Standardisation of polymerase chain reaction for the detection of Salmonella typhi in typhoid fever

To improve the diagnosis of Salmonella typhi infection, a polymerase chain reaction (PCR) assay was developed for the amplification of the dH flagellin gene of S typhi. Primers were designed from dH flagellin gene sequence which will give an amplification product of 486 base pairs. In tests to study...

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Veröffentlicht in:	Journal of clinical pathology 1997-05, Vol.50 (5), p.437-439
Hauptverfasser:	Chaudhry, R, Laxmi, B V, Nisar, N, Ray, K, Kumar, D
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences DNA, Bacterial - blood Humans Investigative techniques, diagnostic techniques (general aspects) Medical sciences Miscellaneous. Technology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Polymerase Chain Reaction - methods Salmonella typhi - isolation & purification Sensitivity and Specificity Tropical medicine Typhoid Fever - diagnosis
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