Adherence of Candida albicans to oral epithelial cells differentiated by Papanicolaou staining
OBJECTIVE: To examine the relative adherence of Candida albicans to oral epithelial cells differentiated by Papanicolaou staining. METHODS: Oral epithelial cells were collected from 10 healthy adults (five male, five female) and counted. Equal volumes of oral epithelial cells and candida were mixed...
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| Veröffentlicht in: | Journal of clinical pathology 1999-07, Vol.52 (7), p.529-531 |
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| creator | Williams, D W Walker, R Lewis, M A Allison, R T Potts, A J |
| description | OBJECTIVE: To examine the relative adherence of Candida albicans to oral epithelial cells differentiated by Papanicolaou staining. METHODS: Oral epithelial cells were collected from 10 healthy adults (five male, five female) and counted. Equal volumes of oral epithelial cells and candida were mixed and incubated. The epithelial cells from this mix were collected by filtration through 10 microns polycarbonate membrane filters. Cells retained on the membrane filters were stained with crystal violet followed by Papanicolaou stain. The number of yeast attached to each of 100 red, orange, and green staining oral epithelial cells was determined by direct microscopic examination. RESULTS: C albicans had a higher level of adherence (p < 0.001) to red staining oral epithelial cells (mean (SD) number of candida attached to 100 oral epithelial cells 562 (159)) than to cells staining either orange (105 (47)) or green (161 (66)). CONCLUSIONS: Oral epithelial cell variability for candidal adherence is confirmed. The technique provides an opportunity to examine the relation between oral epithelial cell type and oral candidosis in specific groups, such as tobacco smokers, where increased epithelial cell keratinisation and candidal colonisation has been reported. |
| doi_str_mv | 10.1136/jcp.52.7.529 |
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METHODS: Oral epithelial cells were collected from 10 healthy adults (five male, five female) and counted. Equal volumes of oral epithelial cells and candida were mixed and incubated. The epithelial cells from this mix were collected by filtration through 10 microns polycarbonate membrane filters. Cells retained on the membrane filters were stained with crystal violet followed by Papanicolaou stain. The number of yeast attached to each of 100 red, orange, and green staining oral epithelial cells was determined by direct microscopic examination. RESULTS: C albicans had a higher level of adherence (p < 0.001) to red staining oral epithelial cells (mean (SD) number of candida attached to 100 oral epithelial cells 562 (159)) than to cells staining either orange (105 (47)) or green (161 (66)). CONCLUSIONS: Oral epithelial cell variability for candidal adherence is confirmed. The technique provides an opportunity to examine the relation between oral epithelial cell type and oral candidosis in specific groups, such as tobacco smokers, where increased epithelial cell keratinisation and candidal colonisation has been reported.</description><identifier>ISSN: 0021-9746</identifier><identifier>EISSN: 1472-4146</identifier><identifier>DOI: 10.1136/jcp.52.7.529</identifier><identifier>PMID: 10605408</identifier><identifier>CODEN: JCPAAK</identifier><language>eng</language><publisher>London: BMJ Publishing Group Ltd and Association of Clinical Pathologists</publisher><subject>Adult ; Analysis of Variance ; Biological and medical sciences ; Candida albicans - physiology ; Cell Adhesion - physiology ; Cells, Cultured ; Coloring Agents ; Ent and stomatologic mycoses ; Epithelial Cells - microbiology ; Female ; Human mycoses ; Humans ; Infectious diseases ; Keratinocytes ; Male ; Medical sciences ; Mouth Mucosa - cytology ; Mouth Mucosa - microbiology ; Mycoses ; Specimen Handling - methods</subject><ispartof>Journal of clinical pathology, 1999-07, Vol.52 (7), p.529-531</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b478t-bb696457162c4679c6d0169ae5d7f5ac04836cd63d146cc5832c09b1c59e3f213</citedby><cites>FETCH-LOGICAL-b478t-bb696457162c4679c6d0169ae5d7f5ac04836cd63d146cc5832c09b1c59e3f213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC501497/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC501497/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1867621$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10605408$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Williams, D W</creatorcontrib><creatorcontrib>Walker, R</creatorcontrib><creatorcontrib>Lewis, M A</creatorcontrib><creatorcontrib>Allison, R T</creatorcontrib><creatorcontrib>Potts, A J</creatorcontrib><title>Adherence of Candida albicans to oral epithelial cells differentiated by Papanicolaou staining</title><title>Journal of clinical pathology</title><addtitle>J Clin Pathol</addtitle><description>OBJECTIVE: To examine the relative adherence of Candida albicans to oral epithelial cells differentiated by Papanicolaou staining. METHODS: Oral epithelial cells were collected from 10 healthy adults (five male, five female) and counted. Equal volumes of oral epithelial cells and candida were mixed and incubated. The epithelial cells from this mix were collected by filtration through 10 microns polycarbonate membrane filters. Cells retained on the membrane filters were stained with crystal violet followed by Papanicolaou stain. The number of yeast attached to each of 100 red, orange, and green staining oral epithelial cells was determined by direct microscopic examination. RESULTS: C albicans had a higher level of adherence (p < 0.001) to red staining oral epithelial cells (mean (SD) number of candida attached to 100 oral epithelial cells 562 (159)) than to cells staining either orange (105 (47)) or green (161 (66)). CONCLUSIONS: Oral epithelial cell variability for candidal adherence is confirmed. The technique provides an opportunity to examine the relation between oral epithelial cell type and oral candidosis in specific groups, such as tobacco smokers, where increased epithelial cell keratinisation and candidal colonisation has been reported.</description><subject>Adult</subject><subject>Analysis of Variance</subject><subject>Biological and medical sciences</subject><subject>Candida albicans - physiology</subject><subject>Cell Adhesion - physiology</subject><subject>Cells, Cultured</subject><subject>Coloring Agents</subject><subject>Ent and stomatologic mycoses</subject><subject>Epithelial Cells - microbiology</subject><subject>Female</subject><subject>Human mycoses</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Keratinocytes</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mouth Mucosa - cytology</subject><subject>Mouth Mucosa - microbiology</subject><subject>Mycoses</subject><subject>Specimen Handling - methods</subject><issn>0021-9746</issn><issn>1472-4146</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv1DAUhS0EotOBHWvkBYINGez4FS-6qCLKIFU8BHSJdWM7HQ-ZOMSZiv57PGRUyobNtaX7HfscHYSeUbKilMk3WzusRLlSeegHaEG5KgtOuXyIFoSUtNCKyxN0mtKWEMoUZY_RCSWSCE6qBfp-7jZ-9L31OLa4ht4FBxi6JljoE54ijiN02A9h2vgu5Kv1XZewC2170E0BJu9wc4s_wQB9sLGDuMdpgtCH_voJetRCl_zT47lE3y7efq3XxeXHd-_r88ui4aqaiqaRWnKhqCwtl0pb6QiVGrxwqhVgCa-YtE4yl4NZKypWWqIbaoX2rC0pW6Kz-d1h3-y8s9lYtm2GMexgvDURgvl304eNuY43RhDKtcr6l0f9GH_ufZrMLqRDUuh93CcjNZNSZxdL9HoG7RhTGn179wcl5tCHyX0YURqVh8748_u-7sFzARl4cQQgWejaEXob0l-ukkr-yVfMWEiT_3W3hvGHkYopYT5c1aa-KtX64vPafMn8q5lvdtv_O_wN6ouwgw</recordid><startdate>19990701</startdate><enddate>19990701</enddate><creator>Williams, D W</creator><creator>Walker, R</creator><creator>Lewis, M A</creator><creator>Allison, R T</creator><creator>Potts, A J</creator><general>BMJ Publishing Group Ltd and Association of Clinical Pathologists</general><general>BMJ</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19990701</creationdate><title>Adherence of Candida albicans to oral epithelial cells differentiated by Papanicolaou staining</title><author>Williams, D W ; Walker, R ; Lewis, M A ; Allison, R T ; Potts, A J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b478t-bb696457162c4679c6d0169ae5d7f5ac04836cd63d146cc5832c09b1c59e3f213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adult</topic><topic>Analysis of Variance</topic><topic>Biological and medical sciences</topic><topic>Candida albicans - physiology</topic><topic>Cell Adhesion - physiology</topic><topic>Cells, Cultured</topic><topic>Coloring Agents</topic><topic>Ent and stomatologic mycoses</topic><topic>Epithelial Cells - microbiology</topic><topic>Female</topic><topic>Human mycoses</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Keratinocytes</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mouth Mucosa - cytology</topic><topic>Mouth Mucosa - microbiology</topic><topic>Mycoses</topic><topic>Specimen Handling - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Williams, D W</creatorcontrib><creatorcontrib>Walker, R</creatorcontrib><creatorcontrib>Lewis, M A</creatorcontrib><creatorcontrib>Allison, R T</creatorcontrib><creatorcontrib>Potts, A J</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Williams, D W</au><au>Walker, R</au><au>Lewis, M A</au><au>Allison, R T</au><au>Potts, A J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adherence of Candida albicans to oral epithelial cells differentiated by Papanicolaou staining</atitle><jtitle>Journal of clinical pathology</jtitle><addtitle>J Clin Pathol</addtitle><date>1999-07-01</date><risdate>1999</risdate><volume>52</volume><issue>7</issue><spage>529</spage><epage>531</epage><pages>529-531</pages><issn>0021-9746</issn><eissn>1472-4146</eissn><coden>JCPAAK</coden><abstract>OBJECTIVE: To examine the relative adherence of Candida albicans to oral epithelial cells differentiated by Papanicolaou staining. METHODS: Oral epithelial cells were collected from 10 healthy adults (five male, five female) and counted. Equal volumes of oral epithelial cells and candida were mixed and incubated. The epithelial cells from this mix were collected by filtration through 10 microns polycarbonate membrane filters. Cells retained on the membrane filters were stained with crystal violet followed by Papanicolaou stain. The number of yeast attached to each of 100 red, orange, and green staining oral epithelial cells was determined by direct microscopic examination. RESULTS: C albicans had a higher level of adherence (p < 0.001) to red staining oral epithelial cells (mean (SD) number of candida attached to 100 oral epithelial cells 562 (159)) than to cells staining either orange (105 (47)) or green (161 (66)). CONCLUSIONS: Oral epithelial cell variability for candidal adherence is confirmed. The technique provides an opportunity to examine the relation between oral epithelial cell type and oral candidosis in specific groups, such as tobacco smokers, where increased epithelial cell keratinisation and candidal colonisation has been reported.</abstract><cop>London</cop><pub>BMJ Publishing Group Ltd and Association of Clinical Pathologists</pub><pmid>10605408</pmid><doi>10.1136/jcp.52.7.529</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Analysis of Variance Biological and medical sciences Candida albicans - physiology Cell Adhesion - physiology Cells, Cultured Coloring Agents Ent and stomatologic mycoses Epithelial Cells - microbiology Female Human mycoses Humans Infectious diseases Keratinocytes Male Medical sciences Mouth Mucosa - cytology Mouth Mucosa - microbiology Mycoses Specimen Handling - methods |
| title | Adherence of Candida albicans to oral epithelial cells differentiated by Papanicolaou staining |
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