Stability of limbal stem cell deficiency after mechanical and thermal injuries in mice
We studied the reproducibility and stability of limbal stem cell deficiency (LSCD) in mice following controlled injuries to the corneal and limbal epithelia. In one method, corneal and limbal epithelia were entirely removed with a 0.5 mm metal burr. In the other, limbus to limbus epithelial removal...
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| Veröffentlicht in: | Experimental eye research 2016-04, Vol.145, p.88-92 |
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| creator | Afsharkhamseh, Neda Movahedan, Asadolah Gidfar, Sanaz Huvard, Michael Wasielewski, Lisa Milani, Behrad Y. Eslani, Medi Djalilian, Ali R. |
| description | We studied the reproducibility and stability of limbal stem cell deficiency (LSCD) in mice following controlled injuries to the corneal and limbal epithelia. In one method, corneal and limbal epithelia were entirely removed with a 0.5 mm metal burr. In the other, limbus to limbus epithelial removal with the burr was followed by thermal injury to the limbus. These two methods were compared with a previously published one. Unwounded corneas were used as control. The corneas were examined monthly for three months by slit lamp with fluorescein staining. Immunofluorescence staining for cytokeratin 12 and 8 on corneal wholemount and cross sections were performed to determine the phenotype of the epithelium. Mechanical shaving of the epithelium, with or without thermal injury, resulted in a reproducible state of LSCD marked by superficial neovascularization, reduce of keratin 12 expression and presence of goblet cells on the cornea. The phenotype was stable in 100% of the eyes up to at least three months. Thermal injury produced a more severe phenotype with more significant stromal opacification. These corneal injury models may be useful for studying the mechanisms leading to limbal stem cell deficiency. |
| doi_str_mv | 10.1016/j.exer.2015.11.012 |
| format | Article |
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In one method, corneal and limbal epithelia were entirely removed with a 0.5 mm metal burr. In the other, limbus to limbus epithelial removal with the burr was followed by thermal injury to the limbus. These two methods were compared with a previously published one. Unwounded corneas were used as control. The corneas were examined monthly for three months by slit lamp with fluorescein staining. Immunofluorescence staining for cytokeratin 12 and 8 on corneal wholemount and cross sections were performed to determine the phenotype of the epithelium. Mechanical shaving of the epithelium, with or without thermal injury, resulted in a reproducible state of LSCD marked by superficial neovascularization, reduce of keratin 12 expression and presence of goblet cells on the cornea. The phenotype was stable in 100% of the eyes up to at least three months. Thermal injury produced a more severe phenotype with more significant stromal opacification. These corneal injury models may be useful for studying the mechanisms leading to limbal stem cell deficiency.</description><identifier>ISSN: 0014-4835</identifier><identifier>EISSN: 1096-0007</identifier><identifier>DOI: 10.1016/j.exer.2015.11.012</identifier><identifier>PMID: 26607808</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Conjunctivalization ; Cornea ; Corneal Injuries - complications ; Corneal Injuries - pathology ; Corneal Neovascularization - etiology ; Corneal Neovascularization - pathology ; Corneal opacity ; Disease Models, Animal ; Epithelium ; Eye Burns - complications ; Eye Burns - pathology ; Limbal stem cell deficiency ; Limbus ; Limbus Corneae - injuries ; Limbus Corneae - pathology ; Mice ; Mouse model ; Neovascularization ; Stem cell ; Stem Cells - pathology</subject><ispartof>Experimental eye research, 2016-04, Vol.145, p.88-92</ispartof><rights>2015 Elsevier Ltd</rights><rights>Copyright © 2015 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-600acc15e711fdaf1af6e54c099c93bbfbb5f43aa2807f046981ffca57a743223</citedby><cites>FETCH-LOGICAL-c455t-600acc15e711fdaf1af6e54c099c93bbfbb5f43aa2807f046981ffca57a743223</cites><orcidid>0000-0001-5759-6579 ; 0000-0002-2625-7432 ; 0000-0003-3647-0250</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014483515300701$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3536,27902,27903,65308</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26607808$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Afsharkhamseh, Neda</creatorcontrib><creatorcontrib>Movahedan, Asadolah</creatorcontrib><creatorcontrib>Gidfar, Sanaz</creatorcontrib><creatorcontrib>Huvard, Michael</creatorcontrib><creatorcontrib>Wasielewski, Lisa</creatorcontrib><creatorcontrib>Milani, Behrad Y.</creatorcontrib><creatorcontrib>Eslani, Medi</creatorcontrib><creatorcontrib>Djalilian, Ali R.</creatorcontrib><title>Stability of limbal stem cell deficiency after mechanical and thermal injuries in mice</title><title>Experimental eye research</title><addtitle>Exp Eye Res</addtitle><description>We studied the reproducibility and stability of limbal stem cell deficiency (LSCD) in mice following controlled injuries to the corneal and limbal epithelia. In one method, corneal and limbal epithelia were entirely removed with a 0.5 mm metal burr. In the other, limbus to limbus epithelial removal with the burr was followed by thermal injury to the limbus. These two methods were compared with a previously published one. Unwounded corneas were used as control. The corneas were examined monthly for three months by slit lamp with fluorescein staining. Immunofluorescence staining for cytokeratin 12 and 8 on corneal wholemount and cross sections were performed to determine the phenotype of the epithelium. Mechanical shaving of the epithelium, with or without thermal injury, resulted in a reproducible state of LSCD marked by superficial neovascularization, reduce of keratin 12 expression and presence of goblet cells on the cornea. The phenotype was stable in 100% of the eyes up to at least three months. Thermal injury produced a more severe phenotype with more significant stromal opacification. These corneal injury models may be useful for studying the mechanisms leading to limbal stem cell deficiency.</description><subject>Animals</subject><subject>Conjunctivalization</subject><subject>Cornea</subject><subject>Corneal Injuries - complications</subject><subject>Corneal Injuries - pathology</subject><subject>Corneal Neovascularization - etiology</subject><subject>Corneal Neovascularization - pathology</subject><subject>Corneal opacity</subject><subject>Disease Models, Animal</subject><subject>Epithelium</subject><subject>Eye Burns - complications</subject><subject>Eye Burns - pathology</subject><subject>Limbal stem cell deficiency</subject><subject>Limbus</subject><subject>Limbus Corneae - injuries</subject><subject>Limbus Corneae - pathology</subject><subject>Mice</subject><subject>Mouse model</subject><subject>Neovascularization</subject><subject>Stem cell</subject><subject>Stem Cells - pathology</subject><issn>0014-4835</issn><issn>1096-0007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhi0EotvCH-CAfOSSMJM4cSIhJFQBRarEgY-rNXHGrFf5KLa36v57vGyp4MLJI8_zvvbMK8QLhBIB29e7ku84lBVgUyKWgNUjsUHo2wIA9GOxAUBVqK5uzsR5jLt8Wyutnoqzqm1Bd9BtxPcviQY_-XSQq5OTnweaZEw8S8vTJEd23npe7EGSSxzkzHZLi7eZomWUacthzrVfdvvgOeZCzt7yM_HE0RT5-f15Ib59eP_18qq4_vzx0-W768KqpklFC0DWYsMa0Y3kkFzLjbLQ97avh8ENQ-NUTVR1oB2otu_QOUuNJq3qqqovxNuT781-mHm0vKRAk7kJfqZwMCt5829n8VvzY701qtd9CyobvLo3COvPPcdkZh-Po9PC6z4a1J2CTqsOM1qdUBvWGAO7h2cQzDEQszPHQMwxEINociBZ9PLvDz5I_iSQgTcngPOabn2Wx98L59EHtsmMq_-f_y_q5Z6X</recordid><startdate>20160401</startdate><enddate>20160401</enddate><creator>Afsharkhamseh, Neda</creator><creator>Movahedan, Asadolah</creator><creator>Gidfar, Sanaz</creator><creator>Huvard, Michael</creator><creator>Wasielewski, Lisa</creator><creator>Milani, Behrad Y.</creator><creator>Eslani, Medi</creator><creator>Djalilian, Ali R.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5759-6579</orcidid><orcidid>https://orcid.org/0000-0002-2625-7432</orcidid><orcidid>https://orcid.org/0000-0003-3647-0250</orcidid></search><sort><creationdate>20160401</creationdate><title>Stability of limbal stem cell deficiency after mechanical and thermal injuries in mice</title><author>Afsharkhamseh, Neda ; Movahedan, Asadolah ; Gidfar, Sanaz ; Huvard, Michael ; Wasielewski, Lisa ; Milani, Behrad Y. ; Eslani, Medi ; Djalilian, Ali R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-600acc15e711fdaf1af6e54c099c93bbfbb5f43aa2807f046981ffca57a743223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>Conjunctivalization</topic><topic>Cornea</topic><topic>Corneal Injuries - complications</topic><topic>Corneal Injuries - pathology</topic><topic>Corneal Neovascularization - etiology</topic><topic>Corneal Neovascularization - pathology</topic><topic>Corneal opacity</topic><topic>Disease Models, Animal</topic><topic>Epithelium</topic><topic>Eye Burns - complications</topic><topic>Eye Burns - pathology</topic><topic>Limbal stem cell deficiency</topic><topic>Limbus</topic><topic>Limbus Corneae - injuries</topic><topic>Limbus Corneae - pathology</topic><topic>Mice</topic><topic>Mouse model</topic><topic>Neovascularization</topic><topic>Stem cell</topic><topic>Stem Cells - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Afsharkhamseh, Neda</creatorcontrib><creatorcontrib>Movahedan, Asadolah</creatorcontrib><creatorcontrib>Gidfar, Sanaz</creatorcontrib><creatorcontrib>Huvard, Michael</creatorcontrib><creatorcontrib>Wasielewski, Lisa</creatorcontrib><creatorcontrib>Milani, Behrad Y.</creatorcontrib><creatorcontrib>Eslani, Medi</creatorcontrib><creatorcontrib>Djalilian, Ali R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Afsharkhamseh, Neda</au><au>Movahedan, Asadolah</au><au>Gidfar, Sanaz</au><au>Huvard, Michael</au><au>Wasielewski, Lisa</au><au>Milani, Behrad Y.</au><au>Eslani, Medi</au><au>Djalilian, Ali R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stability of limbal stem cell deficiency after mechanical and thermal injuries in mice</atitle><jtitle>Experimental eye research</jtitle><addtitle>Exp Eye Res</addtitle><date>2016-04-01</date><risdate>2016</risdate><volume>145</volume><spage>88</spage><epage>92</epage><pages>88-92</pages><issn>0014-4835</issn><eissn>1096-0007</eissn><abstract>We studied the reproducibility and stability of limbal stem cell deficiency (LSCD) in mice following controlled injuries to the corneal and limbal epithelia. In one method, corneal and limbal epithelia were entirely removed with a 0.5 mm metal burr. In the other, limbus to limbus epithelial removal with the burr was followed by thermal injury to the limbus. These two methods were compared with a previously published one. Unwounded corneas were used as control. The corneas were examined monthly for three months by slit lamp with fluorescein staining. Immunofluorescence staining for cytokeratin 12 and 8 on corneal wholemount and cross sections were performed to determine the phenotype of the epithelium. Mechanical shaving of the epithelium, with or without thermal injury, resulted in a reproducible state of LSCD marked by superficial neovascularization, reduce of keratin 12 expression and presence of goblet cells on the cornea. The phenotype was stable in 100% of the eyes up to at least three months. Thermal injury produced a more severe phenotype with more significant stromal opacification. 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| subjects | Animals Conjunctivalization Cornea Corneal Injuries - complications Corneal Injuries - pathology Corneal Neovascularization - etiology Corneal Neovascularization - pathology Corneal opacity Disease Models, Animal Epithelium Eye Burns - complications Eye Burns - pathology Limbal stem cell deficiency Limbus Limbus Corneae - injuries Limbus Corneae - pathology Mice Mouse model Neovascularization Stem cell Stem Cells - pathology |
| title | Stability of limbal stem cell deficiency after mechanical and thermal injuries in mice |
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