Native cellulose nanofibrills induce immune tolerance in vitro by acting on dendritic cells
Cellulose nanofibrills (CNFs) are attractive biocompatible, natural nanomaterials for wide biomedical applications. However, the immunological mechanisms of CNFs have been poorly investigated. Considering that dendritic cells (DCs) are the key immune regulatory cells in response to nanomaterials, ou...
Gespeichert in:
| Veröffentlicht in: | Scientific reports 2016-08, Vol.6 (1), p.31618, Article 31618 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 1 |
| container_start_page | 31618 |
| container_title | Scientific reports |
| container_volume | 6 |
| creator | Tomić, Sergej Kokol, Vanja Mihajlović, Dušan Mirčić, Aleksandar Čolić, Miodrag |
| description | Cellulose nanofibrills (CNFs) are attractive biocompatible, natural nanomaterials for wide biomedical applications. However, the immunological mechanisms of CNFs have been poorly investigated. Considering that dendritic cells (DCs) are the key immune regulatory cells in response to nanomaterials, our aim was to investigate the immunological mechanisms of CNFs in a model of DC-mediated immune response. We found that non-toxic concentrations of CNFs impaired the differentiation and subsequent maturation of human monocyte-derived (mo)-DCs. In a co-culture with CD4
+
T cells, CNF-treated mo-DCs possessed a weaker allostimulatory and T helper (Th)1 and Th17 polarizing capacity, but a stronger capacity to induce Th2 cells and CD4
+
CD25
hi
FoxP3
hi
regulatory T cells. This correlated with an increased immunoglobulin-like transcript-4 and indolamine dioxygenase-1 expression by CNF-treated mo-DCs, following the partial internalization of CNFs and the accumulation of CD209 and actin bundles at the place of contacts with CNFs. Cumulatively, we showed that CNFs are able to induce an active immune tolerance by inducing tolerogenic DCs, which could be beneficial for the application of CNFs in wound healing and chronic inflammation therapies. |
| doi_str_mv | 10.1038/srep31618 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4997350</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1898642176</sourcerecordid><originalsourceid>FETCH-LOGICAL-c438t-e64a8bc16648766b247a2a8ace0788259ade2bc3847e612960778343081f959b3</originalsourceid><addsrcrecordid>eNplkU1rGzEQhkVJqIOTQ_9AEPSUght9rT4ugRDStGCSS3vqQWi1Y1dhLTnSriH_PnKcGIfqIqF5eOZlBqEvlHynhOvLkmHNqaT6EzphRDQzxhk7OnhP0Fkpj6SehhlBzWc0YapptJLNCfp774awAeyh78c-FcDRxbQIbQ59X3CI3egBh9VqjICH1EN2cfsR8SYMOeH2GTs_hLjEKeIOYpfDEPyrrpyi44XrC5y93VP058ft75ufs_nD3a-b6_nMC66HGUjhdOuplKJmki0TyjGnnQeitGaNcR2w1nMtFEjKjCRKaS440XRhGtPyKbraeddju4LOQxyy6-06h5XLzza5YD9WYvhnl2ljhTGKN6QKvr4JcnoaoQz2MY051syWaqOlYFTJSl3sKJ9TqVNf7DtQYrersPtVVPb8MNKefB98Bb7tgFJLcQn5oOV_the4dZOU</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1898642176</pqid></control><display><type>article</type><title>Native cellulose nanofibrills induce immune tolerance in vitro by acting on dendritic cells</title><source>Nature Free</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature OA Free Journals</source><creator>Tomić, Sergej ; Kokol, Vanja ; Mihajlović, Dušan ; Mirčić, Aleksandar ; Čolić, Miodrag</creator><creatorcontrib>Tomić, Sergej ; Kokol, Vanja ; Mihajlović, Dušan ; Mirčić, Aleksandar ; Čolić, Miodrag</creatorcontrib><description>Cellulose nanofibrills (CNFs) are attractive biocompatible, natural nanomaterials for wide biomedical applications. However, the immunological mechanisms of CNFs have been poorly investigated. Considering that dendritic cells (DCs) are the key immune regulatory cells in response to nanomaterials, our aim was to investigate the immunological mechanisms of CNFs in a model of DC-mediated immune response. We found that non-toxic concentrations of CNFs impaired the differentiation and subsequent maturation of human monocyte-derived (mo)-DCs. In a co-culture with CD4
+
T cells, CNF-treated mo-DCs possessed a weaker allostimulatory and T helper (Th)1 and Th17 polarizing capacity, but a stronger capacity to induce Th2 cells and CD4
+
CD25
hi
FoxP3
hi
regulatory T cells. This correlated with an increased immunoglobulin-like transcript-4 and indolamine dioxygenase-1 expression by CNF-treated mo-DCs, following the partial internalization of CNFs and the accumulation of CD209 and actin bundles at the place of contacts with CNFs. Cumulatively, we showed that CNFs are able to induce an active immune tolerance by inducing tolerogenic DCs, which could be beneficial for the application of CNFs in wound healing and chronic inflammation therapies.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep31618</identifier><identifier>PMID: 27558765</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/1 ; 13/21 ; 13/51 ; 14/19 ; 14/28 ; 631/250/251/1574 ; 639/925/357/404 ; 96/106 ; 96/2 ; Actin ; CD25 antigen ; CD4 antigen ; Cell culture ; Cellulose ; Dendritic cells ; Dioxygenase ; Helper cells ; Humanities and Social Sciences ; Immune response ; Immunological tolerance ; Immunology ; Immunoregulation ; Internalization ; Lymphocytes T ; Monocytes ; multidisciplinary ; Nanomaterials ; Nanotechnology ; Science ; Transcription ; Wound healing</subject><ispartof>Scientific reports, 2016-08, Vol.6 (1), p.31618, Article 31618</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Nature Publishing Group Aug 2016</rights><rights>Copyright © 2016, The Author(s) 2016 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-e64a8bc16648766b247a2a8ace0788259ade2bc3847e612960778343081f959b3</citedby><cites>FETCH-LOGICAL-c438t-e64a8bc16648766b247a2a8ace0788259ade2bc3847e612960778343081f959b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997350/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4997350/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27558765$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tomić, Sergej</creatorcontrib><creatorcontrib>Kokol, Vanja</creatorcontrib><creatorcontrib>Mihajlović, Dušan</creatorcontrib><creatorcontrib>Mirčić, Aleksandar</creatorcontrib><creatorcontrib>Čolić, Miodrag</creatorcontrib><title>Native cellulose nanofibrills induce immune tolerance in vitro by acting on dendritic cells</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Cellulose nanofibrills (CNFs) are attractive biocompatible, natural nanomaterials for wide biomedical applications. However, the immunological mechanisms of CNFs have been poorly investigated. Considering that dendritic cells (DCs) are the key immune regulatory cells in response to nanomaterials, our aim was to investigate the immunological mechanisms of CNFs in a model of DC-mediated immune response. We found that non-toxic concentrations of CNFs impaired the differentiation and subsequent maturation of human monocyte-derived (mo)-DCs. In a co-culture with CD4
+
T cells, CNF-treated mo-DCs possessed a weaker allostimulatory and T helper (Th)1 and Th17 polarizing capacity, but a stronger capacity to induce Th2 cells and CD4
+
CD25
hi
FoxP3
hi
regulatory T cells. This correlated with an increased immunoglobulin-like transcript-4 and indolamine dioxygenase-1 expression by CNF-treated mo-DCs, following the partial internalization of CNFs and the accumulation of CD209 and actin bundles at the place of contacts with CNFs. Cumulatively, we showed that CNFs are able to induce an active immune tolerance by inducing tolerogenic DCs, which could be beneficial for the application of CNFs in wound healing and chronic inflammation therapies.</description><subject>13/1</subject><subject>13/21</subject><subject>13/51</subject><subject>14/19</subject><subject>14/28</subject><subject>631/250/251/1574</subject><subject>639/925/357/404</subject><subject>96/106</subject><subject>96/2</subject><subject>Actin</subject><subject>CD25 antigen</subject><subject>CD4 antigen</subject><subject>Cell culture</subject><subject>Cellulose</subject><subject>Dendritic cells</subject><subject>Dioxygenase</subject><subject>Helper cells</subject><subject>Humanities and Social Sciences</subject><subject>Immune response</subject><subject>Immunological tolerance</subject><subject>Immunology</subject><subject>Immunoregulation</subject><subject>Internalization</subject><subject>Lymphocytes T</subject><subject>Monocytes</subject><subject>multidisciplinary</subject><subject>Nanomaterials</subject><subject>Nanotechnology</subject><subject>Science</subject><subject>Transcription</subject><subject>Wound healing</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>BENPR</sourceid><recordid>eNplkU1rGzEQhkVJqIOTQ_9AEPSUght9rT4ugRDStGCSS3vqQWi1Y1dhLTnSriH_PnKcGIfqIqF5eOZlBqEvlHynhOvLkmHNqaT6EzphRDQzxhk7OnhP0Fkpj6SehhlBzWc0YapptJLNCfp774awAeyh78c-FcDRxbQIbQ59X3CI3egBh9VqjICH1EN2cfsR8SYMOeH2GTs_hLjEKeIOYpfDEPyrrpyi44XrC5y93VP058ft75ufs_nD3a-b6_nMC66HGUjhdOuplKJmki0TyjGnnQeitGaNcR2w1nMtFEjKjCRKaS440XRhGtPyKbraeddju4LOQxyy6-06h5XLzza5YD9WYvhnl2ljhTGKN6QKvr4JcnoaoQz2MY051syWaqOlYFTJSl3sKJ9TqVNf7DtQYrersPtVVPb8MNKefB98Bb7tgFJLcQn5oOV_the4dZOU</recordid><startdate>20160825</startdate><enddate>20160825</enddate><creator>Tomić, Sergej</creator><creator>Kokol, Vanja</creator><creator>Mihajlović, Dušan</creator><creator>Mirčić, Aleksandar</creator><creator>Čolić, Miodrag</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20160825</creationdate><title>Native cellulose nanofibrills induce immune tolerance in vitro by acting on dendritic cells</title><author>Tomić, Sergej ; Kokol, Vanja ; Mihajlović, Dušan ; Mirčić, Aleksandar ; Čolić, Miodrag</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-e64a8bc16648766b247a2a8ace0788259ade2bc3847e612960778343081f959b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>13/1</topic><topic>13/21</topic><topic>13/51</topic><topic>14/19</topic><topic>14/28</topic><topic>631/250/251/1574</topic><topic>639/925/357/404</topic><topic>96/106</topic><topic>96/2</topic><topic>Actin</topic><topic>CD25 antigen</topic><topic>CD4 antigen</topic><topic>Cell culture</topic><topic>Cellulose</topic><topic>Dendritic cells</topic><topic>Dioxygenase</topic><topic>Helper cells</topic><topic>Humanities and Social Sciences</topic><topic>Immune response</topic><topic>Immunological tolerance</topic><topic>Immunology</topic><topic>Immunoregulation</topic><topic>Internalization</topic><topic>Lymphocytes T</topic><topic>Monocytes</topic><topic>multidisciplinary</topic><topic>Nanomaterials</topic><topic>Nanotechnology</topic><topic>Science</topic><topic>Transcription</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tomić, Sergej</creatorcontrib><creatorcontrib>Kokol, Vanja</creatorcontrib><creatorcontrib>Mihajlović, Dušan</creatorcontrib><creatorcontrib>Mirčić, Aleksandar</creatorcontrib><creatorcontrib>Čolić, Miodrag</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tomić, Sergej</au><au>Kokol, Vanja</au><au>Mihajlović, Dušan</au><au>Mirčić, Aleksandar</au><au>Čolić, Miodrag</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Native cellulose nanofibrills induce immune tolerance in vitro by acting on dendritic cells</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-08-25</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>31618</spage><pages>31618-</pages><artnum>31618</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Cellulose nanofibrills (CNFs) are attractive biocompatible, natural nanomaterials for wide biomedical applications. However, the immunological mechanisms of CNFs have been poorly investigated. Considering that dendritic cells (DCs) are the key immune regulatory cells in response to nanomaterials, our aim was to investigate the immunological mechanisms of CNFs in a model of DC-mediated immune response. We found that non-toxic concentrations of CNFs impaired the differentiation and subsequent maturation of human monocyte-derived (mo)-DCs. In a co-culture with CD4
+
T cells, CNF-treated mo-DCs possessed a weaker allostimulatory and T helper (Th)1 and Th17 polarizing capacity, but a stronger capacity to induce Th2 cells and CD4
+
CD25
hi
FoxP3
hi
regulatory T cells. This correlated with an increased immunoglobulin-like transcript-4 and indolamine dioxygenase-1 expression by CNF-treated mo-DCs, following the partial internalization of CNFs and the accumulation of CD209 and actin bundles at the place of contacts with CNFs. Cumulatively, we showed that CNFs are able to induce an active immune tolerance by inducing tolerogenic DCs, which could be beneficial for the application of CNFs in wound healing and chronic inflammation therapies.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27558765</pmid><doi>10.1038/srep31618</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2016-08, Vol.6 (1), p.31618, Article 31618 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4997350 |
| source | Nature Free; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; Springer Nature OA Free Journals |
| subjects | 13/1 13/21 13/51 14/19 14/28 631/250/251/1574 639/925/357/404 96/106 96/2 Actin CD25 antigen CD4 antigen Cell culture Cellulose Dendritic cells Dioxygenase Helper cells Humanities and Social Sciences Immune response Immunological tolerance Immunology Immunoregulation Internalization Lymphocytes T Monocytes multidisciplinary Nanomaterials Nanotechnology Science Transcription Wound healing |
| title | Native cellulose nanofibrills induce immune tolerance in vitro by acting on dendritic cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T08%3A12%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Native%20cellulose%20nanofibrills%20induce%20immune%20tolerance%20in%20vitro%20by%20acting%20on%20dendritic%20cells&rft.jtitle=Scientific%20reports&rft.au=Tomi%C4%87,%20Sergej&rft.date=2016-08-25&rft.volume=6&rft.issue=1&rft.spage=31618&rft.pages=31618-&rft.artnum=31618&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/srep31618&rft_dat=%3Cproquest_pubme%3E1898642176%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1898642176&rft_id=info:pmid/27558765&rfr_iscdi=true |