Molecular imaging of brain localization of liposomes in mice using MALDI mass spectrometry

Phospholipids have excellent biocompatibility and are therefore often used as main components of liposomal drug carriers. In traditional bioanalytics, the in-vivo distribution of liposomal drug carriers is assessed using radiolabeled liposomal constituents. This study presents matrix-assisted laser...

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Veröffentlicht in:Scientific reports 2016-09, Vol.6 (1), p.33791-33791, Article 33791
Hauptverfasser: Fülöp, Annabelle, Sammour, Denis A., Erich, Katrin, von Gerichten, Johanna, van Hoogevest, Peter, Sandhoff, Roger, Hopf, Carsten
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container_title Scientific reports
container_volume 6
creator Fülöp, Annabelle
Sammour, Denis A.
Erich, Katrin
von Gerichten, Johanna
van Hoogevest, Peter
Sandhoff, Roger
Hopf, Carsten
description Phospholipids have excellent biocompatibility and are therefore often used as main components of liposomal drug carriers. In traditional bioanalytics, the in-vivo distribution of liposomal drug carriers is assessed using radiolabeled liposomal constituents. This study presents matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) as an alternative, label-free method for ex-vivo molecular imaging of liposomal drug carriers in mouse tissue. To this end, indocyanine green as cargo and two liposomal markers, 1,2-dipalmitoyl- sn -glycero-3-phosphoglycerol (DPPG) and 1,2-distearoyl- sn -glycero-3-phosphoethanolamine conjugated with monodisperse polyethylene glycol (PEG 36 -DSPE) were incorporated into liposomal carriers and administered to mice. We used MALDI MSI of the two lipid markers in both positive and negative ion mode for visualization of liposome integrity and distribution in mouse organs. Additional MSI of hemoglobin in the same tissue slice and pixel-by-pixel computational analysis of co-occurrence of lipid markers and hemoglobin served as indicator of liposome localization either in parenchyma or in blood vessels. Our proof-of-concept study suggests that liposomal components and indocyanine green distributed into all investigated organs.
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subjects 631/154/152
631/92/152
631/92/287/1194
Biocompatibility
Blood vessels
Computational neuroscience
Computer applications
Desorption
Drug delivery
Hemoglobin
Humanities and Social Sciences
Ionization
Ions
Lasers
Liposomes
Localization
Mass spectrometry
Mass spectroscopy
multidisciplinary
Neuroimaging
Parenchyma
Phospholipids
Polyethylene glycol
Science
Scientific imaging
title Molecular imaging of brain localization of liposomes in mice using MALDI mass spectrometry
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