Quantitative definition and monitoring of the host cell protein proteome using iTRAQ - a study of an industrial mAb producing CHO-S cell line

There are few studies defining CHO host cell proteins (HCPs) and the flux of these throughout a downstream purification process. Here we have applied quantitative iTRAQ proteomics to follow the HCP profile of an antibody (mAb) producing CHO‐S cell line throughout a standard downstream purification p...

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Veröffentlicht in:	Biotechnology journal 2016-08, Vol.11 (8), p.1014-1024
Hauptverfasser:	Chiverton, Lesley M., Evans, Caroline, Pandhal, Jagroop, Landels, Andrew R., Rees, Byron J., Levison, Peter R., Wright, Phillip C., Smales, C. Mark
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - isolation & purification Bioprocessing Chinese hamster ovary (CHO) CHO Cells Chromatography, Ion Exchange Cricetinae Cricetulus Host cell proteins Industrial Microbiology - methods iTRAQ Monoclonal antibody Proteomics - methods Staphylococcal Protein A - analysis
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