Comparative Proteomic Analysis of Buffalo Oocytes Matured in vitro Using iTRAQ Technique
To investigate the protein profiling of buffalo oocytes at the germinal vesicle (GV) stage and metaphase II (MII) stage, an iTRAQ-based strategy was applied. A total of 3,763 proteins were identified, which representing the largest buffalo oocytes proteome dataset to date. Among these proteins ident...
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| Veröffentlicht in: | Scientific reports 2016-08, Vol.6 (1), p.31795-31795, Article 31795 |
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| description | To investigate the protein profiling of buffalo oocytes at the germinal vesicle (GV) stage and metaphase II (MII) stage, an iTRAQ-based strategy was applied. A total of 3,763 proteins were identified, which representing the largest buffalo oocytes proteome dataset to date. Among these proteins identified, 173 proteins were differentially expressed in GV oocytes and competent MII oocytes and 146 proteins were differentially abundant in competent and incompetent matured oocytes. Functional and KEGG pathway analysis revealed that the up-regulated proteins in competent MII oocytes were related to chromosome segregation, microtubule-based process, protein transport, oxidation reduction, ribosome and oxidative phosphorylation, etc., in comparison with GV and incompetent MII oocytes. This is the first proteomic report on buffalo oocytes from different maturation stages and developmental competent status. These data will provide valuable information for understanding the molecular mechanism underlying buffalo oocyte maturation and these proteins may potentially act as markers to predict developmental competence of buffalo oocyte during
in vitro
maturation. |
| doi_str_mv | 10.1038/srep31795 |
| format | Article |
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in vitro
maturation.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep31795</identifier><identifier>PMID: 27561356</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/136/1455 ; 631/601/1737 ; 82/16 ; 82/29 ; Animals ; Buffaloes ; Catalysis ; Cattle ; Cell Culture Techniques ; Computational Biology ; Cumulus Cells - metabolism ; Female ; Gene Expression Profiling ; Humanities and Social Sciences ; Mass Spectrometry ; Maturation ; Metaphase ; Microtubules - metabolism ; multidisciplinary ; Oocytes ; Oocytes - metabolism ; Oogenesis ; Oxidation ; Oxidative Phosphorylation ; Peptides ; Phosphorylation ; Protein transport ; Proteins ; Proteome ; Proteomics - methods ; Ribosomes - metabolism ; Science ; Trypsin - chemistry</subject><ispartof>Scientific reports, 2016-08, Vol.6 (1), p.31795-31795, Article 31795</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Nature Publishing Group Aug 2016</rights><rights>Copyright © 2016, The Author(s) 2016 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-5304bc6558bafa98f09cc05096b31cd86fe054536b5968c5c484c91387a280693</citedby><cites>FETCH-LOGICAL-c438t-5304bc6558bafa98f09cc05096b31cd86fe054536b5968c5c484c91387a280693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4999887/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4999887/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27561356$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Lingsheng</creatorcontrib><creatorcontrib>Zhai, Linhui</creatorcontrib><creatorcontrib>Qu, Chunfeng</creatorcontrib><creatorcontrib>Zhang, Chengpu</creatorcontrib><creatorcontrib>Li, Sheng</creatorcontrib><creatorcontrib>Wu, Feilin</creatorcontrib><creatorcontrib>Qi, Yingzi</creatorcontrib><creatorcontrib>Lu, Fenghua</creatorcontrib><creatorcontrib>Xu, Ping</creatorcontrib><creatorcontrib>Li, Xiangping</creatorcontrib><creatorcontrib>Shi, Deshun</creatorcontrib><title>Comparative Proteomic Analysis of Buffalo Oocytes Matured in vitro Using iTRAQ Technique</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>To investigate the protein profiling of buffalo oocytes at the germinal vesicle (GV) stage and metaphase II (MII) stage, an iTRAQ-based strategy was applied. A total of 3,763 proteins were identified, which representing the largest buffalo oocytes proteome dataset to date. Among these proteins identified, 173 proteins were differentially expressed in GV oocytes and competent MII oocytes and 146 proteins were differentially abundant in competent and incompetent matured oocytes. Functional and KEGG pathway analysis revealed that the up-regulated proteins in competent MII oocytes were related to chromosome segregation, microtubule-based process, protein transport, oxidation reduction, ribosome and oxidative phosphorylation, etc., in comparison with GV and incompetent MII oocytes. This is the first proteomic report on buffalo oocytes from different maturation stages and developmental competent status. These data will provide valuable information for understanding the molecular mechanism underlying buffalo oocyte maturation and these proteins may potentially act as markers to predict developmental competence of buffalo oocyte during
in vitro
maturation.</description><subject>631/136/1455</subject><subject>631/601/1737</subject><subject>82/16</subject><subject>82/29</subject><subject>Animals</subject><subject>Buffaloes</subject><subject>Catalysis</subject><subject>Cattle</subject><subject>Cell Culture Techniques</subject><subject>Computational Biology</subject><subject>Cumulus Cells - metabolism</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Humanities and Social Sciences</subject><subject>Mass Spectrometry</subject><subject>Maturation</subject><subject>Metaphase</subject><subject>Microtubules - metabolism</subject><subject>multidisciplinary</subject><subject>Oocytes</subject><subject>Oocytes - metabolism</subject><subject>Oogenesis</subject><subject>Oxidation</subject><subject>Oxidative Phosphorylation</subject><subject>Peptides</subject><subject>Phosphorylation</subject><subject>Protein transport</subject><subject>Proteins</subject><subject>Proteome</subject><subject>Proteomics - methods</subject><subject>Ribosomes - metabolism</subject><subject>Science</subject><subject>Trypsin - chemistry</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkUtrGzEUhUVJaEziRf9AEWTTFNzobWkTcEweBQenxYHuhEbW2DIzI0eaMfjfV4lT4zR3owvn09FBB4AvGP3AiMrLFN2a4qHin0CPIMYHhBJydLCfgH5KK5SHE8Ww-gxOyJALTLnogT_jUK9NNK3fOPgYQ-tC7S0cNabaJp9gKOF1V5amCnAa7LZ1CT6YtotuDn0DN76NAT4l3yygn_0e_YIzZ5eNf-7cGTjOt5Lrv52n4On2Zja-H0ymdz_Ho8nAMirbAaeIFVZwLgtTGiVLpKxFHClRUGznUpQOccapKLgS0nLLJLMKUzk0RCKh6Cm42vmuu6J2c-uaNppKr6OvTdzqYLx-rzR-qRdho5lSSsphNvj2ZhBDzp1aXftkXVWZxoUuaSwxE5IwxTN6_h-6Cl3MX_VCKSkYQa_UxY6yMaTcTrkPg5F-qUzvK8vs18P0e_JfQRn4vgNSlpqFiwdPfnD7C5lMn48</recordid><startdate>20160826</startdate><enddate>20160826</enddate><creator>Chen, Lingsheng</creator><creator>Zhai, Linhui</creator><creator>Qu, Chunfeng</creator><creator>Zhang, Chengpu</creator><creator>Li, Sheng</creator><creator>Wu, Feilin</creator><creator>Qi, Yingzi</creator><creator>Lu, Fenghua</creator><creator>Xu, Ping</creator><creator>Li, Xiangping</creator><creator>Shi, Deshun</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160826</creationdate><title>Comparative Proteomic Analysis of Buffalo Oocytes Matured in vitro Using iTRAQ Technique</title><author>Chen, Lingsheng ; Zhai, Linhui ; Qu, Chunfeng ; Zhang, Chengpu ; Li, Sheng ; Wu, Feilin ; Qi, Yingzi ; Lu, Fenghua ; Xu, Ping ; Li, Xiangping ; Shi, Deshun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-5304bc6558bafa98f09cc05096b31cd86fe054536b5968c5c484c91387a280693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>631/136/1455</topic><topic>631/601/1737</topic><topic>82/16</topic><topic>82/29</topic><topic>Animals</topic><topic>Buffaloes</topic><topic>Catalysis</topic><topic>Cattle</topic><topic>Cell Culture Techniques</topic><topic>Computational Biology</topic><topic>Cumulus Cells - metabolism</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Humanities and Social Sciences</topic><topic>Mass Spectrometry</topic><topic>Maturation</topic><topic>Metaphase</topic><topic>Microtubules - metabolism</topic><topic>multidisciplinary</topic><topic>Oocytes</topic><topic>Oocytes - metabolism</topic><topic>Oogenesis</topic><topic>Oxidation</topic><topic>Oxidative Phosphorylation</topic><topic>Peptides</topic><topic>Phosphorylation</topic><topic>Protein transport</topic><topic>Proteins</topic><topic>Proteome</topic><topic>Proteomics - methods</topic><topic>Ribosomes - metabolism</topic><topic>Science</topic><topic>Trypsin - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Lingsheng</creatorcontrib><creatorcontrib>Zhai, Linhui</creatorcontrib><creatorcontrib>Qu, Chunfeng</creatorcontrib><creatorcontrib>Zhang, Chengpu</creatorcontrib><creatorcontrib>Li, Sheng</creatorcontrib><creatorcontrib>Wu, Feilin</creatorcontrib><creatorcontrib>Qi, Yingzi</creatorcontrib><creatorcontrib>Lu, Fenghua</creatorcontrib><creatorcontrib>Xu, Ping</creatorcontrib><creatorcontrib>Li, Xiangping</creatorcontrib><creatorcontrib>Shi, Deshun</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Lingsheng</au><au>Zhai, Linhui</au><au>Qu, Chunfeng</au><au>Zhang, Chengpu</au><au>Li, Sheng</au><au>Wu, Feilin</au><au>Qi, Yingzi</au><au>Lu, Fenghua</au><au>Xu, Ping</au><au>Li, Xiangping</au><au>Shi, Deshun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative Proteomic Analysis of Buffalo Oocytes Matured in vitro Using iTRAQ Technique</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-08-26</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>31795</spage><epage>31795</epage><pages>31795-31795</pages><artnum>31795</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>To investigate the protein profiling of buffalo oocytes at the germinal vesicle (GV) stage and metaphase II (MII) stage, an iTRAQ-based strategy was applied. A total of 3,763 proteins were identified, which representing the largest buffalo oocytes proteome dataset to date. Among these proteins identified, 173 proteins were differentially expressed in GV oocytes and competent MII oocytes and 146 proteins were differentially abundant in competent and incompetent matured oocytes. Functional and KEGG pathway analysis revealed that the up-regulated proteins in competent MII oocytes were related to chromosome segregation, microtubule-based process, protein transport, oxidation reduction, ribosome and oxidative phosphorylation, etc., in comparison with GV and incompetent MII oocytes. This is the first proteomic report on buffalo oocytes from different maturation stages and developmental competent status. These data will provide valuable information for understanding the molecular mechanism underlying buffalo oocyte maturation and these proteins may potentially act as markers to predict developmental competence of buffalo oocyte during
in vitro
maturation.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27561356</pmid><doi>10.1038/srep31795</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/136/1455 631/601/1737 82/16 82/29 Animals Buffaloes Catalysis Cattle Cell Culture Techniques Computational Biology Cumulus Cells - metabolism Female Gene Expression Profiling Humanities and Social Sciences Mass Spectrometry Maturation Metaphase Microtubules - metabolism multidisciplinary Oocytes Oocytes - metabolism Oogenesis Oxidation Oxidative Phosphorylation Peptides Phosphorylation Protein transport Proteins Proteome Proteomics - methods Ribosomes - metabolism Science Trypsin - chemistry |
| title | Comparative Proteomic Analysis of Buffalo Oocytes Matured in vitro Using iTRAQ Technique |
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