Molecularly determined total tumour load in lymph nodes of stage I–II colon cancer patients correlates with high-risk factors. A multicentre prospective study
Stage I–II (pN0) colorectal cancer patients are surgically treated although up to 25 % will eventually die from disease recurrence. Lymph node (LN) status is an independent prognostic factor in colorectal cancer (CRC), and molecular tumour detection in LN of early-stage CRC patients is associated wi...
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| Veröffentlicht in: | Virchows Archiv : an international journal of pathology 2016-10, Vol.469 (4), p.385-394 |
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| creator | Aldecoa, Iban Atares, Begoña Tarragona, Jordi Bernet, Laia Sardon, Jose Domingo Pereda, Teresa Villar, Carlos Mendez, M. Carmen Gonzalez-Obeso, Elvira Elorriaga, Kepa Alonso, Guadalupe Lopez Zamora, Javier Planell, Nuria Palacios, Jose Castells, Antoni Matias-Guiu, Xavier Cuatrecasas, Miriam |
| description | Stage I–II (pN0) colorectal cancer patients are surgically treated although up to 25 % will eventually die from disease recurrence. Lymph node (LN) status is an independent prognostic factor in colorectal cancer (CRC), and molecular tumour detection in LN of early-stage CRC patients is associated with an increased risk of disease recurrence and poor survival. This prospective multicentre study aimed to determine the relationship between LN molecular tumour burden and conventional high-risk factors in stage I–II colon cancer patients. A total of 1940 LN from 149 pathologically assessed pN0 colon cancer patients were analysed for the amount of tumour cytokeratin 19 (CK19) messenger RNA (mRNA) with the quantitative reverse transcription loop-mediated isothermal amplification molecular assay One-Step Nucleic Acid Amplification. Patient’s total tumour load (TTL) resulted from the sum of all CK19 mRNA tumour copies/μL of each positive LN from the colectomy specimen. A median of 15 LN were procured per case (IQR 12;20). Molecular positivity correlated with high-grade (
p
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| doi_str_mv | 10.1007/s00428-016-1990-1 |
| format | Article |
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p
< 0.01), mucinous/signet ring type (
p
= 0.017), male gender (
p
= 0.02), number of collected LN (
p
= 0.012) and total LN weight per case (
p
< 0.01). The TTL was related to pT stage (
p
= 0.01) and tumour size (
p
< 0.01) in low-grade tumours. Multivariate logistic regression showed independent correlation of molecular positivity with gender, tumour grade and number of fresh LN [AUC = 0.71 (95 % CI = 0.62–0.79)]. Our results show that lymph node CK19 mRNA detection correlates with classical high-risk factors in stage I–II colon cancer patients. Total tumour load is a quantitative and objective measure that may help to better stage early colon cancer patients.</description><identifier>ISSN: 0945-6317</identifier><identifier>EISSN: 1432-2307</identifier><identifier>DOI: 10.1007/s00428-016-1990-1</identifier><identifier>PMID: 27447172</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Aged ; Colonic Neoplasms - diagnosis ; Colonic Neoplasms - pathology ; Colorectal carcinoma ; Female ; Health risks ; Humans ; Lymph nodes ; Lymph Nodes - pathology ; Lymphatic Metastasis ; Male ; Medicine ; Medicine & Public Health ; Middle Aged ; Neoplasm Recurrence, Local - diagnosis ; Neoplasm Recurrence, Local - pathology ; Neoplasm Staging - methods ; Nucleic acids ; Original ; Original Article ; Pathology ; Prospective Studies ; Risk Factors ; Tumor Burden ; Tumors</subject><ispartof>Virchows Archiv : an international journal of pathology, 2016-10, Vol.469 (4), p.385-394</ispartof><rights>The Author(s) 2016</rights><rights>Springer-Verlag Berlin Heidelberg 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-a3cd42d7ac45a0a41c5a985267412cddd02a38b3d54ccf315c8fc3befbf5d6113</citedby><cites>FETCH-LOGICAL-c470t-a3cd42d7ac45a0a41c5a985267412cddd02a38b3d54ccf315c8fc3befbf5d6113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00428-016-1990-1$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00428-016-1990-1$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,315,781,785,886,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27447172$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aldecoa, Iban</creatorcontrib><creatorcontrib>Atares, Begoña</creatorcontrib><creatorcontrib>Tarragona, Jordi</creatorcontrib><creatorcontrib>Bernet, Laia</creatorcontrib><creatorcontrib>Sardon, Jose Domingo</creatorcontrib><creatorcontrib>Pereda, Teresa</creatorcontrib><creatorcontrib>Villar, Carlos</creatorcontrib><creatorcontrib>Mendez, M. Carmen</creatorcontrib><creatorcontrib>Gonzalez-Obeso, Elvira</creatorcontrib><creatorcontrib>Elorriaga, Kepa</creatorcontrib><creatorcontrib>Alonso, Guadalupe Lopez</creatorcontrib><creatorcontrib>Zamora, Javier</creatorcontrib><creatorcontrib>Planell, Nuria</creatorcontrib><creatorcontrib>Palacios, Jose</creatorcontrib><creatorcontrib>Castells, Antoni</creatorcontrib><creatorcontrib>Matias-Guiu, Xavier</creatorcontrib><creatorcontrib>Cuatrecasas, Miriam</creatorcontrib><title>Molecularly determined total tumour load in lymph nodes of stage I–II colon cancer patients correlates with high-risk factors. A multicentre prospective study</title><title>Virchows Archiv : an international journal of pathology</title><addtitle>Virchows Arch</addtitle><addtitle>Virchows Arch</addtitle><description>Stage I–II (pN0) colorectal cancer patients are surgically treated although up to 25 % will eventually die from disease recurrence. Lymph node (LN) status is an independent prognostic factor in colorectal cancer (CRC), and molecular tumour detection in LN of early-stage CRC patients is associated with an increased risk of disease recurrence and poor survival. This prospective multicentre study aimed to determine the relationship between LN molecular tumour burden and conventional high-risk factors in stage I–II colon cancer patients. A total of 1940 LN from 149 pathologically assessed pN0 colon cancer patients were analysed for the amount of tumour cytokeratin 19 (CK19) messenger RNA (mRNA) with the quantitative reverse transcription loop-mediated isothermal amplification molecular assay One-Step Nucleic Acid Amplification. Patient’s total tumour load (TTL) resulted from the sum of all CK19 mRNA tumour copies/μL of each positive LN from the colectomy specimen. A median of 15 LN were procured per case (IQR 12;20). Molecular positivity correlated with high-grade (
p
< 0.01), mucinous/signet ring type (
p
= 0.017), male gender (
p
= 0.02), number of collected LN (
p
= 0.012) and total LN weight per case (
p
< 0.01). The TTL was related to pT stage (
p
= 0.01) and tumour size (
p
< 0.01) in low-grade tumours. Multivariate logistic regression showed independent correlation of molecular positivity with gender, tumour grade and number of fresh LN [AUC = 0.71 (95 % CI = 0.62–0.79)]. Our results show that lymph node CK19 mRNA detection correlates with classical high-risk factors in stage I–II colon cancer patients. Total tumour load is a quantitative and objective measure that may help to better stage early colon cancer patients.</description><subject>Aged</subject><subject>Colonic Neoplasms - diagnosis</subject><subject>Colonic Neoplasms - pathology</subject><subject>Colorectal carcinoma</subject><subject>Female</subject><subject>Health risks</subject><subject>Humans</subject><subject>Lymph nodes</subject><subject>Lymph Nodes - pathology</subject><subject>Lymphatic Metastasis</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Neoplasm Recurrence, Local - diagnosis</subject><subject>Neoplasm Recurrence, Local - pathology</subject><subject>Neoplasm Staging - methods</subject><subject>Nucleic acids</subject><subject>Original</subject><subject>Original Article</subject><subject>Pathology</subject><subject>Prospective Studies</subject><subject>Risk Factors</subject><subject>Tumor Burden</subject><subject>Tumors</subject><issn>0945-6317</issn><issn>1432-2307</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kU1uFDEQhS0EIsPAAdggS2zYdPBf_22QoojASEFsYG157OppB7fd2O6g2XGHXICzcRI8TIgCEitLVV8916uH0HNKTikh7etEiGBdRWhT0b4nFX2AVlRwVjFO2odoRXpRVw2n7Ql6ktIVIYx2tHmMTlgrREtbtkI_PgQHenEquj02kCFO1oPBOWTlcF6msETsgjLYeuz20zxiHwwkHAacstoB3vz8frPZYB1c8FgrryHiWWULPqdSjRGcymXgm80jHu1urKJNX_CgdA4xneIzPC0uW134CHiOIc2gs72Gor-Y_VP0aFAuwbPbd40-X7z9dP6-uvz4bnN-dllp0ZJcKa6NYKZVWtSKKEF1rfquZk0rKNPGGMIU77bc1ELrgdNad4PmWxi2Q20aSvkavTnqzst2AvN7HeXkHO2k4l4GZeXfHW9HuQvXsiac931bBF7dCsTwdYGU5WSTBueUh7AkSbuSCu9EzQr68h_0qpzZF3sHinHeNAVdI3qkdLlJijDcLUOJPOQvj_nLkr885C8PLl7cd3E38SfwArAjkErL7yDe-_q_qr8AyIbA3A</recordid><startdate>20161001</startdate><enddate>20161001</enddate><creator>Aldecoa, Iban</creator><creator>Atares, Begoña</creator><creator>Tarragona, Jordi</creator><creator>Bernet, Laia</creator><creator>Sardon, Jose Domingo</creator><creator>Pereda, Teresa</creator><creator>Villar, Carlos</creator><creator>Mendez, M. 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A multicentre prospective study</title><author>Aldecoa, Iban ; Atares, Begoña ; Tarragona, Jordi ; Bernet, Laia ; Sardon, Jose Domingo ; Pereda, Teresa ; Villar, Carlos ; Mendez, M. Carmen ; Gonzalez-Obeso, Elvira ; Elorriaga, Kepa ; Alonso, Guadalupe Lopez ; Zamora, Javier ; Planell, Nuria ; Palacios, Jose ; Castells, Antoni ; Matias-Guiu, Xavier ; Cuatrecasas, Miriam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-a3cd42d7ac45a0a41c5a985267412cddd02a38b3d54ccf315c8fc3befbf5d6113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Aged</topic><topic>Colonic Neoplasms - diagnosis</topic><topic>Colonic Neoplasms - pathology</topic><topic>Colorectal carcinoma</topic><topic>Female</topic><topic>Health risks</topic><topic>Humans</topic><topic>Lymph nodes</topic><topic>Lymph Nodes - pathology</topic><topic>Lymphatic Metastasis</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Neoplasm Recurrence, Local - diagnosis</topic><topic>Neoplasm Recurrence, Local - pathology</topic><topic>Neoplasm Staging - methods</topic><topic>Nucleic acids</topic><topic>Original</topic><topic>Original Article</topic><topic>Pathology</topic><topic>Prospective Studies</topic><topic>Risk Factors</topic><topic>Tumor Burden</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aldecoa, Iban</creatorcontrib><creatorcontrib>Atares, Begoña</creatorcontrib><creatorcontrib>Tarragona, Jordi</creatorcontrib><creatorcontrib>Bernet, Laia</creatorcontrib><creatorcontrib>Sardon, Jose Domingo</creatorcontrib><creatorcontrib>Pereda, Teresa</creatorcontrib><creatorcontrib>Villar, Carlos</creatorcontrib><creatorcontrib>Mendez, M. 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Carmen</au><au>Gonzalez-Obeso, Elvira</au><au>Elorriaga, Kepa</au><au>Alonso, Guadalupe Lopez</au><au>Zamora, Javier</au><au>Planell, Nuria</au><au>Palacios, Jose</au><au>Castells, Antoni</au><au>Matias-Guiu, Xavier</au><au>Cuatrecasas, Miriam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecularly determined total tumour load in lymph nodes of stage I–II colon cancer patients correlates with high-risk factors. A multicentre prospective study</atitle><jtitle>Virchows Archiv : an international journal of pathology</jtitle><stitle>Virchows Arch</stitle><addtitle>Virchows Arch</addtitle><date>2016-10-01</date><risdate>2016</risdate><volume>469</volume><issue>4</issue><spage>385</spage><epage>394</epage><pages>385-394</pages><issn>0945-6317</issn><eissn>1432-2307</eissn><abstract>Stage I–II (pN0) colorectal cancer patients are surgically treated although up to 25 % will eventually die from disease recurrence. Lymph node (LN) status is an independent prognostic factor in colorectal cancer (CRC), and molecular tumour detection in LN of early-stage CRC patients is associated with an increased risk of disease recurrence and poor survival. This prospective multicentre study aimed to determine the relationship between LN molecular tumour burden and conventional high-risk factors in stage I–II colon cancer patients. A total of 1940 LN from 149 pathologically assessed pN0 colon cancer patients were analysed for the amount of tumour cytokeratin 19 (CK19) messenger RNA (mRNA) with the quantitative reverse transcription loop-mediated isothermal amplification molecular assay One-Step Nucleic Acid Amplification. Patient’s total tumour load (TTL) resulted from the sum of all CK19 mRNA tumour copies/μL of each positive LN from the colectomy specimen. A median of 15 LN were procured per case (IQR 12;20). Molecular positivity correlated with high-grade (
p
< 0.01), mucinous/signet ring type (
p
= 0.017), male gender (
p
= 0.02), number of collected LN (
p
= 0.012) and total LN weight per case (
p
< 0.01). The TTL was related to pT stage (
p
= 0.01) and tumour size (
p
< 0.01) in low-grade tumours. Multivariate logistic regression showed independent correlation of molecular positivity with gender, tumour grade and number of fresh LN [AUC = 0.71 (95 % CI = 0.62–0.79)]. Our results show that lymph node CK19 mRNA detection correlates with classical high-risk factors in stage I–II colon cancer patients. Total tumour load is a quantitative and objective measure that may help to better stage early colon cancer patients.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>27447172</pmid><doi>10.1007/s00428-016-1990-1</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Virchows Archiv : an international journal of pathology, 2016-10, Vol.469 (4), p.385-394 |
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| source | MEDLINE; Springer Online Journals Complete |
| subjects | Aged Colonic Neoplasms - diagnosis Colonic Neoplasms - pathology Colorectal carcinoma Female Health risks Humans Lymph nodes Lymph Nodes - pathology Lymphatic Metastasis Male Medicine Medicine & Public Health Middle Aged Neoplasm Recurrence, Local - diagnosis Neoplasm Recurrence, Local - pathology Neoplasm Staging - methods Nucleic acids Original Original Article Pathology Prospective Studies Risk Factors Tumor Burden Tumors |
| title | Molecularly determined total tumour load in lymph nodes of stage I–II colon cancer patients correlates with high-risk factors. A multicentre prospective study |
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