Epigenetics changes caused by the fusion of human embryonic stem cell and ovarian cancer cells
To observe the effect of gene expression and tumorigenicity in hybrid cells of human embryonic stem cells (hESCs) and ovarian cancer cells in vitro and in vivo using a mouse model, and to determine its feasibility in reprogramming tumour cells growth and apoptosis, for a potential exploration of the...
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| Veröffentlicht in: | Bioscience reports 2016-10, Vol.36 (5), p.e00378-e00378 |
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| creator | He, Ke Qu, Hu Xu, Li-Nan Gao, Jun Cheng, Fu-Yi Xiang, Peng Zhou, Can-Quan |
| description | To observe the effect of gene expression and tumorigenicity in hybrid cells of human embryonic stem cells (hESCs) and ovarian cancer cells in vitro and in vivo using a mouse model, and to determine its feasibility in reprogramming tumour cells growth and apoptosis, for a potential exploration of the role of hESCs and tumour cells fusion in the management of ovarian cancer. Stable transgenic hESCs (H1) and ovarian cancer cell line OVCAR-3 were established before fusion, and cell fusion system was established to analyse the related indicators. PTEN expression in HO-H1 cells was higher than those in the parental stem cells and lower than those in parental tumour cells; the growth of OV-H1 (RFP+GFP) hybrid cells with double fluorescence expressions were obviously slower than that of human embryonic stem cells and OVCAR-3 ovarian cancer cells. The apoptosis signal of the OV-H1 hybrid cells was significantly higher than that of the hESCs and OVCAR-3 ovarian cancer cells. In vivo results showed that compared with 7 days, 28 days and 35 days after inoculation of OV-H1 hybrid cells; also, apoptotic cell detection indicated that much stronger apoptotic signal was found in OV-H1 hybrid cells inoculated mouse. The hESCs can inhibit the growth of OVCAR-3 cells in vitro by suppressing p53 and PTEN expression to suppress the growth of tumour that may be achieved by inducing apoptosis of OVCAR-3 cells. The change of epigenetics after fusion of ovarian cancer cells and hESCs may become a novel direction for treatment of ovarian cancer. |
| doi_str_mv | 10.1042/BSR20160104 |
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Stable transgenic hESCs (H1) and ovarian cancer cell line OVCAR-3 were established before fusion, and cell fusion system was established to analyse the related indicators. PTEN expression in HO-H1 cells was higher than those in the parental stem cells and lower than those in parental tumour cells; the growth of OV-H1 (RFP+GFP) hybrid cells with double fluorescence expressions were obviously slower than that of human embryonic stem cells and OVCAR-3 ovarian cancer cells. The apoptosis signal of the OV-H1 hybrid cells was significantly higher than that of the hESCs and OVCAR-3 ovarian cancer cells. In vivo results showed that compared with 7 days, 28 days and 35 days after inoculation of OV-H1 hybrid cells; also, apoptotic cell detection indicated that much stronger apoptotic signal was found in OV-H1 hybrid cells inoculated mouse. The hESCs can inhibit the growth of OVCAR-3 cells in vitro by suppressing p53 and PTEN expression to suppress the growth of tumour that may be achieved by inducing apoptosis of OVCAR-3 cells. The change of epigenetics after fusion of ovarian cancer cells and hESCs may become a novel direction for treatment of ovarian cancer.</description><identifier>ISSN: 0144-8463</identifier><identifier>EISSN: 1573-4935</identifier><identifier>DOI: 10.1042/BSR20160104</identifier><identifier>PMID: 27377320</identifier><language>eng</language><publisher>England: Portland Press Ltd</publisher><subject>Animals ; Apoptosis - genetics ; Cell Fusion ; Cell Line, Tumor ; Cell Proliferation - genetics ; Drug Resistance, Neoplasm ; Epigenesis, Genetic ; Female ; Gene Expression Regulation, Neoplastic ; Human Embryonic Stem Cells - cytology ; Human Embryonic Stem Cells - metabolism ; Humans ; Hybrid Cells - metabolism ; Hybrid Cells - pathology ; Mice ; Original Paper ; Original Papers ; Ovarian Neoplasms - metabolism ; Ovarian Neoplasms - pathology ; PTEN Phosphohydrolase - genetics ; Xenograft Model Antitumor Assays</subject><ispartof>Bioscience reports, 2016-10, Vol.36 (5), p.e00378-e00378</ispartof><rights>2016 The Author(s).</rights><rights>2016 The Author(s) 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-c0d46fada256dee8527ed9afa1eea5cc9b4f408108468042edd9fe987fcc31663</citedby><cites>FETCH-LOGICAL-c414t-c0d46fada256dee8527ed9afa1eea5cc9b4f408108468042edd9fe987fcc31663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025808/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5025808/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27377320$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Ke</creatorcontrib><creatorcontrib>Qu, Hu</creatorcontrib><creatorcontrib>Xu, Li-Nan</creatorcontrib><creatorcontrib>Gao, Jun</creatorcontrib><creatorcontrib>Cheng, Fu-Yi</creatorcontrib><creatorcontrib>Xiang, Peng</creatorcontrib><creatorcontrib>Zhou, Can-Quan</creatorcontrib><title>Epigenetics changes caused by the fusion of human embryonic stem cell and ovarian cancer cells</title><title>Bioscience reports</title><addtitle>Biosci Rep</addtitle><description>To observe the effect of gene expression and tumorigenicity in hybrid cells of human embryonic stem cells (hESCs) and ovarian cancer cells in vitro and in vivo using a mouse model, and to determine its feasibility in reprogramming tumour cells growth and apoptosis, for a potential exploration of the role of hESCs and tumour cells fusion in the management of ovarian cancer. Stable transgenic hESCs (H1) and ovarian cancer cell line OVCAR-3 were established before fusion, and cell fusion system was established to analyse the related indicators. PTEN expression in HO-H1 cells was higher than those in the parental stem cells and lower than those in parental tumour cells; the growth of OV-H1 (RFP+GFP) hybrid cells with double fluorescence expressions were obviously slower than that of human embryonic stem cells and OVCAR-3 ovarian cancer cells. The apoptosis signal of the OV-H1 hybrid cells was significantly higher than that of the hESCs and OVCAR-3 ovarian cancer cells. In vivo results showed that compared with 7 days, 28 days and 35 days after inoculation of OV-H1 hybrid cells; also, apoptotic cell detection indicated that much stronger apoptotic signal was found in OV-H1 hybrid cells inoculated mouse. The hESCs can inhibit the growth of OVCAR-3 cells in vitro by suppressing p53 and PTEN expression to suppress the growth of tumour that may be achieved by inducing apoptosis of OVCAR-3 cells. The change of epigenetics after fusion of ovarian cancer cells and hESCs may become a novel direction for treatment of ovarian cancer.</description><subject>Animals</subject><subject>Apoptosis - genetics</subject><subject>Cell Fusion</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - genetics</subject><subject>Drug Resistance, Neoplasm</subject><subject>Epigenesis, Genetic</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Human Embryonic Stem Cells - cytology</subject><subject>Human Embryonic Stem Cells - metabolism</subject><subject>Humans</subject><subject>Hybrid Cells - metabolism</subject><subject>Hybrid Cells - pathology</subject><subject>Mice</subject><subject>Original Paper</subject><subject>Original Papers</subject><subject>Ovarian Neoplasms - metabolism</subject><subject>Ovarian Neoplasms - pathology</subject><subject>PTEN Phosphohydrolase - genetics</subject><subject>Xenograft Model Antitumor Assays</subject><issn>0144-8463</issn><issn>1573-4935</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFLHDEUxkNR6qo99V5yFMrUl0lmJnMpqGhbEARbr4Zs8rKbspOsyYyw_32juxW9eXq89_34eB8fIZ8ZfGMg6tPz37c1sBbK8oHMWNPxSvS82SMzYEJUUrT8gBzm_BcAiiA-koO6413Ha5iR-8u1X2DA0ZtMzVKHBZapp4yWzjd0XCJ1U_Yx0Ojochp0oDjM0yYGb2gecaAGVyuqg6XxUSdfdKODwfR8z8dk3-lVxk-7eUTuri7_XPysrm9-_Lo4u66MYGKsDFjROm113bQWUTZ1h7bXTjNE3RjTz4UTIBmUMLJkRmt7h73snDGctS0_It-3vutpPqA1GMakV2qd_KDTRkXt1Vsl-KVaxEfVQN1IkMXgZGeQ4sOEeVSDz08RdMA4ZcVkKznjwNt3oDU0vRBcFPTrFjUp5pzQvXzEQD2Vp16VV-gvr0O8sP_b4v8AS-2WQA</recordid><startdate>20161001</startdate><enddate>20161001</enddate><creator>He, Ke</creator><creator>Qu, Hu</creator><creator>Xu, Li-Nan</creator><creator>Gao, Jun</creator><creator>Cheng, Fu-Yi</creator><creator>Xiang, Peng</creator><creator>Zhou, Can-Quan</creator><general>Portland Press Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20161001</creationdate><title>Epigenetics changes caused by the fusion of human embryonic stem cell and ovarian cancer cells</title><author>He, Ke ; Qu, Hu ; Xu, Li-Nan ; Gao, Jun ; Cheng, Fu-Yi ; Xiang, Peng ; Zhou, Can-Quan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-c0d46fada256dee8527ed9afa1eea5cc9b4f408108468042edd9fe987fcc31663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Apoptosis - genetics</topic><topic>Cell Fusion</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - genetics</topic><topic>Drug Resistance, Neoplasm</topic><topic>Epigenesis, Genetic</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Human Embryonic Stem Cells - cytology</topic><topic>Human Embryonic Stem Cells - metabolism</topic><topic>Humans</topic><topic>Hybrid Cells - metabolism</topic><topic>Hybrid Cells - pathology</topic><topic>Mice</topic><topic>Original Paper</topic><topic>Original Papers</topic><topic>Ovarian Neoplasms - metabolism</topic><topic>Ovarian Neoplasms - pathology</topic><topic>PTEN Phosphohydrolase - genetics</topic><topic>Xenograft Model Antitumor Assays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Ke</creatorcontrib><creatorcontrib>Qu, Hu</creatorcontrib><creatorcontrib>Xu, Li-Nan</creatorcontrib><creatorcontrib>Gao, Jun</creatorcontrib><creatorcontrib>Cheng, Fu-Yi</creatorcontrib><creatorcontrib>Xiang, Peng</creatorcontrib><creatorcontrib>Zhou, Can-Quan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Bioscience reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Ke</au><au>Qu, Hu</au><au>Xu, Li-Nan</au><au>Gao, Jun</au><au>Cheng, Fu-Yi</au><au>Xiang, Peng</au><au>Zhou, Can-Quan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Epigenetics changes caused by the fusion of human embryonic stem cell and ovarian cancer cells</atitle><jtitle>Bioscience reports</jtitle><addtitle>Biosci Rep</addtitle><date>2016-10-01</date><risdate>2016</risdate><volume>36</volume><issue>5</issue><spage>e00378</spage><epage>e00378</epage><pages>e00378-e00378</pages><issn>0144-8463</issn><eissn>1573-4935</eissn><abstract>To observe the effect of gene expression and tumorigenicity in hybrid cells of human embryonic stem cells (hESCs) and ovarian cancer cells in vitro and in vivo using a mouse model, and to determine its feasibility in reprogramming tumour cells growth and apoptosis, for a potential exploration of the role of hESCs and tumour cells fusion in the management of ovarian cancer. Stable transgenic hESCs (H1) and ovarian cancer cell line OVCAR-3 were established before fusion, and cell fusion system was established to analyse the related indicators. PTEN expression in HO-H1 cells was higher than those in the parental stem cells and lower than those in parental tumour cells; the growth of OV-H1 (RFP+GFP) hybrid cells with double fluorescence expressions were obviously slower than that of human embryonic stem cells and OVCAR-3 ovarian cancer cells. The apoptosis signal of the OV-H1 hybrid cells was significantly higher than that of the hESCs and OVCAR-3 ovarian cancer cells. In vivo results showed that compared with 7 days, 28 days and 35 days after inoculation of OV-H1 hybrid cells; also, apoptotic cell detection indicated that much stronger apoptotic signal was found in OV-H1 hybrid cells inoculated mouse. The hESCs can inhibit the growth of OVCAR-3 cells in vitro by suppressing p53 and PTEN expression to suppress the growth of tumour that may be achieved by inducing apoptosis of OVCAR-3 cells. The change of epigenetics after fusion of ovarian cancer cells and hESCs may become a novel direction for treatment of ovarian cancer.</abstract><cop>England</cop><pub>Portland Press Ltd</pub><pmid>27377320</pmid><doi>10.1042/BSR20160104</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Apoptosis - genetics Cell Fusion Cell Line, Tumor Cell Proliferation - genetics Drug Resistance, Neoplasm Epigenesis, Genetic Female Gene Expression Regulation, Neoplastic Human Embryonic Stem Cells - cytology Human Embryonic Stem Cells - metabolism Humans Hybrid Cells - metabolism Hybrid Cells - pathology Mice Original Paper Original Papers Ovarian Neoplasms - metabolism Ovarian Neoplasms - pathology PTEN Phosphohydrolase - genetics Xenograft Model Antitumor Assays |
| title | Epigenetics changes caused by the fusion of human embryonic stem cell and ovarian cancer cells |
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