Differential developmental competence and gene expression patterns in buffalo (Bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells
The developmental ability and gene expression pattern at 8- to 16-cell and blastocyst stages of buffalo ( Bubalus bubalis ) nuclear transfer (NT) embryos from fetal fibroblasts (FFs), amnion mesenchymal stem cells (AMSCs) and in vitro fertilized (IVF) embryos were compared in the present studies. Th...
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| creator | Sadeesh EM Shah, Fozia Yadav, P. S. |
| description | The developmental ability and gene expression pattern at 8- to 16-cell and blastocyst stages of buffalo (
Bubalus bubalis
) nuclear transfer (NT) embryos from fetal fibroblasts (FFs), amnion mesenchymal stem cells (AMSCs) and in vitro fertilized (IVF) embryos were compared in the present studies. The in vitro expanded buffalo FFs showed a typical “S” shape growth curve with a doubling time of 41.4 h and stained positive for vimentin. The in vitro cultured undifferentiated AMSCs showed a doubling time of 39.5 h and stained positive for alkaline phosphatase, and these cells also showed expression of pluripotency markers (
OCT
4
,
SOX
2
,
NANOG
), and mesenchymal stem cell markers (
CD29
,
CD44
) and were negative for haematopoietic marker (
CD34
) genes at different passages. Further, when AMSCs were exposed to corresponding induction conditions, these cells differentiated into adipogenic, chondrogenic and osteogenic lineages which were confirmed through oil red O, alcian blue and alizarin staining, respectively. Donor cells at 3–4 passage were employed for NT. The cleavage rate was significantly (
P
|
| doi_str_mv | 10.1007/s10616-015-9936-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5023557</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1820601095</sourcerecordid><originalsourceid>FETCH-LOGICAL-c535t-4adf3d475229135febe1e4742e245bed4c76a024a4d5dbe8a50822f0439cd58e3</originalsourceid><addsrcrecordid>eNp9Uk1v1DAQtRCILgs_gAuyxKUcArZj5-OCBOVTqsQFzpbjTHZdJXbwOIXtn-Ov4bClKkjgy2g8b96bsR8hjzl7zhmrXyBnFa8KxlXRtmVVXN0hG67qsmB13dwlG9YKVrSsak_IA8QLxlhb8_I-ORFVVTFZVxvy440bBojgkzMj7eESxjBPOc2ZDdMMCbwFanxPd-CBwvc5AqILns4mJYgeqfO0W4bBjIGevl46My6YL3J0-Iz6xY5gIk3ReMxKFKYuHgLSCDZ4THGxCXr6zaU9HWCVHVwXQzcaTPhL10x-lZsA8yj7w5QhmGCiFsYRH5J7WRjh0XXcki_v3n4--1Ccf3r_8ezVeWFVqVIhTT-UvayVEC0v1QAdcJC1FCCk6qCXtq4ME9LIXvUdNEaxRoiBybK1vWqg3JKXR9556SbobX6haEY9RzeZeNDBOP1nxbu93oVLrZgoVf6TLTm9Jojh6wKY9ORwXcF4CAtq3ghWMc5alaFP_4JehCX6vJ7O0zeiLpv8jf9B8abh-TC5cvEjysaAGGG4GZkzvZpIH02ks4n0aiJ9lXue3N71puO3azJAHAGYS34H8Zb0P1l_ApXZ2M8</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2918273871</pqid></control><display><type>article</type><title>Differential developmental competence and gene expression patterns in buffalo (Bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Springer Nature - Complete Springer Journals</source><source>ProQuest Central UK/Ireland</source><source>PubMed Central</source><source>ProQuest Central</source><creator>Sadeesh EM ; Shah, Fozia ; Yadav, P. S.</creator><creatorcontrib>Sadeesh EM ; Shah, Fozia ; Yadav, P. S.</creatorcontrib><description>The developmental ability and gene expression pattern at 8- to 16-cell and blastocyst stages of buffalo (
Bubalus bubalis
) nuclear transfer (NT) embryos from fetal fibroblasts (FFs), amnion mesenchymal stem cells (AMSCs) and in vitro fertilized (IVF) embryos were compared in the present studies. The in vitro expanded buffalo FFs showed a typical “S” shape growth curve with a doubling time of 41.4 h and stained positive for vimentin. The in vitro cultured undifferentiated AMSCs showed a doubling time of 39.5 h and stained positive for alkaline phosphatase, and these cells also showed expression of pluripotency markers (
OCT
4
,
SOX
2
,
NANOG
), and mesenchymal stem cell markers (
CD29
,
CD44
) and were negative for haematopoietic marker (
CD34
) genes at different passages. Further, when AMSCs were exposed to corresponding induction conditions, these cells differentiated into adipogenic, chondrogenic and osteogenic lineages which were confirmed through oil red O, alcian blue and alizarin staining, respectively. Donor cells at 3–4 passage were employed for NT. The cleavage rate was significantly (
P
< 0.05) higher in IVF than in FF-NT and AMSC-NT embryos (82.6 ± 8.2 vs. 64.6 ± 1.3 and 72.3 ± 2.2 %, respectively). However, blastocyst rates in IVF and AMSC-NT embryos (30.6 ± 2.7 and 28.9 ± 3.1 %) did not differ and were significantly (
P
< 0.05) higher than FF-NT (19.5 ± 1.8 %). Total cell number did not show significant (
P
> 0.05) differences between IVF and AMSC-NT embryos (186.7 ± 4.2, 171.2 ± 3.8, respectively) but were significantly (
P
< 0.05) higher than that from FF-NT (151.3 ± 4.1). Alterations in the expression pattern of genes implicated in transcription and pluripotency (
OCT4
,
STAT3
,
NANOG
), DNA methylation (
DNMT1
,
DNMT3A
), histone deacetylation (
HDAC2
), growth factor signaling and imprinting (
IGF2
,
IGF2R
), apoptosis (
BAX
,
BCL2
), metabolism (
GLUT1
) and oxidative stress (
MnSOD
) regulation were observed in cloned embryos. The transcripts or expression patterns in AMSC-NT embryos more closely followed that of the in vitro derived embryos compared with FF-NT embryos. The results demonstrate that multipotent amnion MSCs have a greater potential as donor cells than FFs in achieving enhanced production of cloned buffalo embryos.</description><identifier>ISSN: 0920-9069</identifier><identifier>EISSN: 1573-0778</identifier><identifier>DOI: 10.1007/s10616-015-9936-z</identifier><identifier>PMID: 26660476</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Alkaline phosphatase ; Amnion ; Animals ; Apoptosis ; BAX protein ; Bcl-2 protein ; Biochemistry ; Biomedicine ; Biotechnology ; Bone marrow ; Bubalus bubalis ; CD29 antigen ; CD34 antigen ; CD44 antigen ; Cell culture ; Cell differentiation ; Cell number ; Chemistry ; Chemistry and Materials Science ; Cloning ; Deacetylation ; Developmental stages ; DNA methylation ; DNMT1 protein ; Efficiency ; Embryos ; Ethics ; Fetuses ; Fibroblasts ; Gene expression ; Genomic imprinting ; HDAC2 protein ; Hematopoietic stem cells ; Histone deacetylase ; Histones ; Insulin-like growth factor II ; Insulin-like growth factor II receptors ; Mesenchymal stem cells ; Mesenchyme ; Nuclear transfer ; Original ; Original Article ; Penicillin ; Pluripotency ; Stem cells ; Transcription</subject><ispartof>Cytotechnology (Dordrecht), 2016-10, Vol.68 (5), p.1827-1848</ispartof><rights>Springer Science+Business Media Dordrecht 2015</rights><rights>Copyright Springer Science & Business Media 2016</rights><rights>Springer Science+Business Media Dordrecht 2015.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c535t-4adf3d475229135febe1e4742e245bed4c76a024a4d5dbe8a50822f0439cd58e3</citedby><cites>FETCH-LOGICAL-c535t-4adf3d475229135febe1e4742e245bed4c76a024a4d5dbe8a50822f0439cd58e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5023557/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2918273871?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,314,725,778,782,883,21375,27911,27912,33731,33732,41475,42544,43792,51306,53778,53780,64370,64372,64374,72226</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26660476$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sadeesh EM</creatorcontrib><creatorcontrib>Shah, Fozia</creatorcontrib><creatorcontrib>Yadav, P. S.</creatorcontrib><title>Differential developmental competence and gene expression patterns in buffalo (Bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells</title><title>Cytotechnology (Dordrecht)</title><addtitle>Cytotechnology</addtitle><addtitle>Cytotechnology</addtitle><description>The developmental ability and gene expression pattern at 8- to 16-cell and blastocyst stages of buffalo (
Bubalus bubalis
) nuclear transfer (NT) embryos from fetal fibroblasts (FFs), amnion mesenchymal stem cells (AMSCs) and in vitro fertilized (IVF) embryos were compared in the present studies. The in vitro expanded buffalo FFs showed a typical “S” shape growth curve with a doubling time of 41.4 h and stained positive for vimentin. The in vitro cultured undifferentiated AMSCs showed a doubling time of 39.5 h and stained positive for alkaline phosphatase, and these cells also showed expression of pluripotency markers (
OCT
4
,
SOX
2
,
NANOG
), and mesenchymal stem cell markers (
CD29
,
CD44
) and were negative for haematopoietic marker (
CD34
) genes at different passages. Further, when AMSCs were exposed to corresponding induction conditions, these cells differentiated into adipogenic, chondrogenic and osteogenic lineages which were confirmed through oil red O, alcian blue and alizarin staining, respectively. Donor cells at 3–4 passage were employed for NT. The cleavage rate was significantly (
P
< 0.05) higher in IVF than in FF-NT and AMSC-NT embryos (82.6 ± 8.2 vs. 64.6 ± 1.3 and 72.3 ± 2.2 %, respectively). However, blastocyst rates in IVF and AMSC-NT embryos (30.6 ± 2.7 and 28.9 ± 3.1 %) did not differ and were significantly (
P
< 0.05) higher than FF-NT (19.5 ± 1.8 %). Total cell number did not show significant (
P
> 0.05) differences between IVF and AMSC-NT embryos (186.7 ± 4.2, 171.2 ± 3.8, respectively) but were significantly (
P
< 0.05) higher than that from FF-NT (151.3 ± 4.1). Alterations in the expression pattern of genes implicated in transcription and pluripotency (
OCT4
,
STAT3
,
NANOG
), DNA methylation (
DNMT1
,
DNMT3A
), histone deacetylation (
HDAC2
), growth factor signaling and imprinting (
IGF2
,
IGF2R
), apoptosis (
BAX
,
BCL2
), metabolism (
GLUT1
) and oxidative stress (
MnSOD
) regulation were observed in cloned embryos. The transcripts or expression patterns in AMSC-NT embryos more closely followed that of the in vitro derived embryos compared with FF-NT embryos. The results demonstrate that multipotent amnion MSCs have a greater potential as donor cells than FFs in achieving enhanced production of cloned buffalo embryos.</description><subject>Alkaline phosphatase</subject><subject>Amnion</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>BAX protein</subject><subject>Bcl-2 protein</subject><subject>Biochemistry</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Bone marrow</subject><subject>Bubalus bubalis</subject><subject>CD29 antigen</subject><subject>CD34 antigen</subject><subject>CD44 antigen</subject><subject>Cell culture</subject><subject>Cell differentiation</subject><subject>Cell number</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cloning</subject><subject>Deacetylation</subject><subject>Developmental stages</subject><subject>DNA methylation</subject><subject>DNMT1 protein</subject><subject>Efficiency</subject><subject>Embryos</subject><subject>Ethics</subject><subject>Fetuses</subject><subject>Fibroblasts</subject><subject>Gene expression</subject><subject>Genomic imprinting</subject><subject>HDAC2 protein</subject><subject>Hematopoietic stem cells</subject><subject>Histone deacetylase</subject><subject>Histones</subject><subject>Insulin-like growth factor II</subject><subject>Insulin-like growth factor II receptors</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchyme</subject><subject>Nuclear transfer</subject><subject>Original</subject><subject>Original Article</subject><subject>Penicillin</subject><subject>Pluripotency</subject><subject>Stem cells</subject><subject>Transcription</subject><issn>0920-9069</issn><issn>1573-0778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9Uk1v1DAQtRCILgs_gAuyxKUcArZj5-OCBOVTqsQFzpbjTHZdJXbwOIXtn-Ov4bClKkjgy2g8b96bsR8hjzl7zhmrXyBnFa8KxlXRtmVVXN0hG67qsmB13dwlG9YKVrSsak_IA8QLxlhb8_I-ORFVVTFZVxvy440bBojgkzMj7eESxjBPOc2ZDdMMCbwFanxPd-CBwvc5AqILns4mJYgeqfO0W4bBjIGevl46My6YL3J0-Iz6xY5gIk3ReMxKFKYuHgLSCDZ4THGxCXr6zaU9HWCVHVwXQzcaTPhL10x-lZsA8yj7w5QhmGCiFsYRH5J7WRjh0XXcki_v3n4--1Ccf3r_8ezVeWFVqVIhTT-UvayVEC0v1QAdcJC1FCCk6qCXtq4ME9LIXvUdNEaxRoiBybK1vWqg3JKXR9556SbobX6haEY9RzeZeNDBOP1nxbu93oVLrZgoVf6TLTm9Jojh6wKY9ORwXcF4CAtq3ghWMc5alaFP_4JehCX6vJ7O0zeiLpv8jf9B8abh-TC5cvEjysaAGGG4GZkzvZpIH02ks4n0aiJ9lXue3N71puO3azJAHAGYS34H8Zb0P1l_ApXZ2M8</recordid><startdate>20161001</startdate><enddate>20161001</enddate><creator>Sadeesh EM</creator><creator>Shah, Fozia</creator><creator>Yadav, P. S.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20161001</creationdate><title>Differential developmental competence and gene expression patterns in buffalo (Bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells</title><author>Sadeesh EM ; Shah, Fozia ; Yadav, P. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c535t-4adf3d475229135febe1e4742e245bed4c76a024a4d5dbe8a50822f0439cd58e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Alkaline phosphatase</topic><topic>Amnion</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>BAX protein</topic><topic>Bcl-2 protein</topic><topic>Biochemistry</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Bone marrow</topic><topic>Bubalus bubalis</topic><topic>CD29 antigen</topic><topic>CD34 antigen</topic><topic>CD44 antigen</topic><topic>Cell culture</topic><topic>Cell differentiation</topic><topic>Cell number</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cloning</topic><topic>Deacetylation</topic><topic>Developmental stages</topic><topic>DNA methylation</topic><topic>DNMT1 protein</topic><topic>Efficiency</topic><topic>Embryos</topic><topic>Ethics</topic><topic>Fetuses</topic><topic>Fibroblasts</topic><topic>Gene expression</topic><topic>Genomic imprinting</topic><topic>HDAC2 protein</topic><topic>Hematopoietic stem cells</topic><topic>Histone deacetylase</topic><topic>Histones</topic><topic>Insulin-like growth factor II</topic><topic>Insulin-like growth factor II receptors</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchyme</topic><topic>Nuclear transfer</topic><topic>Original</topic><topic>Original Article</topic><topic>Penicillin</topic><topic>Pluripotency</topic><topic>Stem cells</topic><topic>Transcription</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sadeesh EM</creatorcontrib><creatorcontrib>Shah, Fozia</creatorcontrib><creatorcontrib>Yadav, P. S.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cytotechnology (Dordrecht)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sadeesh EM</au><au>Shah, Fozia</au><au>Yadav, P. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential developmental competence and gene expression patterns in buffalo (Bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells</atitle><jtitle>Cytotechnology (Dordrecht)</jtitle><stitle>Cytotechnology</stitle><addtitle>Cytotechnology</addtitle><date>2016-10-01</date><risdate>2016</risdate><volume>68</volume><issue>5</issue><spage>1827</spage><epage>1848</epage><pages>1827-1848</pages><issn>0920-9069</issn><eissn>1573-0778</eissn><abstract>The developmental ability and gene expression pattern at 8- to 16-cell and blastocyst stages of buffalo (
Bubalus bubalis
) nuclear transfer (NT) embryos from fetal fibroblasts (FFs), amnion mesenchymal stem cells (AMSCs) and in vitro fertilized (IVF) embryos were compared in the present studies. The in vitro expanded buffalo FFs showed a typical “S” shape growth curve with a doubling time of 41.4 h and stained positive for vimentin. The in vitro cultured undifferentiated AMSCs showed a doubling time of 39.5 h and stained positive for alkaline phosphatase, and these cells also showed expression of pluripotency markers (
OCT
4
,
SOX
2
,
NANOG
), and mesenchymal stem cell markers (
CD29
,
CD44
) and were negative for haematopoietic marker (
CD34
) genes at different passages. Further, when AMSCs were exposed to corresponding induction conditions, these cells differentiated into adipogenic, chondrogenic and osteogenic lineages which were confirmed through oil red O, alcian blue and alizarin staining, respectively. Donor cells at 3–4 passage were employed for NT. The cleavage rate was significantly (
P
< 0.05) higher in IVF than in FF-NT and AMSC-NT embryos (82.6 ± 8.2 vs. 64.6 ± 1.3 and 72.3 ± 2.2 %, respectively). However, blastocyst rates in IVF and AMSC-NT embryos (30.6 ± 2.7 and 28.9 ± 3.1 %) did not differ and were significantly (
P
< 0.05) higher than FF-NT (19.5 ± 1.8 %). Total cell number did not show significant (
P
> 0.05) differences between IVF and AMSC-NT embryos (186.7 ± 4.2, 171.2 ± 3.8, respectively) but were significantly (
P
< 0.05) higher than that from FF-NT (151.3 ± 4.1). Alterations in the expression pattern of genes implicated in transcription and pluripotency (
OCT4
,
STAT3
,
NANOG
), DNA methylation (
DNMT1
,
DNMT3A
), histone deacetylation (
HDAC2
), growth factor signaling and imprinting (
IGF2
,
IGF2R
), apoptosis (
BAX
,
BCL2
), metabolism (
GLUT1
) and oxidative stress (
MnSOD
) regulation were observed in cloned embryos. The transcripts or expression patterns in AMSC-NT embryos more closely followed that of the in vitro derived embryos compared with FF-NT embryos. The results demonstrate that multipotent amnion MSCs have a greater potential as donor cells than FFs in achieving enhanced production of cloned buffalo embryos.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>26660476</pmid><doi>10.1007/s10616-015-9936-z</doi><tpages>22</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Cytotechnology (Dordrecht), 2016-10, Vol.68 (5), p.1827-1848 |
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| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5023557 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Springer Nature - Complete Springer Journals; ProQuest Central UK/Ireland; PubMed Central; ProQuest Central |
| subjects | Alkaline phosphatase Amnion Animals Apoptosis BAX protein Bcl-2 protein Biochemistry Biomedicine Biotechnology Bone marrow Bubalus bubalis CD29 antigen CD34 antigen CD44 antigen Cell culture Cell differentiation Cell number Chemistry Chemistry and Materials Science Cloning Deacetylation Developmental stages DNA methylation DNMT1 protein Efficiency Embryos Ethics Fetuses Fibroblasts Gene expression Genomic imprinting HDAC2 protein Hematopoietic stem cells Histone deacetylase Histones Insulin-like growth factor II Insulin-like growth factor II receptors Mesenchymal stem cells Mesenchyme Nuclear transfer Original Original Article Penicillin Pluripotency Stem cells Transcription |
| title | Differential developmental competence and gene expression patterns in buffalo (Bubalus bubalis) nuclear transfer embryos reconstructed with fetal fibroblasts and amnion mesenchymal stem cells |
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