High-efficiency CRISPR/Cas9 multiplex gene editing using the glycine tRNA-processing system-based strategy in maize

CRISPR/Cas9 genome editing strategy has been applied to a variety of species and the tRNA-processing system has been used to compact multiple gRNAs into one synthetic gene for manipulating multiple genes in rice. We optimized and introduced the multiplex gene editing strategy based on the tRNA-proce...

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Veröffentlicht in:	BMC biotechnology 2016-08, Vol.16 (1), p.58-58, Article 58
Hauptverfasser:	Qi, Weiwei, Zhu, Tong, Tian, Zhongrui, Li, Chaobin, Zhang, Wei, Song, Rentao
Format:	Artikel
Sprache:	eng
Schlagworte:	breeding Clustered Regularly Interspaced Short Palindromic Repeats Corn CRISPR-Associated Proteins gene editing Gene Editing - methods Gene mutations Genes Genes, Plant - genetics Genetic aspects Glycine - genetics Methodology Methods mutagenesis Mutation non-coding RNA Observations Oryza sativa Physiological aspects Plant Proteins - genetics Plants, Genetically Modified - genetics Plasmids rice RNA sequencing RNA, Transfer - genetics synthetic genes Transfer RNA Zea mays Zea mays - genetics
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