Molecular imaging of brain localization of liposomes in mice using MALDI mass spectrometry
Phospholipids have excellent biocompatibility and are therefore often used as main components of liposomal drug carriers. In traditional bioanalytics, the in-vivo distribution of liposomal drug carriers is assessed using radiolabeled liposomal constituents. This study presents matrix-assisted laser...
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| Veröffentlicht in: | Scientific reports 2016-09, Vol.6 (1), p.33791-33791, Article 33791 |
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| description | Phospholipids have excellent biocompatibility and are therefore often used as main components of liposomal drug carriers. In traditional bioanalytics, the
in-vivo
distribution of liposomal drug carriers is assessed using radiolabeled liposomal constituents. This study presents matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) as an alternative, label-free method for
ex-vivo
molecular imaging of liposomal drug carriers in mouse tissue. To this end, indocyanine green as cargo and two liposomal markers, 1,2-dipalmitoyl-
sn
-glycero-3-phosphoglycerol (DPPG) and 1,2-distearoyl-
sn
-glycero-3-phosphoethanolamine conjugated with monodisperse polyethylene glycol (PEG
36
-DSPE) were incorporated into liposomal carriers and administered to mice. We used MALDI MSI of the two lipid markers in both positive and negative ion mode for visualization of liposome integrity and distribution in mouse organs. Additional MSI of hemoglobin in the same tissue slice and pixel-by-pixel computational analysis of co-occurrence of lipid markers and hemoglobin served as indicator of liposome localization either in parenchyma or in blood vessels. Our proof-of-concept study suggests that liposomal components and indocyanine green distributed into all investigated organs. |
| doi_str_mv | 10.1038/srep33791 |
| format | Article |
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in-vivo
distribution of liposomal drug carriers is assessed using radiolabeled liposomal constituents. This study presents matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) as an alternative, label-free method for
ex-vivo
molecular imaging of liposomal drug carriers in mouse tissue. To this end, indocyanine green as cargo and two liposomal markers, 1,2-dipalmitoyl-
sn
-glycero-3-phosphoglycerol (DPPG) and 1,2-distearoyl-
sn
-glycero-3-phosphoethanolamine conjugated with monodisperse polyethylene glycol (PEG
36
-DSPE) were incorporated into liposomal carriers and administered to mice. We used MALDI MSI of the two lipid markers in both positive and negative ion mode for visualization of liposome integrity and distribution in mouse organs. Additional MSI of hemoglobin in the same tissue slice and pixel-by-pixel computational analysis of co-occurrence of lipid markers and hemoglobin served as indicator of liposome localization either in parenchyma or in blood vessels. Our proof-of-concept study suggests that liposomal components and indocyanine green distributed into all investigated organs.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep33791</identifier><identifier>PMID: 27650487</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/154/152 ; 631/92/152 ; 631/92/287/1194 ; Biocompatibility ; Blood vessels ; Computational neuroscience ; Computer applications ; Desorption ; Drug delivery ; Hemoglobin ; Humanities and Social Sciences ; Ionization ; Ions ; Lasers ; Liposomes ; Localization ; Mass spectrometry ; Mass spectroscopy ; multidisciplinary ; Neuroimaging ; Parenchyma ; Phospholipids ; Polyethylene glycol ; Science ; Scientific imaging</subject><ispartof>Scientific reports, 2016-09, Vol.6 (1), p.33791-33791, Article 33791</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Nature Publishing Group Sep 2016</rights><rights>Copyright © 2016, The Author(s) 2016 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-ff383c3b08cc24978f4dada6a04e7ff25b6a6b85ff84dde47af2ac7f64c841243</citedby><cites>FETCH-LOGICAL-c504t-ff383c3b08cc24978f4dada6a04e7ff25b6a6b85ff84dde47af2ac7f64c841243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030664/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5030664/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,27931,27932,41127,42196,51583,53798,53800</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27650487$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fülöp, Annabelle</creatorcontrib><creatorcontrib>Sammour, Denis A.</creatorcontrib><creatorcontrib>Erich, Katrin</creatorcontrib><creatorcontrib>von Gerichten, Johanna</creatorcontrib><creatorcontrib>van Hoogevest, Peter</creatorcontrib><creatorcontrib>Sandhoff, Roger</creatorcontrib><creatorcontrib>Hopf, Carsten</creatorcontrib><title>Molecular imaging of brain localization of liposomes in mice using MALDI mass spectrometry</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Phospholipids have excellent biocompatibility and are therefore often used as main components of liposomal drug carriers. In traditional bioanalytics, the
in-vivo
distribution of liposomal drug carriers is assessed using radiolabeled liposomal constituents. This study presents matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) as an alternative, label-free method for
ex-vivo
molecular imaging of liposomal drug carriers in mouse tissue. To this end, indocyanine green as cargo and two liposomal markers, 1,2-dipalmitoyl-
sn
-glycero-3-phosphoglycerol (DPPG) and 1,2-distearoyl-
sn
-glycero-3-phosphoethanolamine conjugated with monodisperse polyethylene glycol (PEG
36
-DSPE) were incorporated into liposomal carriers and administered to mice. We used MALDI MSI of the two lipid markers in both positive and negative ion mode for visualization of liposome integrity and distribution in mouse organs. Additional MSI of hemoglobin in the same tissue slice and pixel-by-pixel computational analysis of co-occurrence of lipid markers and hemoglobin served as indicator of liposome localization either in parenchyma or in blood vessels. Our proof-of-concept study suggests that liposomal components and indocyanine green distributed into all investigated organs.</description><subject>631/154/152</subject><subject>631/92/152</subject><subject>631/92/287/1194</subject><subject>Biocompatibility</subject><subject>Blood vessels</subject><subject>Computational neuroscience</subject><subject>Computer applications</subject><subject>Desorption</subject><subject>Drug delivery</subject><subject>Hemoglobin</subject><subject>Humanities and Social Sciences</subject><subject>Ionization</subject><subject>Ions</subject><subject>Lasers</subject><subject>Liposomes</subject><subject>Localization</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>multidisciplinary</subject><subject>Neuroimaging</subject><subject>Parenchyma</subject><subject>Phospholipids</subject><subject>Polyethylene glycol</subject><subject>Science</subject><subject>Scientific imaging</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkc9LBCEcxSWKiurQPxADXSrYctRR5xIs_YaNLnXpIo6jm-GMk84E9dfnsLVs5UXxfXy-Lw-A_Rye5hDzsxh0hzEr8zWwjSApJggjtL5y3gJ7Mb7CtApUkrzcBFuI0QISzrbB8713Wg1Ohsw2cm7beeZNVgVp28x5JZ39lL317XjrbOejb3TMkthYpbMhjg_up7PLu6yRMWax06oPienDxy7YMNJFvfe974Cn66vHi9vJ7OHm7mI6m6iUoZ8YgzlWuIJcKURKxg2pZS2phEQzY1BRUUkrXhjDSV1rwqRBUjFDieIkRwTvgPOFbzdUja6VbvsgnehCGih8CC-t-K209kXM_bsoIIaUjgZH3wbBvw069qKxUWnnZKv9EEXOESKUlnBED_-gr34IbRovUWUJOc8ZS9TxglLBx9SPWYbJoRhLE8vSEnuwmn5J_lSUgJMFEJPUznVY-fKf2xd_fqJT</recordid><startdate>20160921</startdate><enddate>20160921</enddate><creator>Fülöp, Annabelle</creator><creator>Sammour, Denis A.</creator><creator>Erich, Katrin</creator><creator>von Gerichten, Johanna</creator><creator>van Hoogevest, Peter</creator><creator>Sandhoff, Roger</creator><creator>Hopf, Carsten</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160921</creationdate><title>Molecular imaging of brain localization of liposomes in mice using MALDI mass spectrometry</title><author>Fülöp, Annabelle ; Sammour, Denis A. ; Erich, Katrin ; von Gerichten, Johanna ; van Hoogevest, Peter ; Sandhoff, Roger ; Hopf, Carsten</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-ff383c3b08cc24978f4dada6a04e7ff25b6a6b85ff84dde47af2ac7f64c841243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>631/154/152</topic><topic>631/92/152</topic><topic>631/92/287/1194</topic><topic>Biocompatibility</topic><topic>Blood vessels</topic><topic>Computational neuroscience</topic><topic>Computer applications</topic><topic>Desorption</topic><topic>Drug delivery</topic><topic>Hemoglobin</topic><topic>Humanities and Social Sciences</topic><topic>Ionization</topic><topic>Ions</topic><topic>Lasers</topic><topic>Liposomes</topic><topic>Localization</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>multidisciplinary</topic><topic>Neuroimaging</topic><topic>Parenchyma</topic><topic>Phospholipids</topic><topic>Polyethylene glycol</topic><topic>Science</topic><topic>Scientific imaging</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fülöp, Annabelle</creatorcontrib><creatorcontrib>Sammour, Denis A.</creatorcontrib><creatorcontrib>Erich, Katrin</creatorcontrib><creatorcontrib>von Gerichten, Johanna</creatorcontrib><creatorcontrib>van Hoogevest, Peter</creatorcontrib><creatorcontrib>Sandhoff, Roger</creatorcontrib><creatorcontrib>Hopf, Carsten</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database (ProQuest)</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fülöp, Annabelle</au><au>Sammour, Denis A.</au><au>Erich, Katrin</au><au>von Gerichten, Johanna</au><au>van Hoogevest, Peter</au><au>Sandhoff, Roger</au><au>Hopf, Carsten</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular imaging of brain localization of liposomes in mice using MALDI mass spectrometry</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-09-21</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>33791</spage><epage>33791</epage><pages>33791-33791</pages><artnum>33791</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Phospholipids have excellent biocompatibility and are therefore often used as main components of liposomal drug carriers. In traditional bioanalytics, the
in-vivo
distribution of liposomal drug carriers is assessed using radiolabeled liposomal constituents. This study presents matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) as an alternative, label-free method for
ex-vivo
molecular imaging of liposomal drug carriers in mouse tissue. To this end, indocyanine green as cargo and two liposomal markers, 1,2-dipalmitoyl-
sn
-glycero-3-phosphoglycerol (DPPG) and 1,2-distearoyl-
sn
-glycero-3-phosphoethanolamine conjugated with monodisperse polyethylene glycol (PEG
36
-DSPE) were incorporated into liposomal carriers and administered to mice. We used MALDI MSI of the two lipid markers in both positive and negative ion mode for visualization of liposome integrity and distribution in mouse organs. Additional MSI of hemoglobin in the same tissue slice and pixel-by-pixel computational analysis of co-occurrence of lipid markers and hemoglobin served as indicator of liposome localization either in parenchyma or in blood vessels. Our proof-of-concept study suggests that liposomal components and indocyanine green distributed into all investigated organs.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27650487</pmid><doi>10.1038/srep33791</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/154/152 631/92/152 631/92/287/1194 Biocompatibility Blood vessels Computational neuroscience Computer applications Desorption Drug delivery Hemoglobin Humanities and Social Sciences Ionization Ions Lasers Liposomes Localization Mass spectrometry Mass spectroscopy multidisciplinary Neuroimaging Parenchyma Phospholipids Polyethylene glycol Science Scientific imaging |
| title | Molecular imaging of brain localization of liposomes in mice using MALDI mass spectrometry |
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