Anti-methicillin Resistant Staphylococcus aureus Compound Isolation from Halophilic Bacillus amyloliquefaciens MHB1 and Determination of Its Mode of Action Using Electron Microscope and Flow Cytometry Analysis
The aim of this study was to purify, characterize and evaluate the antibacterial activity of bioactive compound against methicillin-resistant Staphylococcus aureus (MRSA). The anti-MRSA compound was produced by a halophilic bacterial strain designated as MHB1. The MHB1 strain exhibited 99 % similari...
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| Veröffentlicht in: | Indian journal of microbiology 2016-06, Vol.56 (2), p.148-157 |
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| creator | Jeyanthi, Venkadapathi Velusamy, Palaniyandi |
| description | The aim of this study was to purify, characterize and evaluate the antibacterial activity of bioactive compound against methicillin-resistant
Staphylococcus aureus
(MRSA). The anti-MRSA compound was produced by a halophilic bacterial strain designated as MHB1. The MHB1 strain exhibited 99 % similarity to
Bacillus amyloliquefaciens
based on 16S rRNA gene analysis. The culture conditions of
Bacillus amyloliquefaciens
MHB1 were optimized using nutritional and environmental parameters for enhanced anti-MRSA compound production. The pure bioactive compound was isolated using silica gel column chromatography and Semi-preparative High-performance liquid chromatography (Semi-preparative HPLC). The Thin layer chromatography, Fourier transform infrared spectroscopy and proton NMR (
1
H NMR) analysis indicated the phenolic nature of the compound. The molecular mass of the purified compound was 507 Da as revealed by Liquid chromatography-mass spectrometry (LC–MS) analysis. The compound inhibited the growth of MRSA with minimum inhibitory concentration (MIC) of 62.5 µg mL
−1
. MRSA bacteria exposed to 4× MIC of the compound and the cell viability was determined using flow cytometric analysis. Scanning electron microscope and Transmission electron microscope analysis was used to determine the ultrastructural changes in bacteria. This is the first report on isolation of anti-MRSA compound from halophilic
B. amyloliquefaciens
MHB1 and could act as a promising biocontrol agent. |
| doi_str_mv | 10.1007/s12088-016-0566-8 |
| format | Article |
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Staphylococcus aureus
(MRSA). The anti-MRSA compound was produced by a halophilic bacterial strain designated as MHB1. The MHB1 strain exhibited 99 % similarity to
Bacillus amyloliquefaciens
based on 16S rRNA gene analysis. The culture conditions of
Bacillus amyloliquefaciens
MHB1 were optimized using nutritional and environmental parameters for enhanced anti-MRSA compound production. The pure bioactive compound was isolated using silica gel column chromatography and Semi-preparative High-performance liquid chromatography (Semi-preparative HPLC). The Thin layer chromatography, Fourier transform infrared spectroscopy and proton NMR (
1
H NMR) analysis indicated the phenolic nature of the compound. The molecular mass of the purified compound was 507 Da as revealed by Liquid chromatography-mass spectrometry (LC–MS) analysis. The compound inhibited the growth of MRSA with minimum inhibitory concentration (MIC) of 62.5 µg mL
−1
. MRSA bacteria exposed to 4× MIC of the compound and the cell viability was determined using flow cytometric analysis. Scanning electron microscope and Transmission electron microscope analysis was used to determine the ultrastructural changes in bacteria. This is the first report on isolation of anti-MRSA compound from halophilic
B. amyloliquefaciens
MHB1 and could act as a promising biocontrol agent.</description><identifier>ISSN: 0046-8991</identifier><identifier>EISSN: 0973-7715</identifier><identifier>DOI: 10.1007/s12088-016-0566-8</identifier><identifier>PMID: 27570306</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>antibacterial properties ; antibiotic resistance ; Bacillus amyloliquefaciens ; Bacteria ; Bioactive compounds ; Biological control ; biological control agents ; Biomedical and Life Sciences ; cell viability ; Chromatography ; Electron microscopes ; environmental factors ; Flow cytometry ; Fourier transform infrared spectroscopy ; Fourier transforms ; genes ; growth retardation ; high performance liquid chromatography ; Infrared spectroscopy ; Laboratories ; Life Sciences ; Liquid chromatography ; Mass spectrometry ; mechanism of action ; Medical Microbiology ; Metabolites ; methicillin ; Microbiology ; minimum inhibitory concentration ; Mode of action ; molecular weight ; nuclear magnetic resonance spectroscopy ; Original ; Original Article ; Pathogens ; Phenols ; ribosomal RNA ; Salinity ; scanning electron microscopes ; Silica ; silica gel ; Staphylococcus aureus ; Staphylococcus infections ; Thin layer chromatography ; transmission electron microscopes</subject><ispartof>Indian journal of microbiology, 2016-06, Vol.56 (2), p.148-157</ispartof><rights>Association of Microbiologists of India 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-c69d3d62b754962d18dcb55a2757d5be4adc7dd9174205780573bd0d6dd38f823</citedby><cites>FETCH-LOGICAL-c536t-c69d3d62b754962d18dcb55a2757d5be4adc7dd9174205780573bd0d6dd38f823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4984441/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4984441/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,725,778,782,883,27911,27912,41475,42544,51306,53778,53780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27570306$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jeyanthi, Venkadapathi</creatorcontrib><creatorcontrib>Velusamy, Palaniyandi</creatorcontrib><title>Anti-methicillin Resistant Staphylococcus aureus Compound Isolation from Halophilic Bacillus amyloliquefaciens MHB1 and Determination of Its Mode of Action Using Electron Microscope and Flow Cytometry Analysis</title><title>Indian journal of microbiology</title><addtitle>Indian J Microbiol</addtitle><addtitle>Indian J Microbiol</addtitle><description>The aim of this study was to purify, characterize and evaluate the antibacterial activity of bioactive compound against methicillin-resistant
Staphylococcus aureus
(MRSA). The anti-MRSA compound was produced by a halophilic bacterial strain designated as MHB1. The MHB1 strain exhibited 99 % similarity to
Bacillus amyloliquefaciens
based on 16S rRNA gene analysis. The culture conditions of
Bacillus amyloliquefaciens
MHB1 were optimized using nutritional and environmental parameters for enhanced anti-MRSA compound production. The pure bioactive compound was isolated using silica gel column chromatography and Semi-preparative High-performance liquid chromatography (Semi-preparative HPLC). The Thin layer chromatography, Fourier transform infrared spectroscopy and proton NMR (
1
H NMR) analysis indicated the phenolic nature of the compound. The molecular mass of the purified compound was 507 Da as revealed by Liquid chromatography-mass spectrometry (LC–MS) analysis. The compound inhibited the growth of MRSA with minimum inhibitory concentration (MIC) of 62.5 µg mL
−1
. MRSA bacteria exposed to 4× MIC of the compound and the cell viability was determined using flow cytometric analysis. Scanning electron microscope and Transmission electron microscope analysis was used to determine the ultrastructural changes in bacteria. This is the first report on isolation of anti-MRSA compound from halophilic
B. amyloliquefaciens
MHB1 and could act as a promising biocontrol agent.</description><subject>antibacterial properties</subject><subject>antibiotic resistance</subject><subject>Bacillus amyloliquefaciens</subject><subject>Bacteria</subject><subject>Bioactive compounds</subject><subject>Biological control</subject><subject>biological control agents</subject><subject>Biomedical and Life Sciences</subject><subject>cell viability</subject><subject>Chromatography</subject><subject>Electron microscopes</subject><subject>environmental factors</subject><subject>Flow cytometry</subject><subject>Fourier transform infrared spectroscopy</subject><subject>Fourier transforms</subject><subject>genes</subject><subject>growth retardation</subject><subject>high performance liquid chromatography</subject><subject>Infrared spectroscopy</subject><subject>Laboratories</subject><subject>Life Sciences</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>mechanism of action</subject><subject>Medical Microbiology</subject><subject>Metabolites</subject><subject>methicillin</subject><subject>Microbiology</subject><subject>minimum inhibitory concentration</subject><subject>Mode of action</subject><subject>molecular weight</subject><subject>nuclear magnetic resonance spectroscopy</subject><subject>Original</subject><subject>Original Article</subject><subject>Pathogens</subject><subject>Phenols</subject><subject>ribosomal RNA</subject><subject>Salinity</subject><subject>scanning electron microscopes</subject><subject>Silica</subject><subject>silica gel</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus infections</subject><subject>Thin layer chromatography</subject><subject>transmission electron 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Venkadapathi</creator><creator>Velusamy, Palaniyandi</creator><general>Springer India</general><general>Springer Nature 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amyloliquefaciens MHB1 and Determination of Its Mode of Action Using Electron Microscope and Flow Cytometry Analysis</title><author>Jeyanthi, Venkadapathi ; Velusamy, Palaniyandi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c536t-c69d3d62b754962d18dcb55a2757d5be4adc7dd9174205780573bd0d6dd38f823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>antibacterial properties</topic><topic>antibiotic resistance</topic><topic>Bacillus amyloliquefaciens</topic><topic>Bacteria</topic><topic>Bioactive compounds</topic><topic>Biological control</topic><topic>biological control agents</topic><topic>Biomedical and Life Sciences</topic><topic>cell viability</topic><topic>Chromatography</topic><topic>Electron microscopes</topic><topic>environmental factors</topic><topic>Flow cytometry</topic><topic>Fourier transform infrared spectroscopy</topic><topic>Fourier 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Isolation from Halophilic Bacillus amyloliquefaciens MHB1 and Determination of Its Mode of Action Using Electron Microscope and Flow Cytometry Analysis</atitle><jtitle>Indian journal of microbiology</jtitle><stitle>Indian J Microbiol</stitle><addtitle>Indian J Microbiol</addtitle><date>2016-06-01</date><risdate>2016</risdate><volume>56</volume><issue>2</issue><spage>148</spage><epage>157</epage><pages>148-157</pages><issn>0046-8991</issn><eissn>0973-7715</eissn><abstract>The aim of this study was to purify, characterize and evaluate the antibacterial activity of bioactive compound against methicillin-resistant
Staphylococcus aureus
(MRSA). The anti-MRSA compound was produced by a halophilic bacterial strain designated as MHB1. The MHB1 strain exhibited 99 % similarity to
Bacillus amyloliquefaciens
based on 16S rRNA gene analysis. The culture conditions of
Bacillus amyloliquefaciens
MHB1 were optimized using nutritional and environmental parameters for enhanced anti-MRSA compound production. The pure bioactive compound was isolated using silica gel column chromatography and Semi-preparative High-performance liquid chromatography (Semi-preparative HPLC). The Thin layer chromatography, Fourier transform infrared spectroscopy and proton NMR (
1
H NMR) analysis indicated the phenolic nature of the compound. The molecular mass of the purified compound was 507 Da as revealed by Liquid chromatography-mass spectrometry (LC–MS) analysis. The compound inhibited the growth of MRSA with minimum inhibitory concentration (MIC) of 62.5 µg mL
−1
. MRSA bacteria exposed to 4× MIC of the compound and the cell viability was determined using flow cytometric analysis. Scanning electron microscope and Transmission electron microscope analysis was used to determine the ultrastructural changes in bacteria. This is the first report on isolation of anti-MRSA compound from halophilic
B. amyloliquefaciens
MHB1 and could act as a promising biocontrol agent.</abstract><cop>New Delhi</cop><pub>Springer India</pub><pmid>27570306</pmid><doi>10.1007/s12088-016-0566-8</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Springer Nature - Complete Springer Journals; PubMed Central |
| subjects | antibacterial properties antibiotic resistance Bacillus amyloliquefaciens Bacteria Bioactive compounds Biological control biological control agents Biomedical and Life Sciences cell viability Chromatography Electron microscopes environmental factors Flow cytometry Fourier transform infrared spectroscopy Fourier transforms genes growth retardation high performance liquid chromatography Infrared spectroscopy Laboratories Life Sciences Liquid chromatography Mass spectrometry mechanism of action Medical Microbiology Metabolites methicillin Microbiology minimum inhibitory concentration Mode of action molecular weight nuclear magnetic resonance spectroscopy Original Original Article Pathogens Phenols ribosomal RNA Salinity scanning electron microscopes Silica silica gel Staphylococcus aureus Staphylococcus infections Thin layer chromatography transmission electron microscopes |
| title | Anti-methicillin Resistant Staphylococcus aureus Compound Isolation from Halophilic Bacillus amyloliquefaciens MHB1 and Determination of Its Mode of Action Using Electron Microscope and Flow Cytometry Analysis |
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