CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice

Targeted gene disrupted mice can be efficiently generated by expressing a single guide RNA (sgRNA)/CAS9 complex in the zygote. However, the limited success of complicated genome editing, such as large deletions, point mutations, and knockins, remains to be improved. Further, the mosaicism in founder...

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Veröffentlicht in:	Scientific reports 2016-08, Vol.6 (1), p.31666, Article 31666
Hauptverfasser:	Oji, Asami, Noda, Taichi, Fujihara, Yoshitaka, Miyata, Haruhiko, Kim, Yeon Joo, Muto, Masanaga, Nozawa, Kaori, Matsumura, Takafumi, Isotani, Ayako, Ikawa, Masahito
Format:	Artikel
Sprache:	eng
Schlagworte:	38/109 631/1647 631/61 96/100 Animals Apoptosis Regulatory Proteins Chimera - genetics Cilia CRISPR CRISPR-Cas Systems Editing Embryo cells Embryonic Stem Cells - metabolism Female Gene Deletion Gene Editing - methods Gene Knock-In Techniques Genes, Lethal Genome editing Genomes HSP40 Heat-Shock Proteins - genetics Humanities and Social Sciences Hydrocephalus Hydrocephalus - genetics Hydrocephalus - pathology INDEL Mutation Male Mice Mice, Inbred C57BL Mice, Inbred DBA Mice, Inbred ICR Mice, Transgenic Molecular Chaperones Mosaicism multidisciplinary Mutation Point Mutation Pregnancy Ribonucleic acid RNA RNA, Guide, CRISPR-Cas Systems - genetics Science Science (multidisciplinary) Sperm Motility - genetics Spermatozoa - abnormalities Stem cell transplantation Stem cells
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