Cardiorespiratory fitness as a predictor of intestinal microbial diversity and distinct metagenomic functions
Reduced microbial diversity in human intestines has been implicated in various conditions such as diabetes, colorectal cancer, and inflammatory bowel disease. The role of physical fitness in the context of human intestinal microbiota is currently not known. We used high-throughput sequencing to anal...
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| Veröffentlicht in: | Microbiome 2016-08, Vol.4 (1), p.42-42, Article 42 |
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| creator | Estaki, Mehrbod Pither, Jason Baumeister, Peter Little, Jonathan P Gill, Sandeep K Ghosh, Sanjoy Ahmadi-Vand, Zahra Marsden, Katelyn R Gibson, Deanna L |
| description | Reduced microbial diversity in human intestines has been implicated in various conditions such as diabetes, colorectal cancer, and inflammatory bowel disease. The role of physical fitness in the context of human intestinal microbiota is currently not known. We used high-throughput sequencing to analyze fecal microbiota of 39 healthy participants with similar age, BMI, and diets but with varying cardiorespiratory fitness levels. Fecal short-chain fatty acids were analyzed using gas chromatography.
We showed that peak oxygen uptake (VO2peak), the gold standard measure of cardiorespiratory fitness, can account for more than 20 % of the variation in taxonomic richness, after accounting for all other factors, including diet. While VO2peak did not explain variation in beta diversity, it did play a significant role in explaining variation in the microbiomes' predicted metagenomic functions, aligning positively with genes related to bacterial chemotaxis, motility, and fatty acid biosynthesis. These predicted functions were supported by measured increases in production of fecal butyrate, a short-chain fatty acid associated with improved gut health, amongst physically fit participants. We also identified increased abundances of key butyrate-producing taxa (Clostridiales, Roseburia, Lachnospiraceae, and Erysipelotrichaceae) amongst these individuals, which likely contributed to the observed increases in butyrate levels.
Results from this study show that cardiorespiratory fitness is correlated with increased microbial diversity in healthy humans and that the associated changes are anchored around a set of functional cores rather than specific taxa. The microbial profiles of fit individuals favor the production of butyrate. As increased microbiota diversity and butyrate production is associated with overall host health, our findings warrant the use of exercise prescription as an adjuvant therapy in combating dysbiosis-associated diseases. |
| doi_str_mv | 10.1186/s40168-016-0189-7 |
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We showed that peak oxygen uptake (VO2peak), the gold standard measure of cardiorespiratory fitness, can account for more than 20 % of the variation in taxonomic richness, after accounting for all other factors, including diet. While VO2peak did not explain variation in beta diversity, it did play a significant role in explaining variation in the microbiomes' predicted metagenomic functions, aligning positively with genes related to bacterial chemotaxis, motility, and fatty acid biosynthesis. These predicted functions were supported by measured increases in production of fecal butyrate, a short-chain fatty acid associated with improved gut health, amongst physically fit participants. We also identified increased abundances of key butyrate-producing taxa (Clostridiales, Roseburia, Lachnospiraceae, and Erysipelotrichaceae) amongst these individuals, which likely contributed to the observed increases in butyrate levels.
Results from this study show that cardiorespiratory fitness is correlated with increased microbial diversity in healthy humans and that the associated changes are anchored around a set of functional cores rather than specific taxa. The microbial profiles of fit individuals favor the production of butyrate. As increased microbiota diversity and butyrate production is associated with overall host health, our findings warrant the use of exercise prescription as an adjuvant therapy in combating dysbiosis-associated diseases.</description><identifier>ISSN: 2049-2618</identifier><identifier>EISSN: 2049-2618</identifier><identifier>DOI: 10.1186/s40168-016-0189-7</identifier><identifier>PMID: 27502158</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Adult ; Analysis ; Bacteria - classification ; Bacteria - genetics ; Bacteria - isolation & purification ; Base Sequence ; Biological diversity ; Body mass index ; Butyrates - isolation & purification ; Cardiorespiratory Fitness - physiology ; Chromatography ; Chromatography, Gas ; Colorectal cancer ; Esters ; Exercise - physiology ; Exercise therapy ; Fatty acids ; Fatty Acids, Volatile - analysis ; Feces - chemistry ; Feces - microbiology ; Female ; Gastrointestinal diseases ; Gastrointestinal Microbiome - genetics ; High-Throughput Nucleotide Sequencing ; Humans ; Intestines - microbiology ; Male ; Metagenome - genetics ; Microbiota (Symbiotic organisms) ; Physiological aspects ; Pulmonary Gas Exchange ; Sequence Analysis, DNA ; Young Adult</subject><ispartof>Microbiome, 2016-08, Vol.4 (1), p.42-42, Article 42</ispartof><rights>COPYRIGHT 2016 BioMed Central Ltd.</rights><rights>The Author(s). 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c566t-69f195196eced76463aeaec7fa0c1b0da9bfda5ae0e223477575af656d88381d3</citedby><cites>FETCH-LOGICAL-c566t-69f195196eced76463aeaec7fa0c1b0da9bfda5ae0e223477575af656d88381d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4976518/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4976518/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27502158$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Estaki, Mehrbod</creatorcontrib><creatorcontrib>Pither, Jason</creatorcontrib><creatorcontrib>Baumeister, Peter</creatorcontrib><creatorcontrib>Little, Jonathan P</creatorcontrib><creatorcontrib>Gill, Sandeep K</creatorcontrib><creatorcontrib>Ghosh, Sanjoy</creatorcontrib><creatorcontrib>Ahmadi-Vand, Zahra</creatorcontrib><creatorcontrib>Marsden, Katelyn R</creatorcontrib><creatorcontrib>Gibson, Deanna L</creatorcontrib><title>Cardiorespiratory fitness as a predictor of intestinal microbial diversity and distinct metagenomic functions</title><title>Microbiome</title><addtitle>Microbiome</addtitle><description>Reduced microbial diversity in human intestines has been implicated in various conditions such as diabetes, colorectal cancer, and inflammatory bowel disease. The role of physical fitness in the context of human intestinal microbiota is currently not known. We used high-throughput sequencing to analyze fecal microbiota of 39 healthy participants with similar age, BMI, and diets but with varying cardiorespiratory fitness levels. Fecal short-chain fatty acids were analyzed using gas chromatography.
We showed that peak oxygen uptake (VO2peak), the gold standard measure of cardiorespiratory fitness, can account for more than 20 % of the variation in taxonomic richness, after accounting for all other factors, including diet. While VO2peak did not explain variation in beta diversity, it did play a significant role in explaining variation in the microbiomes' predicted metagenomic functions, aligning positively with genes related to bacterial chemotaxis, motility, and fatty acid biosynthesis. These predicted functions were supported by measured increases in production of fecal butyrate, a short-chain fatty acid associated with improved gut health, amongst physically fit participants. We also identified increased abundances of key butyrate-producing taxa (Clostridiales, Roseburia, Lachnospiraceae, and Erysipelotrichaceae) amongst these individuals, which likely contributed to the observed increases in butyrate levels.
Results from this study show that cardiorespiratory fitness is correlated with increased microbial diversity in healthy humans and that the associated changes are anchored around a set of functional cores rather than specific taxa. The microbial profiles of fit individuals favor the production of butyrate. As increased microbiota diversity and butyrate production is associated with overall host health, our findings warrant the use of exercise prescription as an adjuvant therapy in combating dysbiosis-associated diseases.</description><subject>Adult</subject><subject>Analysis</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Base Sequence</subject><subject>Biological diversity</subject><subject>Body mass index</subject><subject>Butyrates - isolation & purification</subject><subject>Cardiorespiratory Fitness - physiology</subject><subject>Chromatography</subject><subject>Chromatography, Gas</subject><subject>Colorectal cancer</subject><subject>Esters</subject><subject>Exercise - physiology</subject><subject>Exercise therapy</subject><subject>Fatty acids</subject><subject>Fatty Acids, Volatile - analysis</subject><subject>Feces - chemistry</subject><subject>Feces - microbiology</subject><subject>Female</subject><subject>Gastrointestinal diseases</subject><subject>Gastrointestinal Microbiome - genetics</subject><subject>High-Throughput Nucleotide Sequencing</subject><subject>Humans</subject><subject>Intestines - microbiology</subject><subject>Male</subject><subject>Metagenome - genetics</subject><subject>Microbiota (Symbiotic organisms)</subject><subject>Physiological aspects</subject><subject>Pulmonary Gas Exchange</subject><subject>Sequence Analysis, DNA</subject><subject>Young Adult</subject><issn>2049-2618</issn><issn>2049-2618</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkllr3DAQgE1paUKaH9CXYuhL--BUsq3DL4Ww9AgECj2exaw12qrY0laSQ_ffd8ymIQvVrdE3g-aoqpecXXGu5bvcMy51QwtNPTTqSXXesn5oWsn100fns-oy51-M2sB71evn1VmrBGu50OfVvIFkfUyY9z5BielQO18C5lwDjXqf0PqR5HV0tQ8Fc_EBpnr2Y4pbTyfr7zBlXw41BEu3FRhLPWOBHYZIYO0WkvgY8ovqmYMp4-X9flH9-Pjh--Zzc_vl083m-rYZhZSlkYPjg-CDxBGtkr3sAAFH5YCNfMssDFtnQQAybNuuV0ooAU4KabXuNLfdRfX-aHe_bGe0I4aSYDL75GdIBxPBm9OX4H-aXbwz_aCk4JoMvLk3kOLvhZw2s88jThMEjEs2XHMmxNByRejrI7qDCY0PLpLFccXNdS-15pSClqir_1DULVKEYkDnSX6i8PZEgZiCf8oOlpzNzbevpyw_spSTnBO6B085M2uxmGOxGFrMWixm_farxyF60PhXGt1fsWC8Nw</recordid><startdate>20160808</startdate><enddate>20160808</enddate><creator>Estaki, Mehrbod</creator><creator>Pither, Jason</creator><creator>Baumeister, Peter</creator><creator>Little, Jonathan P</creator><creator>Gill, Sandeep K</creator><creator>Ghosh, Sanjoy</creator><creator>Ahmadi-Vand, Zahra</creator><creator>Marsden, Katelyn R</creator><creator>Gibson, Deanna L</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160808</creationdate><title>Cardiorespiratory fitness as a predictor of intestinal microbial diversity and distinct metagenomic functions</title><author>Estaki, Mehrbod ; Pither, Jason ; Baumeister, Peter ; Little, Jonathan P ; Gill, Sandeep K ; Ghosh, Sanjoy ; Ahmadi-Vand, Zahra ; Marsden, Katelyn R ; Gibson, Deanna L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c566t-69f195196eced76463aeaec7fa0c1b0da9bfda5ae0e223477575af656d88381d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Adult</topic><topic>Analysis</topic><topic>Bacteria - classification</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation & purification</topic><topic>Base Sequence</topic><topic>Biological diversity</topic><topic>Body mass index</topic><topic>Butyrates - isolation & purification</topic><topic>Cardiorespiratory Fitness - physiology</topic><topic>Chromatography</topic><topic>Chromatography, Gas</topic><topic>Colorectal cancer</topic><topic>Esters</topic><topic>Exercise - physiology</topic><topic>Exercise therapy</topic><topic>Fatty acids</topic><topic>Fatty Acids, Volatile - analysis</topic><topic>Feces - chemistry</topic><topic>Feces - microbiology</topic><topic>Female</topic><topic>Gastrointestinal diseases</topic><topic>Gastrointestinal Microbiome - genetics</topic><topic>High-Throughput Nucleotide Sequencing</topic><topic>Humans</topic><topic>Intestines - microbiology</topic><topic>Male</topic><topic>Metagenome - genetics</topic><topic>Microbiota (Symbiotic organisms)</topic><topic>Physiological aspects</topic><topic>Pulmonary Gas Exchange</topic><topic>Sequence Analysis, DNA</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Estaki, Mehrbod</creatorcontrib><creatorcontrib>Pither, Jason</creatorcontrib><creatorcontrib>Baumeister, Peter</creatorcontrib><creatorcontrib>Little, Jonathan P</creatorcontrib><creatorcontrib>Gill, Sandeep K</creatorcontrib><creatorcontrib>Ghosh, Sanjoy</creatorcontrib><creatorcontrib>Ahmadi-Vand, Zahra</creatorcontrib><creatorcontrib>Marsden, Katelyn R</creatorcontrib><creatorcontrib>Gibson, Deanna L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Microbiome</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Estaki, Mehrbod</au><au>Pither, Jason</au><au>Baumeister, Peter</au><au>Little, Jonathan P</au><au>Gill, Sandeep K</au><au>Ghosh, Sanjoy</au><au>Ahmadi-Vand, Zahra</au><au>Marsden, Katelyn R</au><au>Gibson, Deanna L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cardiorespiratory fitness as a predictor of intestinal microbial diversity and distinct metagenomic functions</atitle><jtitle>Microbiome</jtitle><addtitle>Microbiome</addtitle><date>2016-08-08</date><risdate>2016</risdate><volume>4</volume><issue>1</issue><spage>42</spage><epage>42</epage><pages>42-42</pages><artnum>42</artnum><issn>2049-2618</issn><eissn>2049-2618</eissn><abstract>Reduced microbial diversity in human intestines has been implicated in various conditions such as diabetes, colorectal cancer, and inflammatory bowel disease. The role of physical fitness in the context of human intestinal microbiota is currently not known. We used high-throughput sequencing to analyze fecal microbiota of 39 healthy participants with similar age, BMI, and diets but with varying cardiorespiratory fitness levels. Fecal short-chain fatty acids were analyzed using gas chromatography.
We showed that peak oxygen uptake (VO2peak), the gold standard measure of cardiorespiratory fitness, can account for more than 20 % of the variation in taxonomic richness, after accounting for all other factors, including diet. While VO2peak did not explain variation in beta diversity, it did play a significant role in explaining variation in the microbiomes' predicted metagenomic functions, aligning positively with genes related to bacterial chemotaxis, motility, and fatty acid biosynthesis. These predicted functions were supported by measured increases in production of fecal butyrate, a short-chain fatty acid associated with improved gut health, amongst physically fit participants. We also identified increased abundances of key butyrate-producing taxa (Clostridiales, Roseburia, Lachnospiraceae, and Erysipelotrichaceae) amongst these individuals, which likely contributed to the observed increases in butyrate levels.
Results from this study show that cardiorespiratory fitness is correlated with increased microbial diversity in healthy humans and that the associated changes are anchored around a set of functional cores rather than specific taxa. The microbial profiles of fit individuals favor the production of butyrate. As increased microbiota diversity and butyrate production is associated with overall host health, our findings warrant the use of exercise prescription as an adjuvant therapy in combating dysbiosis-associated diseases.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>27502158</pmid><doi>10.1186/s40168-016-0189-7</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Analysis Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Base Sequence Biological diversity Body mass index Butyrates - isolation & purification Cardiorespiratory Fitness - physiology Chromatography Chromatography, Gas Colorectal cancer Esters Exercise - physiology Exercise therapy Fatty acids Fatty Acids, Volatile - analysis Feces - chemistry Feces - microbiology Female Gastrointestinal diseases Gastrointestinal Microbiome - genetics High-Throughput Nucleotide Sequencing Humans Intestines - microbiology Male Metagenome - genetics Microbiota (Symbiotic organisms) Physiological aspects Pulmonary Gas Exchange Sequence Analysis, DNA Young Adult |
| title | Cardiorespiratory fitness as a predictor of intestinal microbial diversity and distinct metagenomic functions |
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