osteocalcin - genetics</subject><subject>Osteocalcin - metabolism</subject><subject>Osteogenesis</subject><subject>Osteogenesis - drug effects</subject><subject>Osteogenesis - genetics</subject><subject>Osteonectin - genetics</subject><subject>Osteonectin - metabolism</subject><subject>Phosphoproteins - genetics</subject><subject>Phosphoproteins - metabolism</subject><subject>Portland cement</subject><subject>Primary Cell Culture</subject><subject>Quaternary Ammonium Compounds - pharmacology</subject><subject>Science</subject><subject>Sialoglycoproteins - genetics</subject><subject>Sialoglycoproteins - metabolism</subject><subject>Stem cells</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - drug effects</subject><subject>Tooth Extraction</subject><subject>Wound healing</subject><subject>Wound Healing - drug effects</subject><subject>Young Adult</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNplkd9qFDEUxoNYbKm98AUk4I0VRvNnMjO5EWS7WqHSgr0P2eyZbcpMMiYZqU_ga3uWrctqc5OQ8zvfdw4fIa84e8-Z7D7kBJOsWy6fkRPBalUJKcTzg_cxOcv5nuFRQtdcvyDHom10Vwt-Qn4v-x5cobGnNtDlwwTJjxCKHeiFT9vKzTxMlbPT5MOGfrMFASzGQMsd0JsUsaN4yNi-pte5QNxA8A67UTihkrfFI40Gl_OIHhc79a0s_V5gpAsYhvySHPV2yHD2eJ-S28_L28VldXX95evi01XlFKtLhYsKLpiz3LVSdJZJ1kDfu36tlWw0Y9Lp2uF_J3m7klD3umON5mzltOxAnpKPO9lpXo2wdjhLsoOZcGmbfplovfm3Evyd2cSfBt073jYo8PZRIMUfM-RiRp8dbmADxDkb3kkllVJCIfrmP_Q-zingdkhpzXQjao3U-Y5yKWbMst8Pw5nZBmz2ASP7-nD6Pfk3TgTe7YCMpbCBdGD5RO0PboOwIg</recordid><startdate>20161004</startdate><enddate>20161004</enddate><creator>Yu, Fan</creator><creator>Dong, Yan</creator><creator>Yang, Yan-wei</creator><creator>Lin, Ping-ting</creator><creator>Yu, Hao-han</creator><creator>Sun, Xiang</creator><creator>Sun, Xue-fei</creator><creator>Zhou, Huan</creator><creator>Huang, Li</creator><creator>Chen, Ji-hua</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20161004</creationdate><title>Effect of an Experimental Direct Pulp-capping Material on the Properties and Osteogenic Differentiation of Human Dental Pulp Stem Cells</title><author>Yu, Fan ; Dong, Yan ; Yang, Yan-wei ; Lin, Ping-ting ; Yu, Hao-han ; Sun, Xiang ; Sun, Xue-fei ; Zhou, Huan ; Huang, Li ; Chen, Ji-hua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-3472120ca1c7328a0306effcfd95369003c94c8a08317b3e4f9806910bc938e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>13</topic><topic>13/100</topic><topic>13/31</topic><topic>14/28</topic><topic>38</topic><topic>38/77</topic><topic>631/154/1438</topic><topic>631/532/1360</topic><topic>639/301/54/990</topic><topic>692/308/2171</topic><topic>Adhesion</topic><topic>Adolescent</topic><topic>Alkaline Phosphatase - genetics</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Bicuspid - cytology</topic><topic>Bicuspid - surgery</topic><topic>Biocompatibility</topic><topic>Biological Assay</topic><topic>Calcium</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Dental Cements - pharmacology</topic><topic>Dental pulp</topic><topic>Dental Pulp - cytology</topic><topic>Dental Pulp - drug effects</topic><topic>Dental restorative materials</topic><topic>Dentin</topic><topic>Extracellular Matrix Proteins - genetics</topic><topic>Extracellular Matrix Proteins - metabolism</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Methacrylates - pharmacology</topic><topic>Molar - cytology</topic><topic>Molar - surgery</topic><topic>multidisciplinary</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - drug effects</topic><topic>Osteocalcin - genetics</topic><topic>Osteocalcin - metabolism</topic><topic>Osteogenesis</topic><topic>Osteogenesis - drug effects</topic><topic>Osteogenesis - genetics</topic><topic>Osteonectin - genetics</topic><topic>Osteonectin - metabolism</topic><topic>Phosphoproteins - genetics</topic><topic>Phosphoproteins - metabolism</topic><topic>Portland cement</topic><topic>Primary Cell Culture</topic><topic>Quaternary Ammonium Compounds - pharmacology</topic><topic>Science</topic><topic>Sialoglycoproteins - genetics</topic><topic>Sialoglycoproteins - metabolism</topic><topic>Stem cells</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - drug effects</topic><topic>Tooth Extraction</topic><topic>Wound healing</topic><topic>Wound Healing - drug effects</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, Fan</creatorcontrib><creatorcontrib>Dong, Yan</creatorcontrib><creatorcontrib>Yang, Yan-wei</creatorcontrib><creatorcontrib>Lin, Ping-ting</creatorcontrib><creatorcontrib>Yu, Hao-han</creatorcontrib><creatorcontrib>Sun, Xiang</creatorcontrib><creatorcontrib>Sun, Xue-fei</creatorcontrib><creatorcontrib>Zhou, Huan</creatorcontrib><creatorcontrib>Huang, Li</creatorcontrib><creatorcontrib>Chen, Ji-hua</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yu, Fan</au><au>Dong, Yan</au><au>Yang, Yan-wei</au><au>Lin, Ping-ting</au><au>Yu, Hao-han</au><au>Sun, Xiang</au><au>Sun, Xue-fei</au><au>Zhou, Huan</au><au>Huang, Li</au><au>Chen, Ji-hua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of an Experimental Direct Pulp-capping Material on the Properties and Osteogenic Differentiation of Human Dental Pulp Stem Cells</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-10-04</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>34713</spage><epage>34713</epage><pages>34713-34713</pages><artnum>34713</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Effective pulp-capping materials must have antibacterial properties and induce dentin bridge formation; however, many current materials do not satisfy clinical requirements. Accordingly, the effects of an experiment pulp-capping material (Exp) composed of an antibacterial resin monomer (MAE-DB) and Portland cement (PC) on the viability, adhesion, migration, and differentiation of human dental pulp stem cells (hDPSCs) were examined. Based on a Cell Counting Kit-8 assay, hDPSCs exposed to Exp extracts showed limited viability at 24 and 48 h, but displayed comparable viability to the control at 72 h. hDPSC treatment with Exp extracts enhanced cellular adhesion and migration according to
in vitro
scratch wound healing and Transwell migration assays. Exp significantly upregulated the expression of osteogenesis-related genes. The hDPSCs cultured with Exp exhibited higher ALP activity and calcium deposition
in vitro
compared with the control group. The novel material showed comparable cytocompatibility to control cells and promoted the adhesion, migration, and osteogenic differentiation of hDPSCs, indicating excellent biocompatibility. This new direct pulp-capping material containing MAE-DB and PC shows promise as a potential alternative to conventional materials for direct pulp capping.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27698421</pmid><doi>10.1038/srep34713</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 13 13/100 13/31 14/28 38 38/77 631/154/1438 631/532/1360 639/301/54/990 692/308/2171 Adhesion Adolescent Alkaline Phosphatase - genetics Alkaline Phosphatase - metabolism Bicuspid - cytology Bicuspid - surgery Biocompatibility Biological Assay Calcium Cell Adhesion - drug effects Cell Differentiation - drug effects Cell Survival - drug effects Dental Cements - pharmacology Dental pulp Dental Pulp - cytology Dental Pulp - drug effects Dental restorative materials Dentin Extracellular Matrix Proteins - genetics Extracellular Matrix Proteins - metabolism Gene Expression Regulation - drug effects Humanities and Social Sciences Humans Methacrylates - pharmacology Molar - cytology Molar - surgery multidisciplinary Osteoblasts - cytology Osteoblasts - drug effects Osteocalcin - genetics Osteocalcin - metabolism Osteogenesis Osteogenesis - drug effects Osteogenesis - genetics Osteonectin - genetics Osteonectin - metabolism Phosphoproteins - genetics Phosphoproteins - metabolism Portland cement Primary Cell Culture Quaternary Ammonium Compounds - pharmacology Science Sialoglycoproteins - genetics Sialoglycoproteins - metabolism Stem cells Stem Cells - cytology Stem Cells - drug effects Tooth Extraction Wound healing Wound Healing - drug effects Young Adult |
| title | Effect of an Experimental Direct Pulp-capping Material on the Properties and Osteogenic Differentiation of Human Dental Pulp Stem Cells |
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