Rescuing failed oral implants via Wnt activation
Aim Implant osseointegration is not always guaranteed and once fibrous encapsulation occurs clinicians have few options other than implant removal. Our goal was to test whether a WNT protein therapeutic could rescue such failed implants. Material and Methods Titanium implants were placed in over‐siz...
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| Veröffentlicht in: | Journal of clinical periodontology 2016-02, Vol.43 (2), p.180-192 |
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| container_title | Journal of clinical periodontology |
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| creator | Yin, Xing Li, Jingtao Chen, Tao Mouraret, Sylvain Dhamdhere, Girija Brunski, John B. Zou, Shujuan Helms, Jill A. |
| description | Aim
Implant osseointegration is not always guaranteed and once fibrous encapsulation occurs clinicians have few options other than implant removal. Our goal was to test whether a WNT protein therapeutic could rescue such failed implants.
Material and Methods
Titanium implants were placed in over‐sized murine oral osteotomies. A lack of primary stability was verified by mechanical testing. Interfacial strains were estimated by finite element modelling and histology coupled with histomorphometry confirmed the lack of peri‐implant bone. After fibrous encapsulation was established peri‐implant injections of a liposomal formulation of WNT3A protein (L‐WNT3A) or liposomal PBS (L‐PBS) were then initiated. Quantitative assays were employed to analyse the effects of L‐WNT3A treatment.
Results
Implants in gap‐type interfaces exhibited high interfacial strains and no primary stability. After verification of implant failure, L‐WNT3A or L‐PBS injections were initiated. L‐WNT3A induced a rapid, significant increase in Wnt responsiveness in the peri‐implant environment, cell proliferation and osteogenic protein expression. The amount of peri‐implant bone and bone in contact with the implant were significantly higher in L‐WNT3A cases.
Conclusions
These data demonstrate L‐WNT3A can induce peri‐implant bone formation even in cases where fibrous encapsulation predominates. |
| doi_str_mv | 10.1111/jcpe.12503 |
| format | Article |
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Implant osseointegration is not always guaranteed and once fibrous encapsulation occurs clinicians have few options other than implant removal. Our goal was to test whether a WNT protein therapeutic could rescue such failed implants.
Material and Methods
Titanium implants were placed in over‐sized murine oral osteotomies. A lack of primary stability was verified by mechanical testing. Interfacial strains were estimated by finite element modelling and histology coupled with histomorphometry confirmed the lack of peri‐implant bone. After fibrous encapsulation was established peri‐implant injections of a liposomal formulation of WNT3A protein (L‐WNT3A) or liposomal PBS (L‐PBS) were then initiated. Quantitative assays were employed to analyse the effects of L‐WNT3A treatment.
Results
Implants in gap‐type interfaces exhibited high interfacial strains and no primary stability. After verification of implant failure, L‐WNT3A or L‐PBS injections were initiated. L‐WNT3A induced a rapid, significant increase in Wnt responsiveness in the peri‐implant environment, cell proliferation and osteogenic protein expression. The amount of peri‐implant bone and bone in contact with the implant were significantly higher in L‐WNT3A cases.
Conclusions
These data demonstrate L‐WNT3A can induce peri‐implant bone formation even in cases where fibrous encapsulation predominates.</description><identifier>ISSN: 0303-6979</identifier><identifier>EISSN: 1600-051X</identifier><identifier>DOI: 10.1111/jcpe.12503</identifier><identifier>PMID: 26718012</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Animals ; biomechanics ; Dental Implants ; Dentistry ; fibrosis ; finite element analysis ; Male ; Mice ; Osseointegration ; Osteogenesis ; Proteins ; Surface Properties ; Titanium ; Transplants & implants ; Wnt Proteins</subject><ispartof>Journal of clinical periodontology, 2016-02, Vol.43 (2), p.180-192</ispartof><rights>2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4343-5812e14e2ddc9513ba818bdb2e8a9b82086a0060d803120aa0f98b55b6a408213</citedby><cites>FETCH-LOGICAL-c4343-5812e14e2ddc9513ba818bdb2e8a9b82086a0060d803120aa0f98b55b6a408213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjcpe.12503$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjcpe.12503$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26718012$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yin, Xing</creatorcontrib><creatorcontrib>Li, Jingtao</creatorcontrib><creatorcontrib>Chen, Tao</creatorcontrib><creatorcontrib>Mouraret, Sylvain</creatorcontrib><creatorcontrib>Dhamdhere, Girija</creatorcontrib><creatorcontrib>Brunski, John B.</creatorcontrib><creatorcontrib>Zou, Shujuan</creatorcontrib><creatorcontrib>Helms, Jill A.</creatorcontrib><title>Rescuing failed oral implants via Wnt activation</title><title>Journal of clinical periodontology</title><addtitle>J Clin Periodontol</addtitle><description>Aim
Implant osseointegration is not always guaranteed and once fibrous encapsulation occurs clinicians have few options other than implant removal. Our goal was to test whether a WNT protein therapeutic could rescue such failed implants.
Material and Methods
Titanium implants were placed in over‐sized murine oral osteotomies. A lack of primary stability was verified by mechanical testing. Interfacial strains were estimated by finite element modelling and histology coupled with histomorphometry confirmed the lack of peri‐implant bone. After fibrous encapsulation was established peri‐implant injections of a liposomal formulation of WNT3A protein (L‐WNT3A) or liposomal PBS (L‐PBS) were then initiated. Quantitative assays were employed to analyse the effects of L‐WNT3A treatment.
Results
Implants in gap‐type interfaces exhibited high interfacial strains and no primary stability. After verification of implant failure, L‐WNT3A or L‐PBS injections were initiated. L‐WNT3A induced a rapid, significant increase in Wnt responsiveness in the peri‐implant environment, cell proliferation and osteogenic protein expression. The amount of peri‐implant bone and bone in contact with the implant were significantly higher in L‐WNT3A cases.
Conclusions
These data demonstrate L‐WNT3A can induce peri‐implant bone formation even in cases where fibrous encapsulation predominates.</description><subject>Animals</subject><subject>biomechanics</subject><subject>Dental Implants</subject><subject>Dentistry</subject><subject>fibrosis</subject><subject>finite element analysis</subject><subject>Male</subject><subject>Mice</subject><subject>Osseointegration</subject><subject>Osteogenesis</subject><subject>Proteins</subject><subject>Surface Properties</subject><subject>Titanium</subject><subject>Transplants & implants</subject><subject>Wnt Proteins</subject><issn>0303-6979</issn><issn>1600-051X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctO3DAUhq2qqEyHbvoAKFI3FVKm59jxbVOpGgFlQEBvgp3lJB7wNJMMcTItb8Oz8GSYDoygi9YbL_ydX-f3R8hbhBHG82FWLNwIKQf2ggxQAKTA8fwlGQADlgot9SZ5HcIMACVj7BXZpEKiAqQDgl9dKHpfXyRT6ytXJk1rq8TPF5Wtu5Asvb29Oau7xBadX9rON_UW2ZjaKrg3D_eQ_Njb_T7-nB6d7B-MPx2lRcYylnKF1GHmaFkWmiPLrUKVlzl1yupcUVDCAggoFTCkYC1Mtco5z4XNQFFkQ_Jxlbvo87krC1d3cTWzaP3cttemsd48f6n9pblolibTkgmqYsD7h4C2uepd6Mzch8JVsZlr-mBQo9Yiy5D9H5USKGrJeUTf_YXOmr6t409ESugMUMdGQ7Kzooq2CaF10_XeCOZemrmXZv5Ii_D206Zr9NFSBHAF_IqKrv8RZSbj093H0HQ140Pnfq9nbPvTCMkkN2fH-0ZO9Je988NvhrI7wCavvw</recordid><startdate>201602</startdate><enddate>201602</enddate><creator>Yin, Xing</creator><creator>Li, Jingtao</creator><creator>Chen, Tao</creator><creator>Mouraret, Sylvain</creator><creator>Dhamdhere, Girija</creator><creator>Brunski, John B.</creator><creator>Zou, Shujuan</creator><creator>Helms, Jill A.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>201602</creationdate><title>Rescuing failed oral implants via Wnt activation</title><author>Yin, Xing ; Li, Jingtao ; Chen, Tao ; Mouraret, Sylvain ; Dhamdhere, Girija ; Brunski, John B. ; Zou, Shujuan ; Helms, Jill A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4343-5812e14e2ddc9513ba818bdb2e8a9b82086a0060d803120aa0f98b55b6a408213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Animals</topic><topic>biomechanics</topic><topic>Dental Implants</topic><topic>Dentistry</topic><topic>fibrosis</topic><topic>finite element analysis</topic><topic>Male</topic><topic>Mice</topic><topic>Osseointegration</topic><topic>Osteogenesis</topic><topic>Proteins</topic><topic>Surface Properties</topic><topic>Titanium</topic><topic>Transplants & implants</topic><topic>Wnt Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yin, Xing</creatorcontrib><creatorcontrib>Li, Jingtao</creatorcontrib><creatorcontrib>Chen, Tao</creatorcontrib><creatorcontrib>Mouraret, Sylvain</creatorcontrib><creatorcontrib>Dhamdhere, Girija</creatorcontrib><creatorcontrib>Brunski, John B.</creatorcontrib><creatorcontrib>Zou, Shujuan</creatorcontrib><creatorcontrib>Helms, Jill A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical periodontology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yin, Xing</au><au>Li, Jingtao</au><au>Chen, Tao</au><au>Mouraret, Sylvain</au><au>Dhamdhere, Girija</au><au>Brunski, John B.</au><au>Zou, Shujuan</au><au>Helms, Jill A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rescuing failed oral implants via Wnt activation</atitle><jtitle>Journal of clinical periodontology</jtitle><addtitle>J Clin Periodontol</addtitle><date>2016-02</date><risdate>2016</risdate><volume>43</volume><issue>2</issue><spage>180</spage><epage>192</epage><pages>180-192</pages><issn>0303-6979</issn><eissn>1600-051X</eissn><abstract>Aim
Implant osseointegration is not always guaranteed and once fibrous encapsulation occurs clinicians have few options other than implant removal. Our goal was to test whether a WNT protein therapeutic could rescue such failed implants.
Material and Methods
Titanium implants were placed in over‐sized murine oral osteotomies. A lack of primary stability was verified by mechanical testing. Interfacial strains were estimated by finite element modelling and histology coupled with histomorphometry confirmed the lack of peri‐implant bone. After fibrous encapsulation was established peri‐implant injections of a liposomal formulation of WNT3A protein (L‐WNT3A) or liposomal PBS (L‐PBS) were then initiated. Quantitative assays were employed to analyse the effects of L‐WNT3A treatment.
Results
Implants in gap‐type interfaces exhibited high interfacial strains and no primary stability. After verification of implant failure, L‐WNT3A or L‐PBS injections were initiated. L‐WNT3A induced a rapid, significant increase in Wnt responsiveness in the peri‐implant environment, cell proliferation and osteogenic protein expression. The amount of peri‐implant bone and bone in contact with the implant were significantly higher in L‐WNT3A cases.
Conclusions
These data demonstrate L‐WNT3A can induce peri‐implant bone formation even in cases where fibrous encapsulation predominates.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>26718012</pmid><doi>10.1111/jcpe.12503</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of clinical periodontology, 2016-02, Vol.43 (2), p.180-192 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals biomechanics Dental Implants Dentistry fibrosis finite element analysis Male Mice Osseointegration Osteogenesis Proteins Surface Properties Titanium Transplants & implants Wnt Proteins |
| title | Rescuing failed oral implants via Wnt activation |
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