Detection of Peste des Petits Ruminants Viral RNA in Fecal Samples of Goats after an Outbreak in Punjab Province of Pakistan: A Longitudinal Study
Peste des petits ruminants (PPR) is a highly contagious viral disease of domestic and wild small ruminants and thus has serious socioeconomic implications. In Pakistan, during the year 2012-2013, estimated losses due to PPR were worth Rs. 31.51 billions. Close contact between infected and susceptibl...
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| Veröffentlicht in: | BioMed research international 2016-01, Vol.2016 (2016), p.1-5 |
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| creator | Qureshi, Zafar-ul-Ahsan Rashid Malik, Adnan Afzal, Muhammad Irshad, Hamid Iqbal Dasti, Javid Latif, Asma Bin Zahur, Aamer Wasee Ullah, Riasat Rasheed, Tahir |
| description | Peste des petits ruminants (PPR) is a highly contagious viral disease of domestic and wild small ruminants and thus has serious socioeconomic implications. In Pakistan, during the year 2012-2013, estimated losses due to PPR were worth Rs. 31.51 billions. Close contact between infected and susceptible animals is an important route of transmission of PPR. Therefore, carrier animals play an important role in unnoticed transmission of PPR. The objective of the study was to investigate the detection of PPR virus in goats recovered from PPR. A suspected PPR outbreak was investigated and confirmed as PPR after analysing appropriate samples collected from infected animals using rRT-PCR. A longitudinal study was conducted over the period of 16 weeks to ascertain the detection of PPR virus (PPRV) in faecal samples of recovered goats. Ninety-six (96) faecal samples from each sampling were collected at 4, 8, 12, and 16 weeks after the outbreak. Faecal samples were analysed using rRT-PCR. Of 96 from each sampling a total of 46, 37, 29, and 25 samples were positive for PPR viral genome at 4, 8, 12, and 16 weeks, respectively, after recovery. Attempts were made for the isolation of PPR virus on Vero cells, but results were negative. These results indicated the detection of PPR viral RNA up to 16 weeks after infection. Therefore, these results may help in the future epidemiology of PPR virus shedding and possible role as source of silent infection for healthy animals especially when there is no history of any outbreak in nearby flock or area. |
| doi_str_mv | 10.1155/2016/1486824 |
| format | Article |
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In Pakistan, during the year 2012-2013, estimated losses due to PPR were worth Rs. 31.51 billions. Close contact between infected and susceptible animals is an important route of transmission of PPR. Therefore, carrier animals play an important role in unnoticed transmission of PPR. The objective of the study was to investigate the detection of PPR virus in goats recovered from PPR. A suspected PPR outbreak was investigated and confirmed as PPR after analysing appropriate samples collected from infected animals using rRT-PCR. A longitudinal study was conducted over the period of 16 weeks to ascertain the detection of PPR virus (PPRV) in faecal samples of recovered goats. Ninety-six (96) faecal samples from each sampling were collected at 4, 8, 12, and 16 weeks after the outbreak. Faecal samples were analysed using rRT-PCR. Of 96 from each sampling a total of 46, 37, 29, and 25 samples were positive for PPR viral genome at 4, 8, 12, and 16 weeks, respectively, after recovery. Attempts were made for the isolation of PPR virus on Vero cells, but results were negative. These results indicated the detection of PPR viral RNA up to 16 weeks after infection. Therefore, these results may help in the future epidemiology of PPR virus shedding and possible role as source of silent infection for healthy animals especially when there is no history of any outbreak in nearby flock or area.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2016/1486824</identifier><identifier>PMID: 27597951</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Animal behavior ; Animal diseases ; Animals ; Antigens ; Disease Outbreaks - statistics & numerical data ; Disease Outbreaks - veterinary ; Diseases ; Epidemiology ; Feces ; Feces - virology ; Female ; Genetic aspects ; Goat Diseases - diagnosis ; Goat Diseases - epidemiology ; Goat Diseases - virology ; Goats ; Goats - virology ; Health aspects ; Immunization ; Laboratories ; Male ; Pakistan - epidemiology ; Peste-des-Petits-Ruminants - diagnosis ; Peste-des-Petits-Ruminants - epidemiology ; Peste-des-Petits-Ruminants - virology ; Peste-des-petits-ruminants virus - isolation & purification ; Pneumonia ; Population Surveillance - methods ; Prevalence ; Risk Assessment - methods ; Ruminantia ; Studies</subject><ispartof>BioMed research international, 2016-01, Vol.2016 (2016), p.1-5</ispartof><rights>Copyright © 2016 Riasat Wasee Ullah et al.</rights><rights>COPYRIGHT 2016 John Wiley & Sons, Inc.</rights><rights>Copyright © 2016 Riasat Wasee Ullah et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2016 Riasat Wasee Ullah et al. 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-636c1f5692035beb1550a7456fc15c85338748bbaad26e4baae6bf2cee7ea6d43</citedby><cites>FETCH-LOGICAL-c532t-636c1f5692035beb1550a7456fc15c85338748bbaad26e4baae6bf2cee7ea6d43</cites><orcidid>0000-0001-5016-8969</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5002454/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5002454/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27597951$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Atmaca, Hasan T.</contributor><creatorcontrib>Qureshi, Zafar-ul-Ahsan</creatorcontrib><creatorcontrib>Rashid Malik, Adnan</creatorcontrib><creatorcontrib>Afzal, Muhammad</creatorcontrib><creatorcontrib>Irshad, Hamid</creatorcontrib><creatorcontrib>Iqbal Dasti, Javid</creatorcontrib><creatorcontrib>Latif, Asma</creatorcontrib><creatorcontrib>Bin Zahur, Aamer</creatorcontrib><creatorcontrib>Wasee Ullah, Riasat</creatorcontrib><creatorcontrib>Rasheed, Tahir</creatorcontrib><title>Detection of Peste des Petits Ruminants Viral RNA in Fecal Samples of Goats after an Outbreak in Punjab Province of Pakistan: A Longitudinal Study</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Peste des petits ruminants (PPR) is a highly contagious viral disease of domestic and wild small ruminants and thus has serious socioeconomic implications. In Pakistan, during the year 2012-2013, estimated losses due to PPR were worth Rs. 31.51 billions. Close contact between infected and susceptible animals is an important route of transmission of PPR. Therefore, carrier animals play an important role in unnoticed transmission of PPR. The objective of the study was to investigate the detection of PPR virus in goats recovered from PPR. A suspected PPR outbreak was investigated and confirmed as PPR after analysing appropriate samples collected from infected animals using rRT-PCR. A longitudinal study was conducted over the period of 16 weeks to ascertain the detection of PPR virus (PPRV) in faecal samples of recovered goats. Ninety-six (96) faecal samples from each sampling were collected at 4, 8, 12, and 16 weeks after the outbreak. Faecal samples were analysed using rRT-PCR. Of 96 from each sampling a total of 46, 37, 29, and 25 samples were positive for PPR viral genome at 4, 8, 12, and 16 weeks, respectively, after recovery. Attempts were made for the isolation of PPR virus on Vero cells, but results were negative. These results indicated the detection of PPR viral RNA up to 16 weeks after infection. Therefore, these results may help in the future epidemiology of PPR virus shedding and possible role as source of silent infection for healthy animals especially when there is no history of any outbreak in nearby flock or area.</description><subject>Animal behavior</subject><subject>Animal diseases</subject><subject>Animals</subject><subject>Antigens</subject><subject>Disease Outbreaks - statistics & numerical data</subject><subject>Disease Outbreaks - veterinary</subject><subject>Diseases</subject><subject>Epidemiology</subject><subject>Feces</subject><subject>Feces - virology</subject><subject>Female</subject><subject>Genetic aspects</subject><subject>Goat Diseases - diagnosis</subject><subject>Goat Diseases - epidemiology</subject><subject>Goat Diseases - virology</subject><subject>Goats</subject><subject>Goats - virology</subject><subject>Health aspects</subject><subject>Immunization</subject><subject>Laboratories</subject><subject>Male</subject><subject>Pakistan - epidemiology</subject><subject>Peste-des-Petits-Ruminants - 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In Pakistan, during the year 2012-2013, estimated losses due to PPR were worth Rs. 31.51 billions. Close contact between infected and susceptible animals is an important route of transmission of PPR. Therefore, carrier animals play an important role in unnoticed transmission of PPR. The objective of the study was to investigate the detection of PPR virus in goats recovered from PPR. A suspected PPR outbreak was investigated and confirmed as PPR after analysing appropriate samples collected from infected animals using rRT-PCR. A longitudinal study was conducted over the period of 16 weeks to ascertain the detection of PPR virus (PPRV) in faecal samples of recovered goats. Ninety-six (96) faecal samples from each sampling were collected at 4, 8, 12, and 16 weeks after the outbreak. Faecal samples were analysed using rRT-PCR. Of 96 from each sampling a total of 46, 37, 29, and 25 samples were positive for PPR viral genome at 4, 8, 12, and 16 weeks, respectively, after recovery. Attempts were made for the isolation of PPR virus on Vero cells, but results were negative. These results indicated the detection of PPR viral RNA up to 16 weeks after infection. Therefore, these results may help in the future epidemiology of PPR virus shedding and possible role as source of silent infection for healthy animals especially when there is no history of any outbreak in nearby flock or area.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>27597951</pmid><doi>10.1155/2016/1486824</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0001-5016-8969</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animal behavior Animal diseases Animals Antigens Disease Outbreaks - statistics & numerical data Disease Outbreaks - veterinary Diseases Epidemiology Feces Feces - virology Female Genetic aspects Goat Diseases - diagnosis Goat Diseases - epidemiology Goat Diseases - virology Goats Goats - virology Health aspects Immunization Laboratories Male Pakistan - epidemiology Peste-des-Petits-Ruminants - diagnosis Peste-des-Petits-Ruminants - epidemiology Peste-des-Petits-Ruminants - virology Peste-des-petits-ruminants virus - isolation & purification Pneumonia Population Surveillance - methods Prevalence Risk Assessment - methods Ruminantia Studies |
| title | Detection of Peste des Petits Ruminants Viral RNA in Fecal Samples of Goats after an Outbreak in Punjab Province of Pakistan: A Longitudinal Study |
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