Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery
In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-cryosurgery in patients with colorectal cancer liver metastasis as a reference for understanding the relevance of any changes to the efficacy of cryosurgery. CTC numbers and CTC-related gene expr...
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| Veröffentlicht in: | Cancer biology & therapy 2016-09, Vol.17 (9), p.935-942 |
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| creator | Shi, Jian Li, Yuan Liang, Shuzhen Zeng, Jianying Liu, Guifeng Mu, Feng Li, Haibo Chen, Jibing Liu, Tongjun Niu, Lizhi |
| description | In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-cryosurgery in patients with colorectal cancer liver metastasis as a reference for understanding the relevance of any changes to the efficacy of cryosurgery. CTC numbers and CTC-related gene expression were measured in the peripheral blood of 55 patients with colorectal liver metastasis at 1 day before and 7 and 30 d after cryoablation using magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) combined with real-time quantitative PCR (RT-qPCR). The number of CTCs decreased significantly with postoperative time (P < 0.01). Delta cycle threshold values for the CTC-related genes CEA, Ep-CAM, CK18 and CK19 increased significantly after cryoablation. Furthermore, the expression of CEA, Ep-CAM, CK18 and CK19 decreased significantly with time after cryoablation (P < 0.01). RT-qPCR and FACS combined with MACS has significant diagnostic and prognostic value for evaluating the efficacy of cryosurgery in patients with advanced colorectal cancer. |
| doi_str_mv | 10.1080/15384047.2016.1210731 |
| format | Article |
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CTC numbers and CTC-related gene expression were measured in the peripheral blood of 55 patients with colorectal liver metastasis at 1 day before and 7 and 30 d after cryoablation using magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) combined with real-time quantitative PCR (RT-qPCR). The number of CTCs decreased significantly with postoperative time (P < 0.01). Delta cycle threshold values for the CTC-related genes CEA, Ep-CAM, CK18 and CK19 increased significantly after cryoablation. Furthermore, the expression of CEA, Ep-CAM, CK18 and CK19 decreased significantly with time after cryoablation (P < 0.01). RT-qPCR and FACS combined with MACS has significant diagnostic and prognostic value for evaluating the efficacy of cryosurgery in patients with advanced colorectal cancer.</description><identifier>ISSN: 1538-4047</identifier><identifier>EISSN: 1555-8576</identifier><identifier>DOI: 10.1080/15384047.2016.1210731</identifier><identifier>PMID: 27415969</identifier><language>eng</language><publisher>United States: Taylor & Francis</publisher><subject>Circulating tumor cells ; colorectal cancer ; Colorectal Neoplasms - blood ; Colorectal Neoplasms - genetics ; Colorectal Neoplasms - pathology ; Colorectal Neoplasms - surgery ; cryosurgery ; Cryosurgery - methods ; Female ; flow cytometry ; Flow Cytometry - methods ; Humans ; liver metastasis ; Liver Neoplasms - pathology ; Liver Neoplasms - secondary ; Liver Neoplasms - surgery ; Male ; Neoplasm Metastasis ; Neoplastic Cells, Circulating - pathology ; Prognosis ; Research Paper ; reverse transcriptase polymerase chain reaction</subject><ispartof>Cancer biology & therapy, 2016-09, Vol.17 (9), p.935-942</ispartof><rights>2016 The Author(s). Published with license by Taylor & Francis Group, LLC © Jian Shi, Yuan Li, Shuzhen Liang, Jianying Zeng, Guifeng Liu, Feng Mu, Haibo Li, Jibing Chen, Tongjun Liu, and Lizhi Niu 2016</rights><rights>2016 The Author(s). Published with license by Taylor & Francis Group, LLC 2016 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c534t-9870d9d151717b5944a4afa68311c8801270e32dffe14265b2a94471a1025fba3</citedby><cites>FETCH-LOGICAL-c534t-9870d9d151717b5944a4afa68311c8801270e32dffe14265b2a94471a1025fba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5036405/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5036405/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27415969$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shi, Jian</creatorcontrib><creatorcontrib>Li, Yuan</creatorcontrib><creatorcontrib>Liang, Shuzhen</creatorcontrib><creatorcontrib>Zeng, Jianying</creatorcontrib><creatorcontrib>Liu, Guifeng</creatorcontrib><creatorcontrib>Mu, Feng</creatorcontrib><creatorcontrib>Li, Haibo</creatorcontrib><creatorcontrib>Chen, Jibing</creatorcontrib><creatorcontrib>Liu, Tongjun</creatorcontrib><creatorcontrib>Niu, Lizhi</creatorcontrib><title>Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery</title><title>Cancer biology & therapy</title><addtitle>Cancer Biol Ther</addtitle><description>In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-cryosurgery in patients with colorectal cancer liver metastasis as a reference for understanding the relevance of any changes to the efficacy of cryosurgery. CTC numbers and CTC-related gene expression were measured in the peripheral blood of 55 patients with colorectal liver metastasis at 1 day before and 7 and 30 d after cryoablation using magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) combined with real-time quantitative PCR (RT-qPCR). The number of CTCs decreased significantly with postoperative time (P < 0.01). Delta cycle threshold values for the CTC-related genes CEA, Ep-CAM, CK18 and CK19 increased significantly after cryoablation. Furthermore, the expression of CEA, Ep-CAM, CK18 and CK19 decreased significantly with time after cryoablation (P < 0.01). RT-qPCR and FACS combined with MACS has significant diagnostic and prognostic value for evaluating the efficacy of cryosurgery in patients with advanced colorectal cancer.</description><subject>Circulating tumor cells</subject><subject>colorectal cancer</subject><subject>Colorectal Neoplasms - blood</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Colorectal Neoplasms - surgery</subject><subject>cryosurgery</subject><subject>Cryosurgery - methods</subject><subject>Female</subject><subject>flow cytometry</subject><subject>Flow Cytometry - methods</subject><subject>Humans</subject><subject>liver metastasis</subject><subject>Liver Neoplasms - pathology</subject><subject>Liver Neoplasms - secondary</subject><subject>Liver Neoplasms - surgery</subject><subject>Male</subject><subject>Neoplasm Metastasis</subject><subject>Neoplastic Cells, Circulating - pathology</subject><subject>Prognosis</subject><subject>Research Paper</subject><subject>reverse transcriptase polymerase chain reaction</subject><issn>1538-4047</issn><issn>1555-8576</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>EIF</sourceid><recordid>eNp9kVFv1SAUx4lxcXP6ETQ8-tIrB0ppX4zLsqnJkr3oMzmlcMXQcoV2pt9e6r1b9MWEAAm_8z8n_Ah5A2wHrGXvQYq2ZrXacQbNDjgwJeAZuQApZdVK1Tzf7qKtNuicvMz5B2Nc8aZ7Qc65qkF2TXdB4tWEYc0-0-io8cksAWc_7em8jDFRY0PI1E_UxBCTNTMGanAyNtHgH8o-2hlzWSXgUArtNGfaW1dYitNA0c0FMmmNeUl7m9ZX5MxhyPb16bwk325vvl5_ru7uP325vrqrjBT1XHWtYkM3gAQFqpddXWONDptWAJi2ZcAVs4IPzlmoeSN7joVRgMC4dD2KS_LhmHtY-tEOpgyWMOhD8iOmVUf0-t-XyX_X-_igJRNNzWQJeHcKSPHnYvOsR5-378DJxiVraDkHEN0fVB5Rk2LOybqnNsD0Jks_ytKbLH2SVere_j3jU9WjnQJ8PAJ-Kj864q-YwqBnXIsLl4oGn7X4f4_fYUCmJQ</recordid><startdate>20160901</startdate><enddate>20160901</enddate><creator>Shi, Jian</creator><creator>Li, Yuan</creator><creator>Liang, Shuzhen</creator><creator>Zeng, Jianying</creator><creator>Liu, Guifeng</creator><creator>Mu, Feng</creator><creator>Li, Haibo</creator><creator>Chen, Jibing</creator><creator>Liu, Tongjun</creator><creator>Niu, Lizhi</creator><general>Taylor & Francis</general><scope>0YH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160901</creationdate><title>Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery</title><author>Shi, Jian ; Li, Yuan ; Liang, Shuzhen ; Zeng, Jianying ; Liu, Guifeng ; Mu, Feng ; Li, Haibo ; Chen, Jibing ; Liu, Tongjun ; Niu, Lizhi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c534t-9870d9d151717b5944a4afa68311c8801270e32dffe14265b2a94471a1025fba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Circulating tumor cells</topic><topic>colorectal cancer</topic><topic>Colorectal Neoplasms - blood</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Colorectal Neoplasms - surgery</topic><topic>cryosurgery</topic><topic>Cryosurgery - methods</topic><topic>Female</topic><topic>flow cytometry</topic><topic>Flow Cytometry - methods</topic><topic>Humans</topic><topic>liver metastasis</topic><topic>Liver Neoplasms - pathology</topic><topic>Liver Neoplasms - secondary</topic><topic>Liver Neoplasms - surgery</topic><topic>Male</topic><topic>Neoplasm Metastasis</topic><topic>Neoplastic Cells, Circulating - pathology</topic><topic>Prognosis</topic><topic>Research Paper</topic><topic>reverse transcriptase polymerase chain reaction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shi, Jian</creatorcontrib><creatorcontrib>Li, Yuan</creatorcontrib><creatorcontrib>Liang, Shuzhen</creatorcontrib><creatorcontrib>Zeng, Jianying</creatorcontrib><creatorcontrib>Liu, Guifeng</creatorcontrib><creatorcontrib>Mu, Feng</creatorcontrib><creatorcontrib>Li, Haibo</creatorcontrib><creatorcontrib>Chen, Jibing</creatorcontrib><creatorcontrib>Liu, Tongjun</creatorcontrib><creatorcontrib>Niu, Lizhi</creatorcontrib><collection>Taylor & Francis Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cancer biology & therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shi, Jian</au><au>Li, Yuan</au><au>Liang, Shuzhen</au><au>Zeng, Jianying</au><au>Liu, Guifeng</au><au>Mu, Feng</au><au>Li, Haibo</au><au>Chen, Jibing</au><au>Liu, Tongjun</au><au>Niu, Lizhi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery</atitle><jtitle>Cancer biology & therapy</jtitle><addtitle>Cancer Biol Ther</addtitle><date>2016-09-01</date><risdate>2016</risdate><volume>17</volume><issue>9</issue><spage>935</spage><epage>942</epage><pages>935-942</pages><issn>1538-4047</issn><eissn>1555-8576</eissn><abstract>In this study, we determined the number of peripheral blood circulating tumor cells (CTCs) pre- and post-cryosurgery in patients with colorectal cancer liver metastasis as a reference for understanding the relevance of any changes to the efficacy of cryosurgery. CTC numbers and CTC-related gene expression were measured in the peripheral blood of 55 patients with colorectal liver metastasis at 1 day before and 7 and 30 d after cryoablation using magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS) combined with real-time quantitative PCR (RT-qPCR). The number of CTCs decreased significantly with postoperative time (P < 0.01). Delta cycle threshold values for the CTC-related genes CEA, Ep-CAM, CK18 and CK19 increased significantly after cryoablation. Furthermore, the expression of CEA, Ep-CAM, CK18 and CK19 decreased significantly with time after cryoablation (P < 0.01). 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| subjects | Circulating tumor cells colorectal cancer Colorectal Neoplasms - blood Colorectal Neoplasms - genetics Colorectal Neoplasms - pathology Colorectal Neoplasms - surgery cryosurgery Cryosurgery - methods Female flow cytometry Flow Cytometry - methods Humans liver metastasis Liver Neoplasms - pathology Liver Neoplasms - secondary Liver Neoplasms - surgery Male Neoplasm Metastasis Neoplastic Cells, Circulating - pathology Prognosis Research Paper reverse transcriptase polymerase chain reaction |
| title | Analysis of circulating tumor cells in colorectal cancer liver metastasis patients before and after cryosurgery |
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