Nitrogen fixation and nifH diversity in human gut microbiota
It has been hypothesized that nitrogen fixation occurs in the human gut. However, whether the gut microbiota truly has this potential remains unclear. We investigated the nitrogen-fixing activity and diversity of the nitrogenase reductase (NifH) genes in the faecal microbiota of humans, focusing on...
Gespeichert in:
| Veröffentlicht in: | Scientific reports 2016-08, Vol.6 (1), p.31942-31942, Article 31942 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 31942 |
|---|---|
| container_issue | 1 |
| container_start_page | 31942 |
| container_title | Scientific reports |
| container_volume | 6 |
| creator | Igai, Katsura Itakura, Manabu Nishijima, Suguru Tsurumaru, Hirohito Suda, Wataru Tsutaya, Takumi Tomitsuka, Eriko Tadokoro, Kiyoshi Baba, Jun Odani, Shingo Natsuhara, Kazumi Morita, Ayako Yoneda, Minoru Greenhill, Andrew R. Horwood, Paul F. Inoue, Jun-ichi Ohkuma, Moriya Hongoh, Yuichi Yamamoto, Taro Siba, Peter M. Hattori, Masahira Minamisawa, Kiwamu Umezaki, Masahiro |
| description | It has been hypothesized that nitrogen fixation occurs in the human gut. However, whether the gut microbiota truly has this potential remains unclear. We investigated the nitrogen-fixing activity and diversity of the nitrogenase reductase (NifH) genes in the faecal microbiota of humans, focusing on Papua New Guinean and Japanese individuals with low to high habitual nitrogen intake. A
15
N
2
incorporation assay showed significant enrichment of
15
N in all faecal samples, irrespective of the host nitrogen intake, which was also supported by an acetylene reduction assay. The fixed nitrogen corresponded to 0.01% of the standard nitrogen requirement for humans, although our data implied that the contribution in the gut
in vivo
might be higher than this value. The
nifH
genes recovered in cloning and metagenomic analyses were classified in two clusters: one comprising sequences almost identical to
Klebsiella
sequences and the other related to sequences of Clostridiales members. These results are consistent with an analysis of databases of faecal metagenomes from other human populations. Collectively, the human gut microbiota has a potential for nitrogen fixation, which may be attributable to
Klebsiella
and Clostridiales strains, although no evidence was found that the nitrogen-fixing activity substantially contributes to the host nitrogen balance. |
| doi_str_mv | 10.1038/srep31942 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4995403</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1898642179</sourcerecordid><originalsourceid>FETCH-LOGICAL-c632t-fc86b6d5fb9e2896d61c69ed5497aea413e35d67040cbd617eb4d95935a32143</originalsourceid><addsrcrecordid>eNplkV1LwzAYhYMoTuYu_ANS8EaFavPZBkSQoU4YerP7kLZpl9EmM2mH-_dmbI6puXkD5-G8HweAC5jcwQRn996pJYacoCNwhhJCY4QROj74D8DI-0USHkWcQH4KBiillGBCzsDDu-6crZWJKv0lO21NJE0ZGV1NolKvlPO6W0faRPO-lSaq-y5qdeFsrm0nz8FJJRuvRrs6BLOX59l4Ek8_Xt_GT9O4YBh1cVVkLGclrXKuUMZZyWDBuCop4alUkkCsMC1ZmpCkyIOYqpyUnHJMJUaQ4CF43Nou-7xVZaFM52Qjlk630q2FlVr8Voyei9quBOGckgQHg-udgbOfvfKdaLUvVNNIo2zvBcwgCX1SwgN69Qdd2N6ZsF2geMYIgumGutlS4RI-BFDth4GJ2KQi9qkE9vJw-j35k0EAbreAD5KplTto-c_tG_v-lgU</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1898642179</pqid></control><display><type>article</type><title>Nitrogen fixation and nifH diversity in human gut microbiota</title><source>MEDLINE</source><source>Nature Free</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature OA Free Journals</source><creator>Igai, Katsura ; Itakura, Manabu ; Nishijima, Suguru ; Tsurumaru, Hirohito ; Suda, Wataru ; Tsutaya, Takumi ; Tomitsuka, Eriko ; Tadokoro, Kiyoshi ; Baba, Jun ; Odani, Shingo ; Natsuhara, Kazumi ; Morita, Ayako ; Yoneda, Minoru ; Greenhill, Andrew R. ; Horwood, Paul F. ; Inoue, Jun-ichi ; Ohkuma, Moriya ; Hongoh, Yuichi ; Yamamoto, Taro ; Siba, Peter M. ; Hattori, Masahira ; Minamisawa, Kiwamu ; Umezaki, Masahiro</creator><creatorcontrib>Igai, Katsura ; Itakura, Manabu ; Nishijima, Suguru ; Tsurumaru, Hirohito ; Suda, Wataru ; Tsutaya, Takumi ; Tomitsuka, Eriko ; Tadokoro, Kiyoshi ; Baba, Jun ; Odani, Shingo ; Natsuhara, Kazumi ; Morita, Ayako ; Yoneda, Minoru ; Greenhill, Andrew R. ; Horwood, Paul F. ; Inoue, Jun-ichi ; Ohkuma, Moriya ; Hongoh, Yuichi ; Yamamoto, Taro ; Siba, Peter M. ; Hattori, Masahira ; Minamisawa, Kiwamu ; Umezaki, Masahiro</creatorcontrib><description>It has been hypothesized that nitrogen fixation occurs in the human gut. However, whether the gut microbiota truly has this potential remains unclear. We investigated the nitrogen-fixing activity and diversity of the nitrogenase reductase (NifH) genes in the faecal microbiota of humans, focusing on Papua New Guinean and Japanese individuals with low to high habitual nitrogen intake. A
15
N
2
incorporation assay showed significant enrichment of
15
N in all faecal samples, irrespective of the host nitrogen intake, which was also supported by an acetylene reduction assay. The fixed nitrogen corresponded to 0.01% of the standard nitrogen requirement for humans, although our data implied that the contribution in the gut
in vivo
might be higher than this value. The
nifH
genes recovered in cloning and metagenomic analyses were classified in two clusters: one comprising sequences almost identical to
Klebsiella
sequences and the other related to sequences of Clostridiales members. These results are consistent with an analysis of databases of faecal metagenomes from other human populations. Collectively, the human gut microbiota has a potential for nitrogen fixation, which may be attributable to
Klebsiella
and Clostridiales strains, although no evidence was found that the nitrogen-fixing activity substantially contributes to the host nitrogen balance.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep31942</identifier><identifier>PMID: 27554344</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>45/23 ; 45/77 ; 45/90 ; 631/326/171 ; 631/326/2565/855 ; Acetylene ; Acetylene - chemistry ; Acetylene - metabolism ; Acetylene reduction ; Adult ; Bacterial Proteins - classification ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Cloning ; Clostridiales - enzymology ; Clostridiales - genetics ; Clostridiales - isolation & purification ; Databases, Factual ; Feces - microbiology ; Female ; Gastrointestinal Microbiome ; Human populations ; Humanities and Social Sciences ; Humans ; Intestinal microflora ; Klebsiella - enzymology ; Klebsiella - genetics ; Klebsiella - isolation & purification ; Male ; Metagenomics ; multidisciplinary ; NifH gene ; Nitrogen ; Nitrogen - chemistry ; Nitrogen - metabolism ; Nitrogen balance ; Nitrogen Fixation ; Nitrogen Isotopes - metabolism ; Nitrogenase ; Nitrogenase reductase ; Oxidoreductases - classification ; Oxidoreductases - genetics ; Oxidoreductases - metabolism ; Phylogeny ; RNA, Bacterial - chemistry ; RNA, Bacterial - isolation & purification ; RNA, Bacterial - metabolism ; Science ; Science (multidisciplinary) ; Sequence Analysis, DNA ; Young Adult</subject><ispartof>Scientific reports, 2016-08, Vol.6 (1), p.31942-31942, Article 31942</ispartof><rights>The Author(s) 2016</rights><rights>Copyright Nature Publishing Group Aug 2016</rights><rights>Copyright © 2016, The Author(s) 2016 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c632t-fc86b6d5fb9e2896d61c69ed5497aea413e35d67040cbd617eb4d95935a32143</citedby><cites>FETCH-LOGICAL-c632t-fc86b6d5fb9e2896d61c69ed5497aea413e35d67040cbd617eb4d95935a32143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4995403/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4995403/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27554344$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Igai, Katsura</creatorcontrib><creatorcontrib>Itakura, Manabu</creatorcontrib><creatorcontrib>Nishijima, Suguru</creatorcontrib><creatorcontrib>Tsurumaru, Hirohito</creatorcontrib><creatorcontrib>Suda, Wataru</creatorcontrib><creatorcontrib>Tsutaya, Takumi</creatorcontrib><creatorcontrib>Tomitsuka, Eriko</creatorcontrib><creatorcontrib>Tadokoro, Kiyoshi</creatorcontrib><creatorcontrib>Baba, Jun</creatorcontrib><creatorcontrib>Odani, Shingo</creatorcontrib><creatorcontrib>Natsuhara, Kazumi</creatorcontrib><creatorcontrib>Morita, Ayako</creatorcontrib><creatorcontrib>Yoneda, Minoru</creatorcontrib><creatorcontrib>Greenhill, Andrew R.</creatorcontrib><creatorcontrib>Horwood, Paul F.</creatorcontrib><creatorcontrib>Inoue, Jun-ichi</creatorcontrib><creatorcontrib>Ohkuma, Moriya</creatorcontrib><creatorcontrib>Hongoh, Yuichi</creatorcontrib><creatorcontrib>Yamamoto, Taro</creatorcontrib><creatorcontrib>Siba, Peter M.</creatorcontrib><creatorcontrib>Hattori, Masahira</creatorcontrib><creatorcontrib>Minamisawa, Kiwamu</creatorcontrib><creatorcontrib>Umezaki, Masahiro</creatorcontrib><title>Nitrogen fixation and nifH diversity in human gut microbiota</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>It has been hypothesized that nitrogen fixation occurs in the human gut. However, whether the gut microbiota truly has this potential remains unclear. We investigated the nitrogen-fixing activity and diversity of the nitrogenase reductase (NifH) genes in the faecal microbiota of humans, focusing on Papua New Guinean and Japanese individuals with low to high habitual nitrogen intake. A
15
N
2
incorporation assay showed significant enrichment of
15
N in all faecal samples, irrespective of the host nitrogen intake, which was also supported by an acetylene reduction assay. The fixed nitrogen corresponded to 0.01% of the standard nitrogen requirement for humans, although our data implied that the contribution in the gut
in vivo
might be higher than this value. The
nifH
genes recovered in cloning and metagenomic analyses were classified in two clusters: one comprising sequences almost identical to
Klebsiella
sequences and the other related to sequences of Clostridiales members. These results are consistent with an analysis of databases of faecal metagenomes from other human populations. Collectively, the human gut microbiota has a potential for nitrogen fixation, which may be attributable to
Klebsiella
and Clostridiales strains, although no evidence was found that the nitrogen-fixing activity substantially contributes to the host nitrogen balance.</description><subject>45/23</subject><subject>45/77</subject><subject>45/90</subject><subject>631/326/171</subject><subject>631/326/2565/855</subject><subject>Acetylene</subject><subject>Acetylene - chemistry</subject><subject>Acetylene - metabolism</subject><subject>Acetylene reduction</subject><subject>Adult</subject><subject>Bacterial Proteins - classification</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Cloning</subject><subject>Clostridiales - enzymology</subject><subject>Clostridiales - genetics</subject><subject>Clostridiales - isolation & purification</subject><subject>Databases, Factual</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Gastrointestinal Microbiome</subject><subject>Human populations</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Intestinal microflora</subject><subject>Klebsiella - enzymology</subject><subject>Klebsiella - genetics</subject><subject>Klebsiella - isolation & purification</subject><subject>Male</subject><subject>Metagenomics</subject><subject>multidisciplinary</subject><subject>NifH gene</subject><subject>Nitrogen</subject><subject>Nitrogen - chemistry</subject><subject>Nitrogen - metabolism</subject><subject>Nitrogen balance</subject><subject>Nitrogen Fixation</subject><subject>Nitrogen Isotopes - metabolism</subject><subject>Nitrogenase</subject><subject>Nitrogenase reductase</subject><subject>Oxidoreductases - classification</subject><subject>Oxidoreductases - genetics</subject><subject>Oxidoreductases - metabolism</subject><subject>Phylogeny</subject><subject>RNA, Bacterial - chemistry</subject><subject>RNA, Bacterial - isolation & purification</subject><subject>RNA, Bacterial - metabolism</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Sequence Analysis, DNA</subject><subject>Young Adult</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNplkV1LwzAYhYMoTuYu_ANS8EaFavPZBkSQoU4YerP7kLZpl9EmM2mH-_dmbI6puXkD5-G8HweAC5jcwQRn996pJYacoCNwhhJCY4QROj74D8DI-0USHkWcQH4KBiillGBCzsDDu-6crZWJKv0lO21NJE0ZGV1NolKvlPO6W0faRPO-lSaq-y5qdeFsrm0nz8FJJRuvRrs6BLOX59l4Ek8_Xt_GT9O4YBh1cVVkLGclrXKuUMZZyWDBuCop4alUkkCsMC1ZmpCkyIOYqpyUnHJMJUaQ4CF43Nou-7xVZaFM52Qjlk630q2FlVr8Voyei9quBOGckgQHg-udgbOfvfKdaLUvVNNIo2zvBcwgCX1SwgN69Qdd2N6ZsF2geMYIgumGutlS4RI-BFDth4GJ2KQi9qkE9vJw-j35k0EAbreAD5KplTto-c_tG_v-lgU</recordid><startdate>20160824</startdate><enddate>20160824</enddate><creator>Igai, Katsura</creator><creator>Itakura, Manabu</creator><creator>Nishijima, Suguru</creator><creator>Tsurumaru, Hirohito</creator><creator>Suda, Wataru</creator><creator>Tsutaya, Takumi</creator><creator>Tomitsuka, Eriko</creator><creator>Tadokoro, Kiyoshi</creator><creator>Baba, Jun</creator><creator>Odani, Shingo</creator><creator>Natsuhara, Kazumi</creator><creator>Morita, Ayako</creator><creator>Yoneda, Minoru</creator><creator>Greenhill, Andrew R.</creator><creator>Horwood, Paul F.</creator><creator>Inoue, Jun-ichi</creator><creator>Ohkuma, Moriya</creator><creator>Hongoh, Yuichi</creator><creator>Yamamoto, Taro</creator><creator>Siba, Peter M.</creator><creator>Hattori, Masahira</creator><creator>Minamisawa, Kiwamu</creator><creator>Umezaki, Masahiro</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160824</creationdate><title>Nitrogen fixation and nifH diversity in human gut microbiota</title><author>Igai, Katsura ; Itakura, Manabu ; Nishijima, Suguru ; Tsurumaru, Hirohito ; Suda, Wataru ; Tsutaya, Takumi ; Tomitsuka, Eriko ; Tadokoro, Kiyoshi ; Baba, Jun ; Odani, Shingo ; Natsuhara, Kazumi ; Morita, Ayako ; Yoneda, Minoru ; Greenhill, Andrew R. ; Horwood, Paul F. ; Inoue, Jun-ichi ; Ohkuma, Moriya ; Hongoh, Yuichi ; Yamamoto, Taro ; Siba, Peter M. ; Hattori, Masahira ; Minamisawa, Kiwamu ; Umezaki, Masahiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c632t-fc86b6d5fb9e2896d61c69ed5497aea413e35d67040cbd617eb4d95935a32143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>45/23</topic><topic>45/77</topic><topic>45/90</topic><topic>631/326/171</topic><topic>631/326/2565/855</topic><topic>Acetylene</topic><topic>Acetylene - chemistry</topic><topic>Acetylene - metabolism</topic><topic>Acetylene reduction</topic><topic>Adult</topic><topic>Bacterial Proteins - classification</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Cloning</topic><topic>Clostridiales - enzymology</topic><topic>Clostridiales - genetics</topic><topic>Clostridiales - isolation & purification</topic><topic>Databases, Factual</topic><topic>Feces - microbiology</topic><topic>Female</topic><topic>Gastrointestinal Microbiome</topic><topic>Human populations</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Intestinal microflora</topic><topic>Klebsiella - enzymology</topic><topic>Klebsiella - genetics</topic><topic>Klebsiella - isolation & purification</topic><topic>Male</topic><topic>Metagenomics</topic><topic>multidisciplinary</topic><topic>NifH gene</topic><topic>Nitrogen</topic><topic>Nitrogen - chemistry</topic><topic>Nitrogen - metabolism</topic><topic>Nitrogen balance</topic><topic>Nitrogen Fixation</topic><topic>Nitrogen Isotopes - metabolism</topic><topic>Nitrogenase</topic><topic>Nitrogenase reductase</topic><topic>Oxidoreductases - classification</topic><topic>Oxidoreductases - genetics</topic><topic>Oxidoreductases - metabolism</topic><topic>Phylogeny</topic><topic>RNA, Bacterial - chemistry</topic><topic>RNA, Bacterial - isolation & purification</topic><topic>RNA, Bacterial - metabolism</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Sequence Analysis, DNA</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Igai, Katsura</creatorcontrib><creatorcontrib>Itakura, Manabu</creatorcontrib><creatorcontrib>Nishijima, Suguru</creatorcontrib><creatorcontrib>Tsurumaru, Hirohito</creatorcontrib><creatorcontrib>Suda, Wataru</creatorcontrib><creatorcontrib>Tsutaya, Takumi</creatorcontrib><creatorcontrib>Tomitsuka, Eriko</creatorcontrib><creatorcontrib>Tadokoro, Kiyoshi</creatorcontrib><creatorcontrib>Baba, Jun</creatorcontrib><creatorcontrib>Odani, Shingo</creatorcontrib><creatorcontrib>Natsuhara, Kazumi</creatorcontrib><creatorcontrib>Morita, Ayako</creatorcontrib><creatorcontrib>Yoneda, Minoru</creatorcontrib><creatorcontrib>Greenhill, Andrew R.</creatorcontrib><creatorcontrib>Horwood, Paul F.</creatorcontrib><creatorcontrib>Inoue, Jun-ichi</creatorcontrib><creatorcontrib>Ohkuma, Moriya</creatorcontrib><creatorcontrib>Hongoh, Yuichi</creatorcontrib><creatorcontrib>Yamamoto, Taro</creatorcontrib><creatorcontrib>Siba, Peter M.</creatorcontrib><creatorcontrib>Hattori, Masahira</creatorcontrib><creatorcontrib>Minamisawa, Kiwamu</creatorcontrib><creatorcontrib>Umezaki, Masahiro</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Igai, Katsura</au><au>Itakura, Manabu</au><au>Nishijima, Suguru</au><au>Tsurumaru, Hirohito</au><au>Suda, Wataru</au><au>Tsutaya, Takumi</au><au>Tomitsuka, Eriko</au><au>Tadokoro, Kiyoshi</au><au>Baba, Jun</au><au>Odani, Shingo</au><au>Natsuhara, Kazumi</au><au>Morita, Ayako</au><au>Yoneda, Minoru</au><au>Greenhill, Andrew R.</au><au>Horwood, Paul F.</au><au>Inoue, Jun-ichi</au><au>Ohkuma, Moriya</au><au>Hongoh, Yuichi</au><au>Yamamoto, Taro</au><au>Siba, Peter M.</au><au>Hattori, Masahira</au><au>Minamisawa, Kiwamu</au><au>Umezaki, Masahiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitrogen fixation and nifH diversity in human gut microbiota</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2016-08-24</date><risdate>2016</risdate><volume>6</volume><issue>1</issue><spage>31942</spage><epage>31942</epage><pages>31942-31942</pages><artnum>31942</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>It has been hypothesized that nitrogen fixation occurs in the human gut. However, whether the gut microbiota truly has this potential remains unclear. We investigated the nitrogen-fixing activity and diversity of the nitrogenase reductase (NifH) genes in the faecal microbiota of humans, focusing on Papua New Guinean and Japanese individuals with low to high habitual nitrogen intake. A
15
N
2
incorporation assay showed significant enrichment of
15
N in all faecal samples, irrespective of the host nitrogen intake, which was also supported by an acetylene reduction assay. The fixed nitrogen corresponded to 0.01% of the standard nitrogen requirement for humans, although our data implied that the contribution in the gut
in vivo
might be higher than this value. The
nifH
genes recovered in cloning and metagenomic analyses were classified in two clusters: one comprising sequences almost identical to
Klebsiella
sequences and the other related to sequences of Clostridiales members. These results are consistent with an analysis of databases of faecal metagenomes from other human populations. Collectively, the human gut microbiota has a potential for nitrogen fixation, which may be attributable to
Klebsiella
and Clostridiales strains, although no evidence was found that the nitrogen-fixing activity substantially contributes to the host nitrogen balance.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>27554344</pmid><doi>10.1038/srep31942</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2016-08, Vol.6 (1), p.31942-31942, Article 31942 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4995403 |
| source | MEDLINE; Nature Free; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; Springer Nature OA Free Journals |
| subjects | 45/23 45/77 45/90 631/326/171 631/326/2565/855 Acetylene Acetylene - chemistry Acetylene - metabolism Acetylene reduction Adult Bacterial Proteins - classification Bacterial Proteins - genetics Bacterial Proteins - metabolism Cloning Clostridiales - enzymology Clostridiales - genetics Clostridiales - isolation & purification Databases, Factual Feces - microbiology Female Gastrointestinal Microbiome Human populations Humanities and Social Sciences Humans Intestinal microflora Klebsiella - enzymology Klebsiella - genetics Klebsiella - isolation & purification Male Metagenomics multidisciplinary NifH gene Nitrogen Nitrogen - chemistry Nitrogen - metabolism Nitrogen balance Nitrogen Fixation Nitrogen Isotopes - metabolism Nitrogenase Nitrogenase reductase Oxidoreductases - classification Oxidoreductases - genetics Oxidoreductases - metabolism Phylogeny RNA, Bacterial - chemistry RNA, Bacterial - isolation & purification RNA, Bacterial - metabolism Science Science (multidisciplinary) Sequence Analysis, DNA Young Adult |
| title | Nitrogen fixation and nifH diversity in human gut microbiota |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T06%3A38%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nitrogen%20fixation%20and%20nifH%20diversity%20in%20human%20gut%20microbiota&rft.jtitle=Scientific%20reports&rft.au=Igai,%20Katsura&rft.date=2016-08-24&rft.volume=6&rft.issue=1&rft.spage=31942&rft.epage=31942&rft.pages=31942-31942&rft.artnum=31942&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/srep31942&rft_dat=%3Cproquest_pubme%3E1898642179%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1898642179&rft_id=info:pmid/27554344&rfr_iscdi=true |