Development of new restorer lines for CMS ogura system with the use of resynthesized oilseed rape (Brassica napus L.)
Resynthesized (RS) oilseed rape (Brassica napus L.) is potentially of great interest for hybrid breeding. However, a major problem with the direct use of RS B. napus is the quality of seed oil (high level of erucic acid) and seed meal (high glucosinolate content), which does not comply with double-l...
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| Veröffentlicht in: | Breeding Science 2016, Vol.66(4), pp.516-521 |
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| creator | Szała, Laurencja Sosnowska, Katarzyna Popławska, Wiesława Liersch, Alina Olejnik, Anna Kozłowska, Katarzyna Bocianowski, Jan Cegielska-Taras, Teresa |
| description | Resynthesized (RS) oilseed rape (Brassica napus L.) is potentially of great interest for hybrid breeding. However, a major problem with the direct use of RS B. napus is the quality of seed oil (high level of erucic acid) and seed meal (high glucosinolate content), which does not comply with double-low quality oilseed rape. Thus, additional developments are needed before RS B. napus can be introduced into breeding practice. In this study, RS oilseed rape was obtained through crosses between B. rapa ssp. chinensis var. chinensis and B. oleracea ssp. acephala var. sabellica. RS plant was then crossed with double-low (00) winter oilseed rape lines containing the Rfo gene for Ogura cytoplasmic male sterility (CMS ogu) system. Populations of doubled haploids (DH) were developed from these F1 hybrids using the microspore in vitro culture method. The seeds of semi-RS DH lines were analyzed for erucic acid and glucosinolate content. Among the populations of semi-RS DHs four 00-quality lines with the Rfo gene were selected. Using 344 AFLP markers to estimate genetic relatedness, we showed that the RS lines and semi-RS lines formed clusters that were clearly distinct from 96 winter oilseed rape parental lines of F1 hybrids. |
| doi_str_mv | 10.1270/jsbbs.15042 |
| format | Article |
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However, a major problem with the direct use of RS B. napus is the quality of seed oil (high level of erucic acid) and seed meal (high glucosinolate content), which does not comply with double-low quality oilseed rape. Thus, additional developments are needed before RS B. napus can be introduced into breeding practice. In this study, RS oilseed rape was obtained through crosses between B. rapa ssp. chinensis var. chinensis and B. oleracea ssp. acephala var. sabellica. RS plant was then crossed with double-low (00) winter oilseed rape lines containing the Rfo gene for Ogura cytoplasmic male sterility (CMS ogu) system. Populations of doubled haploids (DH) were developed from these F1 hybrids using the microspore in vitro culture method. The seeds of semi-RS DH lines were analyzed for erucic acid and glucosinolate content. Among the populations of semi-RS DHs four 00-quality lines with the Rfo gene were selected. Using 344 AFLP markers to estimate genetic relatedness, we showed that the RS lines and semi-RS lines formed clusters that were clearly distinct from 96 winter oilseed rape parental lines of F1 hybrids.</description><identifier>ISSN: 1344-7610</identifier><identifier>EISSN: 1347-3735</identifier><identifier>DOI: 10.1270/jsbbs.15042</identifier><identifier>PMID: 27795676</identifier><language>eng</language><publisher>Japan: Japanese Society of Breeding</publisher><subject>Acids ; Amplified fragment length polymorphism ; Brassica ; Brassica napus ; Breeding methods ; Clusters ; Cytoplasmic male sterility ; Genotype & phenotype ; hybrid breeding ; Hybrids ; In vitro methods and tests ; Male sterility ; Markers ; Oil ; Oilseeds ; Plant breeding ; Populations ; Quality ; Rape plants ; Rapeseed ; Research Paper ; resynthesis ; Seed meal ; Seeds ; semi-resynthesis ; Winter ; winter oilseed rape</subject><ispartof>Breeding Science, 2016, Vol.66(4), pp.516-521</ispartof><rights>2016 by JAPANESE SOCIETY OF BREEDING</rights><rights>Copyright Japan Science and Technology Agency 2016</rights><rights>Copyright © 2016 by JAPANESE SOCIETY OF BREEDING 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c533t-6f62daee46ce632e66073dcd20fe7724a9302808afb7d69278ea412e36cea16f3</citedby><cites>FETCH-LOGICAL-c533t-6f62daee46ce632e66073dcd20fe7724a9302808afb7d69278ea412e36cea16f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5010296/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5010296/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1877,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27795676$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Szała, Laurencja</creatorcontrib><creatorcontrib>Sosnowska, Katarzyna</creatorcontrib><creatorcontrib>Popławska, Wiesława</creatorcontrib><creatorcontrib>Liersch, Alina</creatorcontrib><creatorcontrib>Olejnik, Anna</creatorcontrib><creatorcontrib>Kozłowska, Katarzyna</creatorcontrib><creatorcontrib>Bocianowski, Jan</creatorcontrib><creatorcontrib>Cegielska-Taras, Teresa</creatorcontrib><title>Development of new restorer lines for CMS ogura system with the use of resynthesized oilseed rape (Brassica napus L.)</title><title>Breeding Science</title><addtitle>Breeding Science</addtitle><description>Resynthesized (RS) oilseed rape (Brassica napus L.) is potentially of great interest for hybrid breeding. However, a major problem with the direct use of RS B. napus is the quality of seed oil (high level of erucic acid) and seed meal (high glucosinolate content), which does not comply with double-low quality oilseed rape. Thus, additional developments are needed before RS B. napus can be introduced into breeding practice. In this study, RS oilseed rape was obtained through crosses between B. rapa ssp. chinensis var. chinensis and B. oleracea ssp. acephala var. sabellica. RS plant was then crossed with double-low (00) winter oilseed rape lines containing the Rfo gene for Ogura cytoplasmic male sterility (CMS ogu) system. Populations of doubled haploids (DH) were developed from these F1 hybrids using the microspore in vitro culture method. The seeds of semi-RS DH lines were analyzed for erucic acid and glucosinolate content. Among the populations of semi-RS DHs four 00-quality lines with the Rfo gene were selected. Using 344 AFLP markers to estimate genetic relatedness, we showed that the RS lines and semi-RS lines formed clusters that were clearly distinct from 96 winter oilseed rape parental lines of F1 hybrids.</description><subject>Acids</subject><subject>Amplified fragment length polymorphism</subject><subject>Brassica</subject><subject>Brassica napus</subject><subject>Breeding methods</subject><subject>Clusters</subject><subject>Cytoplasmic male sterility</subject><subject>Genotype & phenotype</subject><subject>hybrid breeding</subject><subject>Hybrids</subject><subject>In vitro methods and tests</subject><subject>Male sterility</subject><subject>Markers</subject><subject>Oil</subject><subject>Oilseeds</subject><subject>Plant breeding</subject><subject>Populations</subject><subject>Quality</subject><subject>Rape plants</subject><subject>Rapeseed</subject><subject>Research Paper</subject><subject>resynthesis</subject><subject>Seed meal</subject><subject>Seeds</subject><subject>semi-resynthesis</subject><subject>Winter</subject><subject>winter oilseed rape</subject><issn>1344-7610</issn><issn>1347-3735</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNpdkU1v1DAQhi1ERUvhxB1Z4lKEsvgjsZMLUlmgRVrEAThb3mSy61Vip56k1fLr6_1gBVxmxppnXs34JeQVZzMuNHu_weUSZ7xguXhCLrjMdSa1LJ7u6zzTirNz8hxxw5hIUPGMnAutq0JpdUGmT3APXRh68CMNLfXwQCPgGCJE2jkPSNsQ6fzbDxpWU7QUtzhCTx_cuKbjGuiEsJtLM1uf3uh-Q0OD6xBSjnYAevUxWkRXW-rtMCFdzN6-IGetTcjLY74kv758_jm_zRbfb77OrxdZXUg5ZqpVorEAuapBSQFKMS2buhGsBa1FbivJRMlK2y51oyqhS7A5FyATb7lq5SX5cNAdpmUPTZ2OjLYzQ3S9jVsTrDP_drxbm1W4NwXjTFQqCVwdBWK4m9K_mN5hDV1nPYQJDS9lwZhUpUjom__QTZiiT-ftKM2UqLRM1LsDVceAGKE9LcOZ2dlp9naavZ2Jfv33_if2j38JuD4AGxztCk6AjaOrOziKKWXyXdiLnnr12kYDXj4C3FO03Q</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>Szała, Laurencja</creator><creator>Sosnowska, Katarzyna</creator><creator>Popławska, Wiesława</creator><creator>Liersch, Alina</creator><creator>Olejnik, Anna</creator><creator>Kozłowska, Katarzyna</creator><creator>Bocianowski, Jan</creator><creator>Cegielska-Taras, Teresa</creator><general>Japanese Society of Breeding</general><general>Japan Science and Technology Agency</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2016</creationdate><title>Development of new restorer lines for CMS ogura system with the use of resynthesized oilseed rape (Brassica napus L.)</title><author>Szała, Laurencja ; Sosnowska, Katarzyna ; Popławska, Wiesława ; Liersch, Alina ; Olejnik, Anna ; Kozłowska, Katarzyna ; Bocianowski, Jan ; Cegielska-Taras, Teresa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c533t-6f62daee46ce632e66073dcd20fe7724a9302808afb7d69278ea412e36cea16f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Acids</topic><topic>Amplified fragment length polymorphism</topic><topic>Brassica</topic><topic>Brassica napus</topic><topic>Breeding methods</topic><topic>Clusters</topic><topic>Cytoplasmic male sterility</topic><topic>Genotype & phenotype</topic><topic>hybrid breeding</topic><topic>Hybrids</topic><topic>In vitro methods and tests</topic><topic>Male sterility</topic><topic>Markers</topic><topic>Oil</topic><topic>Oilseeds</topic><topic>Plant breeding</topic><topic>Populations</topic><topic>Quality</topic><topic>Rape plants</topic><topic>Rapeseed</topic><topic>Research Paper</topic><topic>resynthesis</topic><topic>Seed meal</topic><topic>Seeds</topic><topic>semi-resynthesis</topic><topic>Winter</topic><topic>winter oilseed rape</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Szała, Laurencja</creatorcontrib><creatorcontrib>Sosnowska, Katarzyna</creatorcontrib><creatorcontrib>Popławska, Wiesława</creatorcontrib><creatorcontrib>Liersch, Alina</creatorcontrib><creatorcontrib>Olejnik, Anna</creatorcontrib><creatorcontrib>Kozłowska, Katarzyna</creatorcontrib><creatorcontrib>Bocianowski, Jan</creatorcontrib><creatorcontrib>Cegielska-Taras, Teresa</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Breeding Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Szała, Laurencja</au><au>Sosnowska, Katarzyna</au><au>Popławska, Wiesława</au><au>Liersch, Alina</au><au>Olejnik, Anna</au><au>Kozłowska, Katarzyna</au><au>Bocianowski, Jan</au><au>Cegielska-Taras, Teresa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of new restorer lines for CMS ogura system with the use of resynthesized oilseed rape (Brassica napus L.)</atitle><jtitle>Breeding Science</jtitle><addtitle>Breeding Science</addtitle><date>2016</date><risdate>2016</risdate><volume>66</volume><issue>4</issue><spage>516</spage><epage>521</epage><pages>516-521</pages><issn>1344-7610</issn><eissn>1347-3735</eissn><abstract>Resynthesized (RS) oilseed rape (Brassica napus L.) is potentially of great interest for hybrid breeding. However, a major problem with the direct use of RS B. napus is the quality of seed oil (high level of erucic acid) and seed meal (high glucosinolate content), which does not comply with double-low quality oilseed rape. Thus, additional developments are needed before RS B. napus can be introduced into breeding practice. In this study, RS oilseed rape was obtained through crosses between B. rapa ssp. chinensis var. chinensis and B. oleracea ssp. acephala var. sabellica. RS plant was then crossed with double-low (00) winter oilseed rape lines containing the Rfo gene for Ogura cytoplasmic male sterility (CMS ogu) system. Populations of doubled haploids (DH) were developed from these F1 hybrids using the microspore in vitro culture method. The seeds of semi-RS DH lines were analyzed for erucic acid and glucosinolate content. Among the populations of semi-RS DHs four 00-quality lines with the Rfo gene were selected. Using 344 AFLP markers to estimate genetic relatedness, we showed that the RS lines and semi-RS lines formed clusters that were clearly distinct from 96 winter oilseed rape parental lines of F1 hybrids.</abstract><cop>Japan</cop><pub>Japanese Society of Breeding</pub><pmid>27795676</pmid><doi>10.1270/jsbbs.15042</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Breeding Science, 2016, Vol.66(4), pp.516-521 |
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| language | eng |
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| source | J-STAGE Free; PubMed Central; EZB Electronic Journals Library |
| subjects | Acids Amplified fragment length polymorphism Brassica Brassica napus Breeding methods Clusters Cytoplasmic male sterility Genotype & phenotype hybrid breeding Hybrids In vitro methods and tests Male sterility Markers Oil Oilseeds Plant breeding Populations Quality Rape plants Rapeseed Research Paper resynthesis Seed meal Seeds semi-resynthesis Winter winter oilseed rape |
| title | Development of new restorer lines for CMS ogura system with the use of resynthesized oilseed rape (Brassica napus L.) |
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