Comparative genomics of biotechnologically important yeasts
Ascomycete yeasts are metabolically diverse, with great potential for biotechnology. Here, we report the comparative genome analysis of 29 taxonomically and biotechnologically important yeasts, including 16 newly sequenced. We identify a genetic code change, CUG-Ala, in Pachysolen tannophilus in the...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2016-08, Vol.113 (35), p.9882-9887 |
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| creator | Riley, Robert Haridas, Sajeet Wolfe, Kenneth H. Lopes, Mariana R. Hittinger, Chris Todd Göker, Markus Salamov, Asaf A. Wisecaver, Jennifer H. Long, Tanya M. Calvey, Christopher H. Aerts, Andrea L. Barry, Kerrie W. Choi, Cindy Clum, Alicia Coughlan, Aisling Y. Deshpande, Shweta Douglass, Alexander P. Hanson, Sara J. Klenk, Hans-Peter LaButti, Kurt M. Lapidus, Alla Lindquist, Erika A. Lipzen, Anna M. Meier-Kolthoff, Jan P. Ohm, Robin A. Otillar, Robert P. Pangilinan, Jasmyn L. Peng, Yi Rokas, Antonis Rosa, Carlos A. Scheuner, Carmen Sibirny, Andriy A. Slot, Jason C. Stielow, J. Benjamin Sun, Hui Kurtzman, Cletus P. Blackwell, Meredith Grigoriev, Igor V. Jeffries, Thomas W. |
| description | Ascomycete yeasts are metabolically diverse, with great potential for biotechnology. Here, we report the comparative genome analysis of 29 taxonomically and biotechnologically important yeasts, including 16 newly sequenced. We identify a genetic code change, CUG-Ala, in Pachysolen tannophilus in the clade sister to the known CUG-Ser clade. Our well-resolved yeast phylogeny shows that some traits, such as methylotrophy, are restricted to single clades, whereas others, such as L-rhamnose utilization, have patchy phylogenetic distributions. Gene clusters, with variable organization and distribution, encode many pathways of interest. Genomics can predict some biochemical traits precisely, but the genomic basis of others, such as xylose utilization, remains unresolved. Our data also provide insight into early evolution of ascomycetes. We document the loss of H3K9me2/3 heterochromatin, the origin of ascomycete mating-type switching, and panascomycete synteny at the MAT locus. These data and analyses will facilitate the engineering of efficient biosynthetic and degradative pathways and gateways for genomic manipulation. |
| doi_str_mv | 10.1073/pnas.1603941113 |
| format | Article |
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Benjamin ; Sun, Hui ; Kurtzman, Cletus P. ; Blackwell, Meredith ; Grigoriev, Igor V. ; Jeffries, Thomas W.</creator><creatorcontrib>Riley, Robert ; Haridas, Sajeet ; Wolfe, Kenneth H. ; Lopes, Mariana R. ; Hittinger, Chris Todd ; Göker, Markus ; Salamov, Asaf A. ; Wisecaver, Jennifer H. ; Long, Tanya M. ; Calvey, Christopher H. ; Aerts, Andrea L. ; Barry, Kerrie W. ; Choi, Cindy ; Clum, Alicia ; Coughlan, Aisling Y. ; Deshpande, Shweta ; Douglass, Alexander P. ; Hanson, Sara J. ; Klenk, Hans-Peter ; LaButti, Kurt M. ; Lapidus, Alla ; Lindquist, Erika A. ; Lipzen, Anna M. ; Meier-Kolthoff, Jan P. ; Ohm, Robin A. ; Otillar, Robert P. ; Pangilinan, Jasmyn L. ; Peng, Yi ; Rokas, Antonis ; Rosa, Carlos A. ; Scheuner, Carmen ; Sibirny, Andriy A. ; Slot, Jason C. ; Stielow, J. Benjamin ; Sun, Hui ; Kurtzman, Cletus P. ; Blackwell, Meredith ; Grigoriev, Igor V. ; Jeffries, Thomas W. ; Univ. of Wisconsin, Madison, WI (United States)</creatorcontrib><description>Ascomycete yeasts are metabolically diverse, with great potential for biotechnology. Here, we report the comparative genome analysis of 29 taxonomically and biotechnologically important yeasts, including 16 newly sequenced. We identify a genetic code change, CUG-Ala, in Pachysolen tannophilus in the clade sister to the known CUG-Ser clade. Our well-resolved yeast phylogeny shows that some traits, such as methylotrophy, are restricted to single clades, whereas others, such as L-rhamnose utilization, have patchy phylogenetic distributions. Gene clusters, with variable organization and distribution, encode many pathways of interest. Genomics can predict some biochemical traits precisely, but the genomic basis of others, such as xylose utilization, remains unresolved. Our data also provide insight into early evolution of ascomycetes. We document the loss of H3K9me2/3 heterochromatin, the origin of ascomycete mating-type switching, and panascomycete synteny at the MAT locus. These data and analyses will facilitate the engineering of efficient biosynthetic and degradative pathways and gateways for genomic manipulation.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.1603941113</identifier><identifier>PMID: 27535936</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Ascomycetes ; Ascomycota - classification ; Ascomycota - genetics ; Ascomycota - metabolism ; BASIC BIOLOGICAL SCIENCES ; bioenergy ; bioengineering ; Biological Sciences ; biotechnological yeasts ; Biotechnology ; Biotechnology - methods ; Evolution, Molecular ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; genetic code ; Genetic Code - genetics ; Genome, Fungal - genetics ; Genomics ; Genomics - methods ; Heterochromatin ; loci ; Metabolic Networks and Pathways - genetics ; microbiology ; multigene family ; Pachysolen tannophilus ; Phylogenetics ; Phylogeny ; rhamnose ; sequence analysis ; Species Specificity ; xylose ; Yeast ; yeasts ; Yeasts - classification ; Yeasts - genetics ; Yeasts - metabolism</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2016-08, Vol.113 (35), p.9882-9887</ispartof><rights>Volumes 1–89 and 106–113, copyright as a collective work only; author(s) retains copyright to individual articles</rights><rights>Copyright National Academy of Sciences Aug 30, 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c648t-8166e28014a503a9814a06f911c84a6dce3fbbdb4a5d543f3ccade83eada62803</citedby><cites>FETCH-LOGICAL-c648t-8166e28014a503a9814a06f911c84a6dce3fbbdb4a5d543f3ccade83eada62803</cites><orcidid>0000-0002-7248-6551 ; 0000-0001-7408-4065 ; 0000-0002-7330-4983 ; 0000000174084065 ; 0000000272486551 ; 0000000273304983</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/26471720$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/26471720$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27535936$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/1298331$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Riley, Robert</creatorcontrib><creatorcontrib>Haridas, Sajeet</creatorcontrib><creatorcontrib>Wolfe, Kenneth H.</creatorcontrib><creatorcontrib>Lopes, Mariana R.</creatorcontrib><creatorcontrib>Hittinger, Chris Todd</creatorcontrib><creatorcontrib>Göker, Markus</creatorcontrib><creatorcontrib>Salamov, Asaf A.</creatorcontrib><creatorcontrib>Wisecaver, Jennifer H.</creatorcontrib><creatorcontrib>Long, Tanya M.</creatorcontrib><creatorcontrib>Calvey, Christopher H.</creatorcontrib><creatorcontrib>Aerts, Andrea L.</creatorcontrib><creatorcontrib>Barry, Kerrie W.</creatorcontrib><creatorcontrib>Choi, Cindy</creatorcontrib><creatorcontrib>Clum, Alicia</creatorcontrib><creatorcontrib>Coughlan, Aisling Y.</creatorcontrib><creatorcontrib>Deshpande, Shweta</creatorcontrib><creatorcontrib>Douglass, Alexander P.</creatorcontrib><creatorcontrib>Hanson, Sara J.</creatorcontrib><creatorcontrib>Klenk, Hans-Peter</creatorcontrib><creatorcontrib>LaButti, Kurt M.</creatorcontrib><creatorcontrib>Lapidus, Alla</creatorcontrib><creatorcontrib>Lindquist, Erika A.</creatorcontrib><creatorcontrib>Lipzen, Anna M.</creatorcontrib><creatorcontrib>Meier-Kolthoff, Jan P.</creatorcontrib><creatorcontrib>Ohm, Robin A.</creatorcontrib><creatorcontrib>Otillar, Robert P.</creatorcontrib><creatorcontrib>Pangilinan, Jasmyn L.</creatorcontrib><creatorcontrib>Peng, Yi</creatorcontrib><creatorcontrib>Rokas, Antonis</creatorcontrib><creatorcontrib>Rosa, Carlos A.</creatorcontrib><creatorcontrib>Scheuner, Carmen</creatorcontrib><creatorcontrib>Sibirny, Andriy A.</creatorcontrib><creatorcontrib>Slot, Jason C.</creatorcontrib><creatorcontrib>Stielow, J. Benjamin</creatorcontrib><creatorcontrib>Sun, Hui</creatorcontrib><creatorcontrib>Kurtzman, Cletus P.</creatorcontrib><creatorcontrib>Blackwell, Meredith</creatorcontrib><creatorcontrib>Grigoriev, Igor V.</creatorcontrib><creatorcontrib>Jeffries, Thomas W.</creatorcontrib><creatorcontrib>Univ. of Wisconsin, Madison, WI (United States)</creatorcontrib><title>Comparative genomics of biotechnologically important yeasts</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Ascomycete yeasts are metabolically diverse, with great potential for biotechnology. Here, we report the comparative genome analysis of 29 taxonomically and biotechnologically important yeasts, including 16 newly sequenced. We identify a genetic code change, CUG-Ala, in Pachysolen tannophilus in the clade sister to the known CUG-Ser clade. Our well-resolved yeast phylogeny shows that some traits, such as methylotrophy, are restricted to single clades, whereas others, such as L-rhamnose utilization, have patchy phylogenetic distributions. Gene clusters, with variable organization and distribution, encode many pathways of interest. Genomics can predict some biochemical traits precisely, but the genomic basis of others, such as xylose utilization, remains unresolved. Our data also provide insight into early evolution of ascomycetes. We document the loss of H3K9me2/3 heterochromatin, the origin of ascomycete mating-type switching, and panascomycete synteny at the MAT locus. These data and analyses will facilitate the engineering of efficient biosynthetic and degradative pathways and gateways for genomic manipulation.</description><subject>Ascomycetes</subject><subject>Ascomycota - classification</subject><subject>Ascomycota - genetics</subject><subject>Ascomycota - metabolism</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>bioenergy</subject><subject>bioengineering</subject><subject>Biological Sciences</subject><subject>biotechnological yeasts</subject><subject>Biotechnology</subject><subject>Biotechnology - methods</subject><subject>Evolution, Molecular</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>genetic code</subject><subject>Genetic Code - genetics</subject><subject>Genome, Fungal - genetics</subject><subject>Genomics</subject><subject>Genomics - methods</subject><subject>Heterochromatin</subject><subject>loci</subject><subject>Metabolic Networks and Pathways - genetics</subject><subject>microbiology</subject><subject>multigene family</subject><subject>Pachysolen tannophilus</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>rhamnose</subject><subject>sequence analysis</subject><subject>Species Specificity</subject><subject>xylose</subject><subject>Yeast</subject><subject>yeasts</subject><subject>Yeasts - classification</subject><subject>Yeasts - genetics</subject><subject>Yeasts - metabolism</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks-L1DAYhoMo7rh69qQUvXjpbr58aZogCDL4Cxa86DmkaTqboU1qk1mY_96UWXfVi54S-J7vDXl4CXkO9AJoi5dzMOkCBEXFAQAfkA1QBbXgij4kG0pZW0vO-Bl5ktKeUqoaSR-TM9Y22CgUG_J2G6fZLCb7G1ftXIiTt6mKQ9X5mJ29DnGMO2_NOB4rP81xySbk6uhMyukpeTSYMblnt-c5-f7xw7ft5_rq66cv2_dXtRVc5lqCEI5JCtw0FI2S5ULFoACs5Eb01uHQdX1Xxn3DcUBrTe8kOtMbUfbwnLw75c6HbnKFD3kxo54XP5nlqKPx-s9J8Nd6F290QxkXKEvAq1NATNnrZP36MxtDcDZrYEoiQoHe3L6yxB8Hl7KefLJuHE1w8ZA0K_qKaGjxnyhI1ioEkPw_UGhUKwSqgr7-C93HwxKK2ZUquliDa-DlibJLTGlxw50HoHrthF47oe87UTZe_q7vjv9VggK8OAH7lONyPxe8hZZR_AmT9bun</recordid><startdate>20160830</startdate><enddate>20160830</enddate><creator>Riley, Robert</creator><creator>Haridas, Sajeet</creator><creator>Wolfe, Kenneth H.</creator><creator>Lopes, Mariana R.</creator><creator>Hittinger, Chris Todd</creator><creator>Göker, Markus</creator><creator>Salamov, Asaf A.</creator><creator>Wisecaver, Jennifer H.</creator><creator>Long, Tanya M.</creator><creator>Calvey, Christopher H.</creator><creator>Aerts, Andrea L.</creator><creator>Barry, Kerrie W.</creator><creator>Choi, Cindy</creator><creator>Clum, Alicia</creator><creator>Coughlan, Aisling Y.</creator><creator>Deshpande, Shweta</creator><creator>Douglass, Alexander P.</creator><creator>Hanson, Sara J.</creator><creator>Klenk, Hans-Peter</creator><creator>LaButti, Kurt M.</creator><creator>Lapidus, Alla</creator><creator>Lindquist, Erika A.</creator><creator>Lipzen, Anna M.</creator><creator>Meier-Kolthoff, Jan P.</creator><creator>Ohm, Robin A.</creator><creator>Otillar, Robert P.</creator><creator>Pangilinan, Jasmyn L.</creator><creator>Peng, Yi</creator><creator>Rokas, Antonis</creator><creator>Rosa, Carlos A.</creator><creator>Scheuner, Carmen</creator><creator>Sibirny, Andriy A.</creator><creator>Slot, Jason C.</creator><creator>Stielow, J. 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Benjamin ; Sun, Hui ; Kurtzman, Cletus P. ; Blackwell, Meredith ; Grigoriev, Igor V. ; Jeffries, Thomas W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c648t-8166e28014a503a9814a06f911c84a6dce3fbbdb4a5d543f3ccade83eada62803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Ascomycetes</topic><topic>Ascomycota - classification</topic><topic>Ascomycota - genetics</topic><topic>Ascomycota - metabolism</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>bioenergy</topic><topic>bioengineering</topic><topic>Biological Sciences</topic><topic>biotechnological yeasts</topic><topic>Biotechnology</topic><topic>Biotechnology - methods</topic><topic>Evolution, Molecular</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>genetic code</topic><topic>Genetic Code - genetics</topic><topic>Genome, Fungal - genetics</topic><topic>Genomics</topic><topic>Genomics - methods</topic><topic>Heterochromatin</topic><topic>loci</topic><topic>Metabolic Networks and Pathways - genetics</topic><topic>microbiology</topic><topic>multigene family</topic><topic>Pachysolen tannophilus</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>rhamnose</topic><topic>sequence analysis</topic><topic>Species Specificity</topic><topic>xylose</topic><topic>Yeast</topic><topic>yeasts</topic><topic>Yeasts - classification</topic><topic>Yeasts - genetics</topic><topic>Yeasts - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Riley, Robert</creatorcontrib><creatorcontrib>Haridas, Sajeet</creatorcontrib><creatorcontrib>Wolfe, Kenneth H.</creatorcontrib><creatorcontrib>Lopes, Mariana R.</creatorcontrib><creatorcontrib>Hittinger, Chris Todd</creatorcontrib><creatorcontrib>Göker, Markus</creatorcontrib><creatorcontrib>Salamov, Asaf A.</creatorcontrib><creatorcontrib>Wisecaver, Jennifer H.</creatorcontrib><creatorcontrib>Long, Tanya M.</creatorcontrib><creatorcontrib>Calvey, Christopher H.</creatorcontrib><creatorcontrib>Aerts, Andrea L.</creatorcontrib><creatorcontrib>Barry, Kerrie W.</creatorcontrib><creatorcontrib>Choi, Cindy</creatorcontrib><creatorcontrib>Clum, Alicia</creatorcontrib><creatorcontrib>Coughlan, Aisling Y.</creatorcontrib><creatorcontrib>Deshpande, Shweta</creatorcontrib><creatorcontrib>Douglass, Alexander P.</creatorcontrib><creatorcontrib>Hanson, Sara J.</creatorcontrib><creatorcontrib>Klenk, Hans-Peter</creatorcontrib><creatorcontrib>LaButti, Kurt M.</creatorcontrib><creatorcontrib>Lapidus, Alla</creatorcontrib><creatorcontrib>Lindquist, Erika A.</creatorcontrib><creatorcontrib>Lipzen, Anna M.</creatorcontrib><creatorcontrib>Meier-Kolthoff, Jan P.</creatorcontrib><creatorcontrib>Ohm, Robin A.</creatorcontrib><creatorcontrib>Otillar, Robert P.</creatorcontrib><creatorcontrib>Pangilinan, Jasmyn L.</creatorcontrib><creatorcontrib>Peng, Yi</creatorcontrib><creatorcontrib>Rokas, Antonis</creatorcontrib><creatorcontrib>Rosa, Carlos A.</creatorcontrib><creatorcontrib>Scheuner, Carmen</creatorcontrib><creatorcontrib>Sibirny, Andriy A.</creatorcontrib><creatorcontrib>Slot, Jason C.</creatorcontrib><creatorcontrib>Stielow, J. Benjamin</creatorcontrib><creatorcontrib>Sun, Hui</creatorcontrib><creatorcontrib>Kurtzman, Cletus P.</creatorcontrib><creatorcontrib>Blackwell, Meredith</creatorcontrib><creatorcontrib>Grigoriev, Igor V.</creatorcontrib><creatorcontrib>Jeffries, Thomas W.</creatorcontrib><creatorcontrib>Univ. of Wisconsin, Madison, WI (United States)</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Riley, Robert</au><au>Haridas, Sajeet</au><au>Wolfe, Kenneth H.</au><au>Lopes, Mariana R.</au><au>Hittinger, Chris Todd</au><au>Göker, Markus</au><au>Salamov, Asaf A.</au><au>Wisecaver, Jennifer H.</au><au>Long, Tanya M.</au><au>Calvey, Christopher H.</au><au>Aerts, Andrea L.</au><au>Barry, Kerrie W.</au><au>Choi, Cindy</au><au>Clum, Alicia</au><au>Coughlan, Aisling Y.</au><au>Deshpande, Shweta</au><au>Douglass, Alexander P.</au><au>Hanson, Sara J.</au><au>Klenk, Hans-Peter</au><au>LaButti, Kurt M.</au><au>Lapidus, Alla</au><au>Lindquist, Erika A.</au><au>Lipzen, Anna M.</au><au>Meier-Kolthoff, Jan P.</au><au>Ohm, Robin A.</au><au>Otillar, Robert P.</au><au>Pangilinan, Jasmyn L.</au><au>Peng, Yi</au><au>Rokas, Antonis</au><au>Rosa, Carlos A.</au><au>Scheuner, Carmen</au><au>Sibirny, Andriy A.</au><au>Slot, Jason C.</au><au>Stielow, J. Benjamin</au><au>Sun, Hui</au><au>Kurtzman, Cletus P.</au><au>Blackwell, Meredith</au><au>Grigoriev, Igor V.</au><au>Jeffries, Thomas W.</au><aucorp>Univ. of Wisconsin, Madison, WI (United States)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative genomics of biotechnologically important yeasts</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>2016-08-30</date><risdate>2016</risdate><volume>113</volume><issue>35</issue><spage>9882</spage><epage>9887</epage><pages>9882-9887</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Ascomycete yeasts are metabolically diverse, with great potential for biotechnology. Here, we report the comparative genome analysis of 29 taxonomically and biotechnologically important yeasts, including 16 newly sequenced. We identify a genetic code change, CUG-Ala, in Pachysolen tannophilus in the clade sister to the known CUG-Ser clade. Our well-resolved yeast phylogeny shows that some traits, such as methylotrophy, are restricted to single clades, whereas others, such as L-rhamnose utilization, have patchy phylogenetic distributions. Gene clusters, with variable organization and distribution, encode many pathways of interest. Genomics can predict some biochemical traits precisely, but the genomic basis of others, such as xylose utilization, remains unresolved. Our data also provide insight into early evolution of ascomycetes. We document the loss of H3K9me2/3 heterochromatin, the origin of ascomycete mating-type switching, and panascomycete synteny at the MAT locus. These data and analyses will facilitate the engineering of efficient biosynthetic and degradative pathways and gateways for genomic manipulation.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>27535936</pmid><doi>10.1073/pnas.1603941113</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-7248-6551</orcidid><orcidid>https://orcid.org/0000-0001-7408-4065</orcidid><orcidid>https://orcid.org/0000-0002-7330-4983</orcidid><orcidid>https://orcid.org/0000000174084065</orcidid><orcidid>https://orcid.org/0000000272486551</orcidid><orcidid>https://orcid.org/0000000273304983</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 2016-08, Vol.113 (35), p.9882-9887 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5024638 |
| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Ascomycetes Ascomycota - classification Ascomycota - genetics Ascomycota - metabolism BASIC BIOLOGICAL SCIENCES bioenergy bioengineering Biological Sciences biotechnological yeasts Biotechnology Biotechnology - methods Evolution, Molecular Fungal Proteins - genetics Fungal Proteins - metabolism genetic code Genetic Code - genetics Genome, Fungal - genetics Genomics Genomics - methods Heterochromatin loci Metabolic Networks and Pathways - genetics microbiology multigene family Pachysolen tannophilus Phylogenetics Phylogeny rhamnose sequence analysis Species Specificity xylose Yeast yeasts Yeasts - classification Yeasts - genetics Yeasts - metabolism |
| title | Comparative genomics of biotechnologically important yeasts |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T15%3A04%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20genomics%20of%20biotechnologically%20important%20yeasts&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Riley,%20Robert&rft.aucorp=Univ.%20of%20Wisconsin,%20Madison,%20WI%20(United%20States)&rft.date=2016-08-30&rft.volume=113&rft.issue=35&rft.spage=9882&rft.epage=9887&rft.pages=9882-9887&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.1603941113&rft_dat=%3Cjstor_pubme%3E26471720%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1819112534&rft_id=info:pmid/27535936&rft_jstor_id=26471720&rfr_iscdi=true |