Serological response of patients infected with Salmonella typhi
AIMS: To evaluate a rapid immunoblotting procedure for providing evidence of infection with Salmonella typhi using 73 sera from patients infected with S typhi. METHODS: A sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE)/immunoblotting procedure using lipopolysaccharide (LPS, O =...
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| Veröffentlicht in: | Journal of clinical pathology 1997-11, Vol.50 (11), p.944-946 |
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| container_title | Journal of clinical pathology |
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| creator | Chart, H Rowe, B Cheesbrough, J S |
| description | AIMS: To evaluate a rapid immunoblotting procedure for providing evidence of infection with Salmonella typhi using 73 sera from patients infected with S typhi. METHODS: A sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE)/immunoblotting procedure using lipopolysaccharide (LPS, O = 9,12) and flagellar (H = d) antigens was used. RESULTS: Seventy two of 73 sera contained antibodies to LPS, 40 sera also contained antibodies to H = d flagellar antigens. Analysis of acute and convalescent sera showed that only 62% of patients produced antibodies to flagellar antigens. CONCLUSIONS: The SDS-PAGE/immunoblotting procedure provided a rapid method for providing serological evidence of infection with S typhi. |
| doi_str_mv | 10.1136/jcp.50.11.944 |
| format | Article |
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METHODS: A sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE)/immunoblotting procedure using lipopolysaccharide (LPS, O = 9,12) and flagellar (H = d) antigens was used. RESULTS: Seventy two of 73 sera contained antibodies to LPS, 40 sera also contained antibodies to H = d flagellar antigens. Analysis of acute and convalescent sera showed that only 62% of patients produced antibodies to flagellar antigens. CONCLUSIONS: The SDS-PAGE/immunoblotting procedure provided a rapid method for providing serological evidence of infection with S typhi.</description><identifier>ISSN: 0021-9746</identifier><identifier>EISSN: 1472-4146</identifier><identifier>DOI: 10.1136/jcp.50.11.944</identifier><identifier>PMID: 9462246</identifier><identifier>CODEN: JCPAAK</identifier><language>eng</language><publisher>London: BMJ Publishing Group Ltd and Association of Clinical Pathologists</publisher><subject>Acute Disease ; Adolescent ; Adult ; Antibodies, Bacterial - blood ; Antigens, Bacterial - immunology ; Bacterial diseases ; Bacterial diseases of the digestive system and abdomen ; Biological and medical sciences ; Biomarkers - blood ; Electrophoresis, Polyacrylamide Gel ; Evaluation Studies as Topic ; Female ; Flagella - immunology ; Human bacterial diseases ; Humans ; Immunoblotting ; Infectious diseases ; Lipopolysaccharides - immunology ; Male ; Medical sciences ; Middle Aged ; Salmonella enteritidis - immunology ; Salmonella typhi - immunology ; Typhoid Fever - diagnosis ; Typhoid Fever - immunology</subject><ispartof>Journal of clinical pathology, 1997-11, Vol.50 (11), p.944-946</ispartof><rights>1998 INIST-CNRS</rights><rights>Copyright BMJ Publishing Group LTD Nov 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b509t-19b3734d67c96572c8e94e94bde83f3d3114f1942fab47f3654509ca87a7acaa3</citedby><cites>FETCH-LOGICAL-b509t-19b3734d67c96572c8e94e94bde83f3d3114f1942fab47f3654509ca87a7acaa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC500321/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC500321/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2077142$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9462246$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chart, H</creatorcontrib><creatorcontrib>Rowe, B</creatorcontrib><creatorcontrib>Cheesbrough, J S</creatorcontrib><title>Serological response of patients infected with Salmonella typhi</title><title>Journal of clinical pathology</title><addtitle>J Clin Pathol</addtitle><description>AIMS: To evaluate a rapid immunoblotting procedure for providing evidence of infection with Salmonella typhi using 73 sera from patients infected with S typhi. METHODS: A sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE)/immunoblotting procedure using lipopolysaccharide (LPS, O = 9,12) and flagellar (H = d) antigens was used. RESULTS: Seventy two of 73 sera contained antibodies to LPS, 40 sera also contained antibodies to H = d flagellar antigens. Analysis of acute and convalescent sera showed that only 62% of patients produced antibodies to flagellar antigens. CONCLUSIONS: The SDS-PAGE/immunoblotting procedure provided a rapid method for providing serological evidence of infection with S typhi.</description><subject>Acute Disease</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antigens, Bacterial - immunology</subject><subject>Bacterial diseases</subject><subject>Bacterial diseases of the digestive system and abdomen</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - blood</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Evaluation Studies as Topic</subject><subject>Female</subject><subject>Flagella - immunology</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Infectious diseases</subject><subject>Lipopolysaccharides - immunology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Salmonella enteritidis - immunology</subject><subject>Salmonella typhi - immunology</subject><subject>Typhoid Fever - diagnosis</subject><subject>Typhoid Fever - immunology</subject><issn>0021-9746</issn><issn>1472-4146</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkc9rFDEUx0NR6rb16FEYUEovs83vTA5FZLW2UGplqwcvIZNJullnJmMyq-1_3yy7rNWLEHgPvp-X9318AXiF4BQhwk-XZpiydT-VlO6BCaIClxRR_gxMIMSolILyF-AgpSWEiAhE9sG-pBxjyifg3dzG0IY7b3RbRJuG0CdbBFcMevS2H1Phe2fNaJvitx8XxVy3Xeht2-pifBgW_gg8d7pN9uW2HoKv5x9vZxfl1edPl7P3V2XNoBxLJGsiCG24MJIzgU1lJc2vbmxFHGkIQtQhSbHTNRWOcEbznNGV0EIbrckhONv8O6zqzjYmW4u6VUP0nY4PKmiv_lZ6v1B34ZdiEBKM8vzxdj6GnyubRtX5ZNZ39DaskhKSUUEIz-Cbf8BlWMU-36aQEJCyijCYqXJDmRhSitbtnCCo1rGoHEvenXuVY8n866f2d_Q2h6y_3eo65SRc1L3xaYdhKASi-M9an0Z7v5N1_KG4IIKp628z9f1mXt3wD1_Umj_Z8HW3_I_DR2VOsZs</recordid><startdate>19971101</startdate><enddate>19971101</enddate><creator>Chart, H</creator><creator>Rowe, B</creator><creator>Cheesbrough, J S</creator><general>BMJ Publishing Group Ltd and Association of Clinical Pathologists</general><general>BMJ</general><general>BMJ Publishing Group LTD</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19971101</creationdate><title>Serological response of patients infected with Salmonella typhi</title><author>Chart, H ; 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| subjects | Acute Disease Adolescent Adult Antibodies, Bacterial - blood Antigens, Bacterial - immunology Bacterial diseases Bacterial diseases of the digestive system and abdomen Biological and medical sciences Biomarkers - blood Electrophoresis, Polyacrylamide Gel Evaluation Studies as Topic Female Flagella - immunology Human bacterial diseases Humans Immunoblotting Infectious diseases Lipopolysaccharides - immunology Male Medical sciences Middle Aged Salmonella enteritidis - immunology Salmonella typhi - immunology Typhoid Fever - diagnosis Typhoid Fever - immunology |
| title | Serological response of patients infected with Salmonella typhi |
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